Rroxscaffold_2G00135880

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000805
Physical Location & Seq
Forward (+)
73383312 .. 73384671
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00135880.1

Sequence Viewer

Length: 936 bp
ATGACCCAGATTCCAGTGGTAGATTTCTCAGATCCCGAGTGCTTGAAGCCAGGGACAAGTTCATGGCTCTCGGCACGCAATCAAGTTTGCAATGCACTCGAGGATCAGTTTGGCTGTTTTACAGCGATACTGTCCAACAAAGTTTCCTTGGAACTTCACAACACCGTCTTTGGTGCCGTGAACGAGTTGTTTGAATCCCCAGACGGCATCAAAGATCAGAACAAGTACGAAAAATGGCCTTTCTGTGGCCATTTCAAGTCGAATTCTGTCCTTGAACGCTTGGGGATTTATGATCCTACAAACCCCGAAGAAATTCACAACTTTTCACATCTTTTTTGGCCTACTGGAAATGATCAATTTTGTGATGGTGTCCATTTGTACGCAAAAGTGATGAAAGAACTAGATCAAGCTGTGACGAGAATGGTATTCGAAAACCATGGTGTAGAGAAGCACCATGATGACCACATTGAATCCAGTTTTTACTGCTTTCAATTTTCAAAATATACGGAGCCTGAAAAATCTGGAAGTAATGTGGGTATAGTGAGTCATACAGATAAGAACTTCACCTCCATACTCCATCAAAACCAGATCAATGGCCTCGAGATTTATACAAAGGATGGCGAGTGGATAGGTTTCGATCCTCATCCTCTACCTTCATCCTTCATATTCATAGCAAGTGATGTATTTCAGGTTTGGAGCAATGACAGAATAAGAGCTTGTAAACACAGGGTCAACCTGATTCACTCAGATCAGATAAGATACTCGTTTGGGCTCTTCTCACTCAAACGAGGAGTAACAACCGTACCGGAGGAGCTGGTGGACAAGGATCACCCTTTAAAGTATAAGCCGTTGAATCAGGTTGAGTATATTCAAGCACAGGGTTACAAAGATAGTGAAGGACATGAGTGTAAGCTCAAGGCATTCTGTGGTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

311

Amino Acids

35.62

Weight (kDa)

5.62

Isoelectric Point (pI)

27.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 164 - 259 1.1e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AclWI GGATC 5 cut(s) 26, 111, 287, 632, 834
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 2 cut(s) 262, 312
AfaI GTAC 3 cut(s) 227, 380, 804
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 9 cut(s) 46, 194, 256, 275, 470, 491, 498, 853, 872
AjnI CCWGG 1 cut(s) 49
AloI GAACNNNNNNTCC 2 cut(s) 551, 583
AluBI AGCT 4 cut(s) 410, 716, 814, 913
AluI AGCT 4 cut(s) 410, 716, 814, 913
AlwI GGATC 5 cut(s) 26, 111, 287, 632, 834
Ama87I CYCGRG 3 cut(s) 35, 98, 599
AoxI GGCC 4 cut(s) 236, 247, 338, 595
ApoI RAATTY 2 cut(s) 262, 312
Asp700I GAANNNNTTC 1 cut(s) 312
AsuHPI GGTGA 2 cut(s) 556, 821
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 3 cut(s) 35, 98, 599
BaeI ACNNNNGTAYC 2 cut(s) 786, 819
BalI TGGCCA 1 cut(s) 249
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 774
BccI CCATC 3 cut(s) 359, 585, 611
BceAI ACGGC 3 cut(s) 161, 220, 832
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BciT130I CCWGG 1 cut(s) 51
BclI TGATCA 1 cut(s) 352
BfaI CTAG 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 51
BmeT110I CYCGRG 3 cut(s) 35, 98, 599
BmiI GGNNCC 2 cut(s) 175, 510
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 216
Bpu14I TTCGAA 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 899
BsaBI GATNNNNATC 1 cut(s) 642
BsaJI CCNNGG 3 cut(s) 50, 147, 436
BsaWI WCCGGW 1 cut(s) 805
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 3 cut(s) 14, 349, 474
Bse3DI GCAATG 2 cut(s) 97, 706
Bse8I GATNNNNATC 1 cut(s) 642
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 3 cut(s) 50, 147, 436
BseGI GGATG 3 cut(s) 622, 643, 656
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 2 cut(s) 97, 706
BseMII CTCAG 2 cut(s) 42, 759
BseNI ACTGG 3 cut(s) 14, 349, 474
BseRI GAGGAG 2 cut(s) 804, 824
BshFI GGCC 4 cut(s) 238, 249, 340, 597
BshNI GGYRCC 1 cut(s) 173
BsiHKCI CYCGRG 3 cut(s) 35, 98, 599
BsiSI CCGG 1 cut(s) 806
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 920
BsnI GGCC 4 cut(s) 238, 249, 340, 597
BsoBI CYCGRG 3 cut(s) 35, 98, 599
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 774
Bsp19I CCATGG 1 cut(s) 436
BspANI GGCC 4 cut(s) 238, 249, 340, 597
BspCNI CTCAG 2 cut(s) 41, 758
BspLI GGNNCC 2 cut(s) 175, 510
BspPI GGATC 5 cut(s) 26, 111, 287, 632, 834
BspQI GCTCTTC 1 cut(s) 779
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 97, 706
BsrI ACTGG 3 cut(s) 14, 349, 474
BssECI CCNNGG 3 cut(s) 50, 147, 436
BssT1I CCWWGG 2 cut(s) 147, 436
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 132, 166, 802
Bst6I CTCTTC 1 cut(s) 779
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 2 cut(s) 28, 745
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 3 cut(s) 622, 643, 656
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 593
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 4 cut(s) 238, 249, 340, 597
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 3 cut(s) 622, 643, 656
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 76
Csp6I GTAC 3 cut(s) 226, 379, 803
CviAII CATG 4 cut(s) 63, 437, 455, 902
CviQI GTAC 3 cut(s) 226, 379, 803
DdeI CTNAG 2 cut(s) 28, 745
DraI TTTAAA 1 cut(s) 837
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 779
EarI CTCTTC 1 cut(s) 779
Eco130I CCWWGG 2 cut(s) 147, 436
Eco24I GRGCYC 1 cut(s) 774
Eco88I CYCGRG 3 cut(s) 35, 98, 599
EcoRI GAATTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 2 cut(s) 147, 436
EcoT38I GRGCYC 1 cut(s) 774
ErhI CCWWGG 2 cut(s) 147, 436
FaeI CATG 4 cut(s) 66, 440, 458, 905
FaqI GGGAC 1 cut(s) 67
FatI CATG 4 cut(s) 62, 436, 454, 901
FbaI TGATCA 1 cut(s) 352
FokI GGATG 3 cut(s) 629, 630, 643
FriOI GRGCYC 1 cut(s) 774
FspBI CTAG 1 cut(s) 401
HaeIII GGCC 4 cut(s) 238, 249, 340, 597
HapII CCGG 1 cut(s) 806
Hin1II CATG 4 cut(s) 66, 440, 458, 905
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 6 cut(s) 10, 194, 470, 544, 739, 853
HpaII CCGG 1 cut(s) 806
HphI GGTGA 2 cut(s) 556, 821
Hpy166II GTNNAC 4 cut(s) 181, 722, 733, 820
Hpy188I TCNGA 4 cut(s) 31, 219, 748, 753
Hpy188III TCNNGA 3 cut(s) 35, 522, 601
Hpy8I GTNNAC 4 cut(s) 181, 722, 733, 820
HpyAV CCTTC 3 cut(s) 663, 670, 890
HpyCH4III ACNGT 3 cut(s) 132, 166, 802
HpyCH4V TGCA 2 cut(s) 90, 95
HpyF3I CTNAG 2 cut(s) 28, 745
Hsp92II CATG 4 cut(s) 66, 440, 458, 905
Ksp22I TGATCA 1 cut(s) 352
LguI GCTCTTC 1 cut(s) 779
LmnI GCTCC 3 cut(s) 508, 696, 811
LweI GCATC 1 cut(s) 216
MaeI CTAG 1 cut(s) 401
MaeIII GTNAC 3 cut(s) 412, 793, 881
MboII GAAGA 2 cut(s) 320, 766
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 774
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 4 cut(s) 262, 312, 356, 491
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 1 cut(s) 553
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 7 cut(s) 94, 577, 608, 651, 657, 782, 802
Mox20I TGGCCA 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 312
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 836
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 249
MspI CCGG 1 cut(s) 806
MspR9I CCNGG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 920
MvaI CCWGG 1 cut(s) 51
NcoI CCATGG 1 cut(s) 436
NlaIII CATG 4 cut(s) 66, 440, 458, 905
NlaIV GGNNCC 2 cut(s) 175, 510
NmeAIII GCCGAG 1 cut(s) 50
NmuCI GTSAC 1 cut(s) 412
NspV TTCGAA 1 cut(s) 429
PaeR7I CTCGAG 2 cut(s) 98, 599
PciSI GCTCTTC 1 cut(s) 779
PctI GAATGC 1 cut(s) 920
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 5 cut(s) 10, 194, 470, 739, 853
PleI GAGTC 1 cut(s) 552
PpsI GAGTC 1 cut(s) 552
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 175, 510
PspXI VCTCGAGB 1 cut(s) 98
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 3 cut(s) 227, 380, 804
RsaNI GTAC 3 cut(s) 226, 379, 803
RseI CAYNNNNRTG 1 cut(s) 456
SapI GCTCTTC 1 cut(s) 779
SaqAI TTAA 1 cut(s) 836
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 1 cut(s) 774
SfaNI GCATC 1 cut(s) 216
Sfr274I CTCGAG 2 cut(s) 98, 599
SfuI TTCGAA 1 cut(s) 429
SlaI CTCGAG 2 cut(s) 98, 599
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 3 cut(s) 98, 599, 914
SmoI CTYRAG 3 cut(s) 98, 599, 914
Sse9I AATT 4 cut(s) 262, 312, 356, 491
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 2 cut(s) 147, 436
TaaI ACNGT 3 cut(s) 132, 166, 802
TaqI TCGA 5 cut(s) 99, 260, 429, 600, 636
TasI AATT 4 cut(s) 262, 312, 356, 491
TfiI GAWTC 5 cut(s) 10, 194, 470, 739, 853
Tru1I TTAA 1 cut(s) 836
Tru9I TTAA 1 cut(s) 836
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 5 cut(s) 51, 407, 645, 652, 658
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 1 cut(s) 21
XapI RAATTY 2 cut(s) 262, 312
XhoI CTCGAG 2 cut(s) 98, 599
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.