pycom05g15040

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000944
Physical Location & Seq
Forward (+)
18579644 .. 18580878
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15040.2

Sequence Viewer

Length: 1014 bp
ATGATGACGGTGCTTTTGGTTCGTTATGGCCTCTCAAGTTACGAGGATTCTAATTTTCCAACTGTATATTTTAACTGTTTAACCAACACCTCGCTCCTCGCCTTCTCCGAAACTCTTATCCAATATCTCCACCTCCGCCTCCTCCTCTTCCTCCACCTCCTCGTGCTTATCAGGTTCCGCCACCTCCCCGTGCTTATCAGGTTCCTCCACCTCCTCGTGCTTATCAGATTCCACGACAAAAATCACCCACCAAATCCCGCCAAATTACCACAACCACCGTTTACGTACCCACATGGCAGGCCCCCATTGGGCCTGGCACCACTCTCTGCTTCTACACTTCTACTAGATCACTACCTCCACTCCAAAAATCTTCCAATTCCCTCCTCCTCCAAAATCTCAAAAACCCTAATTCTTCCAAACCTCTGTAAATATCACCACCACCACAAAAAAAAAAAAAAAAAAAAAATCCTCTCTTTTGAGTTGATTTTAACAAACATGCAGCTGCTAGAATTCATGTTGTGCTTCCTTCTCTTATGGATCACGTGCTCAAGTTCGAAGCCAACGTCGATGTACGACGTTTTGCGGGCCCACGGGCTTCCGATGGGACTGCTGCCGAAAGGGGTGGTCGACTTCGAAATCGACGACGCCGGCAGGTTTACGGTCCACCTGGATCAGGCCTGCAATGCCAAGTTCGAGAGCGAGTTGCATTACGACAGAAACGTTTCGGGGATGCTGAGTTTCGGGCAGATCGGATCCTTGTCGGGTATTTCGGCCCAGGAGCTGTTCCTCTGGTTCCCCGTTAAGGGTATCCGGGTCGACGTCCCCAGCTCCGGCATCATCTACTTCGATGTCGGGGTTGTTTATAAGCAGTTCTCGCTGTCGCTTTTTGAAACGCCACCGGAATGCACGGCTGCCTCTGGCGGCGTTGAAGCCGTTGGGGCGGTGTCCAAGAGTGAATCGGGGAGATTGCGGTCTAAGCTCCTTTTCGATCATGTTGGGAGAGATTTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

338

Amino Acids

38.03

Weight (kDa)

9.21

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AatII GACGTC 1 cut(s) 822
Acc36I ACCTGC 1 cut(s) 642
AccB1I GGYRCC 1 cut(s) 316
AccI GTMKAC 2 cut(s) 627, 816
AciI CCGC 7 cut(s) 136, 178, 258, 583, 921, 941, 970
AclI AACGTT 1 cut(s) 720
AclWI GGATC 4 cut(s) 545, 678, 747, 760
AcsI RAATTY 1 cut(s) 509
AcvI CACGTG 1 cut(s) 543
AcyI GRCGYC 2 cut(s) 645, 819
AfaI GTAC 2 cut(s) 287, 572
AfiI CCNNNNNNNGG 5 cut(s) 308, 673, 802, 803, 830
AgsI TTSAA 2 cut(s) 890, 929
AjnI CCWGG 3 cut(s) 312, 666, 774
AluBI AGCT 4 cut(s) 502, 781, 828, 979
AluI AGCT 4 cut(s) 502, 781, 828, 979
Alw21I GWGCWC 1 cut(s) 548
AlwI GGATC 4 cut(s) 545, 678, 747, 760
AlwNI CAGNNNCTG 1 cut(s) 781
AoxI GGCC 6 cut(s) 28, 299, 310, 585, 675, 771
ApaI GGGCCC 1 cut(s) 589
ApeKI GCWGC 4 cut(s) 499, 502, 610, 911
ApoI RAATTY 1 cut(s) 509
ArsI GACNNNNNNTTYG 3 cut(s) 30, 548, 580
Asp700I GAANNNNTTC 1 cut(s) 721
AspS9I GGNCC 6 cut(s) 300, 310, 585, 586, 661, 772
AsuC2I CCSGG 1 cut(s) 812
AsuHPI GGTGA 2 cut(s) 236, 425
AsuII TTCGAA 2 cut(s) 554, 633
AvaII GGWCC 1 cut(s) 661
BaeGI GKGCMC 1 cut(s) 589
BamHI GGATCC 1 cut(s) 752
BanI GGYRCC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 589
BauI CACGAG 2 cut(s) 161, 215
BbrPI CACGTG 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 548
BbvI GCAGC 4 cut(s) 489, 511, 597, 898
BccI CCATC 1 cut(s) 595
BceAI ACGGC 2 cut(s) 917, 924
BcgI CGANNNNNNTGC 2 cut(s) 589, 623
BciT130I CCWGG 3 cut(s) 314, 668, 776
BciVI GTATCC 1 cut(s) 818
BcnI CCSGG 1 cut(s) 812
BfaI CTAG 2 cut(s) 344, 506
BfuAI ACCTGC 1 cut(s) 642
BfuI GTATCC 1 cut(s) 818
BglI GCCNNNNNGGC 1 cut(s) 938
BisI GCNGC 5 cut(s) 500, 503, 611, 912, 922
BlsI GCNGC 5 cut(s) 501, 504, 612, 913, 923
Bme1390I CCNGG 4 cut(s) 314, 668, 776, 812
Bme18I GGWCC 1 cut(s) 661
BmgT120I GGNCC 6 cut(s) 300, 310, 585, 586, 661, 772
BmiI GGNNCC 7 cut(s) 176, 203, 302, 318, 587, 754, 794
BmrFI CCNGG 4 cut(s) 314, 668, 776, 812
BmsI GCATC 2 cut(s) 720, 843
Bpu14I TTCGAA 2 cut(s) 554, 633
BpuEI CTTGAG 2 cut(s) 19, 532
BpuMI CCSGG 1 cut(s) 812
BsaAI YACGTR 2 cut(s) 285, 543
BsaHI GRCGYC 2 cut(s) 645, 819
BsaJI CCNNGG 2 cut(s) 589, 774
BsaWI WCCGGW 1 cut(s) 898
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 5 cut(s) 308, 673, 802, 803, 830
Bse118I RCCGGY 1 cut(s) 647
Bse3DI GCAATG 1 cut(s) 688
BseBI CCWGG 3 cut(s) 314, 668, 776
BseDI CCNNGG 2 cut(s) 589, 774
BseGI GGATG 1 cut(s) 735
BseLI CCNNNNNNNGG 5 cut(s) 308, 673, 802, 803, 830
BseMI GCAATG 1 cut(s) 688
BseMII CTCAG 1 cut(s) 725
BseRI GAGGAG 7 cut(s) 86, 131, 134, 149, 203, 373, 376
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 4 cut(s) 489, 511, 597, 898
BseYI CCCAGC 1 cut(s) 824
BshFI GGCC 6 cut(s) 30, 301, 312, 587, 677, 773
BshNI GGYRCC 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 548
BsiSI CCGG 4 cut(s) 648, 811, 831, 899
BslFI GGGAC 2 cut(s) 618, 806
BslI CCNNNNNNNGG 5 cut(s) 308, 673, 802, 803, 830
BsmFI GGGAC 2 cut(s) 618, 806
BsmI GAATGC 1 cut(s) 908
BsnI GGCC 6 cut(s) 30, 301, 312, 587, 677, 773
Bsp119I TTCGAA 2 cut(s) 554, 633
Bsp120I GGGCCC 1 cut(s) 585
Bsp1286I GDGCHC 2 cut(s) 548, 589
Bsp143I GATC 6 cut(s) 346, 537, 670, 747, 752, 988
BspACI CCGC 7 cut(s) 136, 178, 258, 583, 921, 941, 970
BspANI GGCC 6 cut(s) 30, 301, 312, 587, 677, 773
BspCNI CTCAG 1 cut(s) 726
BspLI GGNNCC 7 cut(s) 176, 203, 302, 318, 587, 754, 794
BspMI ACCTGC 1 cut(s) 642
BspPI GGATC 4 cut(s) 545, 678, 747, 760
BspT104I TTCGAA 2 cut(s) 554, 633
BspT107I GGYRCC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 688
BsrFI RCCGGY 1 cut(s) 647
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 2 cut(s) 589, 774
BssMI GATC 6 cut(s) 346, 537, 670, 747, 752, 988
BssNI GRCGYC 2 cut(s) 645, 819
BssSI CACGAG 2 cut(s) 161, 215
Bst2BI CACGAG 2 cut(s) 161, 215
Bst2UI CCWGG 3 cut(s) 314, 668, 776
Bst4CI ACNGT 5 cut(s) 10, 64, 77, 279, 661
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 2 cut(s) 645, 819
BstBAI YACGTR 2 cut(s) 285, 543
BstBI TTCGAA 2 cut(s) 554, 633
BstC8I GCNNGC 4 cut(s) 299, 585, 649, 679
BstDEI CTNAG 2 cut(s) 734, 975
BstDSI CCRYGG 1 cut(s) 589
BstENI CCTNNNNNAGG 1 cut(s) 671
BstF5I GGATG 1 cut(s) 735
BstKTI GATC 6 cut(s) 349, 540, 673, 750, 755, 991
BstMBI GATC 6 cut(s) 346, 537, 670, 747, 752, 988
BstMWI GCNNNNNNNGC 4 cut(s) 683, 874, 938, 976
BstNI CCWGG 3 cut(s) 314, 668, 776
BstNSI RCATGY 1 cut(s) 499
BstSCI CCNGG 4 cut(s) 312, 666, 774, 810
BstSLI GKGCMC 1 cut(s) 589
BstSNI TACGTA 1 cut(s) 285
BstV1I GCAGC 4 cut(s) 489, 511, 597, 898
BstX2I RGATCY 1 cut(s) 752
BstYI RGATCY 1 cut(s) 752
BsuI GTATCC 1 cut(s) 818
BsuRI GGCC 6 cut(s) 30, 301, 312, 587, 677, 773
BtgI CCRYGG 1 cut(s) 589
BtsCI GGATG 1 cut(s) 735
BveI ACCTGC 1 cut(s) 642
Cac8I GCNNGC 4 cut(s) 299, 585, 649, 679
CaiI CAGNNNCTG 1 cut(s) 781
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 6 cut(s) 300, 310, 585, 586, 661, 772
CseI GACGC 1 cut(s) 653
Csp6I GTAC 2 cut(s) 286, 571
CviAII CATG 4 cut(s) 293, 496, 514, 992
CviQI GTAC 2 cut(s) 286, 571
DdeI CTNAG 2 cut(s) 734, 975
DpnI GATC 6 cut(s) 348, 539, 672, 749, 754, 990
DpnII GATC 6 cut(s) 346, 537, 670, 747, 752, 988
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
EciI GGCGGA 2 cut(s) 125, 167
Eco105I TACGTA 1 cut(s) 285
Eco147I AGGCCT 1 cut(s) 677
Eco24I GRGCYC 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 661
Eco72I CACGTG 1 cut(s) 543
EcoNI CCTNNNNNAGG 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 300
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 3 cut(s) 312, 666, 774
EcoT38I GRGCYC 1 cut(s) 589
FaeI CATG 4 cut(s) 296, 499, 517, 995
FaiI YATR 8 cut(s) 27, 67, 294, 497, 515, 535, 864, 993
FaqI GGGAC 2 cut(s) 618, 806
FatI CATG 4 cut(s) 292, 495, 513, 991
FauI CCCGC 2 cut(s) 265, 576
FblI GTMKAC 2 cut(s) 627, 816
Fnu4HI GCNGC 5 cut(s) 500, 503, 611, 912, 922
FokI GGATG 1 cut(s) 742
FriOI GRGCYC 1 cut(s) 589
Fsp4HI GCNGC 5 cut(s) 500, 503, 611, 912, 922
FspBI CTAG 2 cut(s) 344, 506
GluI GCNGC 5 cut(s) 500, 503, 611, 912, 922
GsaI CCCAGC 1 cut(s) 828
HaeIII GGCC 6 cut(s) 30, 301, 312, 587, 677, 773
HapII CCGG 4 cut(s) 648, 811, 831, 899
HgaI GACGC 1 cut(s) 653
Hin1I GRCGYC 2 cut(s) 645, 819
Hin1II CATG 4 cut(s) 296, 499, 517, 995
HincII GTYRAC 2 cut(s) 628, 817
HindII GTYRAC 2 cut(s) 628, 817
HinfI GANTC 3 cut(s) 47, 228, 956
HpaII CCGG 4 cut(s) 648, 811, 831, 899
HphI GGTGA 2 cut(s) 236, 425
Hpy166II GTNNAC 5 cut(s) 282, 628, 657, 664, 817
Hpy188I TCNGA 4 cut(s) 109, 227, 600, 752
Hpy188III TCNNGA 1 cut(s) 694
Hpy8I GTNNAC 5 cut(s) 282, 628, 657, 664, 817
Hpy99I CGWCG 5 cut(s) 568, 578, 644, 647, 821
HpyAV CCTTC 2 cut(s) 112, 536
HpyCH4III ACNGT 5 cut(s) 10, 64, 77, 279, 661
HpyCH4IV ACGT 6 cut(s) 284, 542, 563, 576, 720, 819
HpyCH4V TGCA 4 cut(s) 499, 681, 706, 906
HpyF10VI GCNNNNNNNGC 4 cut(s) 683, 874, 938, 976
HpyF3I CTNAG 2 cut(s) 734, 975
HpySE526I ACGT 6 cut(s) 284, 542, 563, 576, 720, 819
Hsp92I GRCGYC 2 cut(s) 645, 819
Hsp92II CATG 4 cut(s) 296, 499, 517, 995
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
Kzo9I GATC 6 cut(s) 346, 537, 670, 747, 752, 988
LmnI GCTCC 4 cut(s) 99, 778, 833, 984
Lsp1109I GCAGC 4 cut(s) 489, 511, 597, 898
LweI GCATC 2 cut(s) 720, 843
MaeI CTAG 2 cut(s) 344, 506
MaeII ACGT 6 cut(s) 284, 542, 563, 576, 720, 819
MaeIII GTNAC 1 cut(s) 38
MalI GATC 6 cut(s) 348, 539, 672, 749, 754, 990
MboI GATC 6 cut(s) 346, 537, 670, 747, 752, 988
MboII GAAGA 3 cut(s) 139, 362, 404
MflI RGATCY 1 cut(s) 752
MhlI GDGCHC 2 cut(s) 548, 589
MluCI AATT 5 cut(s) 52, 263, 375, 408, 509
MmeI TCCRAC 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 647
MroXI GAANNNNTTC 1 cut(s) 721
MseI TTAA 4 cut(s) 72, 80, 488, 801
MslI CAYNNNNRTG 1 cut(s) 901
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 4 cut(s) 648, 811, 831, 899
MspR9I CCNGG 4 cut(s) 314, 668, 776, 812
Mva1269I GAATGC 1 cut(s) 908
MvaI CCWGG 3 cut(s) 314, 668, 776
MwoI GCNNNNNNNGC 4 cut(s) 683, 874, 938, 976
NaeI GCCGGC 1 cut(s) 649
NciI CCSGG 1 cut(s) 812
NdeII GATC 6 cut(s) 346, 537, 670, 747, 752, 988
NgoMIV GCCGGC 1 cut(s) 647
NlaIII CATG 4 cut(s) 296, 499, 517, 995
NlaIV GGNNCC 7 cut(s) 176, 203, 302, 318, 587, 754, 794
NspI RCATGY 1 cut(s) 499
NspV TTCGAA 2 cut(s) 554, 633
PceI AGGCCT 1 cut(s) 677
PcsI WCGNNNNNNNCGW 4 cut(s) 105, 560, 639, 717
PctI GAATGC 1 cut(s) 908
PdiI GCCGGC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 721
PfeI GAWTC 3 cut(s) 47, 228, 956
PkrI GCNGC 5 cut(s) 501, 504, 612, 913, 923
PmaCI CACGTG 1 cut(s) 543
PmlI CACGTG 1 cut(s) 543
Ppu21I YACGTR 2 cut(s) 285, 543
PsiI TTATAA 1 cut(s) 864
Psp1406I AACGTT 1 cut(s) 720
Psp6I CCWGG 3 cut(s) 312, 666, 774
PspCI CACGTG 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 3 cut(s) 312, 666, 774
PspN4I GGNNCC 7 cut(s) 176, 203, 302, 318, 587, 754, 794
PspOMI GGGCCC 1 cut(s) 585
PspPI GGNCC 6 cut(s) 300, 310, 585, 586, 661, 772
PstNI CAGNNNCTG 1 cut(s) 781
PsuI RGATCY 1 cut(s) 752
PvuII CAGCTG 1 cut(s) 502
RsaI GTAC 2 cut(s) 287, 572
RsaNI GTAC 2 cut(s) 286, 571
RseI CAYNNNNRTG 1 cut(s) 901
SalI GTCGAC 2 cut(s) 626, 815
SaqAI TTAA 4 cut(s) 72, 80, 488, 801
SatI GCNGC 5 cut(s) 500, 503, 611, 912, 922
Sau3AI GATC 6 cut(s) 346, 537, 670, 747, 752, 988
Sau96I GGNCC 6 cut(s) 300, 310, 585, 586, 661, 772
ScrFI CCNGG 4 cut(s) 314, 668, 776, 812
SduI GDGCHC 2 cut(s) 548, 589
SfaNI GCATC 2 cut(s) 720, 843
SfuI TTCGAA 2 cut(s) 554, 633
SinI GGWCC 1 cut(s) 661
SmiMI CAYNNNNRTG 1 cut(s) 901
SmlI CTYRAG 2 cut(s) 34, 547
SmoI CTYRAG 2 cut(s) 34, 547
SnaBI TACGTA 1 cut(s) 285
Sse9I AATT 5 cut(s) 52, 263, 375, 408, 509
SseBI AGGCCT 1 cut(s) 677
SsiI CCGC 7 cut(s) 136, 178, 258, 583, 921, 941, 970
SspMI CTAG 2 cut(s) 344, 506
StuI AGGCCT 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 312, 666, 774, 810
TaaI ACNGT 5 cut(s) 10, 64, 77, 279, 661
TaiI ACGT 6 cut(s) 287, 545, 566, 579, 723, 822
TaqI TCGA 9 cut(s) 554, 566, 627, 633, 639, 693, 816, 846, 987
TasI AATT 5 cut(s) 52, 263, 375, 408, 509
TauI GCSGC 1 cut(s) 924
TfiI GAWTC 3 cut(s) 47, 228, 956
Tru1I TTAA 4 cut(s) 72, 80, 488, 801
Tru9I TTAA 4 cut(s) 72, 80, 488, 801
TseI GCWGC 4 cut(s) 499, 502, 610, 911
TspDTI ATGAA 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 661
XagI CCTNNNNNAGG 1 cut(s) 671
XapI RAATTY 1 cut(s) 509
XceI RCATGY 1 cut(s) 499
XmiI GTMKAC 2 cut(s) 627, 816
XmnI GAANNNNTTC 1 cut(s) 721
XspI CTAG 2 cut(s) 344, 506
ZraI GACGTC 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.