MD06G1027800.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000805
Physical Location & Seq
Forward (+)
3379109 .. 3380421
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1027800.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGCCAAAATTCCAGTGGTAGATTTCTCGAAAGATGATTGCTTGAAGCCGGGGACAAGTTCTTGGCTCTCGGCACGCAGAGATGTCTGCCGTGCACTGGAAGATATCGGCTGTTTCACGGCAATTCTTCCCAACAAAGTTCCAAAGGAACATGCCAACACCTTCTTCTCTACATTTGACGATTTGTTTGATTTTCCAACCAGTACTCCTGAAAAGCCCTTCTTCTATGCAACTTCTAATTCCGGGCAACGAACCTACGGGATCATCAACGGCACAAACCCTCAAGATGTTCAACAATTCACACATCTCATTTGGCCCGATGGAAACGATCAATTTCGTGAGAGTGCTGACTTTTATGCAAAAGTGCTGGCGGAATTAGATCAAACTGTGACAAGAATGGTGTTTGAAAACTACGGTGTAGAGAAGTACCACGACGATCACAAACTTGATAGTGATGTGGGTTTAAAGATTCATACGGACAAGAACTTCTCCACCATAATCCATCAAAACCATATCAACGGTCTGGAGATAAATACAAAGGATGATGAGTGGGTGCTTTTTGATCCTCCACCTTCATCCATTATATTCATAGCAGCTGACGGATTTCAGGTTTGGAGCAATGACAGAATCCGACCTTGTAGACATAGAGTGACCTTGACTGAAAATGTTGTAAGATACACATTTGGTGTCTTCTCTGTCAAGAAAGGGGTGATACACGTACCGAATGAGCTCGTGGACGAAGACCACCCGTTACTGTATAAGCCTTTGAATCATGTTGAGTTCCTCATGGCACAGAGACATACTGGTGAAACTGAGTATGCCGCAAAGGCATATTGTGCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

282

Amino Acids

32.14

Weight (kDa)

5.41

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 135 - 231 6.5e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 640
AciI CCGC 2 cut(s) 371, 822
AclWI GGATC 2 cut(s) 269, 557
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 9
AfaI GTAC 3 cut(s) 205, 428, 720
AfiI CCNNNNNNNGG 1 cut(s) 97
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 4 cut(s) 46, 293, 407, 769
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 2 cut(s) 596, 730
AluI AGCT 2 cut(s) 596, 730
Alw21I GWGCWC 2 cut(s) 97, 732
Alw26I GTCTC 1 cut(s) 790
Alw44I GTGCAC 1 cut(s) 93
AlwI GGATC 2 cut(s) 269, 557
AoxI GGCC 2 cut(s) 3, 314
ApaLI GTGCAC 1 cut(s) 93
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 1 cut(s) 9
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspS9I GGNCC 1 cut(s) 315
AsuC2I CCSGG 2 cut(s) 51, 244
AsuHPI GGTGA 2 cut(s) 721, 818
BaeGI GKGCMC 1 cut(s) 97
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 732
BauI CACGAG 1 cut(s) 731
BbsI GAAGAC 2 cut(s) 682, 747
Bbv12I GWGCWC 2 cut(s) 97, 732
BbvI GCAGC 1 cut(s) 605
BccI CCATC 2 cut(s) 314, 510
BceAI ACGGC 3 cut(s) 75, 135, 286
BcnI CCSGG 2 cut(s) 51, 244
BcoDI GTCTC 1 cut(s) 790
BglI GCCNNNNNGGC 1 cut(s) 827
BisI GCNGC 2 cut(s) 594, 822
BlsI GCNGC 2 cut(s) 595, 823
BmcAI AGTACT 1 cut(s) 205
Bme1390I CCNGG 2 cut(s) 51, 244
BmgT120I GGNCC 1 cut(s) 315
BmrFI CCNGG 2 cut(s) 51, 244
BpiI GAAGAC 2 cut(s) 682, 747
BpmI CTGGAG 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 267
BpuMI CCSGG 2 cut(s) 51, 244
BsaAI YACGTR 1 cut(s) 718
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 4 cut(s) 14, 102, 201, 808
Bse3DI GCAATG 1 cut(s) 625
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 2 cut(s) 547, 575
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 4 cut(s) 14, 102, 201, 808
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 1 cut(s) 605
BshFI GGCC 2 cut(s) 5, 316
BsiHKAI GWGCWC 2 cut(s) 97, 732
BsiSI CCGG 2 cut(s) 50, 243
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 790
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 2 cut(s) 5, 316
Bsp1286I GDGCHC 2 cut(s) 97, 732
Bsp143I GATC 5 cut(s) 261, 328, 379, 436, 562
BspACI CCGC 2 cut(s) 371, 822
BspANI GGCC 2 cut(s) 5, 316
BspCNI CTCAG 1 cut(s) 805
BspPI GGATC 2 cut(s) 269, 557
BsrDI GCAATG 1 cut(s) 625
BsrI ACTGG 4 cut(s) 14, 102, 201, 808
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 5 cut(s) 261, 328, 379, 436, 562
BssSI CACGAG 1 cut(s) 731
Bst2BI CACGAG 1 cut(s) 731
Bst4CI ACNGT 4 cut(s) 388, 416, 521, 756
BstAPI GCANNNNNTGC 1 cut(s) 836
BstBAI YACGTR 1 cut(s) 718
BstC8I GCNNGC 2 cut(s) 76, 369
BstDEI CTNAG 1 cut(s) 813
BstF5I GGATG 2 cut(s) 547, 575
BstKTI GATC 5 cut(s) 264, 331, 382, 439, 565
BstMAI GTCTC 1 cut(s) 790
BstMBI GATC 5 cut(s) 261, 328, 379, 436, 562
BstMWI GCNNNNNNNGC 2 cut(s) 827, 836
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 49, 242
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 605
BstV2I GAAGAC 2 cut(s) 682, 747
BsuRI GGCC 2 cut(s) 5, 316
BtsCI GGATG 2 cut(s) 547, 575
BtsIMutI CAGTG 2 cut(s) 21, 95
Cac8I GCNNGC 2 cut(s) 76, 369
Cfr13I GGNCC 1 cut(s) 315
Csp6I GTAC 3 cut(s) 204, 427, 719
CviAII CATG 3 cut(s) 152, 773, 787
CviJI RGCY 9 cut(s) 5, 49, 67, 111, 217, 316, 596, 730, 763
CviKI_1 RGCY 9 cut(s) 5, 49, 67, 111, 217, 316, 596, 730, 763
CviQI GTAC 3 cut(s) 204, 427, 719
DdeI CTNAG 1 cut(s) 813
DpnI GATC 5 cut(s) 263, 330, 381, 438, 564
DpnII GATC 5 cut(s) 261, 328, 379, 436, 562
DraI TTTAAA 1 cut(s) 465
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 386
Ecl136II GAGCTC 1 cut(s) 730
Eco24I GRGCYC 1 cut(s) 732
Eco32I GATATC 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 730
EcoRV GATATC 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 732
FaeI CATG 3 cut(s) 155, 776, 790
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FaqI GGGAC 1 cut(s) 67
FatI CATG 3 cut(s) 151, 772, 786
FblI GTMKAC 1 cut(s) 640
Fnu4HI GCNGC 2 cut(s) 594, 822
FokI GGATG 2 cut(s) 554, 562
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 2 cut(s) 594, 822
GluI GCNGC 2 cut(s) 594, 822
GsuI CTGGAG 1 cut(s) 545
HaeIII GGCC 2 cut(s) 5, 316
HapII CCGG 2 cut(s) 50, 243
Hin1II CATG 3 cut(s) 155, 776, 790
HinfI GANTC 3 cut(s) 469, 627, 769
HpaII CCGG 2 cut(s) 50, 243
HphI GGTGA 2 cut(s) 721, 818
Hpy166II GTNNAC 3 cut(s) 95, 641, 736
Hpy188I TCNGA 1 cut(s) 632
Hpy188III TCNNGA 6 cut(s) 28, 209, 284, 338, 524, 700
Hpy8I GTNNAC 3 cut(s) 95, 641, 736
Hpy99I CGWCG 1 cut(s) 437
HpyAV CCTTC 3 cut(s) 172, 229, 582
HpyCH4III ACNGT 4 cut(s) 388, 416, 521, 756
HpyCH4IV ACGT 1 cut(s) 717
HpyCH4V TGCA 3 cut(s) 95, 230, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 827, 836
HpyF3I CTNAG 1 cut(s) 813
HpySE526I ACGT 1 cut(s) 717
Hsp92II CATG 3 cut(s) 155, 776, 790
Kzo9I GATC 5 cut(s) 261, 328, 379, 436, 562
LmnI GCTCC 1 cut(s) 615
Lsp1109I GCAGC 1 cut(s) 605
MaeII ACGT 1 cut(s) 717
MaeIII GTNAC 3 cut(s) 388, 649, 750
MalI GATC 5 cut(s) 263, 330, 381, 438, 564
MboI GATC 5 cut(s) 261, 328, 379, 436, 562
MboII GAAGA 6 cut(s) 113, 119, 157, 214, 682, 752
MhlI GDGCHC 2 cut(s) 97, 732
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 9, 123, 238, 296, 332, 374
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 221, 655
MnlI CCTC 3 cut(s) 291, 576, 794
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 464
MslI CAYNNNNRTG 1 cut(s) 804
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 2 cut(s) 50, 243
MspR9I CCNGG 2 cut(s) 51, 244
MwoI GCNNNNNNNGC 2 cut(s) 827, 836
NciI CCSGG 2 cut(s) 51, 244
NdeII GATC 5 cut(s) 261, 328, 379, 436, 562
NlaIII CATG 3 cut(s) 155, 776, 790
NmeAIII GCCGAG 1 cut(s) 50
NmuCI GTSAC 2 cut(s) 388, 649
NspI RCATGY 1 cut(s) 155
PfeI GAWTC 3 cut(s) 469, 627, 769
PkrI GCNGC 2 cut(s) 595, 823
Ppu21I YACGTR 1 cut(s) 718
Psp124BI GAGCTC 1 cut(s) 732
PspPI GGNCC 1 cut(s) 315
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 3 cut(s) 205, 428, 720
RsaNI GTAC 3 cut(s) 204, 427, 719
RseI CAYNNNNRTG 1 cut(s) 804
SacI GAGCTC 1 cut(s) 732
SaqAI TTAA 1 cut(s) 464
SatI GCNGC 2 cut(s) 594, 822
Sau3AI GATC 5 cut(s) 261, 328, 379, 436, 562
Sau96I GGNCC 1 cut(s) 315
ScaI AGTACT 1 cut(s) 205
ScrFI CCNGG 2 cut(s) 51, 244
SduI GDGCHC 2 cut(s) 97, 732
SetI ASST 9 cut(s) 164, 257, 574, 598, 612, 637, 656, 720, 732
SmiMI CAYNNNNRTG 1 cut(s) 804
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
Sse9I AATT 6 cut(s) 9, 123, 238, 296, 332, 374
SsiI CCGC 2 cut(s) 371, 822
SstI GAGCTC 1 cut(s) 732
StyD4I CCNGG 2 cut(s) 49, 242
TaaI ACNGT 4 cut(s) 388, 416, 521, 756
TaiI ACGT 1 cut(s) 720
TaqI TCGA 1 cut(s) 29
TasI AATT 6 cut(s) 9, 123, 238, 296, 332, 374
TatI WGTACW 1 cut(s) 203
TauI GCSGC 1 cut(s) 824
TfiI GAWTC 3 cut(s) 469, 627, 769
Tru1I TTAA 1 cut(s) 464
Tru9I TTAA 1 cut(s) 464
TscAI CASTG 2 cut(s) 21, 102
TseFI GTSAC 2 cut(s) 388, 649
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 2 cut(s) 388, 649
TspDTI ATGAA 3 cut(s) 461, 564, 577
TspGWI ACGGA 2 cut(s) 491, 615
TspRI CASTG 2 cut(s) 21, 102
VneI GTGCAC 1 cut(s) 93
XapI RAATTY 1 cut(s) 9
XceI RCATGY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 640
ZrmI AGTACT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.