MD06G1041300.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000805
Physical Location & Seq
Reverse (-)
5419888 .. 5421312
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1041300.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCCAAACTAGCAATAGTAGATTTCTCCAATGAAGACTGCTTCAAGCCTGGGACAAGTTCTTGGCTCTCAATACGCAAAGACATTTGCCATGCACTCGAGGAGCTTGGCTGTTTCGTGGCAATATTACCCAACAAAATTCCTTCGGAGGTTCGCAGTACTTTCTTCGACACGTTGGACGAGTTGTTTGATTTCCCAACTGAAATGAAAGTGAAAAGTTCTTATGAAAAGCCTTACCCCACTGGCTACATAAAAGTTAAGACCGCAGCTTTTGAAAGTTTGGGGATTGCTGGTGCTACAAACCCTGAACAAACTCAGATATTCGAACATCATTTTTGGCCTAATGGAAATGATGTATTTCGTGAGAGTGCCGATTTGTATACAAAAGTGATGGAAGGACTACATCATGCTGTCACGAGAATGGTGTTTGAAAACTATGGTGTAGAGGAGTACCATGATGATCACCTTCAATTGACTGTTCACACGTGCCGATTTAACAAATACTGTGAGCCTGTGAAAACTGGAACTAATGTGGGTCTGCCCGGTCATACAGATAAAAACTTTAGCACCATACTCTATCAAAATCATGTCAAAGGTCTGGAAATATGTTCAAAGGACGATGAGTGGATATGTTTTGATCCTCTGCCTTCGTCCTTCATATTTCTAGCAGGTGATGCATTTCAGGTTTGGAGCAACGACAGAATACGAGCTTGTAAACATCGAGTGACCTTGAGCGTGAATGATGTACGACACTCACTCGGACTATTTTCATTCAGGGTAGGGGAAACACATATACAGAAGGAGCTTATAGACGAGGACCACCCCCTACAGTATAAGCCATTGAATAACTTGGAATATATTCAATATTTCAAGAAAAATGGCTCCAGCCTTGGACCTGACTATACCGTCAAGGCATATTGTGGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

310

Amino Acids

35.34

Weight (kDa)

5.68

Isoelectric Point (pI)

22.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 6 - 113 1.4e-06 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 163 - 256 8.8e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 659
AasI GACNNNNNNGTC 1 cut(s) 905
Acc36I ACCTGC 1 cut(s) 659
AccI GTMKAC 1 cut(s) 380
AciI CCGC 1 cut(s) 264
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 138
AcvI CACGTG 1 cut(s) 486
AfaI GTAC 3 cut(s) 160, 452, 747
AflIII ACRYGT 2 cut(s) 171, 483
AgsI TTSAA 8 cut(s) 46, 275, 431, 470, 612, 844, 863, 871
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 4 cut(s) 106, 269, 710, 805
AluI AGCT 4 cut(s) 106, 269, 710, 805
AlwI GGATC 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 2 cut(s) 3, 338
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 138
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
Asp700I GAANNNNTTC 1 cut(s) 858
AspS9I GGNCC 2 cut(s) 817, 893
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 455, 683
AsuII TTCGAA 1 cut(s) 324
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 2 cut(s) 817, 893
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 415
BbrPI CACGTG 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 278
BccI CCATC 1 cut(s) 385
BciT130I CCWGG 1 cut(s) 51
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 543
BfaI CTAG 2 cut(s) 11, 665
BfmI CTRYAG 1 cut(s) 827
BfuAI ACCTGC 1 cut(s) 659
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
BmcAI AGTACT 1 cut(s) 160
Bme1390I CCNGG 2 cut(s) 51, 543
Bme18I GGWCC 2 cut(s) 817, 893
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 2 cut(s) 817, 893
BmiI GGNNCC 1 cut(s) 883
BmrFI CCNGG 2 cut(s) 51, 543
BmsI GCATC 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 868
Bpu14I TTCGAA 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 751
BpuMI CCSGG 1 cut(s) 543
BsaAI YACGTR 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 50, 889
Bse1I ACTGG 2 cut(s) 247, 526
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 50, 889
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 2 cut(s) 247, 526
BseRI GAGGAG 2 cut(s) 116, 461
BseXI GCAGC 1 cut(s) 278
BshFI GGCC 2 cut(s) 5, 340
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 1 cut(s) 543
BslFI GGGAC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 2 cut(s) 5, 340
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 324
Bsp143I GATC 2 cut(s) 460, 637
BspACI CCGC 1 cut(s) 264
BspANI GGCC 2 cut(s) 5, 340
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 883
BspMI ACCTGC 1 cut(s) 659
BspPI GGATC 1 cut(s) 632
BspT104I TTCGAA 1 cut(s) 324
BsrI ACTGG 2 cut(s) 247, 526
BssECI CCNNGG 2 cut(s) 50, 889
BssMI GATC 2 cut(s) 460, 637
BssNAI GTATAC 1 cut(s) 381
BssSI CACGAG 1 cut(s) 415
BssT1I CCWWGG 1 cut(s) 889
Bst1107I GTATAC 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 4 cut(s) 478, 506, 831, 907
BstAPI GCANNNNNTGC 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 486
BstBI TTCGAA 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 315
BstKTI GATC 2 cut(s) 463, 640
BstMBI GATC 2 cut(s) 460, 637
BstMWI GCNNNNNNNGC 2 cut(s) 11, 674
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 49, 541
BstSFI CTRYAG 1 cut(s) 827
BstV1I GCAGC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 42
BstZ17I GTATAC 1 cut(s) 381
BsuRI GGCC 2 cut(s) 5, 340
BtsIMutI CAGTG 1 cut(s) 240
BveI ACCTGC 1 cut(s) 659
Cfr13I GGNCC 2 cut(s) 817, 893
Csp6I GTAC 3 cut(s) 159, 451, 746
CviAII CATG 4 cut(s) 92, 407, 455, 587
CviQI GTAC 3 cut(s) 159, 451, 746
DdeI CTNAG 1 cut(s) 315
DpnI GATC 2 cut(s) 462, 639
DpnII GATC 2 cut(s) 460, 637
DrdI GACNNNNNNGTC 1 cut(s) 905
DseDI GACNNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 889
Eco47I GGWCC 2 cut(s) 817, 893
Eco72I CACGTG 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 889
EcoT22I ATGCAT 1 cut(s) 679
ErhI CCWWGG 1 cut(s) 889
FaeI CATG 4 cut(s) 95, 410, 458, 590
FaqI GGGAC 1 cut(s) 67
FatI CATG 4 cut(s) 91, 406, 454, 586
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 380
Fnu4HI GCNGC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 2 cut(s) 11, 665
GluI GCNGC 1 cut(s) 267
GsuI CTGGAG 1 cut(s) 868
HaeIII GGCC 2 cut(s) 5, 340
HapII CCGG 1 cut(s) 543
Hin1II CATG 4 cut(s) 95, 410, 458, 590
HpaII CCGG 1 cut(s) 543
HphI GGTGA 2 cut(s) 455, 683
Hpy166II GTNNAC 3 cut(s) 381, 481, 716
Hpy188I TCNGA 3 cut(s) 148, 318, 761
Hpy188III TCNNGA 4 cut(s) 362, 415, 599, 871
Hpy8I GTNNAC 3 cut(s) 381, 481, 716
HpyAV CCTTC 6 cut(s) 153, 389, 476, 657, 664, 793
HpyCH4III ACNGT 4 cut(s) 478, 506, 831, 907
HpyCH4IV ACGT 2 cut(s) 173, 485
HpyCH4V TGCA 2 cut(s) 95, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 674
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 2 cut(s) 173, 485
Hsp92II CATG 4 cut(s) 95, 410, 458, 590
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 460, 637
LmnI GCTCC 4 cut(s) 103, 690, 802, 887
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 1 cut(s) 664
MaeI CTAG 2 cut(s) 11, 665
MaeII ACGT 2 cut(s) 173, 485
MaeIII GTNAC 2 cut(s) 412, 724
MalI GATC 2 cut(s) 462, 639
MboI GATC 2 cut(s) 460, 637
MboII GAAGA 2 cut(s) 47, 157
MfeI CAATTG 1 cut(s) 470
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 138, 470
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 5 cut(s) 94, 142, 439, 651, 808
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 679
MroXI GAANNNNTTC 1 cut(s) 858
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 258, 495
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 2 cut(s) 51, 543
MunI CAATTG 1 cut(s) 470
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 11, 674
NciI CCSGG 1 cut(s) 543
NdeII GATC 2 cut(s) 460, 637
NlaIII CATG 4 cut(s) 95, 410, 458, 590
NlaIV GGNNCC 1 cut(s) 883
NmuCI GTSAC 2 cut(s) 412, 724
NsiI ATGCAT 1 cut(s) 679
NspV TTCGAA 1 cut(s) 324
PaeR7I CTCGAG 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 659
PdmI GAANNNNTTC 1 cut(s) 858
PkrI GCNGC 1 cut(s) 268
PmaCI CACGTG 1 cut(s) 486
PmlI CACGTG 1 cut(s) 486
Ppu21I YACGTR 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 49
PspCI CACGTG 1 cut(s) 486
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 883
PspPI GGNCC 2 cut(s) 817, 893
PspXI VCTCGAGB 1 cut(s) 98
RsaI GTAC 3 cut(s) 160, 452, 747
RsaNI GTAC 3 cut(s) 159, 451, 746
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 2 cut(s) 258, 495
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 2 cut(s) 460, 637
Sau96I GGNCC 2 cut(s) 817, 893
ScaI AGTACT 1 cut(s) 160
ScrFI CCNGG 2 cut(s) 51, 543
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 1 cut(s) 827
Sfr274I CTCGAG 1 cut(s) 98
SfuI TTCGAA 1 cut(s) 324
SinI GGWCC 2 cut(s) 817, 893
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 2 cut(s) 98, 730
SmoI CTYRAG 2 cut(s) 98, 730
Sse9I AATT 2 cut(s) 138, 470
SsiI CCGC 1 cut(s) 264
SspI AATATT 2 cut(s) 126, 866
SspMI CTAG 2 cut(s) 11, 665
StyD4I CCNGG 2 cut(s) 49, 541
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 4 cut(s) 478, 506, 831, 907
TaiI ACGT 2 cut(s) 176, 488
TaqI TCGA 4 cut(s) 99, 168, 324, 721
TasI AATT 2 cut(s) 138, 470
TatI WGTACW 1 cut(s) 158
Tru1I TTAA 2 cut(s) 258, 495
Tru9I TTAA 2 cut(s) 258, 495
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 2 cut(s) 412, 724
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 2 cut(s) 412, 724
TspDTI ATGAA 5 cut(s) 48, 221, 240, 646, 759
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 2 cut(s) 817, 893
XapI RAATTY 1 cut(s) 138
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 2 cut(s) 11, 665
ZrmI AGTACT 1 cut(s) 160
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.