Rorug06G0148700

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000944
Physical Location & Seq
Forward (+)
21273515 .. 21274090
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0148700.1

Sequence Viewer

Length: 447 bp
ATGGAGTATATGGCAGCCTCGAGAACACCTCAACTTGCTCCAGATACAGCTCCGAGCATGATCTCTCCTCCAGGTTGGACTCCTTCACCTCCGAATTATGTGAAGGTGAACTCCGACGCTGCTTGGTTGGCCCTCAATTCTACTGGCCTAGGAGTCATAATCCAGGATGTGTCTGGACTCAAAAGAACTCATCTCAGAGATAAAGAAGCCACGAAGAAAGAGGTGTTGGAGACTTATCCGGTCATTGATCAAATCCCACGGAGATGTTCTTTCTTTGAAGATTGTTGTTGGGACTGGGTTTCCCATGAAGCAAATCATGTTGTCCACTCTATAGCCTCGCTTGCCATCCGGACGGTGGGTCCACTGCGATGGGCTAATCAGCAGCCACCATCCTTGACTCTGGTATTGAGGAGCGACGGGCTTCCATGTCCTCCAGTGGTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

148

Amino Acids

16.36

Weight (kDa)

6.06

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 348
AfiI CCNNNNNNNGG 1 cut(s) 355
AgsI TTSAA 1 cut(s) 278
AhdI GACNNNNNGTC 1 cut(s) 357
AjnI CCWGG 2 cut(s) 70, 162
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 348
AoxI GGCC 2 cut(s) 129, 145
ApeKI GCWGC 3 cut(s) 14, 119, 382
AspA2I CCTAGG 1 cut(s) 148
AspS9I GGNCC 2 cut(s) 130, 359
AsuHPI GGTGA 2 cut(s) 78, 118
AvaI CYCGRG 1 cut(s) 19
AvaII GGWCC 1 cut(s) 359
AvrII CCTAGG 1 cut(s) 148
BbvI GCAGC 3 cut(s) 26, 106, 394
BccI CCATC 3 cut(s) 353, 363, 397
BciT130I CCWGG 2 cut(s) 72, 164
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 3 cut(s) 15, 120, 383
BlnI CCTAGG 1 cut(s) 148
BlsI GCNGC 3 cut(s) 16, 121, 384
Bme1390I CCNGG 2 cut(s) 72, 164
Bme18I GGWCC 1 cut(s) 359
BmeRI GACNNNNNGTC 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 130, 359
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 2 cut(s) 72, 164
BmrI ACTGGG 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 304
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BpmI CTGGAG 3 cut(s) 24, 54, 417
BsaJI CCNNGG 2 cut(s) 148, 257
BsaWI WCCGGW 2 cut(s) 238, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse1I ACTGG 3 cut(s) 148, 299, 434
BseAI TCCGGA 1 cut(s) 348
BseBI CCWGG 2 cut(s) 72, 164
BseDI CCNNGG 2 cut(s) 148, 257
BseGI GGATG 3 cut(s) 172, 345, 389
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 208
BseNI ACTGG 3 cut(s) 148, 299, 434
BseRI GAGGAG 2 cut(s) 57, 424
BseXI GCAGC 3 cut(s) 26, 106, 394
BshFI GGCC 2 cut(s) 131, 147
BsiHKCI CYCGRG 1 cut(s) 19
BsiSI CCGG 2 cut(s) 239, 349
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 131, 147
BsoBI CYCGRG 1 cut(s) 19
Bsp13I TCCGGA 1 cut(s) 348
Bsp143I GATC 2 cut(s) 60, 247
BspANI GGCC 2 cut(s) 131, 147
BspCNI CTCAG 1 cut(s) 207
BspEI TCCGGA 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 360
BsrI ACTGG 3 cut(s) 148, 299, 434
BssECI CCNNGG 2 cut(s) 148, 257
BssMI GATC 2 cut(s) 60, 247
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 72, 164
Bst4CI ACNGT 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 1 cut(s) 257
BstF5I GGATG 3 cut(s) 172, 345, 389
BstKTI GATC 2 cut(s) 63, 250
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 2 cut(s) 60, 247
BstMWI GCNNNNNNNGC 2 cut(s) 128, 341
BstNI CCWGG 2 cut(s) 72, 164
BstSCI CCNGG 2 cut(s) 70, 162
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 26, 106, 394
BstXI CCANNNNNNTGG 1 cut(s) 369
BsuRI GGCC 2 cut(s) 131, 147
BtgI CCRYGG 1 cut(s) 257
BtgZI GCGATG 1 cut(s) 382
BtsCI GGATG 3 cut(s) 172, 345, 389
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 2 cut(s) 362, 441
Cac8I GCNNGC 1 cut(s) 342
Cfr13I GGNCC 2 cut(s) 130, 359
CseI GACGC 1 cut(s) 125
CspCI CAANNNNNGTGG 2 cut(s) 375, 410
CviAII CATG 4 cut(s) 58, 305, 317, 426
CviJI RGCY 9 cut(s) 17, 50, 131, 147, 209, 335, 374, 385, 421
CviKI_1 RGCY 9 cut(s) 17, 50, 131, 147, 209, 335, 374, 385, 421
DdeI CTNAG 1 cut(s) 194
DpnI GATC 2 cut(s) 62, 249
DpnII GATC 2 cut(s) 60, 247
DriI GACNNNNNGTC 1 cut(s) 357
Eam1105I GACNNNNNGTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 359
Eco88I CYCGRG 1 cut(s) 19
EcoRII CCWGG 2 cut(s) 70, 162
EcoT14I CCWWGG 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 4 cut(s) 61, 308, 320, 429
FaiI YATR 9 cut(s) 9, 11, 59, 99, 158, 306, 318, 332, 427
FaqI GGGAC 1 cut(s) 305
FatI CATG 4 cut(s) 57, 304, 316, 425
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 3 cut(s) 15, 120, 383
FokI GGATG 3 cut(s) 179, 332, 376
Fsp4HI GCNGC 3 cut(s) 15, 120, 383
FspBI CTAG 1 cut(s) 149
GluI GCNGC 3 cut(s) 15, 120, 383
GsuI CTGGAG 3 cut(s) 24, 54, 417
HaeIII GGCC 2 cut(s) 131, 147
HapII CCGG 2 cut(s) 239, 349
HgaI GACGC 1 cut(s) 125
Hin1II CATG 4 cut(s) 61, 308, 320, 429
HinfI GANTC 4 cut(s) 79, 153, 177, 397
HpaII CCGG 2 cut(s) 239, 349
HphI GGTGA 2 cut(s) 78, 118
Hpy166II GTNNAC 3 cut(s) 109, 325, 362
Hpy188I TCNGA 4 cut(s) 54, 93, 115, 197
Hpy188III TCNNGA 4 cut(s) 21, 41, 174, 349
Hpy8I GTNNAC 3 cut(s) 109, 325, 362
Hpy99I CGWCG 2 cut(s) 119, 419
HpyAV CCTTC 2 cut(s) 93, 97
HpyCH4III ACNGT 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 341
HpyF3I CTNAG 1 cut(s) 194
Hsp92II CATG 4 cut(s) 61, 308, 320, 429
Kpn2I TCCGGA 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 60, 247
LmnI GCTCC 3 cut(s) 43, 55, 411
Lsp1109I GCAGC 3 cut(s) 26, 106, 394
MaeI CTAG 1 cut(s) 149
MalI GATC 2 cut(s) 62, 249
MboI GATC 2 cut(s) 60, 247
MboII GAAGA 2 cut(s) 226, 290
MluCI AATT 2 cut(s) 94, 136
MlyI GAGTC 4 cut(s) 73, 162, 171, 391
MmeI TCCRAC 3 cut(s) 56, 138, 207
MnlI CCTC 9 cut(s) 28, 39, 78, 99, 143, 214, 346, 402, 441
MroI TCCGGA 1 cut(s) 348
MslI CAYNNNNRTG 2 cut(s) 262, 367
MspI CCGG 2 cut(s) 239, 349
MspR9I CCNGG 2 cut(s) 72, 164
MvaI CCWGG 2 cut(s) 72, 164
MwoI GCNNNNNNNGC 2 cut(s) 128, 341
NdeII GATC 2 cut(s) 60, 247
NlaIII CATG 4 cut(s) 61, 308, 320, 429
NlaIV GGNNCC 1 cut(s) 360
PaeR7I CTCGAG 1 cut(s) 19
PfoI TCCNGGA 1 cut(s) 162
PkrI GCNGC 3 cut(s) 16, 121, 384
PleI GAGTC 4 cut(s) 73, 161, 171, 391
PpsI GAGTC 4 cut(s) 73, 161, 171, 391
Psp6I CCWGG 2 cut(s) 70, 162
PspGI CCWGG 2 cut(s) 70, 162
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 2 cut(s) 130, 359
RseI CAYNNNNRTG 2 cut(s) 262, 367
SatI GCNGC 3 cut(s) 15, 120, 383
Sau3AI GATC 2 cut(s) 60, 247
Sau96I GGNCC 2 cut(s) 130, 359
SchI GAGTC 4 cut(s) 73, 162, 171, 391
ScrFI CCNGG 2 cut(s) 72, 164
SetI ASST 6 cut(s) 31, 52, 76, 91, 108, 225
SfcI CTRYAG 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 19
SinI GGWCC 1 cut(s) 359
SlaI CTCGAG 1 cut(s) 19
SmiMI CAYNNNNRTG 2 cut(s) 262, 367
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
Sse9I AATT 2 cut(s) 94, 136
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 70, 162
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 20
TasI AATT 2 cut(s) 94, 136
TscAI CASTG 2 cut(s) 369, 441
TseI GCWGC 3 cut(s) 14, 119, 382
TspDTI ATGAA 1 cut(s) 321
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 2 cut(s) 369, 441
VpaK11BI GGWCC 1 cut(s) 359
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 352
XhoI CTCGAG 1 cut(s) 19
XmaJI CCTAGG 1 cut(s) 148
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.