Rroxscaffold_3G00250550

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000805
Physical Location & Seq
Forward (+)
44381263 .. 44382199
937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250550.1

Sequence Viewer

Length: 747 bp
ATGGAAATCAAAGTGCCAAATACTAACAAAAACGACGGCGGTGGTGGCTACTCGACTTTGCATCCTGCCCATGAAAGCTTTGGGATTGTTAATAGTACGAACCCGGAAGAAACACAGAAGTTCACAAATCGCTTTTGGACTAATGGAAACGATCAGTTTCGGTATGGTTGTTTGATTTACAGTTGTGAGAGTGTTGATTCGTATGCAAAAGTGATGGCAGAACTAGATCGAGCTGTGACAAGAATGGTATTCGAAAACTATGGTGTAGACAAGTACCACGATGATCACGTTCAATCTACTTTTCACGGTCTCCGATTTAGTAAATACAAAGAACCCAAGAAAACTGGAATTGACGTCGGTCTAGGCAGTCATACAGATAAGACCTTTACCACCATCCTCCATCAAAATAATGTGAATGGTCTAGAGATCAATACAAAGAACGACGAGTGGATATGCTGTGATTATCCTCTTCCTTCATCCTTCCTATTCCTAGCAGGCGATGTATTCCAGGTCTGGAGCAATGACAGAATACGACCTTGTAGACATAGAGCCAGCTTGACTGAGAATAACGTGAGATACTCTTTCGGACTCTATTCGATCAAGGAAGGAGTGACAAACATACCGGACGAGCTTGTGGACGAGGACCATCCCCTACGGTTTAAGCCATTGAATCATATAGAATATGCTAGAGCAAGATATGACAACAATGGGACTGACTTGGGCGTCAAGTCATATTGTGGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

248

Amino Acids

28.36

Weight (kDa)

5.92

Isoelectric Point (pI)

23.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 109 - 195 3.4e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 722
AatII GACGTC 1 cut(s) 357
AccI GTMKAC 2 cut(s) 267, 541
AciI CCGC 1 cut(s) 39
AcyI GRCGYC 2 cut(s) 354, 723
AfaI GTAC 2 cut(s) 97, 275
AgsI TTSAA 2 cut(s) 293, 670
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 4 cut(s) 78, 233, 555, 631
AluI AGCT 4 cut(s) 78, 233, 555, 631
Alw26I GTCTC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 643
AsuC2I CCSGG 1 cut(s) 104
AsuII TTCGAA 1 cut(s) 252
AvaII GGWCC 1 cut(s) 643
BccI CCATC 4 cut(s) 208, 401, 408, 654
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 509
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 5 cut(s) 224, 362, 422, 491, 687
Bme1390I CCNGG 2 cut(s) 104, 509
Bme18I GGWCC 1 cut(s) 643
BmgT120I GGNCC 1 cut(s) 643
BmrFI CCNGG 2 cut(s) 104, 509
BmsI GCATC 1 cut(s) 70
BoxI GACNNNNGTC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 535
Bpu14I TTCGAA 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 104
BsaHI GRCGYC 2 cut(s) 354, 723
BsaI GGTCTC 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 622
Bse1I ACTGG 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 526
BseBI CCWGG 1 cut(s) 509
BseGI GGATG 4 cut(s) 61, 393, 476, 646
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 1 cut(s) 349
BsiSI CCGG 2 cut(s) 104, 623
BslFI GGGAC 1 cut(s) 724
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 724
Bso31I GGTCTC 1 cut(s) 314
Bsp119I TTCGAA 1 cut(s) 252
Bsp143I GATC 5 cut(s) 151, 226, 283, 426, 597
BspACI CCGC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 553
BspT104I TTCGAA 1 cut(s) 252
BspTNI GGTCTC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 526
BsrI ACTGG 1 cut(s) 349
BssMI GATC 5 cut(s) 151, 226, 283, 426, 597
BssNI GRCGYC 2 cut(s) 354, 723
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 182, 308, 657
Bst6I CTCTTC 1 cut(s) 474
BstACI GRCGYC 2 cut(s) 354, 723
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 496, 553
BstDEI CTNAG 1 cut(s) 561
BstF5I GGATG 4 cut(s) 61, 393, 476, 646
BstKTI GATC 5 cut(s) 154, 229, 286, 429, 600
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 5 cut(s) 151, 226, 283, 426, 597
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNI CCWGG 1 cut(s) 509
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 102, 507
BtgZI GCGATG 1 cut(s) 513
BtsCI GGATG 4 cut(s) 61, 393, 476, 646
Cac8I GCNNGC 2 cut(s) 496, 553
Cfr13I GGNCC 1 cut(s) 643
CseI GACGC 1 cut(s) 712
Csp6I GTAC 2 cut(s) 96, 274
CviAII CATG 1 cut(s) 71
CviJI RGCY 7 cut(s) 48, 78, 233, 551, 555, 631, 664
CviKI_1 RGCY 7 cut(s) 48, 78, 233, 551, 555, 631, 664
CviQI GTAC 2 cut(s) 96, 274
DdeI CTNAG 1 cut(s) 561
DpnI GATC 5 cut(s) 153, 228, 285, 428, 599
DpnII GATC 5 cut(s) 151, 226, 283, 426, 597
DrdI GACNNNNNNGTC 1 cut(s) 722
DseDI GACNNNNNNGTC 1 cut(s) 722
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 507
FaeI CATG 1 cut(s) 74
FaqI GGGAC 1 cut(s) 724
FatI CATG 1 cut(s) 70
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 2 cut(s) 267, 541
FokI GGATG 4 cut(s) 48, 380, 463, 633
FspBI CTAG 5 cut(s) 224, 362, 422, 491, 687
GsuI CTGGAG 1 cut(s) 535
HapII CCGG 2 cut(s) 104, 623
HgaI GACGC 1 cut(s) 712
Hin1I GRCGYC 2 cut(s) 354, 723
Hin1II CATG 1 cut(s) 74
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 3 cut(s) 197, 588, 670
HpaII CCGG 2 cut(s) 104, 623
Hpy166II GTNNAC 4 cut(s) 123, 268, 542, 637
Hpy188I TCNGA 2 cut(s) 314, 587
Hpy188III TCNNGA 2 cut(s) 422, 514
Hpy8I GTNNAC 4 cut(s) 123, 268, 542, 637
Hpy99I CGWCG 3 cut(s) 38, 359, 446
HpyAV CCTTC 3 cut(s) 483, 490, 599
HpyCH4III ACNGT 3 cut(s) 182, 308, 657
HpyCH4IV ACGT 3 cut(s) 288, 354, 570
HpyCH4V TGCA 2 cut(s) 61, 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 3 cut(s) 288, 354, 570
Hsp92I GRCGYC 2 cut(s) 354, 723
Hsp92II CATG 1 cut(s) 74
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 5 cut(s) 151, 226, 283, 426, 597
LmnI GCTCC 1 cut(s) 516
LpnPI CCDG 9 cut(s) 78, 117, 330, 480, 494, 499, 521, 565, 636
LweI GCATC 1 cut(s) 70
MaeI CTAG 5 cut(s) 224, 362, 422, 491, 687
MaeII ACGT 3 cut(s) 288, 354, 570
MaeIII GTNAC 2 cut(s) 235, 610
MalI GATC 5 cut(s) 153, 228, 285, 428, 599
MboI GATC 5 cut(s) 151, 226, 283, 426, 597
MboII GAAGA 2 cut(s) 119, 461
MluCI AATT 1 cut(s) 348
MlyI GAGTC 1 cut(s) 582
MnlI CCTC 3 cut(s) 407, 477, 634
MseI TTAA 2 cut(s) 90, 660
MspI CCGG 2 cut(s) 104, 623
MspR9I CCNGG 2 cut(s) 104, 509
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 1 cut(s) 45
NciI CCSGG 1 cut(s) 104
NdeII GATC 5 cut(s) 151, 226, 283, 426, 597
NlaIII CATG 1 cut(s) 74
NmuCI GTSAC 2 cut(s) 235, 610
NspV TTCGAA 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 2 cut(s) 197, 670
PleI GAGTC 1 cut(s) 582
PpsI GAGTC 1 cut(s) 582
PshAI GACNNNNGTC 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspPI GGNCC 1 cut(s) 643
RsaI GTAC 2 cut(s) 97, 275
RsaNI GTAC 2 cut(s) 96, 274
SaqAI TTAA 2 cut(s) 90, 660
Sau3AI GATC 5 cut(s) 151, 226, 283, 426, 597
Sau96I GGNCC 1 cut(s) 643
SchI GAGTC 1 cut(s) 582
ScrFI CCNGG 2 cut(s) 104, 509
SfaNI GCATC 1 cut(s) 70
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 1 cut(s) 643
Sse9I AATT 1 cut(s) 348
SsiI CCGC 1 cut(s) 39
SspMI CTAG 5 cut(s) 224, 362, 422, 491, 687
StyD4I CCNGG 2 cut(s) 102, 507
TaaI ACNGT 3 cut(s) 182, 308, 657
TaiI ACGT 3 cut(s) 291, 357, 573
TaqI TCGA 4 cut(s) 53, 229, 252, 596
TaqII GACCGA 1 cut(s) 347
TasI AATT 1 cut(s) 348
TfiI GAWTC 2 cut(s) 197, 670
Tru1I TTAA 2 cut(s) 90, 660
Tru9I TTAA 2 cut(s) 90, 660
TseFI GTSAC 2 cut(s) 235, 610
Tsp45I GTSAC 2 cut(s) 235, 610
TspDTI ATGAA 2 cut(s) 87, 465
VpaK11BI GGWCC 1 cut(s) 643
XbaI TCTAGA 1 cut(s) 421
XcmI CCANNNNNNNNNTGG 1 cut(s) 77
XmiI GTMKAC 2 cut(s) 267, 541
XspI CTAG 5 cut(s) 224, 362, 422, 491, 687
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.