RLG00000009314

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000944
Physical Location & Seq
Reverse (-)
50852971 .. 50853588
618 bp
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UTR
Exon/CDS
Intron
RLM00000009314

Sequence Viewer

Length: 462 bp
ATGTCACTGGGATTGCAGGGTTCAAAATCCCTCAGGGGCTTTGTTCTTCTGCTCTTCCTTGCTGCGATCGGTGCCGTGGCCGAAGCATCGCCGGCCCCAAAATCCAGTAACAAAACAAGCATCGGAGAAGGAGGAGGAAGAAGCATCTACGATCACCTCCAGCAATATAGAATTGCAGACTTATCCGACGTCTCCCCTCAGGAACTCTTCCTCTGGTTTTCGGTGAAAGGTATCCGAGTCGATGTGCCCAGCTCCGGCCTCATTTACTTCAATGTCGGCGTTGTCGATAAGCAGTTCTCTTTGTCCCTCTTCGAGTCTCCGCCCGATTGCACTGCTCTTGATCCTTCTTATCCCTCCTCTCCTTCTTCTTCTTCCAATTCCCCATCTGAGCTCAAGCTCAAGGTAGATTGTGATTCTGATTCCAATTCCAATTCTCAATTCGATTTCGTTGCTTCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

154

Amino Acids

16.29

Weight (kDa)

4.67

Isoelectric Point (pI)

64.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 51 - 105 1e-09 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 71
AciI CCGC 1 cut(s) 320
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 2 cut(s) 24, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AluBI AGCT 3 cut(s) 252, 391, 397
AluI AGCT 3 cut(s) 252, 391, 397
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 2 cut(s) 196, 321
AlwI GGATC 1 cut(s) 335
AoxI GGCC 3 cut(s) 78, 93, 256
ApeKI GCWGC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 94
AsuHPI GGTGA 2 cut(s) 146, 235
AxyI CCTNAGG 2 cut(s) 32, 198
BaeGI GKGCMC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 49
BccI CCATC 1 cut(s) 391
BceAI ACGGC 1 cut(s) 59
BcgI CGANNNNNNTGC 3 cut(s) 314, 348, 431
BciVI GTATCC 1 cut(s) 242
BcoDI GTCTC 2 cut(s) 196, 321
BfuI GTATCC 1 cut(s) 242
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 73, 96
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 3 cut(s) 95, 129, 153
BmuI ACTGGG 1 cut(s) 17
BpmI CTGGAG 1 cut(s) 143
BpuEI CTTGAG 2 cut(s) 377, 383
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 75
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 12, 105
Bse21I CCTNAGG 2 cut(s) 32, 198
BseDI CCNNGG 1 cut(s) 75
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 3 cut(s) 46, 212, 378
BseNI ACTGG 2 cut(s) 12, 105
BseRI GAGGAG 2 cut(s) 147, 346
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 248
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 3 cut(s) 80, 95, 258
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 2 cut(s) 92, 255
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 2 cut(s) 196, 321
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 3 cut(s) 80, 95, 258
Bsp1286I GDGCHC 2 cut(s) 249, 393
Bsp143I GATC 3 cut(s) 66, 151, 340
BspACI CCGC 1 cut(s) 320
BspANI GGCC 3 cut(s) 80, 95, 258
BspCNI CTCAG 3 cut(s) 45, 211, 379
BspLI GGNNCC 2 cut(s) 73, 96
BspPI GGATC 1 cut(s) 335
BspQI GCTCTTC 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 2 cut(s) 12, 105
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 3 cut(s) 66, 151, 340
BssNI GRCGYC 1 cut(s) 189
Bst6I CTCTTC 3 cut(s) 59, 212, 314
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 32, 198, 387
BstDSI CCRYGG 1 cut(s) 75
BstKTI GATC 3 cut(s) 69, 154, 343
BstMAI GTCTC 2 cut(s) 196, 321
BstMBI GATC 3 cut(s) 66, 151, 340
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 2 cut(s) 71, 92
BstSLI GKGCMC 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 49
Bsu36I CCTNAGG 2 cut(s) 32, 198
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 3 cut(s) 80, 95, 258
BtgI CCRYGG 1 cut(s) 75
BtgZI GCGATG 1 cut(s) 72
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 5, 330
Cac8I GCNNGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 94
CviJI RGCY 7 cut(s) 39, 80, 95, 252, 258, 391, 397
CviKI_1 RGCY 7 cut(s) 39, 80, 95, 252, 258, 391, 397
DdeI CTNAG 3 cut(s) 32, 198, 387
DpnI GATC 3 cut(s) 68, 153, 342
DpnII GATC 3 cut(s) 66, 151, 340
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 3 cut(s) 59, 212, 314
EarI CTCTTC 3 cut(s) 59, 212, 314
EciI GGCGGA 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 391
Eco81I CCTNAGG 2 cut(s) 32, 198
EcoICRI GAGCTC 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 393
Esp3I CGTCTC 1 cut(s) 196
FaiI YATR 1 cut(s) 168
FaqI GGGAC 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 63
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 1 cut(s) 63
GluI GCNGC 1 cut(s) 63
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 3 cut(s) 80, 95, 258
HapII CCGG 2 cut(s) 92, 255
Hin1I GRCGYC 1 cut(s) 189
HinfI GANTC 4 cut(s) 237, 314, 413, 419
HpaII CCGG 2 cut(s) 92, 255
HphI GGTGA 2 cut(s) 146, 235
Hpy188I TCNGA 5 cut(s) 125, 187, 236, 388, 418
Hpy188III TCNNGA 2 cut(s) 200, 338
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 122, 354, 372
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 3 cut(s) 16, 176, 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 92
HpyF3I CTNAG 3 cut(s) 32, 198, 387
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 3 cut(s) 66, 151, 340
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 1 cut(s) 257
LpnPI CCDG 9 cut(s) 2, 19, 105, 118, 173, 185, 199, 262, 268
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 3 cut(s) 95, 129, 153
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 3, 107
MalI GATC 3 cut(s) 68, 153, 342
MboI GATC 3 cut(s) 66, 151, 340
MboII GAAGA 8 cut(s) 38, 46, 150, 199, 301, 357, 360, 363
MhlI GDGCHC 2 cut(s) 249, 393
MluCI AATT 5 cut(s) 171, 376, 424, 430, 437
MlyI GAGTC 2 cut(s) 246, 323
MmeI TCCRAC 1 cut(s) 210
MroNI GCCGGC 1 cut(s) 91
MspI CCGG 2 cut(s) 92, 255
MwoI GCNNNNNNNGC 2 cut(s) 71, 92
NaeI GCCGGC 1 cut(s) 93
NdeII GATC 3 cut(s) 66, 151, 340
NgoMIV GCCGGC 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 73, 96
NmuCI GTSAC 1 cut(s) 3
PciSI GCTCTTC 1 cut(s) 59
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 2 cut(s) 413, 419
PkrI GCNGC 1 cut(s) 64
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 2 cut(s) 245, 322
PpsI GAGTC 2 cut(s) 245, 322
Psp124BI GAGCTC 1 cut(s) 393
PspFI CCCAGC 1 cut(s) 248
PspN4I GGNNCC 2 cut(s) 73, 96
PspPI GGNCC 1 cut(s) 94
PvuI CGATCG 1 cut(s) 69
SacI GAGCTC 1 cut(s) 393
SapI GCTCTTC 1 cut(s) 59
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 3 cut(s) 66, 151, 340
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 2 cut(s) 246, 323
SduI GDGCHC 2 cut(s) 249, 393
SetI ASST 7 cut(s) 159, 192, 232, 254, 393, 399, 405
SfaNI GCATC 3 cut(s) 95, 129, 153
SmlI CTYRAG 2 cut(s) 392, 398
SmoI CTYRAG 2 cut(s) 392, 398
Sse9I AATT 5 cut(s) 171, 376, 424, 430, 437
SsiI CCGC 1 cut(s) 320
SstI GAGCTC 1 cut(s) 393
TaiI ACGT 1 cut(s) 192
TaqI TCGA 5 cut(s) 240, 285, 312, 441, 455
TasI AATT 5 cut(s) 171, 376, 424, 430, 437
TfiI GAWTC 2 cut(s) 413, 419
TscAI CASTG 2 cut(s) 12, 337
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 3
TspRI CASTG 2 cut(s) 12, 337
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.