RLG00000025981

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000944
Physical Location & Seq
Forward (+)
49626911 .. 49628893
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025981

Sequence Viewer

Length: 597 bp
ATGGGACTATTGGGATTGCAGGGTCCAAAATCCCTTGTGGGCTTTGTTCTTCTGCTCCTCCTTGCTGTGATCGGAGCCGTGGCCGGAGCATCGCCGGCCTCCAAATCCAGCAGCAAAACAAGCATCGGAGGAGGAGGAGGAGGAAGCATCTACGATCACCTCAGGCAATATGGCTTACCCATCGGCCTCCTCCCCAAAGGCATCACGGAATATTCCCTCGACGATTCCAGCGGCGAATTCCGGGTGTTTCTGCCCCAGCCTTGCCGCGCCAAGTTCGAGAATCAGGTCCGCTACGATTTCAACGTCTCCGGGACTCTCACTTTCGGCCGAATCGCCGACTTGTCCGGCGTCTCCGCTCAGGAGCTCTTCCTTTGGTTTCCGGTGAAAGGTATCCGAGTGGATGTGCCCAGCTCCGGCCTCATTTACTTCGATGTCGGCGTTGTCGATAAGCAGTTCTCTTTGTCCCTCTTCGAGTCTCCGCCCGATTGCACTGCTCTTGATCCATCTGATCCCTCCTCCTCTTCTTCTTCCAATTCCCCATCTGAGCTCAAGCTCAAGACTCTATCACAAGATGTTGGGTACAAAGCGGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

199

Amino Acids

20.8

Weight (kDa)

5.37

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 50 - 158 2.9e-34 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 356
AccII CGCG 1 cut(s) 267
AciI CCGC 6 cut(s) 231, 265, 289, 354, 479, 587
AclWI GGATC 2 cut(s) 494, 503
AcoI YGGCCR 2 cut(s) 81, 325
AcsI RAATTY 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 2 cut(s) 386, 413
AgsI TTSAA 1 cut(s) 301
AluBI AGCT 4 cut(s) 364, 411, 547, 553
AluI AGCT 4 cut(s) 364, 411, 547, 553
Alw21I GWGCWC 2 cut(s) 366, 549
Alw26I GTCTC 3 cut(s) 310, 355, 480
AlwI GGATC 2 cut(s) 494, 503
AoxI GGCC 5 cut(s) 81, 96, 184, 325, 415
ApeKI GCWGC 1 cut(s) 111
ApoI RAATTY 1 cut(s) 236
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 2 cut(s) 23, 286
AsuC2I CCSGG 2 cut(s) 242, 310
AsuHPI GGTGA 2 cut(s) 149, 394
AvaII GGWCC 2 cut(s) 23, 286
AxyI CCTNAGG 1 cut(s) 161
BaeGI GKGCMC 1 cut(s) 408
BanII GRGCYC 2 cut(s) 366, 549
Bbv12I GWGCWC 2 cut(s) 366, 549
BbvI GCAGC 1 cut(s) 123
BccI CCATC 3 cut(s) 188, 511, 547
BceAI ACGGC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciVI GTATCC 1 cut(s) 401
BcnI CCSGG 2 cut(s) 242, 310
BcoDI GTCTC 3 cut(s) 310, 355, 480
BfuI GTATCC 1 cut(s) 401
BisI GCNGC 4 cut(s) 112, 232, 265, 588
BlsI GCNGC 4 cut(s) 113, 233, 266, 589
Bme1390I CCNGG 2 cut(s) 242, 310
Bme18I GGWCC 2 cut(s) 23, 286
BmgT120I GGNCC 2 cut(s) 23, 286
BmiI GGNNCC 2 cut(s) 24, 76
BmrFI CCNGG 2 cut(s) 242, 310
BmsI GCATC 4 cut(s) 98, 132, 156, 210
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 2 cut(s) 533, 539
BpuMI CCSGG 2 cut(s) 242, 310
BsaHI GRCGYC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 1 cut(s) 379
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 413
Bse118I RCCGGY 1 cut(s) 94
Bse21I CCTNAGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 2 cut(s) 386, 413
BseMII CTCAG 3 cut(s) 175, 371, 534
BseRI GAGGAG 8 cut(s) 47, 144, 147, 150, 153, 179, 505, 508
BseSI GKGCMC 1 cut(s) 408
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 123
BseYI CCCAGC 2 cut(s) 255, 407
Bsh1236I CGCG 1 cut(s) 267
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 5 cut(s) 83, 98, 186, 327, 417
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 2 cut(s) 366, 549
BsiSI CCGG 7 cut(s) 84, 95, 241, 309, 345, 380, 414
BslFI GGGAC 3 cut(s) 18, 325, 448
BslI CCNNNNNNNGG 2 cut(s) 386, 413
BsmAI GTCTC 3 cut(s) 310, 355, 480
BsmBI CGTCTC 2 cut(s) 310, 355
BsmFI GGGAC 3 cut(s) 18, 325, 448
BsnI GGCC 5 cut(s) 83, 98, 186, 327, 417
Bsp1286I GDGCHC 3 cut(s) 366, 408, 549
Bsp143I GATC 4 cut(s) 69, 154, 499, 508
BspACI CCGC 6 cut(s) 231, 265, 289, 354, 479, 587
BspANI GGCC 5 cut(s) 83, 98, 186, 327, 417
BspCNI CTCAG 3 cut(s) 174, 370, 535
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 24, 76
BspPI GGATC 2 cut(s) 494, 503
BspQI GCTCTTC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 4 cut(s) 69, 154, 499, 508
BssNI GRCGYC 1 cut(s) 348
Bst6I CTCTTC 3 cut(s) 371, 473, 526
BstACI GRCGYC 1 cut(s) 348
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 3 cut(s) 161, 357, 543
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 4 cut(s) 72, 157, 502, 511
BstMAI GTCTC 3 cut(s) 310, 355, 480
BstMBI GATC 4 cut(s) 69, 154, 499, 508
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 2 cut(s) 95, 120
BstSCI CCNGG 2 cut(s) 240, 308
BstSLI GKGCMC 1 cut(s) 408
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 123
BstZI CGGCCG 1 cut(s) 325
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 401
BsuRI GGCC 5 cut(s) 83, 98, 186, 327, 417
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 1 cut(s) 406
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 269
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 23, 286
CseI GACGC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 580
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 3 cut(s) 161, 357, 543
DpnI GATC 4 cut(s) 71, 156, 501, 510
DpnII GATC 4 cut(s) 69, 154, 499, 508
EaeI YGGCCR 2 cut(s) 81, 325
EagI CGGCCG 1 cut(s) 325
Eam1104I CTCTTC 3 cut(s) 371, 473, 526
EarI CTCTTC 3 cut(s) 371, 473, 526
EciI GGCGGA 1 cut(s) 468
Ecl136II GAGCTC 2 cut(s) 364, 547
EclXI CGGCCG 1 cut(s) 325
Eco24I GRGCYC 2 cut(s) 366, 549
Eco47I GGWCC 2 cut(s) 23, 286
Eco52I CGGCCG 1 cut(s) 325
Eco53kI GAGCTC 2 cut(s) 364, 547
Eco81I CCTNAGG 1 cut(s) 161
EcoICRI GAGCTC 2 cut(s) 364, 547
EcoRI GAATTC 1 cut(s) 236
EcoT38I GRGCYC 2 cut(s) 366, 549
Esp3I CGTCTC 2 cut(s) 310, 355
FaiI YATR 2 cut(s) 171, 595
FaqI GGGAC 3 cut(s) 18, 325, 448
Fnu4HI GCNGC 4 cut(s) 112, 232, 265, 588
FokI GGATG 1 cut(s) 413
FriOI GRGCYC 2 cut(s) 366, 549
Fsp4HI GCNGC 4 cut(s) 112, 232, 265, 588
GlaI GCGC 1 cut(s) 268
GluI GCNGC 4 cut(s) 112, 232, 265, 588
GsaI CCCAGC 2 cut(s) 259, 411
HaeIII GGCC 5 cut(s) 83, 98, 186, 327, 417
HapII CCGG 7 cut(s) 84, 95, 241, 309, 345, 380, 414
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 348
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HinfI GANTC 6 cut(s) 224, 280, 313, 330, 473, 559
HpaII CCGG 7 cut(s) 84, 95, 241, 309, 345, 380, 414
HphI GGTGA 2 cut(s) 149, 394
Hpy188I TCNGA 5 cut(s) 74, 128, 395, 508, 544
Hpy188III TCNNGA 4 cut(s) 277, 359, 497, 556
Hpy99I CGWCG 1 cut(s) 224
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 19, 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 120
HpyF3I CTNAG 3 cut(s) 161, 357, 543
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 1 cut(s) 348
HspAI GCGC 1 cut(s) 267
KroI GCCGGC 1 cut(s) 94
KroNI GCCGGC 1 cut(s) 96
Kzo9I GATC 4 cut(s) 69, 154, 499, 508
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 5 cut(s) 60, 74, 86, 361, 416
Lsp1109I GCAGC 1 cut(s) 123
LweI GCATC 4 cut(s) 98, 132, 156, 210
MaeII ACGT 1 cut(s) 303
MalI GATC 4 cut(s) 71, 156, 501, 510
MbiI CCGCTC 1 cut(s) 356
MboI GATC 4 cut(s) 69, 154, 499, 508
MboII GAAGA 6 cut(s) 41, 358, 460, 513, 516, 519
MhlI GDGCHC 3 cut(s) 366, 408, 549
MluCI AATT 2 cut(s) 236, 532
MlyI GAGTC 3 cut(s) 307, 482, 553
MroNI GCCGGC 1 cut(s) 94
MspA1I CMGCKG 1 cut(s) 231
MspI CCGG 7 cut(s) 84, 95, 241, 309, 345, 380, 414
MspR9I CCNGG 2 cut(s) 242, 310
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 95, 120
NaeI GCCGGC 1 cut(s) 96
NciI CCSGG 2 cut(s) 242, 310
NdeII GATC 4 cut(s) 69, 154, 499, 508
NgoMIV GCCGGC 1 cut(s) 94
NlaIV GGNNCC 2 cut(s) 24, 76
PciSI GCTCTTC 1 cut(s) 371
PcsI WCGNNNNNNNCGW 3 cut(s) 300, 435, 441
PdiI GCCGGC 1 cut(s) 96
PfeI GAWTC 3 cut(s) 224, 280, 330
PfoI TCCNGGA 1 cut(s) 308
PkrI GCNGC 4 cut(s) 113, 233, 266, 589
PleI GAGTC 3 cut(s) 307, 481, 553
PpsI GAGTC 3 cut(s) 307, 481, 553
Psp124BI GAGCTC 2 cut(s) 366, 549
PspFI CCCAGC 2 cut(s) 255, 407
PspN4I GGNNCC 2 cut(s) 24, 76
PspPI GGNCC 2 cut(s) 23, 286
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
SacI GAGCTC 2 cut(s) 366, 549
SapI GCTCTTC 1 cut(s) 371
SatI GCNGC 4 cut(s) 112, 232, 265, 588
Sau3AI GATC 4 cut(s) 69, 154, 499, 508
Sau96I GGNCC 2 cut(s) 23, 286
SchI GAGTC 3 cut(s) 307, 482, 553
ScrFI CCNGG 2 cut(s) 242, 310
SduI GDGCHC 3 cut(s) 366, 408, 549
SetI ASST 8 cut(s) 162, 288, 306, 366, 391, 413, 549, 555
SfaNI GCATC 4 cut(s) 98, 132, 156, 210
SinI GGWCC 2 cut(s) 23, 286
SmlI CTYRAG 2 cut(s) 548, 554
SmoI CTYRAG 2 cut(s) 548, 554
Sse9I AATT 2 cut(s) 236, 532
SsiI CCGC 6 cut(s) 231, 265, 289, 354, 479, 587
SspI AATATT 1 cut(s) 212
SstI GAGCTC 2 cut(s) 366, 549
StyD4I CCNGG 2 cut(s) 240, 308
TaiI ACGT 1 cut(s) 306
TaqI TCGA 5 cut(s) 219, 276, 429, 444, 471
TasI AATT 2 cut(s) 236, 532
TauI GCSGC 3 cut(s) 234, 267, 590
TfiI GAWTC 3 cut(s) 224, 280, 330
TscAI CASTG 1 cut(s) 496
TseI GCWGC 1 cut(s) 111
TspDTI ATGAA 1 cut(s) 582
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 1 cut(s) 496
VpaK11BI GGWCC 2 cut(s) 23, 286
XapI RAATTY 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.