MD11G1275100.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000805
Physical Location & Seq
Forward (+)
39192608 .. 39193526
919 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1275100.v1.1.491

Sequence Viewer

Length: 432 bp
ATGAGGCCTTGGGGGTGCTTAGTGACACTTCTGGAGAAATTCAGAAGTTCACTCGGTGCACTTGAGTATGCAAAAGCGATGGAAGAACTAAATCAAGTTGTGACAAGAATAGTATTTGAAAACTACGGTGTAGAGAAGTATCATGATGCTCACGTTCAATCAACTTCTTACCATCTCCGATTCAATAAATACAAAAATCCCAAGAAAACTGGGGATGTGGGGTTAACCCCTCATACGGACAATAGCTTCTCCACCATACTTCATCAAAACCAGGTCTGTGGTCTGGAGATGTATACAAAGGATGGTGACTGGATAGTCTTTGATCCACTGCCGTCATCCGTTATATACCTAGAAGGAGATGTATTTCAGATTTGGAGCAATGATAAAATACGACCTTGTAGACATAAAGTTTCGTTGACGACGATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

144

Amino Acids

16.47

Weight (kDa)

7.0

Isoelectric Point (pI)

28.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 58 - 138 4.1e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AccI GTMKAC 2 cut(s) 293, 400
AclWI GGATC 1 cut(s) 317
AcsI RAATTY 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 235
AgsI TTSAA 3 cut(s) 119, 158, 184
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 1 cut(s) 246
AluI AGCT 1 cut(s) 246
Alw21I GWGCWC 1 cut(s) 61
Alw44I GTGCAC 1 cut(s) 57
AlwI GGATC 1 cut(s) 317
AoxI GGCC 1 cut(s) 5
ApaLI GTGCAC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 317
BaeGI GKGCMC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 61
BccI CCATC 4 cut(s) 73, 180, 296, 418
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 272
BfaI CTAG 1 cut(s) 350
Bme1390I CCNGG 1 cut(s) 272
BmrFI CCNGG 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 219
BmsI GCATC 1 cut(s) 136
BmuI ACTGGG 1 cut(s) 219
BpmI CTGGAG 2 cut(s) 53, 305
BpuEI CTTGAG 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 8
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 214, 314
Bse3DI GCAATG 1 cut(s) 385
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 1 cut(s) 8
BseGI GGATG 3 cut(s) 220, 307, 335
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMI GCAATG 1 cut(s) 385
BseNI ACTGG 2 cut(s) 214, 314
BseSI GKGCMC 1 cut(s) 61
BshFI GGCC 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 235
BsnI GGCC 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 1 cut(s) 322
BspANI GGCC 1 cut(s) 7
BspHI TCATGA 1 cut(s) 142
BspPI GGATC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 2 cut(s) 214, 314
BssECI CCNNGG 1 cut(s) 8
BssMI GATC 1 cut(s) 322
BssNAI GTATAC 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 8
Bst1107I GTATAC 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 128
BstDEI CTNAG 1 cut(s) 19
BstF5I GGATG 3 cut(s) 220, 307, 335
BstKTI GATC 1 cut(s) 325
BstMBI GATC 1 cut(s) 322
BstNI CCWGG 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 278
BstZ17I GTATAC 1 cut(s) 294
BsuRI GGCC 1 cut(s) 7
BtgZI GCGATG 1 cut(s) 92
BtsCI GGATG 3 cut(s) 220, 307, 335
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 142
CsiI ACCWGGT 1 cut(s) 270
CviAII CATG 1 cut(s) 143
CviJI RGCY 2 cut(s) 7, 246
CviKI_1 RGCY 2 cut(s) 7, 246
DdeI CTNAG 1 cut(s) 19
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
DraIII CACNNNGTG 1 cut(s) 56
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 8
Eco147I AGGCCT 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 8
FaeI CATG 1 cut(s) 146
FaiI YATR 8 cut(s) 69, 144, 234, 257, 294, 344, 346, 405
FatI CATG 1 cut(s) 142
FblI GTMKAC 2 cut(s) 293, 400
FokI GGATG 3 cut(s) 227, 314, 322
FspBI CTAG 1 cut(s) 350
GsuI CTGGAG 2 cut(s) 53, 305
HaeIII GGCC 1 cut(s) 7
Hin1II CATG 1 cut(s) 146
HincII GTYRAC 2 cut(s) 225, 417
HindII GTYRAC 2 cut(s) 225, 417
HinfI GANTC 1 cut(s) 180
HpaI GTTAAC 1 cut(s) 225
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 6 cut(s) 50, 59, 225, 294, 401, 417
Hpy188I TCNGA 3 cut(s) 44, 179, 369
Hpy188III TCNNGA 3 cut(s) 32, 143, 284
Hpy8I GTNNAC 6 cut(s) 50, 59, 225, 294, 401, 417
Hpy99I CGWCG 1 cut(s) 424
HpyAV CCTTC 1 cut(s) 347
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 59, 71
HpyF3I CTNAG 1 cut(s) 19
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 1 cut(s) 146
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 1 cut(s) 322
LmnI GCTCC 1 cut(s) 375
LpnPI CCDG 6 cut(s) 17, 195, 257, 269, 284, 295
LweI GCATC 1 cut(s) 136
MabI ACCWGGT 1 cut(s) 270
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 22, 100, 305
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 1 cut(s) 38
MnlI CCTC 1 cut(s) 240
MseI TTAA 1 cut(s) 224
MspR9I CCNGG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 272
NdeII GATC 1 cut(s) 322
NlaIII CATG 1 cut(s) 146
NmuCI GTSAC 3 cut(s) 22, 100, 305
PagI TCATGA 1 cut(s) 142
PceI AGGCCT 1 cut(s) 7
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PfeI GAWTC 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
SaqAI TTAA 1 cut(s) 224
Sau3AI GATC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 61
SetI ASST 5 cut(s) 156, 248, 276, 351, 397
SexAI ACCWGGT 1 cut(s) 270
SfaNI GCATC 1 cut(s) 136
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 1 cut(s) 38
SseBI AGGCCT 1 cut(s) 7
SspMI CTAG 1 cut(s) 350
StuI AGGCCT 1 cut(s) 7
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 8
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 1 cut(s) 156
TasI AATT 1 cut(s) 38
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 3 cut(s) 22, 100, 305
Tsp45I GTSAC 3 cut(s) 22, 100, 305
TspDTI ATGAA 1 cut(s) 251
TspGWI ACGGA 2 cut(s) 251, 328
TspRI CASTG 1 cut(s) 333
VneI GTGCAC 1 cut(s) 57
XapI RAATTY 1 cut(s) 38
XmiI GTMKAC 2 cut(s) 293, 400
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.