RLG00000012873

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000944
Physical Location & Seq
Reverse (-)
23388651 .. 23389325
675 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012873

Sequence Viewer

Length: 576 bp
ATGTTGCTACTACAGTTCACTTCCAACTCCATACCCTTTCTCTGCTTCCTCTTATTGCTGCCGATCATGTCCATTAGCTCCGATTCAAAGCCACCCCGGTCGGTCTACGACGTCCTCCTCGCCCACGGCCTTCCGATGGGGCTGCTGCCCAAGGGTGTGACCAAGTTCGAAATCGACGAGGGCGGCCGGTTCCAGGTGTACCTGGATCAGGCCTGCAATGCCAAGTTCGAGAGTGAATTGCATTACGACAGGAACGTCTCTGGGACTCTCAGTTACGGCCAGATCGGAGCGTTGTCGGGTATTTCGGCCCAGGAGCTCTTTCTCTGGTTTCCGGTCAAGGGTATTCGGGTCGATGTCCCCAGCTCCGGCATCATTTACTTCGATGTCGGCGTTGTTTTTAAGCAATTCTCGCTTTCGCTATTCGAAACACCGCCGGATTGTACTGCTGCCTCCCCCGCCGACCAGGCTGATGTCGACGGCGGCAGGCTCATCGTTGACGCCGTTTCAAAGAGTAAATCGGGAAAACTGCGGTATGGTCTCGATCAGGAGCAACATTTTGGGAGAGGTTTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

192

Amino Acids

20.92

Weight (kDa)

5.18

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 35 - 142 3.1e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
AatII GACGTC 1 cut(s) 114
AccI GTMKAC 2 cut(s) 105, 474
AciI CCGC 5 cut(s) 183, 431, 456, 480, 529
AclWI GGATC 1 cut(s) 213
AcoI YGGCCR 2 cut(s) 184, 277
AcyI GRCGYC 2 cut(s) 111, 498
AfaI GTAC 2 cut(s) 200, 442
AfiI CCNNNNNNNGG 5 cut(s) 136, 193, 208, 338, 365
AgsI TTSAA 2 cut(s) 87, 507
AjnI CCWGG 4 cut(s) 192, 201, 309, 462
AluBI AGCT 3 cut(s) 78, 316, 363
AluI AGCT 3 cut(s) 78, 316, 363
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 262, 542
AlwI GGATC 1 cut(s) 213
AoxI GGCC 5 cut(s) 127, 184, 210, 277, 306
ApeKI GCWGC 4 cut(s) 58, 142, 145, 446
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 97
AsuII TTCGAA 2 cut(s) 168, 423
BanII GRGCYC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 318
BbvI GCAGC 4 cut(s) 45, 129, 132, 433
BccI CCATC 1 cut(s) 130
BceAI ACGGC 4 cut(s) 142, 292, 485, 493
BcgI CGANNNNNNTGC 4 cut(s) 124, 158, 472, 506
BciT130I CCWGG 4 cut(s) 194, 203, 311, 464
BcnI CCSGG 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 262, 542
BfmI CTRYAG 1 cut(s) 11
BglI GCCNNNNNGGC 1 cut(s) 464
BisI GCNGC 6 cut(s) 59, 143, 146, 184, 447, 481
BlsI GCNGC 6 cut(s) 60, 144, 147, 185, 448, 482
Bme1390I CCNGG 5 cut(s) 97, 194, 203, 311, 464
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 191
BmrFI CCNGG 5 cut(s) 97, 194, 203, 311, 464
BmsI GCATC 1 cut(s) 378
Bpu14I TTCGAA 2 cut(s) 168, 423
BpuMI CCSGG 1 cut(s) 97
BsaHI GRCGYC 2 cut(s) 111, 498
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 4 cut(s) 95, 124, 150, 309
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 193, 208, 338, 365
Bse118I RCCGGY 1 cut(s) 186
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 4 cut(s) 194, 203, 311, 464
BseDI CCNNGG 4 cut(s) 95, 124, 150, 309
BseLI CCNNNNNNNGG 5 cut(s) 136, 193, 208, 338, 365
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 107
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 4 cut(s) 45, 129, 132, 433
BseYI CCCAGC 1 cut(s) 359
Bsh1285I CGRYCG 2 cut(s) 101, 187
BshFI GGCC 5 cut(s) 129, 186, 212, 279, 308
BsiEI CGRYCG 2 cut(s) 101, 187
BsiHKAI GWGCWC 1 cut(s) 318
BsiSI CCGG 5 cut(s) 97, 187, 332, 366, 434
BslFI GGGAC 2 cut(s) 277, 341
BslI CCNNNNNNNGG 5 cut(s) 136, 193, 208, 338, 365
BsmAI GTCTC 2 cut(s) 262, 542
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 277, 341
BsnI GGCC 5 cut(s) 129, 186, 212, 279, 308
Bso31I GGTCTC 1 cut(s) 542
Bsp119I TTCGAA 2 cut(s) 168, 423
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 4 cut(s) 63, 205, 282, 541
BspACI CCGC 5 cut(s) 183, 431, 456, 480, 529
BspANI GGCC 5 cut(s) 129, 186, 212, 279, 308
BspCNI CTCAG 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 191
BspPI GGATC 1 cut(s) 213
BspT104I TTCGAA 2 cut(s) 168, 423
BspTNI GGTCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 4 cut(s) 95, 124, 150, 309
BssMI GATC 4 cut(s) 63, 205, 282, 541
BssNI GRCGYC 2 cut(s) 111, 498
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 4 cut(s) 194, 203, 311, 464
Bst4CI ACNGT 1 cut(s) 15
BstACI GRCGYC 2 cut(s) 111, 498
BstBI TTCGAA 2 cut(s) 168, 423
BstC8I GCNNGC 2 cut(s) 214, 485
BstDEI CTNAG 1 cut(s) 269
BstDSI CCRYGG 1 cut(s) 124
BstENI CCTNNNNNAGG 1 cut(s) 206
BstKTI GATC 4 cut(s) 66, 208, 285, 544
BstMAI GTCTC 2 cut(s) 262, 542
BstMBI GATC 4 cut(s) 63, 205, 282, 541
BstMCI CGRYCG 2 cut(s) 101, 187
BstMWI GCNNNNNNNGC 4 cut(s) 218, 409, 455, 464
BstNI CCWGG 4 cut(s) 194, 203, 311, 464
BstSCI CCNGG 5 cut(s) 95, 192, 201, 309, 462
BstSFI CTRYAG 1 cut(s) 11
BstV1I GCAGC 4 cut(s) 45, 129, 132, 433
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 5 cut(s) 129, 186, 212, 279, 308
BtgI CCRYGG 1 cut(s) 124
Cac8I GCNNGC 2 cut(s) 214, 485
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 307
CseI GACGC 1 cut(s) 506
Csp6I GTAC 2 cut(s) 199, 441
CviAII CATG 1 cut(s) 67
CviQI GTAC 2 cut(s) 199, 441
DdeI CTNAG 1 cut(s) 269
DpnI GATC 4 cut(s) 65, 207, 284, 543
DpnII GATC 4 cut(s) 63, 205, 282, 541
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 2 cut(s) 184, 277
EagI CGGCCG 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 316
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 212
Eco24I GRGCYC 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 542
Eco52I CGGCCG 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoRII CCWGG 4 cut(s) 192, 201, 309, 462
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 1 cut(s) 70
FaiI YATR 3 cut(s) 32, 68, 534
FaqI GGGAC 2 cut(s) 277, 341
FatI CATG 1 cut(s) 66
FauI CCCGC 1 cut(s) 463
FblI GTMKAC 2 cut(s) 105, 474
Fnu4HI GCNGC 6 cut(s) 59, 143, 146, 184, 447, 481
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 6 cut(s) 59, 143, 146, 184, 447, 481
GluI GCNGC 6 cut(s) 59, 143, 146, 184, 447, 481
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 5 cut(s) 129, 186, 212, 279, 308
HapII CCGG 5 cut(s) 97, 187, 332, 366, 434
HgaI GACGC 1 cut(s) 506
Hin1I GRCGYC 2 cut(s) 111, 498
Hin1II CATG 1 cut(s) 70
HincII GTYRAC 2 cut(s) 475, 496
HindII GTYRAC 2 cut(s) 475, 496
HinfI GANTC 2 cut(s) 83, 265
HpaII CCGG 5 cut(s) 97, 187, 332, 366, 434
Hpy166II GTNNAC 5 cut(s) 18, 106, 199, 475, 496
Hpy188I TCNGA 3 cut(s) 82, 135, 287
Hpy188III TCNNGA 4 cut(s) 229, 519, 539, 545
Hpy8I GTNNAC 5 cut(s) 18, 106, 199, 475, 496
Hpy99I CGWCG 3 cut(s) 113, 179, 479
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4IV ACGT 2 cut(s) 111, 255
HpyCH4V TGCA 2 cut(s) 216, 241
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 409, 455, 464
HpyF3I CTNAG 1 cut(s) 269
HpySE526I ACGT 2 cut(s) 111, 255
Hsp92I GRCGYC 2 cut(s) 111, 498
Hsp92II CATG 1 cut(s) 70
Kzo9I GATC 4 cut(s) 63, 205, 282, 541
LmnI GCTCC 5 cut(s) 83, 287, 313, 368, 547
Lsp1109I GCAGC 4 cut(s) 45, 129, 132, 433
LweI GCATC 1 cut(s) 378
MaeII ACGT 2 cut(s) 111, 255
MaeIII GTNAC 2 cut(s) 157, 272
MalI GATC 4 cut(s) 65, 207, 284, 543
MboI GATC 4 cut(s) 63, 205, 282, 541
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 2 cut(s) 236, 404
MlyI GAGTC 1 cut(s) 259
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 6 cut(s) 59, 125, 128, 172, 460, 557
MseI TTAA 1 cut(s) 399
MspI CCGG 5 cut(s) 97, 187, 332, 366, 434
MspR9I CCNGG 5 cut(s) 97, 194, 203, 311, 464
MvaI CCWGG 4 cut(s) 194, 203, 311, 464
MwoI GCNNNNNNNGC 4 cut(s) 218, 409, 455, 464
NciI CCSGG 1 cut(s) 97
NdeII GATC 4 cut(s) 63, 205, 282, 541
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 191
NmuCI GTSAC 1 cut(s) 157
NspV TTCGAA 2 cut(s) 168, 423
PceI AGGCCT 1 cut(s) 212
PcsI WCGNNNNNNNCGW 4 cut(s) 174, 252, 387, 498
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 6 cut(s) 60, 144, 147, 185, 448, 482
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 4 cut(s) 192, 201, 309, 462
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 4 cut(s) 192, 201, 309, 462
PspN4I GGNNCC 1 cut(s) 191
PspPI GGNCC 1 cut(s) 307
RsaI GTAC 2 cut(s) 200, 442
RsaNI GTAC 2 cut(s) 199, 441
SacI GAGCTC 1 cut(s) 318
SalI GTCGAC 1 cut(s) 473
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 6 cut(s) 59, 143, 146, 184, 447, 481
Sau3AI GATC 4 cut(s) 63, 205, 282, 541
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 5 cut(s) 97, 194, 203, 311, 464
SduI GDGCHC 1 cut(s) 318
SetI ASST 8 cut(s) 80, 114, 198, 204, 258, 318, 365, 568
SfaNI GCATC 1 cut(s) 378
SfcI CTRYAG 1 cut(s) 11
SfuI TTCGAA 2 cut(s) 168, 423
Sse9I AATT 2 cut(s) 236, 404
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 5 cut(s) 183, 431, 456, 480, 529
SstI GAGCTC 1 cut(s) 318
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 5 cut(s) 95, 192, 201, 309, 462
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 15
TaiI ACGT 2 cut(s) 114, 258
TaqI TCGA 8 cut(s) 168, 174, 228, 351, 381, 423, 474, 540
TaqII GACCGA 1 cut(s) 91
TasI AATT 2 cut(s) 236, 404
TatI WGTACW 1 cut(s) 440
TauI GCSGC 2 cut(s) 186, 483
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 4 cut(s) 58, 142, 145, 446
Tsp45I GTSAC 1 cut(s) 157
XagI CCTNNNNNAGG 1 cut(s) 206
XmiI GTMKAC 2 cut(s) 105, 474
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.