O-methyltransferase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 329 |
| AciI | CCGC | 1 cut(s) | 327 |
| AclWI | GGATC | 2 cut(s) | 728, 779 |
| AcsI | RAATTY | 1 cut(s) | 354 |
| AcuI | CTGAAG | 2 cut(s) | 363, 424 |
| AdeI | CACNNNGTG | 1 cut(s) | 612 |
| AfaI | GTAC | 1 cut(s) | 659 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 347 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 374, 406 |
| AluBI | AGCT | 3 cut(s) | 230, 265, 691 |
| AluI | AGCT | 3 cut(s) | 230, 265, 691 |
| Alw26I | GTCTC | 4 cut(s) | 69, 107, 134, 669 |
| AlwI | GGATC | 2 cut(s) | 728, 779 |
| AoxI | GGCC | 1 cut(s) | 448 |
| ApeKI | GCWGC | 1 cut(s) | 707 |
| ApoI | RAATTY | 1 cut(s) | 354 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 500, 532 |
| AspS9I | GGNCC | 1 cut(s) | 449 |
| AsuHPI | GGTGA | 1 cut(s) | 758 |
| BbsI | GAAGAC | 2 cut(s) | 35, 351 |
| BbvI | GCAGC | 1 cut(s) | 694 |
| BccI | CCATC | 1 cut(s) | 743 |
| BceAI | ACGGC | 2 cut(s) | 364, 750 |
| BciVI | GTATCC | 1 cut(s) | 162 |
| BcoDI | GTCTC | 4 cut(s) | 69, 107, 134, 669 |
| BfaI | CTAG | 1 cut(s) | 156 |
| BfmI | CTRYAG | 1 cut(s) | 670 |
| BfuI | GTATCC | 1 cut(s) | 162 |
| BglII | AGATCT | 1 cut(s) | 726 |
| BisI | GCNGC | 1 cut(s) | 708 |
| BlsI | GCNGC | 1 cut(s) | 709 |
| BmgT120I | GGNCC | 1 cut(s) | 449 |
| BmiI | GGNNCC | 1 cut(s) | 633 |
| BmsI | GCATC | 2 cut(s) | 514, 631 |
| BpiI | GAAGAC | 2 cut(s) | 35, 351 |
| BpmI | CTGGAG | 1 cut(s) | 158 |
| BpuEI | CTTGAG | 1 cut(s) | 707 |
| Bsa29I | ATCGAT | 1 cut(s) | 567 |
| BsaBI | GATNNNNATC | 1 cut(s) | 725 |
| BsaJI | CCNNGG | 3 cut(s) | 206, 496, 716 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 347 |
| Bse118I | RCCGGY | 1 cut(s) | 416 |
| Bse1I | ACTGG | 3 cut(s) | 127, 141, 310 |
| Bse8I | GATNNNNATC | 1 cut(s) | 725 |
| BseCI | ATCGAT | 1 cut(s) | 567 |
| BseDI | CCNNGG | 3 cut(s) | 206, 496, 716 |
| BseGI | GGATG | 2 cut(s) | 202, 373 |
| BseJI | GATNNNNATC | 1 cut(s) | 725 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 347 |
| BseMII | CTCAG | 2 cut(s) | 80, 196 |
| BseNI | ACTGG | 3 cut(s) | 127, 141, 310 |
| BseRI | GAGGAG | 2 cut(s) | 600, 624 |
| BseXI | GCAGC | 1 cut(s) | 694 |
| BshFI | GGCC | 1 cut(s) | 450 |
| BshVI | ATCGAT | 1 cut(s) | 567 |
| BsiSI | CCGG | 1 cut(s) | 417 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 347 |
| BsmAI | GTCTC | 4 cut(s) | 69, 107, 134, 669 |
| BsnI | GGCC | 1 cut(s) | 450 |
| Bsp19I | CCATGG | 2 cut(s) | 206, 496 |
| BspACI | CCGC | 1 cut(s) | 327 |
| BspANI | GGCC | 1 cut(s) | 450 |
| BspCNI | CTCAG | 2 cut(s) | 79, 195 |
| BspDI | ATCGAT | 1 cut(s) | 567 |
| BspLI | GGNNCC | 1 cut(s) | 633 |
| BspPI | GGATC | 2 cut(s) | 728, 779 |
| BspQI | GCTCTTC | 1 cut(s) | 462 |
| BsrBI | CCGCTC | 1 cut(s) | 329 |
| BsrFI | RCCGGY | 1 cut(s) | 416 |
| BsrI | ACTGG | 3 cut(s) | 127, 141, 310 |
| BssAI | RCCGGY | 1 cut(s) | 416 |
| BssECI | CCNNGG | 3 cut(s) | 206, 496, 716 |
| BssT1I | CCWWGG | 2 cut(s) | 206, 496 |
| Bst4CI | ACNGT | 1 cut(s) | 243 |
| Bst6I | CTCTTC | 2 cut(s) | 321, 462 |
| BstC8I | GCNNGC | 1 cut(s) | 418 |
| BstDEI | CTNAG | 2 cut(s) | 66, 182 |
| BstDSI | CCRYGG | 2 cut(s) | 206, 496 |
| BstF5I | GGATG | 2 cut(s) | 202, 373 |
| BstMAI | GTCTC | 4 cut(s) | 69, 107, 134, 669 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 262, 422, 707 |
| BstNSI | RCATGY | 1 cut(s) | 256 |
| BstSFI | CTRYAG | 1 cut(s) | 670 |
| BstV1I | GCAGC | 1 cut(s) | 694 |
| BstV2I | GAAGAC | 2 cut(s) | 35, 351 |
| BstX2I | RGATCY | 1 cut(s) | 726 |
| BstYI | RGATCY | 1 cut(s) | 726 |
| Bsu15I | ATCGAT | 1 cut(s) | 567 |
| BsuI | GTATCC | 1 cut(s) | 162 |
| BsuRI | GGCC | 1 cut(s) | 450 |
| BsuTUI | ATCGAT | 1 cut(s) | 567 |
| BtgI | CCRYGG | 2 cut(s) | 206, 496 |
| BtgZI | GCGATG | 1 cut(s) | 129 |
| BtsCI | GGATG | 2 cut(s) | 202, 373 |
| BtsIMutI | CAGTG | 1 cut(s) | 303 |
| Cac8I | GCNNGC | 1 cut(s) | 418 |
| Cfr10I | RCCGGY | 1 cut(s) | 416 |
| Cfr13I | GGNCC | 1 cut(s) | 449 |
| ClaI | ATCGAT | 1 cut(s) | 567 |
| Csp6I | GTAC | 1 cut(s) | 658 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 313, 348 |
| CviAII | CATG | 3 cut(s) | 207, 253, 497 |
| CviQI | GTAC | 1 cut(s) | 658 |
| DdeI | CTNAG | 2 cut(s) | 66, 182 |
| DraIII | CACNNNGTG | 1 cut(s) | 612 |
| Eam1104I | CTCTTC | 2 cut(s) | 321, 462 |
| EarI | CTCTTC | 2 cut(s) | 321, 462 |
| Eco130I | CCWWGG | 2 cut(s) | 206, 496 |
| Eco57I | CTGAAG | 2 cut(s) | 363, 424 |
| EcoO109I | RGGNCCY | 1 cut(s) | 449 |
| EcoT14I | CCWWGG | 2 cut(s) | 206, 496 |
| ErhI | CCWWGG | 2 cut(s) | 206, 496 |
| FaeI | CATG | 3 cut(s) | 210, 256, 500 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 89, 121, 422, 454 |
| FatI | CATG | 3 cut(s) | 206, 252, 496 |
| FauNDI | CATATG | 1 cut(s) | 505 |
| Fnu4HI | GCNGC | 1 cut(s) | 708 |
| FokI | GGATG | 2 cut(s) | 189, 380 |
| Fsp4HI | GCNGC | 1 cut(s) | 708 |
| FspBI | CTAG | 1 cut(s) | 156 |
| GluI | GCNGC | 1 cut(s) | 708 |
| GsuI | CTGGAG | 1 cut(s) | 158 |
| HaeIII | GGCC | 1 cut(s) | 450 |
| HapII | CCGG | 1 cut(s) | 417 |
| Hin1II | CATG | 3 cut(s) | 210, 256, 500 |
| HincII | GTYRAC | 2 cut(s) | 271, 373 |
| HindII | GTYRAC | 2 cut(s) | 271, 373 |
| HinfI | GANTC | 4 cut(s) | 70, 100, 167, 753 |
| HpaI | GTTAAC | 1 cut(s) | 271 |
| HpaII | CCGG | 1 cut(s) | 417 |
| HphI | GGTGA | 1 cut(s) | 758 |
| Hpy166II | GTNNAC | 4 cut(s) | 271, 301, 373, 398 |
| Hpy188I | TCNGA | 5 cut(s) | 57, 69, 296, 720, 771 |
| Hpy188III | TCNNGA | 5 cut(s) | 83, 467, 638, 686, 724 |
| Hpy8I | GTNNAC | 4 cut(s) | 271, 301, 373, 398 |
| Hpy99I | CGWCG | 2 cut(s) | 10, 771 |
| HpyAV | CCTTC | 3 cut(s) | 169, 230, 440 |
| HpyCH4III | ACNGT | 1 cut(s) | 243 |
| HpyCH4IV | ACGT | 1 cut(s) | 660 |
| HpyCH4V | TGCA | 1 cut(s) | 644 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 262, 422, 707 |
| HpyF3I | CTNAG | 2 cut(s) | 66, 182 |
| HpySE526I | ACGT | 1 cut(s) | 660 |
| Hsp92II | CATG | 3 cut(s) | 210, 256, 500 |
| KroI | GCCGGC | 1 cut(s) | 416 |
| KroNI | GCCGGC | 1 cut(s) | 418 |
| KspAI | GTTAAC | 1 cut(s) | 271 |
| LguI | GCTCTTC | 1 cut(s) | 462 |
| LmnI | GCTCC | 2 cut(s) | 637, 741 |
| Lsp1109I | GCAGC | 1 cut(s) | 694 |
| LweI | GCATC | 2 cut(s) | 514, 631 |
| MaeI | CTAG | 1 cut(s) | 156 |
| MaeII | ACGT | 1 cut(s) | 660 |
| MaeIII | GTNAC | 3 cut(s) | 92, 190, 665 |
| MbiI | CCGCTC | 1 cut(s) | 329 |
| MboII | GAAGA | 7 cut(s) | 40, 62, 65, 308, 356, 449, 502 |
| MflI | RGATCY | 1 cut(s) | 726 |
| MluCI | AATT | 4 cut(s) | 354, 382, 389, 579 |
| MlyI | GAGTC | 1 cut(s) | 109 |
| MmeI | TCCRAC | 1 cut(s) | 749 |
| MnlI | CCTC | 4 cut(s) | 578, 645, 645, 726 |
| MroNI | GCCGGC | 1 cut(s) | 416 |
| MseI | TTAA | 3 cut(s) | 248, 270, 693 |
| MspA1I | CMGCKG | 1 cut(s) | 230 |
| MspI | CCGG | 1 cut(s) | 417 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 262, 422, 707 |
| NaeI | GCCGGC | 1 cut(s) | 418 |
| NcoI | CCATGG | 2 cut(s) | 206, 496 |
| NdeI | CATATG | 1 cut(s) | 505 |
| NgoMIV | GCCGGC | 1 cut(s) | 416 |
| NlaIII | CATG | 3 cut(s) | 210, 256, 500 |
| NlaIV | GGNNCC | 1 cut(s) | 633 |
| NmuCI | GTSAC | 2 cut(s) | 92, 190 |
| NspI | RCATGY | 1 cut(s) | 256 |
| PciSI | GCTCTTC | 1 cut(s) | 462 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 483 |
| PdiI | GCCGGC | 1 cut(s) | 418 |
| PfeI | GAWTC | 3 cut(s) | 70, 167, 753 |
| PkrI | GCNGC | 1 cut(s) | 709 |
| PleI | GAGTC | 1 cut(s) | 108 |
| PpsI | GAGTC | 1 cut(s) | 108 |
| PspN4I | GGNNCC | 1 cut(s) | 633 |
| PspPI | GGNCC | 1 cut(s) | 449 |
| PsuI | RGATCY | 1 cut(s) | 726 |
| PvuII | CAGCTG | 1 cut(s) | 230 |
| RsaI | GTAC | 1 cut(s) | 659 |
| RsaNI | GTAC | 1 cut(s) | 658 |
| SapI | GCTCTTC | 1 cut(s) | 462 |
| SaqAI | TTAA | 3 cut(s) | 248, 270, 693 |
| SatI | GCNGC | 1 cut(s) | 708 |
| Sau96I | GGNCC | 1 cut(s) | 449 |
| SchI | GAGTC | 1 cut(s) | 109 |
| SetI | ASST | 4 cut(s) | 232, 267, 663, 693 |
| SfaNI | GCATC | 2 cut(s) | 514, 631 |
| SfcI | CTRYAG | 1 cut(s) | 670 |
| SmlI | CTYRAG | 1 cut(s) | 686 |
| SmoI | CTYRAG | 1 cut(s) | 686 |
| Sse9I | AATT | 4 cut(s) | 354, 382, 389, 579 |
| SsiI | CCGC | 1 cut(s) | 327 |
| SspMI | CTAG | 1 cut(s) | 156 |
| StyI | CCWWGG | 2 cut(s) | 206, 496 |
| TaaI | ACNGT | 1 cut(s) | 243 |
| TaiI | ACGT | 1 cut(s) | 663 |
| TaqI | TCGA | 5 cut(s) | 38, 76, 435, 567, 723 |
| TasI | AATT | 4 cut(s) | 354, 382, 389, 579 |
| TfiI | GAWTC | 3 cut(s) | 70, 167, 753 |
| Tru1I | TTAA | 3 cut(s) | 248, 270, 693 |
| Tru9I | TTAA | 3 cut(s) | 248, 270, 693 |
| TscAI | CASTG | 1 cut(s) | 310 |
| TseFI | GTSAC | 2 cut(s) | 92, 190 |
| TseI | GCWGC | 1 cut(s) | 707 |
| Tsp45I | GTSAC | 2 cut(s) | 92, 190 |
| TspDTI | ATGAA | 3 cut(s) | 188, 249, 486 |
| TspRI | CASTG | 1 cut(s) | 310 |
| XapI | RAATTY | 1 cut(s) | 354 |
| XceI | RCATGY | 1 cut(s) | 256 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 283 |
| XspI | CTAG | 1 cut(s) | 156 |