Rroxscaffold_2G00155610

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000805
Physical Location & Seq
Reverse (-)
92010055 .. 92011668
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155610.1

Sequence Viewer

Length: 702 bp
ATGAGTTATGAGAAGCCTTTCCGTGGTTACCATTCACCTAACTCTGTTCATGAAGGCTTGGGGATCGATGATCCTACAAACCCTGAGGCTACTCATAAGTTCAGCAAACTCTTCTGGCCCCATGGAAATCATCATTTCTGTGAGACTGCAAATTCATATGCCAAGCTGGTGACGGGGCTAGACCAGACTGTGACAAGAATGATATTTGAGCACTATGGGGTGGAGAAGTACTGTGAGTCTCATGTTGGATCAACTGATTATGTTCTTAGACTCCACAAATACAATGCAGCAGACAAGGAGAAAACCCACAAAGCTCTTCCGGAGCACACAGACTTGAACCTCACTACCATCATTCATCAAAACCATGTGAATGGCTTGGAGGTCAAGACAAAGCATGGTGATCAATGGATTGGCTTTGATGCCTCCCCTAACTCTTTTATATTCATGACTGGGGACGGGTTTCAGGTATGGAGCAATGACAGAATTCAACCTTGTCTGCATAGAGTGACGATGAGCGAAAATGCAGTGAGATACTCGGTTCTACTCTCTACATTCCACAATGGGATCATAACTGTGCCCGAGGAGTTGATCGATGATGAACACCCTTTGAAATATAAGCCGCTGAATCATCTTGAATACCTTGCTTCACAACTTGCTCTAACTACCAGCAGGGTTTGTGTTAAGAATTTCTGTGGTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

233

Amino Acids

26.61

Weight (kDa)

6.29

Isoelectric Point (pI)

23.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 86 - 180 5.9e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 1 cut(s) 620
AclWI GGATC 4 cut(s) 65, 71, 256, 572
AcsI RAATTY 3 cut(s) 151, 483, 685
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 23
AgsI TTSAA 4 cut(s) 337, 488, 610, 635
AluBI AGCT 2 cut(s) 166, 314
AluI AGCT 2 cut(s) 166, 314
Alw21I GWGCWC 2 cut(s) 213, 327
Alw26I GTCTC 2 cut(s) 137, 243
AlwI GGATC 4 cut(s) 65, 71, 256, 572
Ama87I CYCGRG 1 cut(s) 578
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 3 cut(s) 151, 483, 685
Asp700I GAANNNNTTC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 27, 181, 410
AvaI CYCGRG 1 cut(s) 578
AxyI CCTNAGG 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 579
Bbv12I GWGCWC 2 cut(s) 213, 327
BbvI GCAGC 1 cut(s) 299
BccI CCATC 1 cut(s) 356
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 137, 243
BfaI CTAG 1 cut(s) 179
BisI GCNGC 2 cut(s) 288, 620
BlsI GCNGC 2 cut(s) 289, 621
BmcAI AGTACT 1 cut(s) 230
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 1 cut(s) 117
BmiI GGNNCC 1 cut(s) 119
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 1 cut(s) 409
BmuI ACTGGG 1 cut(s) 459
Bsa29I ATCGAT 2 cut(s) 66, 591
BsaJI CCNNGG 3 cut(s) 22, 121, 579
BsaWI WCCGGW 1 cut(s) 319
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 454
Bse21I CCTNAGG 1 cut(s) 84
Bse3DI GCAATG 1 cut(s) 481
BseAI TCCGGA 1 cut(s) 319
BseCI ATCGAT 2 cut(s) 66, 591
BseDI CCNNGG 3 cut(s) 22, 121, 579
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMI GCAATG 1 cut(s) 481
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 596
BseSI GKGCMC 1 cut(s) 579
BseXI GCAGC 1 cut(s) 299
BshFI GGCC 1 cut(s) 118
BshVI ATCGAT 2 cut(s) 66, 591
BsiHKAI GWGCWC 2 cut(s) 213, 327
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 23
BsmAI GTCTC 2 cut(s) 137, 243
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 1 cut(s) 118
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 3 cut(s) 213, 327, 579
Bsp13I TCCGGA 1 cut(s) 319
Bsp143I GATC 6 cut(s) 63, 70, 248, 400, 564, 588
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 1 cut(s) 620
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 1 cut(s) 76
BspDI ATCGAT 2 cut(s) 66, 591
BspEI TCCGGA 1 cut(s) 319
BspHI TCATGA 2 cut(s) 49, 444
BspLI GGNNCC 1 cut(s) 119
BspPI GGATC 4 cut(s) 65, 71, 256, 572
BspQI GCTCTTC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 481
BsrI ACTGG 1 cut(s) 454
BssECI CCNNGG 3 cut(s) 22, 121, 579
BssMI GATC 6 cut(s) 63, 70, 248, 400, 564, 588
BssT1I CCWWGG 1 cut(s) 121
Bst4CI ACNGT 3 cut(s) 190, 233, 574
Bst6I CTCTTC 2 cut(s) 116, 321
BstDEI CTNAG 2 cut(s) 84, 266
BstDSI CCRYGG 2 cut(s) 22, 121
BstEII GGTNACC 1 cut(s) 26
BstKTI GATC 6 cut(s) 66, 73, 251, 403, 567, 591
BstMAI GTCTC 2 cut(s) 137, 243
BstMBI GATC 6 cut(s) 63, 70, 248, 400, 564, 588
BstPI GGTNACC 1 cut(s) 26
BstSLI GKGCMC 1 cut(s) 579
BstV1I GCAGC 1 cut(s) 299
BstXI CCANNNNNNTGG 1 cut(s) 371
Bsu15I ATCGAT 2 cut(s) 66, 591
Bsu36I CCTNAGG 1 cut(s) 84
BsuRI GGCC 1 cut(s) 118
BsuTUI ATCGAT 2 cut(s) 66, 591
BtgI CCRYGG 2 cut(s) 22, 121
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 531
CciI TCATGA 2 cut(s) 49, 444
Cfr13I GGNCC 1 cut(s) 117
ClaI ATCGAT 2 cut(s) 66, 591
Csp6I GTAC 1 cut(s) 229
CviAII CATG 6 cut(s) 50, 122, 242, 365, 395, 445
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 2 cut(s) 84, 266
DpnI GATC 6 cut(s) 65, 72, 250, 402, 566, 590
DpnII GATC 6 cut(s) 63, 70, 248, 400, 564, 588
Eam1104I CTCTTC 2 cut(s) 116, 321
EarI CTCTTC 2 cut(s) 116, 321
Eco130I CCWWGG 1 cut(s) 121
Eco81I CCTNAGG 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 578
Eco91I GGTNACC 1 cut(s) 26
EcoO65I GGTNACC 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 6 cut(s) 53, 125, 245, 368, 398, 448
FaqI GGGAC 1 cut(s) 467
FatI CATG 6 cut(s) 49, 121, 241, 364, 394, 444
FauNDI CATATG 1 cut(s) 157
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 2 cut(s) 288, 620
Fsp4HI GCNGC 2 cut(s) 288, 620
FspBI CTAG 1 cut(s) 179
GluI GCNGC 2 cut(s) 288, 620
HaeIII GGCC 1 cut(s) 118
HapII CCGG 1 cut(s) 320
Hin1II CATG 6 cut(s) 53, 125, 245, 368, 398, 448
HinfI GANTC 3 cut(s) 236, 270, 625
HpaII CCGG 1 cut(s) 320
HphI GGTGA 3 cut(s) 27, 181, 410
Hpy188III TCNNGA 5 cut(s) 50, 320, 385, 445, 632
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 3 cut(s) 190, 233, 574
HpyCH4V TGCA 4 cut(s) 149, 287, 499, 524
HpyF3I CTNAG 2 cut(s) 84, 266
Hsp92II CATG 6 cut(s) 53, 125, 245, 368, 398, 448
Kpn2I TCCGGA 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 6 cut(s) 63, 70, 248, 400, 564, 588
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 322, 471
LpnPI CCDG 9 cut(s) 96, 100, 152, 197, 333, 435, 449, 655, 679
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 1 cut(s) 409
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 4 cut(s) 26, 169, 190, 505
MalI GATC 6 cut(s) 65, 72, 250, 402, 566, 590
MboI GATC 6 cut(s) 63, 70, 248, 400, 564, 588
MboII GAAGA 2 cut(s) 103, 308
MhlI GDGCHC 3 cut(s) 213, 327, 579
MluCI AATT 3 cut(s) 151, 483, 685
MlyI GAGTC 2 cut(s) 245, 264
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 5 cut(s) 79, 350, 373, 433, 574
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 3 cut(s) 138, 369, 572
MspA1I CMGCKG 1 cut(s) 622
MspI CCGG 1 cut(s) 320
NcoI CCATGG 1 cut(s) 121
NdeI CATATG 1 cut(s) 157
NdeII GATC 6 cut(s) 63, 70, 248, 400, 564, 588
NlaIII CATG 6 cut(s) 53, 125, 245, 368, 398, 448
NlaIV GGNNCC 1 cut(s) 119
NmuCI GTSAC 3 cut(s) 169, 190, 505
PagI TCATGA 2 cut(s) 49, 444
PciSI GCTCTTC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 625
PkrI GCNGC 2 cut(s) 289, 621
PleI GAGTC 2 cut(s) 244, 264
PpsI GAGTC 2 cut(s) 244, 264
PspEI GGTNACC 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 119
PspPI GGNCC 1 cut(s) 117
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 3 cut(s) 138, 369, 572
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 2 cut(s) 288, 620
Sau3AI GATC 6 cut(s) 63, 70, 248, 400, 564, 588
Sau96I GGNCC 1 cut(s) 117
ScaI AGTACT 1 cut(s) 230
SchI GAGTC 2 cut(s) 245, 264
SduI GDGCHC 3 cut(s) 213, 327, 579
SetI ASST 8 cut(s) 40, 168, 316, 342, 384, 468, 493, 642
SfaNI GCATC 1 cut(s) 409
SmiMI CAYNNNNRTG 3 cut(s) 138, 369, 572
Sse9I AATT 3 cut(s) 151, 483, 685
SsiI CCGC 1 cut(s) 620
SspMI CTAG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 3 cut(s) 190, 233, 574
TaqI TCGA 2 cut(s) 66, 591
TasI AATT 3 cut(s) 151, 483, 685
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 622
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 1 cut(s) 531
TseFI GTSAC 3 cut(s) 169, 190, 505
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 3 cut(s) 169, 190, 505
TspDTI ATGAA 6 cut(s) 38, 66, 144, 344, 433, 612
TspGWI ACGGA 1 cut(s) 11
TspRI CASTG 1 cut(s) 531
XapI RAATTY 3 cut(s) 151, 483, 685
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 179
ZrmI AGTACT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.