Rw2G000580

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000805
Physical Location & Seq
Forward (+)
512646 .. 513549
904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000580.1

Sequence Viewer

Length: 447 bp
ATGGGTAGTACTCCTAACAAGGAGCCCAAGGTTCCGGTCATAGATTTTTCCGGCAACGACTTAAAGCCCGGAACAAGTTCATGGCTCGAAGCCTGCAACAAAGTCTGCAACGCACTCGAAGACTATGGCTGCTTCGTGGCAACACTGTCCGATGCAGACTGTTTAGAGCTTCACAACACCATCTTTGGCAGCTTGGACGACTTGTTTGATTTCTCCAAAGAGATCAAGCTCCAGAACACTCACCAGAAGCCTTTCAGCGGCTACTCTTCACCTAACTCTGTTCATGAAGGCTTGGGGATCGATGATCCTACAAACCCTGAGGCTACTCGGAACTTCACCAAACTTTTCTGGCCCCATGGAAATCATCATTTCTGTGAGACTGCAAATTCATATGCCAAGCTGGTGACGGGGCTAGCTAGACCAGACTGTGACAAGAATGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

148

Amino Acids

16.32

Weight (kDa)

4.94

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 11 - 95 8.6e-08 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 258
AclWI GGATC 2 cut(s) 299, 305
AcsI RAATTY 1 cut(s) 385
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 257
AluBI AGCT 5 cut(s) 169, 192, 229, 400, 416
AluI AGCT 5 cut(s) 169, 192, 229, 400, 416
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 2 cut(s) 299, 305
AoxI GGCC 1 cut(s) 350
ApeKI GCWGC 2 cut(s) 129, 189
ApoI RAATTY 1 cut(s) 385
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 2 cut(s) 76, 251
AspS9I GGNCC 1 cut(s) 351
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 4 cut(s) 233, 261, 328, 415
AsuNHI GCTAGC 1 cut(s) 412
AxyI CCTNAGG 1 cut(s) 318
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 116, 201
BccI CCATC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 413, 417
BisI GCNGC 3 cut(s) 130, 190, 259
BlsI GCNGC 3 cut(s) 131, 191, 260
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 3 cut(s) 24, 33, 353
BmrFI CCNGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 142
BmtI GCTAGC 1 cut(s) 416
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 215
BpuMI CCSGG 1 cut(s) 69
Bsa29I ATCGAT 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 27, 355
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse21I CCTNAGG 1 cut(s) 318
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 27, 355
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMII CTCAG 1 cut(s) 309
BseXI GCAGC 2 cut(s) 116, 201
BshFI GGCC 1 cut(s) 352
BshVI ATCGAT 1 cut(s) 300
BsiSI CCGG 3 cut(s) 35, 51, 69
BslI CCNNNNNNNGG 1 cut(s) 257
BsmAI GTCTC 1 cut(s) 371
BsnI GGCC 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 3 cut(s) 222, 297, 304
Bsp19I CCATGG 1 cut(s) 355
BspACI CCGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 352
BspCNI CTCAG 1 cut(s) 310
BspDI ATCGAT 1 cut(s) 300
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 3 cut(s) 24, 33, 353
BspOI GCTAGC 1 cut(s) 416
BspPI GGATC 2 cut(s) 299, 305
BssECI CCNNGG 2 cut(s) 27, 355
BssMI GATC 3 cut(s) 222, 297, 304
BssT1I CCWWGG 2 cut(s) 27, 355
Bst4CI ACNGT 3 cut(s) 147, 161, 428
Bst6I CTCTTC 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 94, 414
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 1 cut(s) 355
BstKTI GATC 3 cut(s) 225, 300, 307
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 3 cut(s) 222, 297, 304
BstSCI CCNGG 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 116, 201
BstV2I GAAGAC 1 cut(s) 126
Bsu15I ATCGAT 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 1 cut(s) 352
BsuTUI ATCGAT 1 cut(s) 300
BtgI CCRYGG 1 cut(s) 355
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 94, 414
CciI TCATGA 1 cut(s) 283
Cfr13I GGNCC 1 cut(s) 351
ClaI ATCGAT 1 cut(s) 300
Csp6I GTAC 1 cut(s) 9
CviAII CATG 3 cut(s) 81, 284, 356
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 1 cut(s) 318
DpnI GATC 3 cut(s) 224, 299, 306
DpnII GATC 3 cut(s) 222, 297, 304
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 27, 355
Eco24I GRGCYC 1 cut(s) 27
Eco81I CCTNAGG 1 cut(s) 318
EcoT14I CCWWGG 2 cut(s) 27, 355
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 2 cut(s) 27, 355
FaeI CATG 3 cut(s) 84, 287, 359
FaiI YATR 7 cut(s) 41, 82, 126, 285, 357, 391, 393
FatI CATG 3 cut(s) 80, 283, 355
FauNDI CATATG 1 cut(s) 391
Fnu4HI GCNGC 3 cut(s) 130, 190, 259
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 3 cut(s) 130, 190, 259
FspBI CTAG 2 cut(s) 413, 417
GluI GCNGC 3 cut(s) 130, 190, 259
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 1 cut(s) 352
HapII CCGG 3 cut(s) 35, 51, 69
Hin1II CATG 3 cut(s) 84, 287, 359
HpaII CCGG 3 cut(s) 35, 51, 69
HphI GGTGA 4 cut(s) 233, 261, 328, 415
Hpy188I TCNGA 2 cut(s) 151, 330
Hpy188III TCNNGA 2 cut(s) 232, 284
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 3 cut(s) 147, 161, 428
HpyCH4V TGCA 4 cut(s) 96, 108, 155, 383
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 3 cut(s) 84, 287, 359
Kzo9I GATC 3 cut(s) 222, 297, 304
LmnI GCTCC 2 cut(s) 22, 234
Lsp1109I GCAGC 2 cut(s) 116, 201
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 413, 417
MaeIII GTNAC 2 cut(s) 403, 428
MalI GATC 3 cut(s) 224, 299, 306
MboI GATC 3 cut(s) 222, 297, 304
MboII GAAGA 2 cut(s) 131, 258
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 1 cut(s) 385
MnlI CCTC 1 cut(s) 313
MroXI GAANNNNTTC 2 cut(s) 76, 251
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 258
MspI CCGG 3 cut(s) 35, 51, 69
MspR9I CCNGG 1 cut(s) 69
NciI CCSGG 1 cut(s) 69
NcoI CCATGG 1 cut(s) 355
NdeI CATATG 1 cut(s) 391
NdeII GATC 3 cut(s) 222, 297, 304
NheI GCTAGC 1 cut(s) 412
NlaIII CATG 3 cut(s) 84, 287, 359
NlaIV GGNNCC 3 cut(s) 24, 33, 353
NmuCI GTSAC 2 cut(s) 403, 428
PagI TCATGA 1 cut(s) 283
PdmI GAANNNNTTC 2 cut(s) 76, 251
PkrI GCNGC 3 cut(s) 131, 191, 260
PspN4I GGNNCC 3 cut(s) 24, 33, 353
PspPI GGNCC 1 cut(s) 351
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 3 cut(s) 130, 190, 259
Sau3AI GATC 3 cut(s) 222, 297, 304
Sau96I GGNCC 1 cut(s) 351
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 1 cut(s) 27
SetI ASST 7 cut(s) 33, 171, 194, 231, 274, 402, 418
SfaNI GCATC 1 cut(s) 142
SmiMI CAYNNNNRTG 1 cut(s) 372
Sse9I AATT 1 cut(s) 385
SsiI CCGC 1 cut(s) 258
SspMI CTAG 2 cut(s) 413, 417
StyD4I CCNGG 1 cut(s) 67
StyI CCWWGG 2 cut(s) 27, 355
TaaI ACNGT 3 cut(s) 147, 161, 428
TaqI TCGA 3 cut(s) 87, 117, 300
TasI AATT 1 cut(s) 385
TatI WGTACW 1 cut(s) 8
TauI GCSGC 1 cut(s) 261
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 2 cut(s) 403, 428
TseI GCWGC 2 cut(s) 129, 189
Tsp45I GTSAC 2 cut(s) 403, 428
TspDTI ATGAA 4 cut(s) 69, 272, 300, 378
TspRI CASTG 1 cut(s) 150
XapI RAATTY 1 cut(s) 385
XmnI GAANNNNTTC 2 cut(s) 76, 251
XspI CTAG 2 cut(s) 413, 417
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.