FvH4_6g00340

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000944
Physical Location & Seq
Reverse (-)
251072 .. 252763
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00340.t1

Sequence Viewer

Length: 603 bp
ATGGGATTGGGATTTCTTCTCCTTCCGCTTCTGGCGGTGATCGGCGCCGTGTCCGGAGCATCGCCGGCCCCGAAATCCAGCAAACGAAGCAGCGGAGGAGGAAGCATCTACGATCACCTCCGGCAATATGGGCTGCCCATCGGCATCCTCCCGAAAGGCATCACGAAATACTCCCTCGACGACACCAACGGCGAATTCCGGGTCTTCCTCCCGCAGCCCTGCCACGCCAAGTTCGAGAATCAGGTCCGCTACGATTTCAACGTCTCCGGCACTCTCTCCTTCGGCCGCATCGCCGATTTATCCGGCGTCTCCGCTCAGGAGCTCTTTCTTTGGTTTCCGGTCAAAGGCATCCGTGTCGATGTGCCTAGCTCCGGCCTCATTTACTTCGATGTCGGCGTCGTTGACAAGCAGTTCTCTTTGTCCCTCTTCGAGTCTCCGCCCGATTGTACTGCTCTCGATCCTTCTGATCCCTCCTCCTCCTCCTCTTCTTCTTCTAATTACGCATCTGCCCCTGCGTCCTCCTTCTCCTCTGACCTCAACCTCAAGCTCAAGAATGAACCGCAAGATGTTGGTGAAAGAAGTGAACTGAAGGCTGCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

201

Amino Acids

21.27

Weight (kDa)

5.35

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 36 - 144 3.8e-34 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AccBSI CCGCTC 1 cut(s) 314
AccIII TCCGGA 1 cut(s) 53
AciI CCGC 9 cut(s) 26, 35, 93, 212, 247, 286, 312, 437, 560
AclWI GGATC 2 cut(s) 452, 461
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 194
AcyI GRCGYC 3 cut(s) 45, 306, 396
AfaI GTAC 1 cut(s) 448
AfiI CCNNNNNNNGG 2 cut(s) 344, 371
AgsI TTSAA 1 cut(s) 259
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 3 cut(s) 322, 369, 547
AluI AGCT 3 cut(s) 322, 369, 547
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 3 cut(s) 268, 313, 438
AlwI GGATC 2 cut(s) 452, 461
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 3 cut(s) 66, 283, 373
ApeKI GCWGC 4 cut(s) 90, 133, 214, 593
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 2 cut(s) 67, 244
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 49, 107, 584
AvaII GGWCC 1 cut(s) 244
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 4 cut(s) 102, 120, 226, 580
BccI CCATC 1 cut(s) 146
BceAI ACGGC 2 cut(s) 32, 205
BcgI CGANNNNNNTGC 4 cut(s) 337, 371, 431, 465
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 3 cut(s) 268, 313, 438
BfaI CTAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 48
BisI GCNGC 5 cut(s) 91, 134, 215, 286, 594
BlsI GCNGC 5 cut(s) 92, 135, 216, 287, 595
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 67, 244
BmiI GGNNCC 2 cut(s) 46, 69
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 7 cut(s) 68, 114, 153, 168, 297, 357, 512
BpiI GAAGAC 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 315
BpuEI CTTGAG 2 cut(s) 527, 533
BpuMI CCSGG 1 cut(s) 200
BsaHI GRCGYC 3 cut(s) 45, 306, 396
BsaWI WCCGGW 2 cut(s) 53, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 371
Bse118I RCCGGY 1 cut(s) 64
BseAI TCCGGA 1 cut(s) 53
BseGI GGATG 2 cut(s) 144, 348
BseLI CCNNNNNNNGG 2 cut(s) 344, 371
BseMII CTCAG 1 cut(s) 329
BseRI GAGGAG 6 cut(s) 111, 463, 466, 469, 472, 517
BseX3I CGGCCG 1 cut(s) 283
BseXI GCAGC 4 cut(s) 102, 120, 226, 580
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 3 cut(s) 68, 285, 375
BshNI GGYRCC 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 8 cut(s) 54, 65, 121, 199, 267, 303, 338, 372
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 2 cut(s) 344, 371
BsmAI GTCTC 3 cut(s) 268, 313, 438
BsmBI CGTCTC 2 cut(s) 268, 313
BsmFI GGGAC 1 cut(s) 406
BsnI GGCC 3 cut(s) 68, 285, 375
Bsp1286I GDGCHC 1 cut(s) 324
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 4 cut(s) 39, 112, 457, 466
BspACI CCGC 9 cut(s) 26, 35, 93, 212, 247, 286, 312, 437, 560
BspANI GGCC 3 cut(s) 68, 285, 375
BspCNI CTCAG 1 cut(s) 328
BspEI TCCGGA 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 46, 69
BspPI GGATC 2 cut(s) 452, 461
BspT107I GGYRCC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 314
BsrFI RCCGGY 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 64
BssMI GATC 4 cut(s) 39, 112, 457, 466
BssNI GRCGYC 3 cut(s) 45, 306, 396
Bst6I CTCTTC 2 cut(s) 431, 490
BstACI GRCGYC 3 cut(s) 45, 306, 396
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 144, 348
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 4 cut(s) 42, 115, 460, 469
BstMAI GTCTC 3 cut(s) 268, 313, 438
BstMBI GATC 4 cut(s) 39, 112, 457, 466
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 3 cut(s) 65, 87, 130
BstSCI CCNGG 1 cut(s) 198
BstV1I GCAGC 4 cut(s) 102, 120, 226, 580
BstV2I GAAGAC 1 cut(s) 196
BstZI CGGCCG 1 cut(s) 283
BsuRI GGCC 3 cut(s) 68, 285, 375
BtgZI GCGATG 2 cut(s) 45, 274
BtsCI GGATG 2 cut(s) 144, 348
Cac8I GCNNGC 1 cut(s) 66
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 67, 244
CseI GACGC 3 cut(s) 295, 385, 504
Csp6I GTAC 1 cut(s) 447
CviJI RGCY 9 cut(s) 68, 133, 217, 285, 322, 369, 375, 547, 593
CviKI_1 RGCY 9 cut(s) 68, 133, 217, 285, 322, 369, 375, 547, 593
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 1 cut(s) 315
DinI GGCGCC 1 cut(s) 46
DpnI GATC 4 cut(s) 41, 114, 459, 468
DpnII GATC 4 cut(s) 39, 112, 457, 466
EaeI YGGCCR 1 cut(s) 283
EagI CGGCCG 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 431, 490
EarI CTCTTC 2 cut(s) 431, 490
EciI GGCGGA 1 cut(s) 426
Ecl136II GAGCTC 1 cut(s) 322
EclXI CGGCCG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 244
Eco52I CGGCCG 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 322
EcoRI GAATTC 1 cut(s) 194
EcoT38I GRGCYC 1 cut(s) 324
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
Esp3I CGTCTC 2 cut(s) 268, 313
FaiI YATR 2 cut(s) 129, 601
FaqI GGGAC 1 cut(s) 406
FauI CCCGC 1 cut(s) 219
Fnu4HI GCNGC 5 cut(s) 91, 134, 215, 286, 594
FokI GGATG 2 cut(s) 131, 335
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 5 cut(s) 91, 134, 215, 286, 594
FspBI CTAG 1 cut(s) 366
GlaI GCGC 1 cut(s) 46
GluI GCNGC 5 cut(s) 91, 134, 215, 286, 594
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 3 cut(s) 68, 285, 375
HapII CCGG 8 cut(s) 54, 65, 121, 199, 267, 303, 338, 372
HgaI GACGC 3 cut(s) 295, 385, 504
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 3 cut(s) 45, 306, 396
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 2 cut(s) 238, 431
HpaII CCGG 8 cut(s) 54, 65, 121, 199, 267, 303, 338, 372
HphI GGTGA 3 cut(s) 49, 107, 584
Hpy166II GTNNAC 2 cut(s) 403, 584
Hpy188I TCNGA 2 cut(s) 466, 532
Hpy188III TCNNGA 7 cut(s) 54, 151, 163, 235, 317, 455, 550
Hpy8I GTNNAC 2 cut(s) 403, 584
Hpy99I CGWCG 2 cut(s) 182, 401
HpyAV CCTTC 5 cut(s) 32, 289, 471, 532, 583
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 1 cut(s) 596
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 87, 130
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 3 cut(s) 45, 306, 396
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
Kpn2I TCCGGA 1 cut(s) 53
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Kzo9I GATC 4 cut(s) 39, 112, 457, 466
LmnI GCTCC 3 cut(s) 56, 319, 374
Lsp1109I GCAGC 4 cut(s) 102, 120, 226, 580
LweI GCATC 7 cut(s) 68, 114, 153, 168, 297, 357, 512
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 261
MalI GATC 4 cut(s) 41, 114, 459, 468
MbiI CCGCTC 1 cut(s) 314
MboI GATC 4 cut(s) 39, 112, 457, 466
MboII GAAGA 6 cut(s) 8, 196, 418, 477, 480, 483
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 2 cut(s) 194, 496
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 1 cut(s) 440
MroI TCCGGA 1 cut(s) 53
MroNI GCCGGC 1 cut(s) 64
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 8 cut(s) 54, 65, 121, 199, 267, 303, 338, 372
MspR9I CCNGG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 65, 87, 130
NaeI GCCGGC 1 cut(s) 66
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 200
NdeII GATC 4 cut(s) 39, 112, 457, 466
NgoMIV GCCGGC 1 cut(s) 64
NlaIV GGNNCC 2 cut(s) 46, 69
PcsI WCGNNNNNNNCGW 4 cut(s) 68, 231, 258, 393
PdiI GCCGGC 1 cut(s) 66
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 5 cut(s) 92, 135, 216, 287, 595
PleI GAGTC 1 cut(s) 439
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 439
Psp124BI GAGCTC 1 cut(s) 324
PspN4I GGNNCC 2 cut(s) 46, 69
PspPI GGNCC 2 cut(s) 67, 244
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
SacI GAGCTC 1 cut(s) 324
SatI GCNGC 5 cut(s) 91, 134, 215, 286, 594
Sau3AI GATC 4 cut(s) 39, 112, 457, 466
Sau96I GGNCC 2 cut(s) 67, 244
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 1 cut(s) 324
SetI ASST 8 cut(s) 120, 246, 264, 324, 371, 537, 543, 549
SfaNI GCATC 7 cut(s) 68, 114, 153, 168, 297, 357, 512
SfoI GGCGCC 1 cut(s) 46
SinI GGWCC 1 cut(s) 244
SmlI CTYRAG 2 cut(s) 542, 548
SmoI CTYRAG 2 cut(s) 542, 548
Sse9I AATT 2 cut(s) 194, 496
SsiI CCGC 9 cut(s) 26, 35, 93, 212, 247, 286, 312, 437, 560
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 366
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 198
TaiI ACGT 1 cut(s) 264
TaqI TCGA 6 cut(s) 177, 234, 357, 387, 429, 456
TasI AATT 2 cut(s) 194, 496
TatI WGTACW 1 cut(s) 446
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 1 cut(s) 238
TseI GCWGC 4 cut(s) 90, 133, 214, 593
TspDTI ATGAA 1 cut(s) 570
TspGWI ACGGA 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 244
XapI RAATTY 1 cut(s) 194
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.