MD11G1275200.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000805
Physical Location & Seq
Reverse (-)
39211538 .. 39213337
1800 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1275200.v1.1.491

Sequence Viewer

Length: 927 bp
ATGACCACGAGCACCCAAGCAGCAGCGGCCAAAATTCCAGTGGTAGATTTCTCCCTGGACGGCATGAAGCCCGGGACAAGTTCTTGGCTCTCAATACGCGACGACGTTTGCCATGCACTTGAAGAGCTTGGCTGTTTCATAGCAATCCTGCCCAACAAACTTCCTTTGGAGGAGCTCCACAACTCCTTCGTTGGTGCATTGAAAGAGTTGTTAGATTTCCCCATGGACGTCAAAGTGCGAGACAAAAAAGGGGCCTATTTATCTATCCATCCTCCCCATGAAGGCTTGGGGGTGCTTAATAACACCTCTGAAGAAATTCAGAAGTTCACTCGTCATTTTTGGCCTAATGGAAACGATCTTTTTTGTGAGGGTGCACTTGAGTATGCAAAGGCGATGGCAGAACTAGATCAAGTTGTGACAAGAATGGTATTTGAAAACTACGGTGTAGAGAAGTATCATGATGCTCACGTTCAATCAACTTCTTACCATCTCAGATTCAATAAATACAAAAATCCCAAGAAAACTGGAATGGACGTGGGGTTAAAACCTCATACGGACAATAGCTTCTCCTCCATACTCCATCAAAATCAGGTCTGTGGTGTGGAGATATGTACAAAGGATGGTGACTGGATAGTTTTTTATCCTCCGCCGTCATCCGTTATATACCTAGCCGGAGATGTATTTCAGATTTGGAGCAACGACAGAATACGACCTTGTAGACATAGAGTTTCGTTGACGGAAGATGAGGTAAGATTCTCGATTGGGCAATTCACATTTATGAAAGAGGTGGTGAGCATACCAAACGAACTCGTGGACGAGGATCACCCTCGACTGTACAAGCCACTGGATCATTTAGAATTTTTCAAAGAAGTAAAACTCATTGACGGTAAGCCTGATCGTTTAGTCCAGGAATATTGTCGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

309

Amino Acids

35.19

Weight (kDa)

5.71

Isoelectric Point (pI)

25.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 163 - 257 7.4e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 718
AccII CGCG 1 cut(s) 99
AciI CCGC 2 cut(s) 26, 647
AclWI GGATC 2 cut(s) 828, 855
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 3 cut(s) 33, 315, 857
AcuI CTGAAG 1 cut(s) 330
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 2 cut(s) 613, 836
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 6 cut(s) 122, 202, 434, 473, 499, 865
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 2 cut(s) 54, 906
AluBI AGCT 3 cut(s) 127, 175, 564
AluI AGCT 3 cut(s) 127, 175, 564
Alw21I GWGCWC 3 cut(s) 14, 177, 376
Alw26I GTCTC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 2 cut(s) 828, 855
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 3 cut(s) 27, 252, 341
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 20, 23
ApoI RAATTY 3 cut(s) 33, 315, 857
Asp700I GAANNNNTTC 1 cut(s) 315
AspS9I GGNCC 1 cut(s) 252
AsuC2I CCSGG 2 cut(s) 72, 73
AsuHPI GGTGA 3 cut(s) 635, 802, 815
AvaI CYCGRG 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 177
BauI CACGAG 2 cut(s) 7, 809
Bbv12I GWGCWC 3 cut(s) 14, 177, 376
BbvI GCAGC 2 cut(s) 32, 35
BccI CCATC 5 cut(s) 276, 388, 495, 588, 614
BceAI ACGGC 2 cut(s) 76, 634
BciT130I CCWGG 2 cut(s) 56, 908
BcnI CCSGG 2 cut(s) 72, 73
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 2 cut(s) 404, 668
BisI GCNGC 3 cut(s) 21, 24, 27
BlsI GCNGC 3 cut(s) 22, 25, 28
Bme1390I CCNGG 4 cut(s) 56, 72, 73, 908
BmeT110I CYCGRG 1 cut(s) 71
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 4 cut(s) 56, 72, 73, 908
BmsI GCATC 1 cut(s) 451
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 2 cut(s) 72, 73
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 3 cut(s) 54, 71, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse1I ACTGG 4 cut(s) 38, 529, 632, 849
BseBI CCWGG 2 cut(s) 56, 908
BseDI CCNNGG 3 cut(s) 54, 71, 222
BseGI GGATG 3 cut(s) 268, 625, 653
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMII CTCAG 1 cut(s) 505
BseNI ACTGG 4 cut(s) 38, 529, 632, 849
BseRI GAGGAG 2 cut(s) 185, 559
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 2 cut(s) 32, 35
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 29, 254, 343
BsiHKAI GWGCWC 3 cut(s) 14, 177, 376
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 2 cut(s) 72, 672
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 3 cut(s) 29, 254, 343
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 3 cut(s) 14, 177, 376
Bsp1407I TGTACA 2 cut(s) 611, 834
Bsp143I GATC 5 cut(s) 355, 406, 820, 847, 895
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 2 cut(s) 26, 647
BspANI GGCC 3 cut(s) 29, 254, 343
BspCNI CTCAG 1 cut(s) 504
BspFNI CGCG 1 cut(s) 99
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 253
BspPI GGATC 2 cut(s) 828, 855
BspQI GCTCTTC 1 cut(s) 117
BsrGI TGTACA 2 cut(s) 611, 834
BsrI ACTGG 4 cut(s) 38, 529, 632, 849
BssECI CCNNGG 3 cut(s) 54, 71, 222
BssMI GATC 5 cut(s) 355, 406, 820, 847, 895
BssNI GRCGYC 1 cut(s) 228
BssSI CACGAG 2 cut(s) 7, 809
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 2 cut(s) 7, 809
Bst2UI CCWGG 2 cut(s) 56, 908
Bst4CI ACNGT 3 cut(s) 443, 834, 887
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 228
BstAUI TGTACA 2 cut(s) 611, 834
BstDEI CTNAG 1 cut(s) 491
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 268, 625, 653
BstFNI CGCG 1 cut(s) 99
BstKTI GATC 5 cut(s) 358, 409, 823, 850, 898
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 5 cut(s) 355, 406, 820, 847, 895
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 2 cut(s) 56, 908
BstSCI CCNGG 4 cut(s) 54, 70, 71, 906
BstSLI GKGCMC 1 cut(s) 376
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 32, 35
BsuRI GGCC 3 cut(s) 29, 254, 343
BtgI CCRYGG 1 cut(s) 222
BtgZI GCGATG 1 cut(s) 407
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 3 cut(s) 268, 625, 653
BtsIMutI CAGTG 2 cut(s) 45, 842
CciI TCATGA 1 cut(s) 457
Cfr13I GGNCC 1 cut(s) 252
Cfr9I CCCGGG 1 cut(s) 71
Csp6I GTAC 2 cut(s) 612, 835
CviAII CATG 5 cut(s) 64, 113, 223, 278, 458
CviQI GTAC 2 cut(s) 612, 835
DdeI CTNAG 1 cut(s) 491
DpnI GATC 5 cut(s) 357, 408, 822, 849, 897
DpnII GATC 5 cut(s) 355, 406, 820, 847, 895
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EciI GGCGGA 1 cut(s) 636
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 252
EcoRII CCWGG 2 cut(s) 54, 906
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 5 cut(s) 67, 116, 226, 281, 461
FaqI GGGAC 1 cut(s) 88
FatI CATG 5 cut(s) 63, 112, 222, 277, 457
FblI GTMKAC 1 cut(s) 718
Fnu4HI GCNGC 3 cut(s) 21, 24, 27
FokI GGATG 3 cut(s) 255, 632, 640
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 3 cut(s) 21, 24, 27
FspBI CTAG 2 cut(s) 404, 668
GluI GCNGC 3 cut(s) 21, 24, 27
HaeIII GGCC 3 cut(s) 29, 254, 343
HapII CCGG 2 cut(s) 72, 672
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 5 cut(s) 67, 116, 226, 281, 461
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 2 cut(s) 495, 753
HpaII CCGG 2 cut(s) 72, 672
HphI GGTGA 3 cut(s) 635, 802, 815
Hpy166II GTNNAC 5 cut(s) 327, 374, 719, 735, 814
Hpy188I TCNGA 4 cut(s) 310, 321, 494, 687
Hpy188III TCNNGA 2 cut(s) 458, 757
Hpy8I GTNNAC 5 cut(s) 327, 374, 719, 735, 814
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 2 cut(s) 196, 275
HpyCH4III ACNGT 3 cut(s) 443, 834, 887
HpyCH4IV ACGT 4 cut(s) 105, 228, 468, 534
HpyCH4V TGCA 4 cut(s) 116, 197, 374, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 1 cut(s) 491
HpySE526I ACGT 4 cut(s) 105, 228, 468, 534
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 5 cut(s) 67, 116, 226, 281, 461
Kzo9I GATC 5 cut(s) 355, 406, 820, 847, 895
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 3 cut(s) 172, 180, 693
Lsp1109I GCAGC 2 cut(s) 32, 35
LweI GCATC 1 cut(s) 451
MaeI CTAG 2 cut(s) 404, 668
MaeII ACGT 4 cut(s) 105, 228, 468, 534
MaeIII GTNAC 2 cut(s) 415, 623
MalI GATC 5 cut(s) 357, 408, 822, 849, 897
MboI GATC 5 cut(s) 355, 406, 820, 847, 895
MboII GAAGA 3 cut(s) 134, 323, 752
MhlI GDGCHC 3 cut(s) 14, 177, 376
MluCI AATT 4 cut(s) 33, 315, 767, 857
MroXI GAANNNNTTC 1 cut(s) 315
MseI TTAA 2 cut(s) 297, 542
MslI CAYNNNNRTG 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 72, 672
MspR9I CCNGG 4 cut(s) 56, 72, 73, 908
MvaI CCWGG 2 cut(s) 56, 908
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 26
NciI CCSGG 2 cut(s) 72, 73
NcoI CCATGG 1 cut(s) 222
NdeII GATC 5 cut(s) 355, 406, 820, 847, 895
NlaIII CATG 5 cut(s) 67, 116, 226, 281, 461
NlaIV GGNNCC 1 cut(s) 253
NmuCI GTSAC 2 cut(s) 415, 623
PagI TCATGA 1 cut(s) 457
PciSI GCTCTTC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 2 cut(s) 495, 753
PfoI TCCNGGA 1 cut(s) 906
PkrI GCNGC 3 cut(s) 22, 25, 28
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 54, 906
PspGI CCWGG 2 cut(s) 54, 906
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 1 cut(s) 252
RsaI GTAC 2 cut(s) 613, 836
RsaNI GTAC 2 cut(s) 612, 835
RseI CAYNNNNRTG 1 cut(s) 776
SacI GAGCTC 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 2 cut(s) 297, 542
SatI GCNGC 3 cut(s) 21, 24, 27
Sau3AI GATC 5 cut(s) 355, 406, 820, 847, 895
Sau96I GGNCC 1 cut(s) 252
ScrFI CCNGG 4 cut(s) 56, 72, 73, 908
SduI GDGCHC 3 cut(s) 14, 177, 376
SfaNI GCATC 1 cut(s) 451
SmaI CCCGGG 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 4 cut(s) 33, 315, 767, 857
SsiI CCGC 2 cut(s) 26, 647
SspI AATATT 1 cut(s) 914
SspMI CTAG 2 cut(s) 404, 668
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 4 cut(s) 54, 70, 71, 906
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 3 cut(s) 443, 834, 887
TaiI ACGT 4 cut(s) 108, 231, 471, 537
TaqI TCGA 2 cut(s) 758, 829
TasI AATT 4 cut(s) 33, 315, 767, 857
TatI WGTACW 2 cut(s) 611, 834
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 2 cut(s) 495, 753
Tru1I TTAA 2 cut(s) 297, 542
Tru9I TTAA 2 cut(s) 297, 542
TscAI CASTG 2 cut(s) 45, 849
TseFI GTSAC 2 cut(s) 415, 623
TseI GCWGC 2 cut(s) 20, 23
Tsp45I GTSAC 2 cut(s) 415, 623
TspDTI ATGAA 4 cut(s) 80, 127, 294, 794
TspGWI ACGGA 3 cut(s) 569, 646, 752
TspMI CCCGGG 1 cut(s) 71
TspRI CASTG 2 cut(s) 45, 849
VneI GTGCAC 1 cut(s) 372
XapI RAATTY 3 cut(s) 33, 315, 857
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 2 cut(s) 404, 668
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.