Rroxscaffold_5G00382650

caffeoyl-CoA O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000432
Physical Location & Seq
Reverse (-)
62511755 .. 62512921
1167 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00382650.1

Sequence Viewer

Length: 531 bp
ATGGCCATGCTCTTGAAACTTGTGAATGCAAAGAAGACAATTGAAGTTGGAGTTTTGACTGGATACTCTCTTCTCCTTACGGCTCTCACAATCCCTGACGACGGCAAGATTATGGCCATAGATAGAAATCGTAAAACATATGAAATAGGCCTCCCAATCATACAAAAAGCCGGAGTTGAGTACAAAATCGACTACATCGAATCCCCGGCTCTGCCTGTGCTTGACAATCTCTTGGGAGAACCAAAGAATGAGGGTGATTTCGACTTTGCATTTGTCGATGCTGACAAAGATAACTATTGGAATTATCATAAGAGGCTGATGAAACTGGTGAAGATTGGTGGGATTGTTATGTATGATAACACACTGTGGGGAGGGGCAGTGGCTAAGCCTGAAGACGCTGTTCCGGAAGCCAAAAGGGAGCTGAGGCGGGCTACAATTGAGTTTAACAAGTCAGTTTCGGCTGACCCTCGAGTTGAAATCTCTCATGCATCTGTGGGAGATGGAATCATCATCTGCAGACGCATTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00360 ko00940 ko00941 ko00945 ko01100 ko01110 map00360 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Methyltransf_3

Protein Analysis

176

Amino Acids

19.61

Weight (kDa)

6.15

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_3 PF01596 2 - 175 2.9e-68 O-methyltransferase
Methyltransf_24 PF13578 14 - 120 3.2e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000432)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34050 AT4G34050 AT4G34050
fragaria_vesca FvH4_1g06851 FvH4_1g06860 FvH4_1g06860 FvH4_1g06890 FvH4_1g06890 FvH4_1g13960 FvH4_1g13960 FvH4_1g13960 FvH4_1g13960 FvH4_1g14010 FvH4_1g14010 FvH4_1g14010 FvH4_1g14010 FvH4_1g14010 FvH4_2g05780 FvH4_2g05780
malus_domestica MD00G1088100.v1.1 MD02G1073300.v1.1 MD05G1083900.v1.1
prunus_persica Prupe.7G214300_v2.0.a1 Prupe.7G214300_v2.0.a1 Prupe.7G214300_v2.0.a1 Prupe.8G128100_v2.0.a1 Prupe.8G128100_v2.0.a1 Prupe.8G128100_v2.0.a1 Prupe.8G128100_v2.0.a1
pyrus_communis pycom02g05780 pycom05g08090 pycom10g08030
rosa_chinensis RchiOBHm_Chr2g0092641 RchiOBHm_Chr2g0092651 RchiOBHm_Chr2g0092661 RchiOBHm_Chr2g0092671 RchiOBHm_Chr2g0092711 RchiOBHm_Chr2g0092721 RchiOBHm_Chr2g0102321 RchiOBHm_Chr3g0467881 RchiOBHm_Chr6g0256691
rosa_laevigata RLG00000014741 RLG00000016330 RLG00000016331 RLG00000016332 RLG00000016334 RLG00000016335 RLG00000029637
rosa_multiflora Rmu_sc0001132.1_g000016 Rmu_sc0001132.1_g000022 Rmu_sc0001793.1_g000037 Rmu_sc0005055.1_g000009 Rmu_sc0010161.1_g000022 Rmu_sc0017518.1_g000002 Rmu_sc0017518.1_g000003 Rmu_sc0032103.1_g000001
rosa_roxburghii Rroxscaffold_2G00140570 Rroxscaffold_5G00382650 Rroxscaffold_6G00413380 Rroxscaffold_7G00209130
rosa_rugosa Rorug02G0028400 Rorug02G0028900 Rorug02G0029000 Rorug02G0029100 Rorug02G0106500 Rorug05G0266900 Rorug05G0565700
rosa_samantha Rh1BG085800 Rh2AG074200 Rh2AG074300 Rh2AG074400 Rh2AG074600 Rh2AG154600 Rh2BG160700 Rh2CG076900 Rh2CG077000 Rh2CG077100 Rh2CG077300 Rh2CG077400 Rh2CG077500 Rh2CG160400 Rh3BG159900 Rh3CG160000 Rh3DG160600 Rh5CG376400 Rh6AG083700 Rh6BG078200 Rh6CG072800 Rh6DG070000
rosa_wichuraiana Rw2G005850 Rw2G005870 Rw2G005880 Rw2G012080 Rw3G013130 Rw6G007520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 403
AciI CCGC 1 cut(s) 427
AcoI YGGCCR 2 cut(s) 3, 114
AcuI CTGAAG 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 366
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 3 cut(s) 16, 44, 476
AluBI AGCT 1 cut(s) 421
AluI AGCT 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 468
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 3 cut(s) 3, 114, 148
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 266, 340
AvaI CYCGRG 1 cut(s) 468
BalI TGGCCA 2 cut(s) 5, 116
BbsI GAAGAC 2 cut(s) 41, 399
BbvCI CCTCAGC 1 cut(s) 422
BccI CCATC 1 cut(s) 494
BceAI ACGGC 2 cut(s) 96, 118
BciVI GTATCC 1 cut(s) 56
BcnI CCSGG 1 cut(s) 206
BfmI CTRYAG 1 cut(s) 514
BfuI GTATCC 1 cut(s) 56
BlpI GCTNAGC 1 cut(s) 384
Bme1390I CCNGG 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 2 cut(s) 268, 497
BpiI GAAGAC 2 cut(s) 41, 399
Bpu10I CCTNAGC 1 cut(s) 422
Bpu1102I GCTNAGC 1 cut(s) 384
BpuMI CCSGG 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 204
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 64, 330
Bse8I GATNNNNATC 1 cut(s) 126
BseAI TCCGGA 1 cut(s) 403
BseDI CCNNGG 1 cut(s) 204
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 2 cut(s) 64, 330
BshFI GGCC 3 cut(s) 5, 116, 150
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 3 cut(s) 171, 206, 404
BslI CCNNNNNNNGG 1 cut(s) 101
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 3 cut(s) 5, 116, 150
BsoBI CYCGRG 1 cut(s) 468
Bsp13I TCCGGA 1 cut(s) 403
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 1 cut(s) 427
BspANI GGCC 3 cut(s) 5, 116, 150
BspCNI CTCAG 1 cut(s) 414
BspEI TCCGGA 1 cut(s) 403
BspMAI CTGCAG 1 cut(s) 518
BsrI ACTGG 2 cut(s) 64, 330
BssECI CCNNGG 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 366
Bst6I CTCTTC 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 2 cut(s) 384, 422
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 514
BstV2I GAAGAC 2 cut(s) 41, 399
BsuI GTATCC 1 cut(s) 56
BsuRI GGCC 3 cut(s) 5, 116, 150
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 2 cut(s) 362, 384
Cac8I GCNNGC 1 cut(s) 429
CseI GACGC 1 cut(s) 404
Csp6I GTAC 1 cut(s) 181
CviAII CATG 2 cut(s) 7, 485
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 2 cut(s) 384, 422
DraIII CACNNNGTG 1 cut(s) 366
EaeI YGGCCR 2 cut(s) 3, 114
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 468
EcoT22I ATGCAT 1 cut(s) 490
FaeI CATG 2 cut(s) 10, 488
FatI CATG 2 cut(s) 6, 484
FauI CCCGC 1 cut(s) 420
FauNDI CATATG 1 cut(s) 139
HaeIII GGCC 3 cut(s) 5, 116, 150
HapII CCGG 3 cut(s) 171, 206, 404
HgaI GACGC 1 cut(s) 404
Hin1II CATG 2 cut(s) 10, 488
HinfI GANTC 2 cut(s) 200, 504
HpaII CCGG 3 cut(s) 171, 206, 404
HphI GGTGA 2 cut(s) 266, 340
Hpy188III TCNNGA 2 cut(s) 13, 404
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 29, 269, 488, 516
HpyF3I CTNAG 2 cut(s) 384, 422
Hsp92II CATG 2 cut(s) 10, 488
Kpn2I TCCGGA 1 cut(s) 403
LmnI GCTCC 1 cut(s) 418
LpnPI CCDG 8 cut(s) 45, 108, 184, 219, 228, 311, 402, 417
LweI GCATC 2 cut(s) 268, 497
MboII GAAGA 4 cut(s) 46, 62, 343, 404
MfeI CAATTG 2 cut(s) 39, 435
MlsI TGGCCA 2 cut(s) 5, 116
MluCI AATT 3 cut(s) 39, 301, 435
MluNI TGGCCA 2 cut(s) 5, 116
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 6 cut(s) 161, 244, 306, 365, 417, 477
Mox20I TGGCCA 2 cut(s) 5, 116
Mph1103I ATGCAT 1 cut(s) 490
MroI TCCGGA 1 cut(s) 403
MscI TGGCCA 2 cut(s) 5, 116
MseI TTAA 1 cut(s) 444
Msp20I TGGCCA 2 cut(s) 5, 116
MspI CCGG 3 cut(s) 171, 206, 404
MspR9I CCNGG 1 cut(s) 206
MunI CAATTG 2 cut(s) 39, 435
Mva1269I GAATGC 1 cut(s) 31
NciI CCSGG 1 cut(s) 206
NdeI CATATG 1 cut(s) 139
NlaIII CATG 2 cut(s) 10, 488
NsiI ATGCAT 1 cut(s) 490
PaeR7I CTCGAG 1 cut(s) 468
PceI AGGCCT 1 cut(s) 150
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 200, 504
PspXI VCTCGAGB 1 cut(s) 468
PstI CTGCAG 1 cut(s) 518
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SaqAI TTAA 1 cut(s) 444
ScrFI CCNGG 1 cut(s) 206
SetI ASST 1 cut(s) 423
SfaNI GCATC 2 cut(s) 268, 497
SfcI CTRYAG 1 cut(s) 514
Sfr274I CTCGAG 1 cut(s) 468
SlaI CTCGAG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
Sse9I AATT 3 cut(s) 39, 301, 435
SseBI AGGCCT 1 cut(s) 150
SsiI CCGC 1 cut(s) 427
StuI AGGCCT 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 366
TaqI TCGA 5 cut(s) 189, 198, 261, 276, 469
TasI AATT 3 cut(s) 39, 301, 435
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 2 cut(s) 200, 504
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 2 cut(s) 369, 384
TspDTI ATGAA 2 cut(s) 156, 335
TspRI CASTG 2 cut(s) 369, 384
XhoI CTCGAG 1 cut(s) 468
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.