MD09G1188300.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000805
Physical Location & Seq
Forward (+)
16470678 .. 16472339
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1188300.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGGTACCAGGGATGGATTTCTTCCGGTGATAGATTTCTCCAAAGAGGACTGCTTGAAGCCAGGGACAAGATCTTGGCTCTCCGTCCGCCGGGAGGTTTGCCGTGCACTGGAAGAGCTCGGTGGTTTCATGGCAATAATGCCCGACAAAGTTTTTGCTGAGCTCCACCAAACTATCTTTGGTGCACTGAAGGACTTGTTTGAGTTCCCTGCCGAACTCAAAGCCAAAAACCGATACGAAGAGAATCCATTTTGTGGCCATTTCATATATAATTCTGTGCATGAGAGCTTGGGGATTCAGAATCCCACAAACTCAGAAGAAACTCAGAAATTCACACATCTTTTCTGGCCTAACCAAAATGATCAATTCCGTGATAGTGTAGATTCATATGCAAAGGTGATGATGGAGCTTGATCGTGTTGTAACAAGAATGGTATTCGAAAACTACGGTATGGAGAAGTACCACGACGAACACATTCGATCTACTTCTCACTTTATCCAGTTTGCTAAATACAAAGAACCCAGAAAAGCTGGAAGCAATGTGGGTATAGTGAGTCACACTGACAAGAACTTCAACACAATTCTTCATCAAAATCATGTCAACGGTCTGGAGATAAACACTGACGATGACGAGTGGATAATGTTCGATCCACATCTACCTTCATCCTTCCTTTTCATAGCTGGTGATGTGTTTAAGGTATGGAGTAACGGCAGAATACGGGCTTGTAGACACAGAGTCAACGTAAGAGAAAATGACGAGGCAAGATACTCGGTTGGTTTTTTCTCTCTAAAGATAGGGGTAACAACCGTACCCAAGGAGCTTGTGGACGAAGAACACCCCTTACGTTACAAGTCACTAGACCAAGTGGAGTATATCGAAGCACAAAGGAAAAATGGAATTGAGTCCACACCGGAGGCATTCTGCGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

311

Amino Acids

35.96

Weight (kDa)

5.74

Isoelectric Point (pI)

26.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 168 - 262 4e-19 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AccB7I CCANNNNNTGG 1 cut(s) 254
AccI GTMKAC 1 cut(s) 727
AciI CCGC 2 cut(s) 88, 924
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 3 cut(s) 7, 461, 810
AfiI CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
AgsI TTSAA 2 cut(s) 58, 574
AhdI GACNNNNNGTC 1 cut(s) 734
AjnI CCWGG 2 cut(s) 8, 61
AluBI AGCT 7 cut(s) 118, 163, 288, 409, 530, 680, 820
AluI AGCT 7 cut(s) 118, 163, 288, 409, 530, 680, 820
Alw21I GWGCWC 4 cut(s) 109, 120, 165, 187
Alw44I GTGCAC 2 cut(s) 105, 183
AlwI GGATC 1 cut(s) 641
AoxI GGCC 2 cut(s) 256, 347
ApaLI GTGCAC 2 cut(s) 105, 183
ApoI RAATTY 1 cut(s) 329
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
Asp700I GAANNNNTTC 1 cut(s) 474
Asp718I GGTACC 1 cut(s) 5
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 40, 409, 695
AsuII TTCGAA 1 cut(s) 438
BaeGI GKGCMC 2 cut(s) 109, 187
BaeI ACNNNNGTAYC 2 cut(s) 792, 825
BalI TGGCCA 1 cut(s) 258
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 2 cut(s) 120, 165
Bbv12I GWGCWC 4 cut(s) 109, 120, 165, 187
BccI CCATC 2 cut(s) 8, 397
BceAI ACGGC 2 cut(s) 87, 724
BcgI CGANNNNNNTGC 2 cut(s) 750, 784
BciT130I CCWGG 2 cut(s) 10, 63
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 92
BfaI CTAG 1 cut(s) 857
BglII AGATCT 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 159
Bme1390I CCNGG 3 cut(s) 10, 63, 92
BmeRI GACNNNNNGTC 1 cut(s) 734
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 3 cut(s) 10, 63, 92
BpmI CTGGAG 1 cut(s) 629
Bpu1102I GCTNAGC 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 651
BsaJI CCNNGG 3 cut(s) 9, 62, 813
BsaWI WCCGGW 2 cut(s) 25, 910
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
Bse1I ACTGG 2 cut(s) 114, 499
Bse3DI GCAATG 1 cut(s) 544
Bse8I GATNNNNATC 1 cut(s) 651
BseBI CCWGG 2 cut(s) 10, 63
BseDI CCNNGG 3 cut(s) 9, 62, 813
BseGI GGATG 2 cut(s) 19, 662
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 3 cut(s) 150, 327, 338
BseNI ACTGG 2 cut(s) 114, 499
BseSI GKGCMC 2 cut(s) 109, 187
BshFI GGCC 2 cut(s) 258, 349
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 4 cut(s) 109, 120, 165, 187
BsiSI CCGG 3 cut(s) 26, 91, 911
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 2 cut(s) 258, 349
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 4 cut(s) 109, 120, 165, 187
Bsp143I GATC 5 cut(s) 71, 361, 412, 479, 646
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 2 cut(s) 88, 924
BspANI GGCC 2 cut(s) 258, 349
BspCNI CTCAG 3 cut(s) 151, 326, 337
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 1 cut(s) 641
BspQI GCTCTTC 1 cut(s) 108
BspT104I TTCGAA 1 cut(s) 438
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 544
BsrI ACTGG 2 cut(s) 114, 499
BssECI CCNNGG 3 cut(s) 9, 62, 813
BssMI GATC 5 cut(s) 71, 361, 412, 479, 646
BssT1I CCWWGG 1 cut(s) 813
Bst2UI CCWGG 2 cut(s) 10, 63
Bst4CI ACNGT 3 cut(s) 449, 605, 808
Bst6I CTCTTC 2 cut(s) 108, 234
BstBI TTCGAA 1 cut(s) 438
BstDEI CTNAG 3 cut(s) 159, 313, 324
BstF5I GGATG 2 cut(s) 19, 662
BstKTI GATC 5 cut(s) 74, 364, 415, 482, 649
BstMBI GATC 5 cut(s) 71, 361, 412, 479, 646
BstNI CCWGG 2 cut(s) 10, 63
BstSCI CCNGG 3 cut(s) 8, 61, 90
BstSLI GKGCMC 2 cut(s) 109, 187
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BsuRI GGCC 2 cut(s) 258, 349
BtsCI GGATG 2 cut(s) 19, 662
BtsIMutI CAGTG 4 cut(s) 107, 185, 558, 618
Csp6I GTAC 3 cut(s) 6, 460, 809
CviAII CATG 3 cut(s) 130, 281, 596
CviQI GTAC 3 cut(s) 6, 460, 809
DdeI CTNAG 3 cut(s) 159, 313, 324
DpnI GATC 5 cut(s) 73, 363, 414, 481, 648
DpnII GATC 5 cut(s) 71, 361, 412, 479, 646
DriI GACNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 108, 234
Eam1105I GACNNNNNGTC 1 cut(s) 734
EarI CTCTTC 2 cut(s) 108, 234
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 2 cut(s) 118, 163
Eco130I CCWWGG 1 cut(s) 813
Eco24I GRGCYC 2 cut(s) 120, 165
Eco53kI GAGCTC 2 cut(s) 118, 163
Eco57I CTGAAG 1 cut(s) 209
EcoICRI GAGCTC 2 cut(s) 118, 163
EcoRII CCWGG 2 cut(s) 8, 61
EcoT14I CCWWGG 1 cut(s) 813
EcoT38I GRGCYC 2 cut(s) 120, 165
ErhI CCWWGG 1 cut(s) 813
FaeI CATG 3 cut(s) 133, 284, 599
FaqI GGGAC 1 cut(s) 79
FatI CATG 3 cut(s) 129, 280, 595
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 1 cut(s) 727
FokI GGATG 2 cut(s) 26, 649
FriOI GRGCYC 2 cut(s) 120, 165
FspBI CTAG 1 cut(s) 857
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 2 cut(s) 258, 349
HapII CCGG 3 cut(s) 26, 91, 911
Hin1II CATG 3 cut(s) 133, 284, 599
HincII GTYRAC 2 cut(s) 601, 739
HindII GTYRAC 2 cut(s) 601, 739
HinfI GANTC 7 cut(s) 244, 295, 301, 383, 553, 735, 902
HpaII CCGG 3 cut(s) 26, 91, 911
HphI GGTGA 3 cut(s) 40, 409, 695
Hpy166II GTNNAC 7 cut(s) 107, 185, 601, 728, 739, 826, 906
Hpy188I TCNGA 3 cut(s) 300, 316, 327
Hpy188III TCNNGA 1 cut(s) 608
Hpy8I GTNNAC 7 cut(s) 107, 185, 601, 728, 739, 826, 906
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 3 cut(s) 184, 669, 676
HpyCH4III ACNGT 3 cut(s) 449, 605, 808
HpyCH4IV ACGT 2 cut(s) 741, 844
HpyCH4V TGCA 4 cut(s) 107, 185, 280, 392
HpyF3I CTNAG 3 cut(s) 159, 313, 324
HpySE526I ACGT 2 cut(s) 741, 844
Hsp92II CATG 3 cut(s) 133, 284, 599
KpnI GGTACC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 5 cut(s) 71, 361, 412, 479, 646
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 3 cut(s) 168, 406, 817
MaeI CTAG 1 cut(s) 857
MaeII ACGT 2 cut(s) 741, 844
MaeIII GTNAC 6 cut(s) 421, 554, 704, 799, 845, 852
MalI GATC 5 cut(s) 73, 363, 414, 481, 648
MboI GATC 5 cut(s) 71, 361, 412, 479, 646
MboII GAAGA 6 cut(s) 14, 125, 251, 329, 575, 842
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 4 cut(s) 109, 120, 165, 187
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 5 cut(s) 271, 329, 365, 579, 897
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 3 cut(s) 562, 744, 911
MnlI CCTC 4 cut(s) 40, 88, 751, 907
Mox20I TGGCCA 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 258
MseI TTAA 1 cut(s) 693
Msp20I TGGCCA 1 cut(s) 258
MspI CCGG 3 cut(s) 26, 91, 911
MspR9I CCNGG 3 cut(s) 10, 63, 92
Mva1269I GAATGC 1 cut(s) 917
MvaI CCWGG 2 cut(s) 10, 63
NciI CCSGG 1 cut(s) 92
NdeI CATATG 1 cut(s) 388
NdeII GATC 5 cut(s) 71, 361, 412, 479, 646
NlaIII CATG 3 cut(s) 133, 284, 599
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 2 cut(s) 554, 852
NspV TTCGAA 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 108
PctI GAATGC 1 cut(s) 917
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 4 cut(s) 244, 295, 301, 383
PflMI CCANNNNNTGG 1 cut(s) 254
PleI GAGTC 3 cut(s) 561, 743, 910
PpsI GAGTC 3 cut(s) 561, 743, 910
Psp124BI GAGCTC 2 cut(s) 120, 165
Psp6I CCWGG 2 cut(s) 8, 61
PspGI CCWGG 2 cut(s) 8, 61
PspN4I GGNNCC 1 cut(s) 7
PsrI GAACNNNNNNTAC 2 cut(s) 825, 857
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 3 cut(s) 7, 461, 810
RsaNI GTAC 3 cut(s) 6, 460, 809
SacI GAGCTC 2 cut(s) 120, 165
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 1 cut(s) 693
Sau3AI GATC 5 cut(s) 71, 361, 412, 479, 646
SchI GAGTC 3 cut(s) 562, 744, 911
ScrFI CCNGG 3 cut(s) 10, 63, 92
SduI GDGCHC 4 cut(s) 109, 120, 165, 187
SfuI TTCGAA 1 cut(s) 438
Sse9I AATT 5 cut(s) 271, 329, 365, 579, 897
SsiI CCGC 2 cut(s) 88, 924
SspMI CTAG 1 cut(s) 857
SstI GAGCTC 2 cut(s) 120, 165
StyD4I CCNGG 3 cut(s) 8, 61, 90
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 3 cut(s) 449, 605, 808
TaiI ACGT 2 cut(s) 744, 847
TaqI TCGA 4 cut(s) 438, 478, 645, 876
TasI AATT 5 cut(s) 271, 329, 365, 579, 897
TfiI GAWTC 4 cut(s) 244, 295, 301, 383
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TscAI CASTG 4 cut(s) 114, 192, 565, 625
TseFI GTSAC 2 cut(s) 554, 852
Tsp45I GTSAC 2 cut(s) 554, 852
TspDTI ATGAA 6 cut(s) 118, 253, 375, 575, 651, 664
TspGWI ACGGA 2 cut(s) 73, 359
TspRI CASTG 4 cut(s) 114, 192, 565, 625
Van91I CCANNNNNTGG 1 cut(s) 254
VneI GTGCAC 2 cut(s) 105, 183
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 820
XmiI GTMKAC 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.