Rorug05G0337800

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000944
Physical Location & Seq
Forward (+)
41015358 .. 41019954
4597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0337800.1

Sequence Viewer

Length: 456 bp
ATGAGTTACCTATTGACGACGCTAACGAAGAAGCAAGAGGTGGATTCGATCATCAGATCCACCATCGACAAGGTCCTCGTCCTCCGCTTCGGCCGCGCTTCCGATTCCGTCTCTCTTCTTCTCGACGACGTTCTCGCCAAATCAGCCCGTGACGTCTCCAAGTTCGCAACCGTGGCACTCGTCGATGTCGATTCCGACGACGTTCAGGTCTACCTCCATTACTTCGACATCACTTTGATTCCCTCCACCGTTTTCTTCTTCAACGCCCATCATATGAAGATGGATTCCGGAACTGCTGATCACACCAAATGGATCGGCGCGTTTCGGACGAAGCAGGACTTCATTGATGTCGTGGAGGCTATTTTCAGAGGAGCAATGAAAGGGAAGCTAATTGTGAACTGCCCCTTGCCGCCGGAACGAATTCCGAGATACCAGTTGTACTATAAGGATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

151

Amino Acids

17.18

Weight (kDa)

6.9

Isoelectric Point (pI)

27.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIM1 PF02966 4 - 137 2.7e-48 Mitosis protein DIM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AatII GACGTC 1 cut(s) 156
AccI GTMKAC 1 cut(s) 210
AccII CGCG 2 cut(s) 96, 320
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 3 cut(s) 85, 94, 410
AclWI GGATC 2 cut(s) 51, 320
AcoI YGGCCR 1 cut(s) 91
AcsI RAATTY 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 1 cut(s) 440
AgsI TTSAA 1 cut(s) 262
AluBI AGCT 1 cut(s) 388
AluI AGCT 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 115, 160
AlwI GGATC 2 cut(s) 51, 320
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 1 cut(s) 91
ApoI RAATTY 1 cut(s) 420
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 2 cut(s) 98, 320
AspS9I GGNCC 1 cut(s) 73
AvaII GGWCC 1 cut(s) 73
BccI CCATC 3 cut(s) 71, 274, 276
BclI TGATCA 1 cut(s) 298
BcoDI GTCTC 2 cut(s) 115, 160
BfaI CTAG 1 cut(s) 454
BisI GCNGC 2 cut(s) 94, 410
BlsI GCNGC 2 cut(s) 95, 411
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 381
BseAI TCCGGA 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 1 cut(s) 454
BseMI GCAATG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 384
BseX3I CGGCCG 1 cut(s) 91
Bsh1236I CGCG 2 cut(s) 96, 320
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 93
BsiEI CGRYCG 1 cut(s) 94
BsiSI CCGG 2 cut(s) 288, 413
BsmAI GTCTC 2 cut(s) 115, 160
BsmBI CGTCTC 2 cut(s) 115, 160
BsnI GGCC 1 cut(s) 93
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 4 cut(s) 48, 56, 298, 312
BspACI CCGC 3 cut(s) 85, 94, 410
BspANI GGCC 1 cut(s) 93
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 2 cut(s) 96, 320
BspPI GGATC 2 cut(s) 51, 320
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 4 cut(s) 48, 56, 298, 312
BssNI GRCGYC 1 cut(s) 153
Bst4CI ACNGT 2 cut(s) 172, 250
Bst6I CTCTTC 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 454
BstFNI CGCG 2 cut(s) 96, 320
BstHHI GCGC 2 cut(s) 98, 320
BstKTI GATC 4 cut(s) 51, 59, 301, 315
BstMAI GTCTC 2 cut(s) 115, 160
BstMBI GATC 4 cut(s) 48, 56, 298, 312
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 3 cut(s) 93, 143, 173
BstUI CGCG 2 cut(s) 96, 320
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BstZI CGGCCG 1 cut(s) 91
BsuRI GGCC 1 cut(s) 93
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 1 cut(s) 454
CfoI GCGC 2 cut(s) 98, 320
Cfr13I GGNCC 1 cut(s) 73
CseI GACGC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 439
CviJI RGCY 4 cut(s) 93, 146, 359, 388
CviKI_1 RGCY 4 cut(s) 93, 146, 359, 388
CviQI GTAC 1 cut(s) 439
DpnI GATC 4 cut(s) 50, 58, 300, 314
DpnII GATC 4 cut(s) 48, 56, 298, 312
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 91
EagI CGGCCG 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
EclXI CGGCCG 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 73
Eco52I CGGCCG 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 420
Esp3I CGTCTC 2 cut(s) 115, 160
FaiI YATR 3 cut(s) 273, 275, 444
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FauNDI CATATG 1 cut(s) 273
FbaI TGATCA 1 cut(s) 298
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 2 cut(s) 94, 410
Fsp4HI GCNGC 2 cut(s) 94, 410
FspBI CTAG 1 cut(s) 454
GlaI GCGC 2 cut(s) 97, 319
GluI GCNGC 2 cut(s) 94, 410
HaeIII GGCC 1 cut(s) 93
HapII CCGG 2 cut(s) 288, 413
HgaI GACGC 1 cut(s) 28
HhaI GCGC 2 cut(s) 98, 320
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 2 cut(s) 96, 318
HinP1I GCGC 2 cut(s) 96, 318
HinfI GANTC 5 cut(s) 44, 104, 191, 238, 284
HpaII CCGG 2 cut(s) 288, 413
Hpy166II GTNNAC 2 cut(s) 211, 397
Hpy188I TCNGA 6 cut(s) 56, 103, 196, 327, 368, 426
Hpy188III TCNNGA 2 cut(s) 122, 288
Hpy8I GTNNAC 2 cut(s) 211, 397
Hpy99I CGWCG 6 cut(s) 22, 128, 131, 185, 200, 203
HpyCH4III ACNGT 2 cut(s) 172, 250
HpyCH4IV ACGT 3 cut(s) 129, 153, 201
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 143, 173
HpySE526I ACGT 3 cut(s) 129, 153, 201
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 2 cut(s) 96, 318
Kpn2I TCCGGA 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 4 cut(s) 48, 56, 298, 312
LmnI GCTCC 1 cut(s) 371
LpnPI CCDG 5 cut(s) 191, 301, 320, 426, 446
MaeI CTAG 1 cut(s) 454
MaeII ACGT 3 cut(s) 129, 153, 201
MaeIII GTNAC 2 cut(s) 5, 149
MalI GATC 4 cut(s) 50, 58, 300, 314
MboI GATC 4 cut(s) 48, 56, 298, 312
MboII GAAGA 6 cut(s) 40, 107, 110, 247, 250, 289
MflI RGATCY 1 cut(s) 56
MluCI AATT 2 cut(s) 390, 420
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 7 cut(s) 31, 86, 92, 224, 253, 349, 362
MroI TCCGGA 1 cut(s) 287
MroXI GAANNNNTTC 1 cut(s) 420
MspI CCGG 2 cut(s) 288, 413
MvnI CGCG 2 cut(s) 96, 320
MwoI GCNNNNNNNGC 3 cut(s) 93, 143, 173
NdeI CATATG 1 cut(s) 273
NdeII GATC 4 cut(s) 48, 56, 298, 312
NmuCI GTSAC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 3 cut(s) 23, 186, 195
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 5 cut(s) 44, 104, 191, 238, 284
PflFI GACNNNGTC 1 cut(s) 71
PkrI GCNGC 2 cut(s) 95, 411
PpuMI RGGWCCY 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 73
PspPI GGNCC 1 cut(s) 73
PspPPI RGGWCCY 1 cut(s) 73
PsuI RGATCY 1 cut(s) 56
PsyI GACNNNGTC 1 cut(s) 71
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SatI GCNGC 2 cut(s) 94, 410
Sau3AI GATC 4 cut(s) 48, 56, 298, 312
Sau96I GGNCC 1 cut(s) 73
SetI ASST 9 cut(s) 12, 42, 75, 132, 156, 204, 210, 216, 390
SinI GGWCC 1 cut(s) 73
Sse9I AATT 2 cut(s) 390, 420
SsiI CCGC 3 cut(s) 85, 94, 410
SspMI CTAG 1 cut(s) 454
TaaI ACNGT 2 cut(s) 172, 250
TaiI ACGT 3 cut(s) 132, 156, 204
TaqI TCGA 6 cut(s) 47, 66, 123, 183, 189, 225
TasI AATT 2 cut(s) 390, 420
TatI WGTACW 1 cut(s) 438
TauI GCSGC 2 cut(s) 96, 412
TfiI GAWTC 5 cut(s) 44, 104, 191, 238, 284
TseFI GTSAC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 3 cut(s) 290, 331, 392
TspGWI ACGGA 1 cut(s) 97
Tth111I GACNNNGTC 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 1 cut(s) 454
ZraI GACGTC 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.