MD06G1041500.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000805
Physical Location & Seq
Reverse (-)
5499354 .. 5500234
881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1041500.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGCCAAACTAGCAATAGTAGATTTCTCCAATGAAGACTGCTTCAAGCCTGGGACAAGTTCTTGGCTCTCAATACGCAAAGACATTTGCCATGCACTCGAGGAGCTTGGCTGTTTCGTGGCAATATTACCCAACAAAATTTCTTCGGAGCTTTGCAGTACCTTCTTCCGTATGTTGGACGAGTTGTTTGATTTCCCAACAGAAATCAAAGTGAAAAACTCTTATGAAAAGCCTTACCCCACTGGCTACCTAAACGTTGGGAACACCGGTTATGAAAGCTTGGCTATTGCTGATGCGGGTCTCCGCAGTCATAGAGACAGAGACTTTAGCACCATACTTTGTCAAAATCATGTCAAAGGTCTGGAAATATATTCAAAGGACGATGAGTGGATATGTTTTGATCCTCTGCTTTCGTCCTTCGTATTTCTAGCGGGTGATGGACTTCAGGTTTGGAGCAACGATAGAATACGAGCTTGTAAACATCAAGTGACCTTGAGCGAGAATGATGTACGGTACTCGCTCGGACTATTTTCATTCAGGGCAGGGGAAACACATACACCGAAGGAGCTTATAGACGAGGACCACCCCCTACAGTATAATCCATTGAATGACTTGGAATATATTGAATATACAAAGAAAAATTACTCCAGCCTTGGACCTGACTATACCGTCAAGGCATATTGTGGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

231

Amino Acids

26.2

Weight (kDa)

4.98

Isoelectric Point (pI)

25.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 6 - 90 2.4e-06 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 99 - 178 1.6e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 668
AciI CCGC 3 cut(s) 296, 304, 431
AclI AACGTT 1 cut(s) 255
AclWI GGATC 1 cut(s) 395
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 138
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 3 cut(s) 160, 510, 515
AfiI CCNNNNNNNGG 1 cut(s) 175
AgeI ACCGGT 1 cut(s) 266
AgsI TTSAA 4 cut(s) 46, 375, 607, 626
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 5 cut(s) 106, 151, 279, 473, 568
AluI AGCT 5 cut(s) 106, 151, 279, 473, 568
Alw26I GTCTC 3 cut(s) 305, 309, 315
AlwI GGATC 1 cut(s) 395
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 138
ArsI GACNNNNNNTTYG 4 cut(s) 170, 202, 432, 464
AsiGI ACCGGT 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 580, 656
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 2 cut(s) 580, 656
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 42
BccI CCATC 1 cut(s) 431
BciT130I CCWGG 1 cut(s) 51
BcoDI GTCTC 3 cut(s) 305, 309, 315
BfaI CTAG 2 cut(s) 11, 428
BfmI CTRYAG 1 cut(s) 590
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 2 cut(s) 580, 656
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 2 cut(s) 580, 656
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 631
BpuEI CTTGAG 1 cut(s) 514
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 50, 652
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 50, 652
BseLI CCNNNNNNNGG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 247
BseRI GAGGAG 1 cut(s) 116
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 3 cut(s) 305, 309, 315
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 98
Bsp143I GATC 1 cut(s) 400
BspACI CCGC 3 cut(s) 296, 304, 431
BspANI GGCC 1 cut(s) 5
BspPI GGATC 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 247
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 2 cut(s) 50, 652
BssMI GATC 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 652
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 513, 594, 670
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 3 cut(s) 305, 309, 315
BstMBI GATC 1 cut(s) 400
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstSFI CTRYAG 1 cut(s) 590
BstV2I GAAGAC 1 cut(s) 42
BsuRI GGCC 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 240
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 580, 656
Csp6I GTAC 3 cut(s) 159, 509, 514
CspAI ACCGGT 1 cut(s) 266
CviAII CATG 2 cut(s) 92, 350
CviQI GTAC 3 cut(s) 159, 509, 514
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
DrdI GACNNNNNNGTC 1 cut(s) 668
DseDI GACNNNNNNGTC 1 cut(s) 668
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 2 cut(s) 580, 656
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 652
ErhI CCWWGG 1 cut(s) 652
FaeI CATG 2 cut(s) 95, 353
FaqI GGGAC 1 cut(s) 67
FatI CATG 2 cut(s) 91, 349
FauI CCCGC 2 cut(s) 289, 424
FspBI CTAG 2 cut(s) 11, 428
GsuI CTGGAG 1 cut(s) 631
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 267
Hin1II CATG 2 cut(s) 95, 353
HindIII AAGCTT 1 cut(s) 277
HpaII CCGG 1 cut(s) 267
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 1 cut(s) 479
Hpy188I TCNGA 2 cut(s) 148, 524
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 1 cut(s) 479
HpyAV CCTTC 3 cut(s) 172, 427, 556
HpyCH4III ACNGT 3 cut(s) 513, 594, 670
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 2 cut(s) 95, 156
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 2 cut(s) 95, 353
Kzo9I GATC 1 cut(s) 400
LmnI GCTCC 4 cut(s) 103, 148, 453, 565
LweI GCATC 1 cut(s) 283
MaeI CTAG 2 cut(s) 11, 428
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 487
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MboII GAAGA 3 cut(s) 47, 135, 157
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 138, 640
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 3 cut(s) 94, 414, 571
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 267
MspR9I CCNGG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 1 cut(s) 400
NlaIII CATG 2 cut(s) 95, 353
NmuCI GTSAC 1 cut(s) 487
PaeR7I CTCGAG 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 266
Psp1406I AACGTT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspPI GGNCC 2 cut(s) 580, 656
PspXI VCTCGAGB 1 cut(s) 98
RsaI GTAC 3 cut(s) 160, 510, 515
RsaNI GTAC 3 cut(s) 159, 509, 514
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 2 cut(s) 580, 656
ScrFI CCNGG 1 cut(s) 51
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 1 cut(s) 590
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 2 cut(s) 580, 656
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 98, 493
SmoI CTYRAG 2 cut(s) 98, 493
Sse9I AATT 2 cut(s) 138, 640
SsiI CCGC 3 cut(s) 296, 304, 431
SspI AATATT 1 cut(s) 126
SspMI CTAG 2 cut(s) 11, 428
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 3 cut(s) 513, 594, 670
TaiI ACGT 1 cut(s) 258
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 138, 640
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 487
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 4 cut(s) 48, 240, 288, 522
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 2 cut(s) 580, 656
XapI RAATTY 1 cut(s) 138
XhoI CTCGAG 1 cut(s) 98
XspI CTAG 2 cut(s) 11, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.