FvH4_1g00520

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000805
Physical Location & Seq
Forward (+)
282389 .. 283945
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00520.t1

Sequence Viewer

Length: 936 bp
ATGGGTAGTATTCCTAACACAGAGGCTAAGATTCCGGTCATAGACTTCTCCGGCGACGACTTAAAGCCCGGATCAAGTTCATGGCTGGAAGCCTGCAACAAAGTCTGCAACGCACTCGAAGACTACGGTTGCTTTGTGGCAACATTGTCAGATGCAGACTGTCTAGAGCTTCACAACAGCATCTTTGGTAGCTTGGACGACTTGTTTGATTTTCCCAAAGAGATCAAGCTCCAGAACACTTATCAGAAGCCTTTCCGTGGTTACCATTCACCTAACTCTATTCATGAAGGCTTGGGGATCGATGATCCCACAGACCCTGAGGCTACTCATAAGTTCACCAAACTCTTCTGGCCCGATGGAAATCATCGTTTCTGTGAGACGGCAAATTCATATGCCAAGCTGGTGACGGGGCTAGACCAGACTGTGACAAGAATGATATTTGATCACTATGGTGTGTACAAGTACTGTGAATCTCATATTGGTTCCACTGATTATGTTCTTCGACTTCACAAATACAATGCAGCTGACAAGGAGAAAACCCACAAAGCTCTTCCGGAGCACACAGACTTGAACCTCACCACCATTATTCATCAAAACCATGTAAATGGCTTGGAGGTCAAAACAAAGCATGGTGATCAGTGGATTGGCTTCGATGCCTCCCCTAACTCTTTTATCTTCATGACTGGGGACGGGTTTCAGGTATGGAGCAATGAAAGAATTCAACCTTGTCTGCATAGAGTGACGATGAGCGAAAACGCAGTCAGATACTCAGTTCTGCTCTCTACATTCCACAATGGGATCGTAACTGTGCCCGAGGAGTTGATCGATGACGAACACCCTTTGAAATATAAGCCACTGAATCATCTTGAATACCTTGCTTCACAACTGGCTCAACCTACAAGCAGGGTTTGTGTTAAGAACTTCTGTGGTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

312

Amino Acids

35.16

Weight (kDa)

5.58

Isoelectric Point (pI)

29.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 11 - 92 5.2e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 164 - 258 1.7e-17 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 553
AclWI GGATC 4 cut(s) 79, 299, 305, 806
AcsI RAATTY 2 cut(s) 385, 717
AfaI GTAC 2 cut(s) 458, 464
AfiI CCNNNNNNNGG 1 cut(s) 257
AgsI TTSAA 4 cut(s) 571, 722, 844, 869
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AleI CACNNNNGTG 1 cut(s) 450
AluBI AGCT 6 cut(s) 169, 192, 229, 400, 524, 548
AluI AGCT 6 cut(s) 169, 192, 229, 400, 524, 548
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 4 cut(s) 79, 299, 305, 806
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 812
Aor13HI TCCGGA 1 cut(s) 553
AoxI GGCC 1 cut(s) 350
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 2 cut(s) 385, 717
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 744, 776
Asp700I GAANNNNTTC 2 cut(s) 251, 717
AspS9I GGNCC 1 cut(s) 351
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 5 cut(s) 261, 328, 415, 568, 644
AvaI CYCGRG 1 cut(s) 812
AxyI CCTNAGG 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 813
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 533
BccI CCATC 1 cut(s) 350
BceAI ACGGC 1 cut(s) 396
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BclI TGATCA 2 cut(s) 442, 634
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 164, 413
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 1 cut(s) 69
BmeT110I CYCGRG 1 cut(s) 812
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 1 cut(s) 69
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 3 cut(s) 142, 189, 643
BmuI ACTGGG 1 cut(s) 693
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 69
Bsa29I ATCGAT 2 cut(s) 300, 825
BsaBI GATNNNNATC 1 cut(s) 360
BsaJI CCNNGG 2 cut(s) 256, 813
BsaWI WCCGGW 2 cut(s) 34, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 688, 891
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 715
Bse8I GATNNNNATC 1 cut(s) 360
BseAI TCCGGA 1 cut(s) 553
BseCI ATCGAT 2 cut(s) 300, 825
BseDI CCNNGG 2 cut(s) 256, 813
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 2 cut(s) 309, 783
BseNI ACTGG 2 cut(s) 688, 891
BseRI GAGGAG 1 cut(s) 830
BseSI GKGCMC 1 cut(s) 813
BseXI GCAGC 1 cut(s) 533
BshFI GGCC 1 cut(s) 352
BshVI ATCGAT 2 cut(s) 300, 825
BsiHKAI GWGCWC 1 cut(s) 561
BsiHKCI CYCGRG 1 cut(s) 812
BsiSI CCGG 4 cut(s) 35, 51, 69, 554
BslFI GGGAC 1 cut(s) 701
BslI CCNNNNNNNGG 1 cut(s) 257
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 701
BsnI GGCC 1 cut(s) 352
BsoBI CYCGRG 1 cut(s) 812
Bsp1286I GDGCHC 2 cut(s) 561, 813
Bsp13I TCCGGA 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
BspANI GGCC 1 cut(s) 352
BspCNI CTCAG 2 cut(s) 310, 782
BspDI ATCGAT 2 cut(s) 300, 825
BspEI TCCGGA 1 cut(s) 553
BspHI TCATGA 2 cut(s) 283, 678
BspLI GGNNCC 1 cut(s) 484
BspPI GGATC 4 cut(s) 79, 299, 305, 806
BspQI GCTCTTC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 715
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 688, 891
BssECI CCNNGG 2 cut(s) 256, 813
BssMI GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
Bst4CI ACNGT 5 cut(s) 128, 161, 424, 467, 808
Bst6I CTCTTC 2 cut(s) 350, 555
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 3 cut(s) 27, 318, 769
BstDSI CCRYGG 1 cut(s) 256
BstEII GGTNACC 1 cut(s) 260
BstKTI GATC 8 cut(s) 74, 225, 300, 307, 445, 637, 801, 825
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 813
BstV1I GCAGC 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 605
Bsu15I ATCGAT 2 cut(s) 300, 825
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 1 cut(s) 352
BsuTUI ATCGAT 2 cut(s) 300, 825
BtgI CCRYGG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 486, 644, 854
Cac8I GCNNGC 1 cut(s) 94
CaiI CAGNNNCTG 1 cut(s) 317
CciI TCATGA 2 cut(s) 283, 678
Cfr13I GGNCC 1 cut(s) 351
ClaI ATCGAT 2 cut(s) 300, 825
Csp6I GTAC 2 cut(s) 457, 463
CviAII CATG 5 cut(s) 81, 284, 599, 629, 679
CviQI GTAC 2 cut(s) 457, 463
DdeI CTNAG 3 cut(s) 27, 318, 769
DpnI GATC 8 cut(s) 73, 224, 299, 306, 444, 636, 800, 824
DpnII GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
Eam1104I CTCTTC 2 cut(s) 350, 555
EarI CTCTTC 2 cut(s) 350, 555
Eco81I CCTNAGG 1 cut(s) 318
Eco88I CYCGRG 1 cut(s) 812
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 717
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 5 cut(s) 84, 287, 602, 632, 682
FaqI GGGAC 1 cut(s) 701
FatI CATG 5 cut(s) 80, 283, 598, 628, 678
FauNDI CATATG 1 cut(s) 391
FbaI TGATCA 2 cut(s) 442, 634
Fnu4HI GCNGC 1 cut(s) 522
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 2 cut(s) 164, 413
GluI GCNGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 1 cut(s) 352
HapII CCGG 4 cut(s) 35, 51, 69, 554
Hin1II CATG 5 cut(s) 84, 287, 602, 632, 682
HinfI GANTC 3 cut(s) 31, 470, 859
HpaII CCGG 4 cut(s) 35, 51, 69, 554
HphI GGTGA 5 cut(s) 261, 328, 415, 568, 644
Hpy166II GTNNAC 2 cut(s) 336, 457
Hpy188I TCNGA 3 cut(s) 151, 246, 764
Hpy188III TCNNGA 6 cut(s) 164, 232, 284, 554, 679, 866
Hpy8I GTNNAC 2 cut(s) 336, 457
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 5 cut(s) 128, 161, 424, 467, 808
HpyCH4V TGCA 5 cut(s) 96, 108, 155, 521, 733
HpyF3I CTNAG 3 cut(s) 27, 318, 769
Hsp92II CATG 5 cut(s) 84, 287, 602, 632, 682
Kpn2I TCCGGA 1 cut(s) 553
Ksp22I TGATCA 2 cut(s) 442, 634
Kzo9I GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 3 cut(s) 234, 556, 705
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 3 cut(s) 142, 189, 643
MaeI CTAG 2 cut(s) 164, 413
MaeIII GTNAC 5 cut(s) 260, 403, 424, 739, 802
MalI GATC 8 cut(s) 73, 224, 299, 306, 444, 636, 800, 824
MboI GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
MboII GAAGA 5 cut(s) 131, 337, 491, 542, 667
MhlI GDGCHC 2 cut(s) 561, 813
MluCI AATT 2 cut(s) 385, 717
MnlI CCTC 6 cut(s) 16, 313, 584, 607, 667, 808
MroI TCCGGA 1 cut(s) 553
MroXI GAANNNNTTC 2 cut(s) 251, 717
MseI TTAA 2 cut(s) 62, 915
MslI CAYNNNNRTG 2 cut(s) 450, 603
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 4 cut(s) 35, 51, 69, 554
MspR9I CCNGG 1 cut(s) 69
NciI CCSGG 1 cut(s) 69
NdeI CATATG 1 cut(s) 391
NdeII GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
NlaIII CATG 5 cut(s) 84, 287, 602, 632, 682
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 3 cut(s) 403, 424, 739
OliI CACNNNNGTG 1 cut(s) 450
PagI TCATGA 2 cut(s) 283, 678
PciSI GCTCTTC 1 cut(s) 555
PdmI GAANNNNTTC 2 cut(s) 251, 717
PfeI GAWTC 3 cut(s) 31, 470, 859
PkrI GCNGC 1 cut(s) 523
PspEI GGTNACC 1 cut(s) 260
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 1 cut(s) 351
PstNI CAGNNNCTG 1 cut(s) 317
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 2 cut(s) 458, 464
RsaNI GTAC 2 cut(s) 457, 463
RseI CAYNNNNRTG 2 cut(s) 450, 603
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 2 cut(s) 62, 915
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 8 cut(s) 71, 222, 297, 304, 442, 634, 798, 822
Sau96I GGNCC 1 cut(s) 351
ScaI AGTACT 1 cut(s) 464
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 2 cut(s) 561, 813
SfaNI GCATC 3 cut(s) 142, 189, 643
SmiMI CAYNNNNRTG 2 cut(s) 450, 603
Sse9I AATT 2 cut(s) 385, 717
SspMI CTAG 2 cut(s) 164, 413
StyD4I CCNGG 1 cut(s) 67
TaaI ACNGT 5 cut(s) 128, 161, 424, 467, 808
TaqI TCGA 5 cut(s) 117, 300, 502, 651, 825
TasI AATT 2 cut(s) 385, 717
TatI WGTACW 2 cut(s) 456, 462
TfiI GAWTC 3 cut(s) 31, 470, 859
Tru1I TTAA 2 cut(s) 62, 915
Tru9I TTAA 2 cut(s) 62, 915
TscAI CASTG 3 cut(s) 493, 644, 861
TseFI GTSAC 3 cut(s) 403, 424, 739
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 3 cut(s) 403, 424, 739
TspDTI ATGAA 7 cut(s) 69, 272, 300, 378, 578, 667, 726
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 3 cut(s) 493, 644, 861
XapI RAATTY 2 cut(s) 385, 717
XbaI TCTAGA 1 cut(s) 163
XmnI GAANNNNTTC 2 cut(s) 251, 717
XspI CTAG 2 cut(s) 164, 413
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.