Rorug06G0148900

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000944
Physical Location & Seq
Forward (+)
21297033 .. 21301528
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0148900.1

Sequence Viewer

Length: 351 bp
ATGAGCGGAGTTTCAACAGCGGCGTACGTAGCTCGTCGGGCAGCTCAGAAGGAGCGGGTTCGGATCCTGTACCGCAAGGCCCTCAAAGACACTCTCAACTGGGCCGTCCACCGCCACCTCTTCTACCAAGATGCTGATGCTCTGCGCCAGAGGTTCGAGACCAACAAGCACGTGGAAGATCTTGACACAATCGATAGACTCATAGCTAATGCTGAAGCTACCTACGATAAGTGGCGCCACCCTGACCCTTATGTTGTTCCCTGGGCTCCTGGTGGTTCAAAGTTTCATCGGAATCCGACTCCACCTGCTGGGATTGAGATAGTATATGACTATGGCCGAGAAGATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

116

Amino Acids

13.48

Weight (kDa)

8.92

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 313
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 308
AccBSI CCGCTC 2 cut(s) 6, 55
AciI CCGC 5 cut(s) 6, 20, 55, 73, 112
AclWI GGATC 2 cut(s) 58, 71
AcoI YGGCCR 1 cut(s) 334
AcuI CTGAAG 1 cut(s) 234
AcvI CACGTG 1 cut(s) 172
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 2 cut(s) 26, 71
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 2 cut(s) 15, 279
AjnI CCWGG 2 cut(s) 260, 268
AluBI AGCT 4 cut(s) 32, 44, 206, 218
AluI AGCT 4 cut(s) 32, 44, 206, 218
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 2 cut(s) 58, 71
AoxI GGCC 3 cut(s) 78, 102, 334
ApeKI GCWGC 1 cut(s) 41
AspLEI GCGC 2 cut(s) 147, 237
AspS9I GGNCC 2 cut(s) 79, 102
BamHI GGATCC 1 cut(s) 63
BanI GGYRCC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 268
BbrPI CACGTG 1 cut(s) 172
BbvI GCAGC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 2 cut(s) 262, 270
BcoDI GTCTC 1 cut(s) 152
BfoI RGCGCY 1 cut(s) 238
BfuAI ACCTGC 1 cut(s) 313
BglII AGATCT 1 cut(s) 178
BisI GCNGC 2 cut(s) 21, 42
BlsI GCNGC 2 cut(s) 22, 43
Bme1390I CCNGG 2 cut(s) 262, 270
BmgT120I GGNCC 2 cut(s) 79, 102
BmiI GGNNCC 3 cut(s) 65, 236, 267
BmrFI CCNGG 2 cut(s) 262, 270
BmrI ACTGGG 1 cut(s) 109
BmsI GCATC 2 cut(s) 121, 127
BmuI ACTGGG 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 192
BsaAI YACGTR 2 cut(s) 28, 172
BsaHI GRCGYC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 260, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse1I ACTGG 1 cut(s) 104
BseBI CCWGG 2 cut(s) 262, 270
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 260, 261
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 53
BseYI CCCAGC 1 cut(s) 308
BshFI GGCC 3 cut(s) 80, 104, 336
BshNI GGYRCC 1 cut(s) 234
BshVI ATCGAT 1 cut(s) 192
BsiWI CGTACG 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 308
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 3 cut(s) 80, 104, 336
Bso31I GGTCTC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 2 cut(s) 63, 178
BspACI CCGC 5 cut(s) 6, 20, 55, 73, 112
BspANI GGCC 3 cut(s) 80, 104, 336
BspCNI CTCAG 1 cut(s) 58
BspDI ATCGAT 1 cut(s) 192
BspLI GGNNCC 3 cut(s) 65, 236, 267
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 2 cut(s) 58, 71
BspT107I GGYRCC 1 cut(s) 234
BspTNI GGTCTC 1 cut(s) 152
BsrBI CCGCTC 2 cut(s) 6, 55
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 2 cut(s) 260, 261
BssMI GATC 2 cut(s) 63, 178
BssNI GRCGYC 1 cut(s) 235
Bst2UI CCWGG 2 cut(s) 262, 270
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 235
BstBAI YACGTR 2 cut(s) 28, 172
BstDEI CTNAG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 2 cut(s) 147, 237
BstKTI GATC 2 cut(s) 66, 181
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 2 cut(s) 63, 178
BstMWI GCNNNNNNNGC 2 cut(s) 29, 38
BstNI CCWGG 2 cut(s) 262, 270
BstSCI CCNGG 2 cut(s) 260, 268
BstSNI TACGTA 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 2 cut(s) 63, 178
BstYI RGATCY 2 cut(s) 63, 178
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 3 cut(s) 80, 104, 336
BsuTUI ATCGAT 1 cut(s) 192
BveI ACCTGC 1 cut(s) 313
CfoI GCGC 2 cut(s) 147, 237
Cfr13I GGNCC 2 cut(s) 79, 102
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 2 cut(s) 25, 70
CviJI RGCY 8 cut(s) 32, 44, 80, 104, 206, 218, 266, 336
CviKI_1 RGCY 8 cut(s) 32, 44, 80, 104, 206, 218, 266, 336
CviQI GTAC 2 cut(s) 25, 70
DdeI CTNAG 1 cut(s) 45
DinI GGCGCC 1 cut(s) 236
DpnI GATC 2 cut(s) 65, 180
DpnII GATC 2 cut(s) 63, 178
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco105I TACGTA 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 268
Eco31I GGTCTC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 234
Eco72I CACGTG 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 2 cut(s) 260, 268
EcoT38I GRGCYC 1 cut(s) 268
EgeI GGCGCC 1 cut(s) 236
EheI GGCGCC 1 cut(s) 236
FaiI YATR 5 cut(s) 203, 252, 325, 327, 333
FauI CCCGC 1 cut(s) 48
Fnu4HI GCNGC 2 cut(s) 21, 42
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 2 cut(s) 21, 42
GlaI GCGC 2 cut(s) 146, 236
GluI GCNGC 2 cut(s) 21, 42
GsaI CCCAGC 1 cut(s) 312
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 3 cut(s) 80, 104, 336
HhaI GCGC 2 cut(s) 147, 237
Hin1I GRCGYC 1 cut(s) 235
Hin6I GCGC 2 cut(s) 145, 235
HinP1I GCGC 2 cut(s) 145, 235
HinfI GANTC 3 cut(s) 198, 292, 298
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 4 cut(s) 48, 63, 291, 297
Hpy188III TCNNGA 2 cut(s) 157, 182
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 39
HpyAV CCTTC 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 27, 171
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 38
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 2 cut(s) 27, 171
Hsp92I GRCGYC 1 cut(s) 235
HspAI GCGC 2 cut(s) 145, 235
KasI GGCGCC 1 cut(s) 234
Kzo9I GATC 2 cut(s) 63, 178
LmnI GCTCC 2 cut(s) 52, 271
Lsp1109I GCAGC 1 cut(s) 53
LweI GCATC 2 cut(s) 121, 127
MaeII ACGT 2 cut(s) 27, 171
MalI GATC 2 cut(s) 65, 180
MbiI CCGCTC 2 cut(s) 6, 55
MboI GATC 2 cut(s) 63, 178
MboII GAAGA 2 cut(s) 112, 188
MflI RGATCY 2 cut(s) 63, 178
MhlI GDGCHC 1 cut(s) 268
Mly113I GGCGCC 1 cut(s) 235
MlyI GAGTC 2 cut(s) 192, 292
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 3 cut(s) 92, 128, 144
MspA1I CMGCKG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 262, 270
MvaI CCWGG 2 cut(s) 262, 270
MwoI GCNNNNNNNGC 2 cut(s) 29, 38
NarI GGCGCC 1 cut(s) 235
NdeII GATC 2 cut(s) 63, 178
NlaIV GGNNCC 3 cut(s) 65, 236, 267
PaqCI CACCTGC 1 cut(s) 313
PasI CCCWGGG 1 cut(s) 261
PfeI GAWTC 1 cut(s) 292
Pfl23II CGTACG 1 cut(s) 24
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 2 cut(s) 22, 43
PleI GAGTC 2 cut(s) 192, 292
PluTI GGCGCC 1 cut(s) 238
PmaCI CACGTG 1 cut(s) 172
PmlI CACGTG 1 cut(s) 172
PpsI GAGTC 2 cut(s) 192, 292
Ppu21I YACGTR 2 cut(s) 28, 172
Psp6I CCWGG 2 cut(s) 260, 268
PspCI CACGTG 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 2 cut(s) 260, 268
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 65, 236, 267
PspPI GGNCC 2 cut(s) 79, 102
PsuI RGATCY 2 cut(s) 63, 178
RsaI GTAC 2 cut(s) 26, 71
RsaNI GTAC 2 cut(s) 25, 70
SatI GCNGC 2 cut(s) 21, 42
Sau3AI GATC 2 cut(s) 63, 178
Sau96I GGNCC 2 cut(s) 79, 102
SchI GAGTC 2 cut(s) 192, 292
ScrFI CCNGG 2 cut(s) 262, 270
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 2 cut(s) 121, 127
SfoI GGCGCC 1 cut(s) 236
SnaBI TACGTA 1 cut(s) 28
SsiI CCGC 5 cut(s) 6, 20, 55, 73, 112
SspDI GGCGCC 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 260, 268
TaiI ACGT 2 cut(s) 30, 174
TaqI TCGA 2 cut(s) 156, 192
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 1 cut(s) 292
TseI GCWGC 1 cut(s) 41
TspDTI ATGAA 1 cut(s) 275
Van91I CCANNNNNTGG 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.