Rroxscaffold_6G00425850

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000944
Physical Location & Seq
Reverse (-)
46809741 .. 46811379
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00425850.1

Sequence Viewer

Length: 594 bp
ATGGGACTGGGATTGCAGGGTCCAAAATCCCTTGTGGGCTTTGTTCTTCTGCTCCTCCTTGCTGTGATCGGCGCCGTGGCCGGAGCATCGCCGGCCCCCAAATCCAGCAGCAAGACAAGCATCGGAGGAGGAGGAGGAGGAAGCATCTACGATCACCTCCGGCAATATGGCTTGCCCATCGGCCTCCTCCCCAAAGGCATCACGGAATATTCCCTGGACGATTCCAGCGGCGAATTCCGGGTGTTTCTGCCCCAGCCTTGCCGCGCCAAGTTCGAGAATCAGGTCCGCTACGATTTCAACGTCTCCGGGACTCTCACTTTCGGCCGAATCGCCGACTTGTCCGGCGTCTCCGCTCAGGAGCTCTTCCTTTGGTTTCCGGTGAAAGGTATCCGAGTGGATGTGCCCAGCTCCGGCCTCATTTACTTCGATGTCGGCGTTGTCGATAAGCAGTTCTCTTTGTCCCTCTTCGAGTCTCCGCCCGATTGCACTGCTCTTGATCCTTCTGATCCCTCCTCTTCTTCTTCTTCCAATTCCCCATCTGAGCTCAAGCTCAAGACTCTATCAGAAGATGTTGGGTACAAAGCTGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

197

Amino Acids

20.7

Weight (kDa)

5.13

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 49 - 157 2.8e-34 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccBSI CCGCTC 1 cut(s) 353
AccII CGCG 1 cut(s) 264
AciI CCGC 5 cut(s) 228, 262, 286, 351, 476
AclWI GGATC 2 cut(s) 491, 500
AcoI YGGCCR 2 cut(s) 78, 322
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 2 cut(s) 72, 345
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 2 cut(s) 383, 410
AgsI TTSAA 1 cut(s) 298
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 5 cut(s) 361, 408, 544, 550, 584
AluI AGCT 5 cut(s) 361, 408, 544, 550, 584
Alw21I GWGCWC 2 cut(s) 363, 546
Alw26I GTCTC 3 cut(s) 307, 352, 477
AlwI GGATC 2 cut(s) 491, 500
AoxI GGCC 5 cut(s) 78, 93, 181, 322, 412
ApeKI GCWGC 2 cut(s) 108, 584
ApoI RAATTY 1 cut(s) 233
AspLEI GCGC 2 cut(s) 74, 266
AspS9I GGNCC 3 cut(s) 20, 94, 283
AsuC2I CCSGG 2 cut(s) 239, 307
AsuHPI GGTGA 2 cut(s) 146, 391
AvaII GGWCC 2 cut(s) 20, 283
BaeGI GKGCMC 1 cut(s) 405
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 363, 546
Bbv12I GWGCWC 2 cut(s) 363, 546
BbvI GCAGC 2 cut(s) 120, 571
BccI CCATC 2 cut(s) 185, 544
BceAI ACGGC 1 cut(s) 59
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BciT130I CCWGG 1 cut(s) 215
BciVI GTATCC 1 cut(s) 398
BcnI CCSGG 2 cut(s) 239, 307
BcoDI GTCTC 3 cut(s) 307, 352, 477
BfoI RGCGCY 1 cut(s) 75
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 4 cut(s) 109, 229, 262, 585
BlsI GCNGC 4 cut(s) 110, 230, 263, 586
Bme1390I CCNGG 3 cut(s) 215, 239, 307
Bme18I GGWCC 2 cut(s) 20, 283
BmgT120I GGNCC 3 cut(s) 20, 94, 283
BmiI GGNNCC 3 cut(s) 21, 73, 96
BmrFI CCNGG 3 cut(s) 215, 239, 307
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 4 cut(s) 95, 129, 153, 207
BmuI ACTGGG 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 2 cut(s) 530, 536
BpuMI CCSGG 2 cut(s) 239, 307
BsaHI GRCGYC 2 cut(s) 72, 345
BsaJI CCNNGG 2 cut(s) 75, 213
BsaWI WCCGGW 1 cut(s) 376
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 410
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 2 cut(s) 75, 213
BseGI GGATG 1 cut(s) 403
BseLI CCNNNNNNNGG 2 cut(s) 383, 410
BseMII CTCAG 2 cut(s) 368, 531
BseNI ACTGG 1 cut(s) 12
BseRI GAGGAG 7 cut(s) 44, 141, 144, 147, 150, 176, 502
BseSI GKGCMC 1 cut(s) 405
BseX3I CGGCCG 1 cut(s) 322
BseXI GCAGC 2 cut(s) 120, 571
BseYI CCCAGC 2 cut(s) 252, 404
Bsh1236I CGCG 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 5 cut(s) 80, 95, 183, 324, 414
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 325
BsiHKAI GWGCWC 2 cut(s) 363, 546
BsiSI CCGG 8 cut(s) 81, 92, 160, 238, 306, 342, 377, 411
BslFI GGGAC 3 cut(s) 18, 322, 445
BslI CCNNNNNNNGG 2 cut(s) 383, 410
BsmAI GTCTC 3 cut(s) 307, 352, 477
BsmBI CGTCTC 2 cut(s) 307, 352
BsmFI GGGAC 3 cut(s) 18, 322, 445
BsnI GGCC 5 cut(s) 80, 95, 183, 324, 414
Bsp1286I GDGCHC 3 cut(s) 363, 405, 546
Bsp143I GATC 4 cut(s) 66, 151, 496, 505
BspACI CCGC 5 cut(s) 228, 262, 286, 351, 476
BspANI GGCC 5 cut(s) 80, 95, 183, 324, 414
BspCNI CTCAG 2 cut(s) 367, 532
BspFNI CGCG 1 cut(s) 264
BspLI GGNNCC 3 cut(s) 21, 73, 96
BspPI GGATC 2 cut(s) 491, 500
BspQI GCTCTTC 1 cut(s) 368
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 75, 213
BssMI GATC 4 cut(s) 66, 151, 496, 505
BssNI GRCGYC 2 cut(s) 72, 345
Bst2UI CCWGG 1 cut(s) 215
Bst6I CTCTTC 3 cut(s) 368, 470, 520
BstACI GRCGYC 2 cut(s) 72, 345
BstC8I GCNNGC 2 cut(s) 93, 173
BstDEI CTNAG 2 cut(s) 354, 540
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 1 cut(s) 403
BstFNI CGCG 1 cut(s) 264
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 266
BstKTI GATC 4 cut(s) 69, 154, 499, 508
BstMAI GTCTC 3 cut(s) 307, 352, 477
BstMBI GATC 4 cut(s) 66, 151, 496, 505
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 2 cut(s) 92, 117
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 3 cut(s) 213, 237, 305
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 120, 571
BstZI CGGCCG 1 cut(s) 322
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 5 cut(s) 80, 95, 183, 324, 414
BtgI CCRYGG 1 cut(s) 75
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 403
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 486
Cac8I GCNNGC 2 cut(s) 93, 173
CfoI GCGC 2 cut(s) 74, 266
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 3 cut(s) 20, 94, 283
CseI GACGC 1 cut(s) 334
Csp6I GTAC 1 cut(s) 577
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 2 cut(s) 354, 540
DinI GGCGCC 1 cut(s) 73
DpnI GATC 4 cut(s) 68, 153, 498, 507
DpnII GATC 4 cut(s) 66, 151, 496, 505
EaeI YGGCCR 2 cut(s) 78, 322
EagI CGGCCG 1 cut(s) 322
Eam1104I CTCTTC 3 cut(s) 368, 470, 520
EarI CTCTTC 3 cut(s) 368, 470, 520
EciI GGCGGA 1 cut(s) 465
Ecl136II GAGCTC 2 cut(s) 361, 544
EclXI CGGCCG 1 cut(s) 322
Eco24I GRGCYC 2 cut(s) 363, 546
Eco47I GGWCC 2 cut(s) 20, 283
Eco52I CGGCCG 1 cut(s) 322
Eco53kI GAGCTC 2 cut(s) 361, 544
EcoICRI GAGCTC 2 cut(s) 361, 544
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 213
EcoT38I GRGCYC 2 cut(s) 363, 546
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
Esp3I CGTCTC 2 cut(s) 307, 352
FaiI YATR 2 cut(s) 168, 592
FaqI GGGAC 3 cut(s) 18, 322, 445
Fnu4HI GCNGC 4 cut(s) 109, 229, 262, 585
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 2 cut(s) 363, 546
Fsp4HI GCNGC 4 cut(s) 109, 229, 262, 585
GlaI GCGC 2 cut(s) 73, 265
GluI GCNGC 4 cut(s) 109, 229, 262, 585
GsaI CCCAGC 2 cut(s) 256, 408
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 5 cut(s) 80, 95, 183, 324, 414
HapII CCGG 8 cut(s) 81, 92, 160, 238, 306, 342, 377, 411
HgaI GACGC 1 cut(s) 334
HhaI GCGC 2 cut(s) 74, 266
Hin1I GRCGYC 2 cut(s) 72, 345
Hin6I GCGC 2 cut(s) 72, 264
HinP1I GCGC 2 cut(s) 72, 264
HinfI GANTC 6 cut(s) 221, 277, 310, 327, 470, 556
HpaII CCGG 8 cut(s) 81, 92, 160, 238, 306, 342, 377, 411
HphI GGTGA 2 cut(s) 146, 391
Hpy188I TCNGA 5 cut(s) 125, 392, 505, 541, 565
Hpy188III TCNNGA 4 cut(s) 274, 356, 494, 553
HpyAV CCTTC 1 cut(s) 510
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 16, 486
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 117
HpyF3I CTNAG 2 cut(s) 354, 540
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 2 cut(s) 72, 345
HspAI GCGC 2 cut(s) 72, 264
KasI GGCGCC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 4 cut(s) 66, 151, 496, 505
LguI GCTCTTC 1 cut(s) 368
LmnI GCTCC 4 cut(s) 57, 83, 358, 413
Lsp1109I GCAGC 2 cut(s) 120, 571
LweI GCATC 4 cut(s) 95, 129, 153, 207
MaeII ACGT 1 cut(s) 300
MalI GATC 4 cut(s) 68, 153, 498, 507
MbiI CCGCTC 1 cut(s) 353
MboI GATC 4 cut(s) 66, 151, 496, 505
MboII GAAGA 8 cut(s) 38, 355, 457, 507, 510, 513, 516, 578
MhlI GDGCHC 3 cut(s) 363, 405, 546
MluCI AATT 2 cut(s) 233, 529
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 304, 479, 550
MroNI GCCGGC 1 cut(s) 91
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 8 cut(s) 81, 92, 160, 238, 306, 342, 377, 411
MspR9I CCNGG 3 cut(s) 215, 239, 307
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 92, 117
NaeI GCCGGC 1 cut(s) 93
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 2 cut(s) 239, 307
NdeII GATC 4 cut(s) 66, 151, 496, 505
NgoMIV GCCGGC 1 cut(s) 91
NlaIV GGNNCC 3 cut(s) 21, 73, 96
PciSI GCTCTTC 1 cut(s) 368
PcsI WCGNNNNNNNCGW 3 cut(s) 297, 432, 438
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 221, 277, 327
PfoI TCCNGGA 1 cut(s) 305
PkrI GCNGC 4 cut(s) 110, 230, 263, 586
PleI GAGTC 3 cut(s) 304, 478, 550
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 304, 478, 550
Psp124BI GAGCTC 2 cut(s) 363, 546
Psp6I CCWGG 1 cut(s) 213
PspFI CCCAGC 2 cut(s) 252, 404
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 3 cut(s) 21, 73, 96
PspPI GGNCC 3 cut(s) 20, 94, 283
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
SacI GAGCTC 2 cut(s) 363, 546
SapI GCTCTTC 1 cut(s) 368
SatI GCNGC 4 cut(s) 109, 229, 262, 585
Sau3AI GATC 4 cut(s) 66, 151, 496, 505
Sau96I GGNCC 3 cut(s) 20, 94, 283
SchI GAGTC 3 cut(s) 304, 479, 550
ScrFI CCNGG 3 cut(s) 215, 239, 307
SduI GDGCHC 3 cut(s) 363, 405, 546
SetI ASST 9 cut(s) 159, 285, 303, 363, 388, 410, 546, 552, 586
SfaNI GCATC 4 cut(s) 95, 129, 153, 207
SfoI GGCGCC 1 cut(s) 73
SinI GGWCC 2 cut(s) 20, 283
SmlI CTYRAG 2 cut(s) 545, 551
SmoI CTYRAG 2 cut(s) 545, 551
Sse9I AATT 2 cut(s) 233, 529
SsiI CCGC 5 cut(s) 228, 262, 286, 351, 476
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 209
SstI GAGCTC 2 cut(s) 363, 546
StyD4I CCNGG 3 cut(s) 213, 237, 305
TaiI ACGT 1 cut(s) 303
TaqI TCGA 4 cut(s) 273, 426, 441, 468
TasI AATT 2 cut(s) 233, 529
TauI GCSGC 2 cut(s) 231, 264
TfiI GAWTC 3 cut(s) 221, 277, 327
TscAI CASTG 1 cut(s) 493
TseI GCWGC 2 cut(s) 108, 584
TspDTI ATGAA 1 cut(s) 579
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 1 cut(s) 493
VpaK11BI GGWCC 2 cut(s) 20, 283
XapI RAATTY 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.