FvH4_2g17710

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000944
Physical Location & Seq
Forward (+)
15227817 .. 15228409
593 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g17710.t1

Sequence Viewer

Length: 498 bp
ATGACCATCACCTCCGATTCAAAGCCGCCCCCGTCGGTCTACGAAGTCCTCCGCGCACATGGTCTTCCGATGGGGCTGCTCCCCAAGGGAGTGACCAAGTTCGAAATCGACGAGGGCGGCAGGTTCCAGGTCCACCTGGATCAGGCTTGCAATGCCAAGTTCGAGAGTGAATTGCATTACGACCGGAACGTGTCGGGTACTCTCAGCTACGGCCGGATCGGAGCTTTGTCGGGTATTTCGGCGCAGGAGCTTTTCCTCTGGTTTCCGGTGAAGGGTATCCGGGTGGATGTGCCCAGCTCCGGTATCATTTACTTCGATGTAGGCGTTGTTTATAAGCAATTCTCGCTTTCGCTATTCGAAACGCCGCCGGATTGTACTGCCGCCACCGCCGATGTCGATGGCGGCAGGCTCATCGTTGACGCCGTTTCCAAGAGTAATTCGGGAAAACTGCGGTATGGTCTTGATCAAGAGCAACATTTTGGGAGAGGTTTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

166

Amino Acids

18.0

Weight (kDa)

5.77

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 13 - 120 1.6e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
Acc36I ACCTGC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 39
AccII CGCG 1 cut(s) 54
AciI CCGC 8 cut(s) 26, 52, 117, 365, 381, 387, 402, 451
AclWI GGATC 2 cut(s) 147, 224
AcoI YGGCCR 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 2 cut(s) 199, 376
AfiI CCNNNNNNNGG 3 cut(s) 142, 272, 299
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 1 cut(s) 21
AjnI CCWGG 2 cut(s) 126, 135
AluBI AGCT 4 cut(s) 207, 224, 250, 297
AluI AGCT 4 cut(s) 207, 224, 250, 297
AlwI GGATC 2 cut(s) 147, 224
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 76
AspLEI GCGC 2 cut(s) 56, 244
AspS9I GGNCC 1 cut(s) 130
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 280
AsuII TTCGAA 2 cut(s) 102, 357
AvaII GGWCC 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 63
BccI CCATC 3 cut(s) 14, 64, 392
BceAI ACGGC 2 cut(s) 226, 407
BcgI CGANNNNNNTGC 4 cut(s) 58, 92, 394, 428
BciT130I CCWGG 2 cut(s) 128, 137
BciVI GTATCC 1 cut(s) 287
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 1 cut(s) 281
BfuAI ACCTGC 1 cut(s) 111
BfuI GTATCC 1 cut(s) 287
BisI GCNGC 6 cut(s) 26, 77, 118, 365, 381, 403
BlsI GCNGC 6 cut(s) 27, 78, 119, 366, 382, 404
Bme1390I CCNGG 3 cut(s) 128, 137, 281
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 125
BmrFI CCNGG 3 cut(s) 128, 137, 281
BpiI GAAGAC 1 cut(s) 56
Bpu14I TTCGAA 2 cut(s) 102, 357
BpuMI CCSGG 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 1 cut(s) 84
BsaWI WCCGGW 3 cut(s) 183, 265, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 272, 299
Bse3DI GCAATG 1 cut(s) 157
BseBI CCWGG 2 cut(s) 128, 137
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 3 cut(s) 142, 272, 299
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 217
BseSI GKGCMC 1 cut(s) 294
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 2 cut(s) 184, 214
BshFI GGCC 1 cut(s) 213
BsiEI CGRYCG 2 cut(s) 184, 214
BsiSI CCGG 6 cut(s) 184, 214, 266, 280, 300, 368
BslI CCNNNNNNNGG 3 cut(s) 142, 272, 299
BsnI GGCC 1 cut(s) 213
Bsp119I TTCGAA 2 cut(s) 102, 357
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 3 cut(s) 139, 216, 463
BspACI CCGC 8 cut(s) 26, 52, 117, 365, 381, 387, 402, 451
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 1 cut(s) 216
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 125
BspMI ACCTGC 1 cut(s) 111
BspPI GGATC 2 cut(s) 147, 224
BspT104I TTCGAA 2 cut(s) 102, 357
BsrDI GCAATG 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 3 cut(s) 139, 216, 463
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 128, 137
BstACI GRCGYC 1 cut(s) 420
BstBI TTCGAA 2 cut(s) 102, 357
BstC8I GCNNGC 2 cut(s) 148, 407
BstDEI CTNAG 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 54
BstHHI GCGC 2 cut(s) 56, 244
BstKTI GATC 3 cut(s) 142, 219, 466
BstMBI GATC 3 cut(s) 139, 216, 463
BstMCI CGRYCG 2 cut(s) 184, 214
BstMWI GCNNNNNNNGC 3 cut(s) 152, 343, 386
BstNI CCWGG 2 cut(s) 128, 137
BstSCI CCNGG 3 cut(s) 126, 135, 279
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 63
BstV2I GAAGAC 1 cut(s) 56
BstZI CGGCCG 1 cut(s) 211
BsuI GTATCC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 213
BtsCI GGATG 1 cut(s) 292
BveI ACCTGC 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 148, 407
CfoI GCGC 2 cut(s) 56, 244
Cfr13I GGNCC 1 cut(s) 130
CseI GACGC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 198, 375
CviAII CATG 1 cut(s) 59
CviJI RGCY 9 cut(s) 25, 76, 146, 207, 213, 224, 250, 297, 409
CviKI_1 RGCY 9 cut(s) 25, 76, 146, 207, 213, 224, 250, 297, 409
CviQI GTAC 2 cut(s) 198, 375
DdeI CTNAG 1 cut(s) 203
DpnI GATC 3 cut(s) 141, 218, 465
DpnII GATC 3 cut(s) 139, 216, 463
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 130
Eco52I CGGCCG 1 cut(s) 211
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 126, 135
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 1 cut(s) 62
FaiI YATR 3 cut(s) 60, 333, 456
FatI CATG 1 cut(s) 58
FbaI TGATCA 1 cut(s) 463
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 6 cut(s) 26, 77, 118, 365, 381, 403
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 6 cut(s) 26, 77, 118, 365, 381, 403
GlaI GCGC 2 cut(s) 55, 243
GluI GCNGC 6 cut(s) 26, 77, 118, 365, 381, 403
GsaI CCCAGC 1 cut(s) 297
HaeIII GGCC 1 cut(s) 213
HapII CCGG 6 cut(s) 184, 214, 266, 280, 300, 368
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 56, 244
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 1 cut(s) 62
Hin6I GCGC 2 cut(s) 54, 242
HinP1I GCGC 2 cut(s) 54, 242
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 1 cut(s) 17
HpaII CCGG 6 cut(s) 184, 214, 266, 280, 300, 368
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 3 cut(s) 40, 133, 418
Hpy188I TCNGA 3 cut(s) 16, 69, 221
Hpy188III TCNNGA 4 cut(s) 163, 441, 461, 467
Hpy8I GTNNAC 3 cut(s) 40, 133, 418
Hpy99I CGWCG 2 cut(s) 37, 113
HpyAV CCTTC 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 150, 175
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 343, 386
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 1 cut(s) 62
HspAI GCGC 2 cut(s) 54, 242
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 3 cut(s) 139, 216, 463
LmnI GCTCC 4 cut(s) 84, 221, 247, 302
Lsp1109I GCAGC 1 cut(s) 63
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 91
MalI GATC 3 cut(s) 141, 218, 465
MboI GATC 3 cut(s) 139, 216, 463
MboII GAAGA 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 3 cut(s) 170, 338, 436
MnlI CCTC 5 cut(s) 22, 59, 106, 266, 479
MspI CCGG 6 cut(s) 184, 214, 266, 280, 300, 368
MspR9I CCNGG 3 cut(s) 128, 137, 281
MvaI CCWGG 2 cut(s) 128, 137
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 3 cut(s) 152, 343, 386
NciI CCSGG 1 cut(s) 281
NdeII GATC 3 cut(s) 139, 216, 463
NlaIII CATG 1 cut(s) 62
NlaIV GGNNCC 1 cut(s) 125
NmuCI GTSAC 1 cut(s) 91
NspV TTCGAA 2 cut(s) 102, 357
PcsI WCGNNNNNNNCGW 4 cut(s) 108, 186, 321, 420
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 6 cut(s) 27, 78, 119, 366, 382, 404
PsiI TTATAA 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 126, 135
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 2 cut(s) 126, 135
PspN4I GGNNCC 1 cut(s) 125
PspPI GGNCC 1 cut(s) 130
RsaI GTAC 2 cut(s) 199, 376
RsaNI GTAC 2 cut(s) 198, 375
SatI GCNGC 6 cut(s) 26, 77, 118, 365, 381, 403
Sau3AI GATC 3 cut(s) 139, 216, 463
Sau96I GGNCC 1 cut(s) 130
ScrFI CCNGG 3 cut(s) 128, 137, 281
SduI GDGCHC 1 cut(s) 294
SfuI TTCGAA 2 cut(s) 102, 357
SinI GGWCC 1 cut(s) 130
Sse9I AATT 3 cut(s) 170, 338, 436
SsiI CCGC 8 cut(s) 26, 52, 117, 365, 381, 387, 402, 451
StyD4I CCNGG 3 cut(s) 126, 135, 279
StyI CCWWGG 1 cut(s) 84
TaiI ACGT 1 cut(s) 192
TaqI TCGA 6 cut(s) 102, 108, 162, 315, 357, 396
TaqII GACCGA 1 cut(s) 25
TasI AATT 3 cut(s) 170, 338, 436
TatI WGTACW 1 cut(s) 374
TauI GCSGC 5 cut(s) 28, 120, 367, 383, 405
TfiI GAWTC 1 cut(s) 17
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.