pycom09g10520

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000805
Physical Location & Seq
Forward (+)
8604959 .. 8606104
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10520.3

Sequence Viewer

Length: 933 bp
ATGGGTACCAGGGATGGATTTCTTCCGGTGATAGATTTCTCCAAAGAGGACTGCTTGAAGCCAGGGACAAGTTCTTGGCTCTCCGTCCGCCGGGAGGTTTGCCGTGCACTGGAAGAGCTCGGTGGTTTCATGGCAATAATGCCCGACAAAGTTTCTGCTGAGCTCCACCAAACCATCTTTGGTGCACTGAAGGACTTGTTTGAGTTCCCTGCCGAACTCAAAGCCGAAAACCGATACGAAGAGAATCCATTTTGTGGCCATTTCATATATAATTCTGTTCATGAGAGCTTGGGGATTCAGAATCCCACACACTCAGAAGAAACTCAGAAATTCACAAATCTTTTCTGGCCTAACCAAAATGATCGATTCAGTGATAGCGTAGATTCATATGCAAAGGTGATGATGGAGCTTGATCATGTTGTAACAAGAATGGTATTCGAAAACTACGGTGTGGAGAAGTACCACGATGAACACATTCGATCTACTTCTCACTTTATCCAGTTTGCTAAATACAAGGAACCCACAAAAGCTGGCAGCGATGTGGGTTTAGAAAGTCACACTGACAAGAACTTCAACACAATTCTTCATCAAAATCATGTCAATGGTCTGGAGATAAACACGGACCATGACGAGTGGATAATGATCGATCCACATCTACCTTCATCCGTCCTGTTCATAGCTGGTGATATGTTTAAGGTATGGAGTAACGGCAGAATACGGGCTTGTAGACACAGAGTCAACGTAAGAGAAAATGATGAGGCAAGATACTCGGTTGGATTTTTCTCTCTAAAGATAGGAGTAACAACCGTACCCAAGGAGCTTGTGGACGAAGAACACCCCTTACGTTACAAGTCACTAGACCAAGTGGAGTATATCGAAGTACAAAGGAAAAATGGAATTGAGTGCACACCGGAGGCATTCTGCGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

311

Amino Acids

35.73

Weight (kDa)

5.38

Isoelectric Point (pI)

28.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AccB7I CCANNNNNTGG 1 cut(s) 254
AccI GTMKAC 1 cut(s) 727
AciI CCGC 2 cut(s) 88, 924
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 4 cut(s) 7, 461, 810, 882
AfiI CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
AgsI TTSAA 2 cut(s) 58, 574
AhdI GACNNNNNGTC 1 cut(s) 734
AjnI CCWGG 2 cut(s) 8, 61
AluBI AGCT 7 cut(s) 118, 163, 288, 409, 530, 680, 820
AluI AGCT 7 cut(s) 118, 163, 288, 409, 530, 680, 820
Alw21I GWGCWC 5 cut(s) 109, 120, 165, 187, 908
Alw44I GTGCAC 3 cut(s) 105, 183, 904
AlwI GGATC 1 cut(s) 641
AoxI GGCC 2 cut(s) 256, 347
ApaLI GTGCAC 3 cut(s) 105, 183, 904
ApeKI GCWGC 1 cut(s) 534
ApoI RAATTY 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 474
Asp718I GGTACC 1 cut(s) 5
AspS9I GGNCC 1 cut(s) 622
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 40, 409, 695
AsuII TTCGAA 1 cut(s) 438
AvaII GGWCC 1 cut(s) 622
BaeGI GKGCMC 3 cut(s) 109, 187, 908
BaeI ACNNNNGTAYC 2 cut(s) 792, 825
BalI TGGCCA 1 cut(s) 258
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 2 cut(s) 120, 165
Bbv12I GWGCWC 5 cut(s) 109, 120, 165, 187, 908
BbvI GCAGC 1 cut(s) 546
BccI CCATC 3 cut(s) 8, 182, 397
BceAI ACGGC 2 cut(s) 87, 724
BcgI CGANNNNNNTGC 2 cut(s) 750, 784
BciT130I CCWGG 2 cut(s) 10, 63
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 92
BfaI CTAG 1 cut(s) 857
BisI GCNGC 1 cut(s) 535
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 536
Bme1390I CCNGG 3 cut(s) 10, 63, 92
Bme18I GGWCC 1 cut(s) 622
BmeRI GACNNNNNGTC 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 2 cut(s) 7, 519
BmrFI CCNGG 3 cut(s) 10, 63, 92
BpmI CTGGAG 1 cut(s) 629
Bpu1102I GCTNAGC 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 92
Bsa29I ATCGAT 2 cut(s) 364, 645
BsaBI GATNNNNATC 2 cut(s) 641, 651
BsaJI CCNNGG 3 cut(s) 9, 62, 813
BsaWI WCCGGW 2 cut(s) 25, 910
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
Bse1I ACTGG 2 cut(s) 114, 499
Bse8I GATNNNNATC 2 cut(s) 641, 651
BseBI CCWGG 2 cut(s) 10, 63
BseCI ATCGAT 2 cut(s) 364, 645
BseDI CCNNGG 3 cut(s) 9, 62, 813
BseGI GGATG 2 cut(s) 19, 662
BseJI GATNNNNATC 2 cut(s) 641, 651
BseLI CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
BseMII CTCAG 3 cut(s) 150, 327, 338
BseNI ACTGG 2 cut(s) 114, 499
BseSI GKGCMC 3 cut(s) 109, 187, 908
BseXI GCAGC 1 cut(s) 546
BshFI GGCC 2 cut(s) 258, 349
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 2 cut(s) 364, 645
BsiHKAI GWGCWC 5 cut(s) 109, 120, 165, 187, 908
BsiSI CCGG 3 cut(s) 26, 91, 911
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 4 cut(s) 90, 94, 109, 254
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 2 cut(s) 258, 349
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 5 cut(s) 109, 120, 165, 187, 908
Bsp143I GATC 5 cut(s) 361, 412, 479, 642, 646
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 2 cut(s) 88, 924
BspANI GGCC 2 cut(s) 258, 349
BspCNI CTCAG 3 cut(s) 151, 326, 337
BspDI ATCGAT 2 cut(s) 364, 645
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 7, 519
BspPI GGATC 1 cut(s) 641
BspQI GCTCTTC 1 cut(s) 108
BspT104I TTCGAA 1 cut(s) 438
BspT107I GGYRCC 1 cut(s) 5
BsrI ACTGG 2 cut(s) 114, 499
BssECI CCNNGG 3 cut(s) 9, 62, 813
BssMI GATC 5 cut(s) 361, 412, 479, 642, 646
BssT1I CCWWGG 1 cut(s) 813
Bst2UI CCWGG 2 cut(s) 10, 63
Bst4CI ACNGT 2 cut(s) 449, 808
Bst6I CTCTTC 2 cut(s) 108, 234
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 532
BstDEI CTNAG 3 cut(s) 159, 313, 324
BstF5I GGATG 2 cut(s) 19, 662
BstKTI GATC 5 cut(s) 364, 415, 482, 645, 649
BstMBI GATC 5 cut(s) 361, 412, 479, 642, 646
BstNI CCWGG 2 cut(s) 10, 63
BstSCI CCNGG 3 cut(s) 8, 61, 90
BstSLI GKGCMC 3 cut(s) 109, 187, 908
BstV1I GCAGC 1 cut(s) 546
Bsu15I ATCGAT 2 cut(s) 364, 645
BsuRI GGCC 2 cut(s) 258, 349
BsuTUI ATCGAT 2 cut(s) 364, 645
BtgZI GCGATG 1 cut(s) 552
BtsCI GGATG 2 cut(s) 19, 662
BtsIMutI CAGTG 4 cut(s) 107, 185, 376, 558
Cac8I GCNNGC 1 cut(s) 532
CciI TCATGA 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 622
ClaI ATCGAT 2 cut(s) 364, 645
Csp6I GTAC 4 cut(s) 6, 460, 809, 881
CviAII CATG 5 cut(s) 130, 281, 416, 596, 626
CviQI GTAC 4 cut(s) 6, 460, 809, 881
DdeI CTNAG 3 cut(s) 159, 313, 324
DpnI GATC 5 cut(s) 363, 414, 481, 644, 648
DpnII GATC 5 cut(s) 361, 412, 479, 642, 646
DriI GACNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 108, 234
Eam1105I GACNNNNNGTC 1 cut(s) 734
EarI CTCTTC 2 cut(s) 108, 234
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 2 cut(s) 118, 163
Eco130I CCWWGG 1 cut(s) 813
Eco24I GRGCYC 2 cut(s) 120, 165
Eco47I GGWCC 1 cut(s) 622
Eco53kI GAGCTC 2 cut(s) 118, 163
Eco57I CTGAAG 1 cut(s) 209
EcoICRI GAGCTC 2 cut(s) 118, 163
EcoRII CCWGG 2 cut(s) 8, 61
EcoT14I CCWWGG 1 cut(s) 813
EcoT38I GRGCYC 2 cut(s) 120, 165
ErhI CCWWGG 1 cut(s) 813
FaeI CATG 5 cut(s) 133, 284, 419, 599, 629
FaqI GGGAC 1 cut(s) 79
FatI CATG 5 cut(s) 129, 280, 415, 595, 625
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 727
Fnu4HI GCNGC 1 cut(s) 535
FokI GGATG 2 cut(s) 26, 649
FriOI GRGCYC 2 cut(s) 120, 165
Fsp4HI GCNGC 1 cut(s) 535
FspBI CTAG 1 cut(s) 857
GluI GCNGC 1 cut(s) 535
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 2 cut(s) 258, 349
HapII CCGG 3 cut(s) 26, 91, 911
Hin1II CATG 5 cut(s) 133, 284, 419, 599, 629
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 6 cut(s) 244, 295, 301, 366, 383, 735
HpaII CCGG 3 cut(s) 26, 91, 911
HphI GGTGA 3 cut(s) 40, 409, 695
Hpy166II GTNNAC 6 cut(s) 107, 185, 728, 739, 826, 906
Hpy188I TCNGA 3 cut(s) 300, 316, 327
Hpy188III TCNNGA 2 cut(s) 281, 608
Hpy8I GTNNAC 6 cut(s) 107, 185, 728, 739, 826, 906
HpyAV CCTTC 2 cut(s) 184, 669
HpyCH4III ACNGT 2 cut(s) 449, 808
HpyCH4IV ACGT 2 cut(s) 741, 844
HpyCH4V TGCA 4 cut(s) 107, 185, 392, 906
HpyF3I CTNAG 3 cut(s) 159, 313, 324
HpySE526I ACGT 2 cut(s) 741, 844
Hsp92II CATG 5 cut(s) 133, 284, 419, 599, 629
KpnI GGTACC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 5 cut(s) 361, 412, 479, 642, 646
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 3 cut(s) 168, 406, 817
Lsp1109I GCAGC 1 cut(s) 546
MaeI CTAG 1 cut(s) 857
MaeII ACGT 2 cut(s) 741, 844
MaeIII GTNAC 6 cut(s) 421, 554, 704, 799, 845, 852
MalI GATC 5 cut(s) 363, 414, 481, 644, 648
MboI GATC 5 cut(s) 361, 412, 479, 642, 646
MboII GAAGA 6 cut(s) 14, 125, 251, 329, 575, 842
MhlI GDGCHC 5 cut(s) 109, 120, 165, 187, 908
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 4 cut(s) 271, 329, 579, 897
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 1 cut(s) 744
MmeI TCCRAC 1 cut(s) 754
MnlI CCTC 4 cut(s) 40, 88, 751, 907
Mox20I TGGCCA 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 258
MseI TTAA 1 cut(s) 693
MslI CAYNNNNRTG 1 cut(s) 600
Msp20I TGGCCA 1 cut(s) 258
MspI CCGG 3 cut(s) 26, 91, 911
MspR9I CCNGG 3 cut(s) 10, 63, 92
Mva1269I GAATGC 1 cut(s) 917
MvaI CCWGG 2 cut(s) 10, 63
NciI CCSGG 1 cut(s) 92
NdeI CATATG 1 cut(s) 388
NdeII GATC 5 cut(s) 361, 412, 479, 642, 646
NlaIII CATG 5 cut(s) 133, 284, 419, 599, 629
NlaIV GGNNCC 2 cut(s) 7, 519
NmuCI GTSAC 2 cut(s) 554, 852
NspV TTCGAA 1 cut(s) 438
PagI TCATGA 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 108
PctI GAATGC 1 cut(s) 917
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 5 cut(s) 244, 295, 301, 366, 383
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 1 cut(s) 536
PleI GAGTC 1 cut(s) 743
PpsI GAGTC 1 cut(s) 743
Psp124BI GAGCTC 2 cut(s) 120, 165
Psp6I CCWGG 2 cut(s) 8, 61
PspGI CCWGG 2 cut(s) 8, 61
PspN4I GGNNCC 2 cut(s) 7, 519
PspPI GGNCC 1 cut(s) 622
PsrI GAACNNNNNNTAC 2 cut(s) 825, 857
RsaI GTAC 4 cut(s) 7, 461, 810, 882
RsaNI GTAC 4 cut(s) 6, 460, 809, 881
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 2 cut(s) 120, 165
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 1 cut(s) 535
Sau3AI GATC 5 cut(s) 361, 412, 479, 642, 646
Sau96I GGNCC 1 cut(s) 622
SchI GAGTC 1 cut(s) 744
ScrFI CCNGG 3 cut(s) 10, 63, 92
SduI GDGCHC 5 cut(s) 109, 120, 165, 187, 908
SfuI TTCGAA 1 cut(s) 438
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 600
Sse9I AATT 4 cut(s) 271, 329, 579, 897
SsiI CCGC 2 cut(s) 88, 924
SspMI CTAG 1 cut(s) 857
SstI GAGCTC 2 cut(s) 120, 165
StyD4I CCNGG 3 cut(s) 8, 61, 90
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 2 cut(s) 449, 808
TaiI ACGT 2 cut(s) 744, 847
TaqI TCGA 5 cut(s) 364, 438, 478, 645, 876
TasI AATT 4 cut(s) 271, 329, 579, 897
TatI WGTACW 1 cut(s) 880
TfiI GAWTC 5 cut(s) 244, 295, 301, 366, 383
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TscAI CASTG 4 cut(s) 114, 192, 376, 565
TseFI GTSAC 2 cut(s) 554, 852
TseI GCWGC 1 cut(s) 534
Tsp45I GTSAC 2 cut(s) 554, 852
TspDTI ATGAA 8 cut(s) 118, 253, 269, 375, 483, 575, 651, 664
TspGWI ACGGA 3 cut(s) 73, 635, 655
TspRI CASTG 4 cut(s) 114, 192, 376, 565
Van91I CCANNNNNTGG 1 cut(s) 254
VneI GTGCAC 3 cut(s) 105, 183, 904
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 820
XmiI GTMKAC 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.