Rh3BG003500

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000944
Physical Location & Seq
Reverse (-)
302539 .. 304340
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG003500.1

Sequence Viewer

Length: 582 bp
ATGGGACTGGAATTGCAGGGTCCAAAATCCCTTGTGGGCTTTGTTCTTCTGCTCCTCCTTGCTGTGATCGGCGCCGTGGCCCCCAAATCCAGCAGCAAAACAAGCATCGGAGGAGGAAGCATCTACGATCACCTCCGGCAGTATGGCTTGCCCATCGGCCTCCTCCCCAAAGGCATCACGGAATATTCCCTGGACGATTCCAGCGGCGAATTCCGGGTGTTTCTGCCCCAGCCTTGCCGCGCCAAGTTCGAGAATCAGGTCCGCTACGATTTCAACGTCTCCGGGACTCTCACTTTCGGCCGAATCGCCGACTTGTCCGGCGTCTCCGCTCAGGAGCTCTTCCTTTGGTTTCCGGTGAAAGGTATCCGAGTGGATGTGCCCAGCTCCGGCCTCATTTACTTCGATGTCGGCGTTGTCGACAAGCAGTTCTCTTTGTCCCTCTTCGAGTCTCCGCCCGATTGCACTGCTCTTGATCCTTCTGATCCCTCCTCCTCCTCCTCCTCTTCTTCTTCTTCCAATTCCCCATCTGAGCTCAAGCTCAAGACTCTATCGGAAGATGTTGGGTACAAAGCTGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

193

Amino Acids

20.56

Weight (kDa)

4.98

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 41 - 149 2.7e-34 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccBSI CCGCTC 1 cut(s) 329
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 240
AciI CCGC 5 cut(s) 204, 238, 262, 327, 452
AclWI GGATC 2 cut(s) 467, 476
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 1 cut(s) 209
AcyI GRCGYC 2 cut(s) 72, 321
AfaI GTAC 1 cut(s) 566
AfiI CCNNNNNNNGG 2 cut(s) 359, 386
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 5 cut(s) 337, 384, 532, 538, 572
AluI AGCT 5 cut(s) 337, 384, 532, 538, 572
Alw21I GWGCWC 2 cut(s) 339, 534
Alw26I GTCTC 3 cut(s) 283, 328, 453
AlwI GGATC 2 cut(s) 467, 476
AoxI GGCC 4 cut(s) 78, 157, 298, 388
ApeKI GCWGC 2 cut(s) 93, 572
ApoI RAATTY 1 cut(s) 209
AspLEI GCGC 2 cut(s) 74, 242
AspS9I GGNCC 3 cut(s) 20, 79, 259
AsuC2I CCSGG 2 cut(s) 215, 283
AsuHPI GGTGA 2 cut(s) 122, 367
AvaII GGWCC 2 cut(s) 20, 259
BaeGI GKGCMC 1 cut(s) 381
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 339, 534
Bbv12I GWGCWC 2 cut(s) 339, 534
BbvI GCAGC 2 cut(s) 105, 559
BccI CCATC 2 cut(s) 161, 532
BceAI ACGGC 1 cut(s) 59
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 374
BcnI CCSGG 2 cut(s) 215, 283
BcoDI GTCTC 3 cut(s) 283, 328, 453
BfoI RGCGCY 1 cut(s) 75
BfuI GTATCC 1 cut(s) 374
BisI GCNGC 4 cut(s) 94, 205, 238, 573
BlsI GCNGC 4 cut(s) 95, 206, 239, 574
Bme1390I CCNGG 3 cut(s) 191, 215, 283
Bme18I GGWCC 2 cut(s) 20, 259
BmgT120I GGNCC 3 cut(s) 20, 79, 259
BmiI GGNNCC 3 cut(s) 21, 73, 81
BmrFI CCNGG 3 cut(s) 191, 215, 283
BmsI GCATC 3 cut(s) 114, 129, 183
Bpu10I CCTNAGC 1 cut(s) 330
BpuEI CTTGAG 2 cut(s) 518, 524
BpuMI CCSGG 2 cut(s) 215, 283
BsaHI GRCGYC 2 cut(s) 72, 321
BsaJI CCNNGG 2 cut(s) 75, 189
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 2 cut(s) 359, 386
Bse1I ACTGG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 2 cut(s) 75, 189
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 2 cut(s) 359, 386
BseMII CTCAG 2 cut(s) 344, 519
BseNI ACTGG 1 cut(s) 12
BseRI GAGGAG 8 cut(s) 44, 126, 152, 478, 481, 484, 487, 490
BseSI GKGCMC 1 cut(s) 381
BseX3I CGGCCG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 105, 559
BseYI CCCAGC 2 cut(s) 228, 380
Bsh1236I CGCG 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 4 cut(s) 80, 159, 300, 390
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 2 cut(s) 339, 534
BsiSI CCGG 6 cut(s) 136, 214, 282, 318, 353, 387
BslFI GGGAC 3 cut(s) 18, 298, 421
BslI CCNNNNNNNGG 2 cut(s) 359, 386
BsmAI GTCTC 3 cut(s) 283, 328, 453
BsmBI CGTCTC 2 cut(s) 283, 328
BsmFI GGGAC 3 cut(s) 18, 298, 421
BsnI GGCC 4 cut(s) 80, 159, 300, 390
Bsp1286I GDGCHC 3 cut(s) 339, 381, 534
Bsp143I GATC 4 cut(s) 66, 127, 472, 481
BspACI CCGC 5 cut(s) 204, 238, 262, 327, 452
BspANI GGCC 4 cut(s) 80, 159, 300, 390
BspCNI CTCAG 2 cut(s) 343, 520
BspFNI CGCG 1 cut(s) 240
BspLI GGNNCC 3 cut(s) 21, 73, 81
BspPI GGATC 2 cut(s) 467, 476
BspQI GCTCTTC 1 cut(s) 344
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 329
BsrI ACTGG 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 75, 189
BssMI GATC 4 cut(s) 66, 127, 472, 481
BssNI GRCGYC 2 cut(s) 72, 321
Bst2UI CCWGG 1 cut(s) 191
Bst6I CTCTTC 3 cut(s) 344, 446, 508
BstACI GRCGYC 2 cut(s) 72, 321
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 2 cut(s) 330, 528
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 240
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 242
BstKTI GATC 4 cut(s) 69, 130, 475, 484
BstMAI GTCTC 3 cut(s) 283, 328, 453
BstMBI GATC 4 cut(s) 66, 127, 472, 481
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 3 cut(s) 189, 213, 281
BstSLI GKGCMC 1 cut(s) 381
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 2 cut(s) 105, 559
BstZI CGGCCG 1 cut(s) 298
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 4 cut(s) 80, 159, 300, 390
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 1 cut(s) 379
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 149
CfoI GCGC 2 cut(s) 74, 242
Cfr13I GGNCC 3 cut(s) 20, 79, 259
CseI GACGC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 565
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 2 cut(s) 330, 528
DinI GGCGCC 1 cut(s) 73
DpnI GATC 4 cut(s) 68, 129, 474, 483
DpnII GATC 4 cut(s) 66, 127, 472, 481
EaeI YGGCCR 1 cut(s) 298
EagI CGGCCG 1 cut(s) 298
Eam1104I CTCTTC 3 cut(s) 344, 446, 508
EarI CTCTTC 3 cut(s) 344, 446, 508
EciI GGCGGA 1 cut(s) 441
Ecl136II GAGCTC 2 cut(s) 337, 532
EclXI CGGCCG 1 cut(s) 298
Eco24I GRGCYC 2 cut(s) 339, 534
Eco47I GGWCC 2 cut(s) 20, 259
Eco52I CGGCCG 1 cut(s) 298
Eco53kI GAGCTC 2 cut(s) 337, 532
EcoICRI GAGCTC 2 cut(s) 337, 532
EcoRI GAATTC 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 189
EcoT38I GRGCYC 2 cut(s) 339, 534
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
Esp3I CGTCTC 2 cut(s) 283, 328
FaiI YATR 2 cut(s) 144, 580
FaqI GGGAC 3 cut(s) 18, 298, 421
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 4 cut(s) 94, 205, 238, 573
FokI GGATG 1 cut(s) 386
FriOI GRGCYC 2 cut(s) 339, 534
Fsp4HI GCNGC 4 cut(s) 94, 205, 238, 573
GlaI GCGC 2 cut(s) 73, 241
GluI GCNGC 4 cut(s) 94, 205, 238, 573
GsaI CCCAGC 2 cut(s) 232, 384
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 4 cut(s) 80, 159, 300, 390
HapII CCGG 6 cut(s) 136, 214, 282, 318, 353, 387
HgaI GACGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 74, 242
Hin1I GRCGYC 2 cut(s) 72, 321
Hin6I GCGC 2 cut(s) 72, 240
HinP1I GCGC 2 cut(s) 72, 240
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 6 cut(s) 197, 253, 286, 303, 446, 544
HpaII CCGG 6 cut(s) 136, 214, 282, 318, 353, 387
HphI GGTGA 2 cut(s) 122, 367
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 5 cut(s) 110, 368, 481, 529, 553
Hpy188III TCNNGA 4 cut(s) 250, 332, 470, 541
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 1 cut(s) 486
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 16, 462
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 2 cut(s) 330, 528
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 2 cut(s) 72, 321
HspAI GCGC 2 cut(s) 72, 240
KasI GGCGCC 1 cut(s) 71
Kzo9I GATC 4 cut(s) 66, 127, 472, 481
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 3 cut(s) 57, 334, 389
Lsp1109I GCAGC 2 cut(s) 105, 559
LweI GCATC 3 cut(s) 114, 129, 183
MaeII ACGT 1 cut(s) 276
MalI GATC 4 cut(s) 68, 129, 474, 483
MbiI CCGCTC 1 cut(s) 329
MboI GATC 4 cut(s) 66, 127, 472, 481
MboII GAAGA 8 cut(s) 38, 331, 433, 495, 498, 501, 504, 566
MhlI GDGCHC 3 cut(s) 339, 381, 534
MluCI AATT 3 cut(s) 11, 209, 517
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 280, 455, 538
MspA1I CMGCKG 1 cut(s) 204
MspI CCGG 6 cut(s) 136, 214, 282, 318, 353, 387
MspR9I CCNGG 3 cut(s) 191, 215, 283
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 102
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 2 cut(s) 215, 283
NdeII GATC 4 cut(s) 66, 127, 472, 481
NlaIV GGNNCC 3 cut(s) 21, 73, 81
PciSI GCTCTTC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 3 cut(s) 273, 408, 414
PfeI GAWTC 3 cut(s) 197, 253, 303
PfoI TCCNGGA 1 cut(s) 281
PkrI GCNGC 4 cut(s) 95, 206, 239, 574
PleI GAGTC 3 cut(s) 280, 454, 538
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 280, 454, 538
Psp124BI GAGCTC 2 cut(s) 339, 534
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 2 cut(s) 228, 380
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 3 cut(s) 21, 73, 81
PspPI GGNCC 3 cut(s) 20, 79, 259
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
SacI GAGCTC 2 cut(s) 339, 534
SalI GTCGAC 1 cut(s) 416
SapI GCTCTTC 1 cut(s) 344
SatI GCNGC 4 cut(s) 94, 205, 238, 573
Sau3AI GATC 4 cut(s) 66, 127, 472, 481
Sau96I GGNCC 3 cut(s) 20, 79, 259
SchI GAGTC 3 cut(s) 280, 455, 538
ScrFI CCNGG 3 cut(s) 191, 215, 283
SduI GDGCHC 3 cut(s) 339, 381, 534
SetI ASST 9 cut(s) 135, 261, 279, 339, 364, 386, 534, 540, 574
SfaNI GCATC 3 cut(s) 114, 129, 183
SfoI GGCGCC 1 cut(s) 73
SinI GGWCC 2 cut(s) 20, 259
SmlI CTYRAG 2 cut(s) 533, 539
SmoI CTYRAG 2 cut(s) 533, 539
Sse9I AATT 3 cut(s) 11, 209, 517
SsiI CCGC 5 cut(s) 204, 238, 262, 327, 452
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 185
SstI GAGCTC 2 cut(s) 339, 534
StyD4I CCNGG 3 cut(s) 189, 213, 281
TaiI ACGT 1 cut(s) 279
TaqI TCGA 4 cut(s) 249, 402, 417, 444
TasI AATT 3 cut(s) 11, 209, 517
TauI GCSGC 2 cut(s) 207, 240
TfiI GAWTC 3 cut(s) 197, 253, 303
TscAI CASTG 1 cut(s) 469
TseI GCWGC 2 cut(s) 93, 572
TspDTI ATGAA 1 cut(s) 567
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 2 cut(s) 20, 259
XapI RAATTY 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.