MD03G1207600.v1.1

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000805
Physical Location & Seq
Reverse (-)
28392939 .. 28394372
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1207600.v1.1.491

Sequence Viewer

Length: 843 bp
ATGACCACTATCATTGCAACCCAAACAGTTCCCAAAATTCCAGTTGTCGATTTCTCCAGGGAGGACTGCTTCAAGGCAGGGTCAAGTTCTTGGCTCTCAGTACGAGCGGAAGTTTGCCGTGCTCTTGAAGAGTTTAGCTGTTTCGTGATGACAGACCTAAATCAAGTTGTGACTAGAATGGTATTCGAAAACTACACTGCAGAGAAATATCATGGTGATCACATTCAATCGACTATTTATTCTCTCCGATTCACAAAATATAAGGAACTCAAGGAAGTAGAAATGGGAATGGAAAAATTCGCTCACGCAGATGCAACCTTTTCCACCATGCTTCATCAAAATCATGTAAAAGGTCTTGAGATACTTACAAAAGATGACAAATGGATTGAGTTTGATCCTCAGCTGCCTTCATCCTTCATATTCATGGCATGTGATGGATTTCAGCATGTAGGTTGTATAGATTTTAAAACATATATAGGCTTAGGACAGGCGGCTATATATATAACATATATAAGCGGCTATACCATACTAATGATAATACCCACTCATGTATTAATAAATCTTAGGGATGACGCTTGCATATGGTTTGTTTGGCCTGGGTTTGGAGCAATGACAGAATACGATCTTGTAAACAGAGTCATTATAAAGCAAAGTAGTGTAAGATTCTCGGTTGGGCTATTTTGCTATATTGAAGGGCTGATAACCATTCCGAACGAGGTTATCGATAAGGATCACCCCATACAGTATAAGCCATTCCATCAGTTGGATTATTTGAAATATTTTCTGGAACAGTATCGTATTAGTGGAGTTCTGGGTTCTATCAAGGAGTATTGCGGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

281

Amino Acids

32.24

Weight (kDa)

5.58

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 87 - 151 1.7e-06 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AccB7I CCANNNNNTGG 1 cut(s) 763
AccBSI CCGCTC 1 cut(s) 107
AciI CCGC 4 cut(s) 107, 491, 516, 834
AclWI GGATC 2 cut(s) 389, 738
AcsI RAATTY 2 cut(s) 36, 296
AfaI GTAC 1 cut(s) 102
AfiI CCNNNNNNNGG 2 cut(s) 602, 763
AgsI TTSAA 5 cut(s) 73, 128, 227, 692, 775
AjnI CCWGG 2 cut(s) 56, 595
AluBI AGCT 2 cut(s) 138, 403
AluI AGCT 2 cut(s) 138, 403
Alw21I GWGCWC 1 cut(s) 124
AlwI GGATC 2 cut(s) 389, 738
AoxI GGCC 1 cut(s) 593
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 2 cut(s) 36, 296
AseI ATTAAT 1 cut(s) 554
AsuHPI GGTGA 2 cut(s) 227, 725
AsuII TTCGAA 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 124
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 390
BccI CCATC 2 cut(s) 428, 765
BceAI ACGGC 1 cut(s) 102
BciT130I CCWGG 2 cut(s) 58, 597
BclI TGATCA 1 cut(s) 217
BfaI CTAG 1 cut(s) 174
BfmI CTRYAG 1 cut(s) 198
BisI GCNGC 3 cut(s) 404, 492, 517
BlsI GCNGC 3 cut(s) 405, 493, 518
Bme1390I CCNGG 2 cut(s) 58, 597
BmrFI CCNGG 2 cut(s) 58, 597
BmsI GCATC 1 cut(s) 301
BpmI CTGGAG 1 cut(s) 40
Bpu10I CCTNAGC 2 cut(s) 399, 481
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 2 cut(s) 254, 377
Bsa29I ATCGAT 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 729
BsaJI CCNNGG 2 cut(s) 57, 596
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 763
Bse1I ACTGG 1 cut(s) 41
Bse3DI GCAATG 2 cut(s) 12, 615
Bse8I GATNNNNATC 1 cut(s) 729
BseBI CCWGG 2 cut(s) 58, 597
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 2 cut(s) 57, 596
BseGI GGATG 2 cut(s) 410, 574
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 2 cut(s) 602, 763
BseMI GCAATG 2 cut(s) 12, 615
BseMII CTCAG 2 cut(s) 111, 413
BseNI ACTGG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 390
BshFI GGCC 1 cut(s) 595
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 124
BslI CCNNNNNNNGG 2 cut(s) 602, 763
BsnI GGCC 1 cut(s) 595
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 4 cut(s) 217, 394, 622, 730
BspACI CCGC 4 cut(s) 107, 491, 516, 834
BspANI GGCC 1 cut(s) 595
BspCNI CTCAG 2 cut(s) 110, 412
BspDI ATCGAT 1 cut(s) 723
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 2 cut(s) 389, 738
BspT104I TTCGAA 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 2 cut(s) 12, 615
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 57, 596
BssMI GATC 4 cut(s) 217, 394, 622, 730
Bst2UI CCWGG 2 cut(s) 58, 597
Bst4CI ACNGT 3 cut(s) 28, 744, 792
Bst6I CTCTTC 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 4 cut(s) 97, 399, 481, 563
BstF5I GGATG 2 cut(s) 410, 574
BstKTI GATC 4 cut(s) 220, 397, 625, 733
BstMBI GATC 4 cut(s) 217, 394, 622, 730
BstNI CCWGG 2 cut(s) 58, 597
BstNSI RCATGY 2 cut(s) 432, 449
BstSCI CCNGG 2 cut(s) 56, 595
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 390
Bsu15I ATCGAT 1 cut(s) 723
BsuRI GGCC 1 cut(s) 595
BsuTUI ATCGAT 1 cut(s) 723
BtsCI GGATG 2 cut(s) 410, 574
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 577
ClaI ATCGAT 1 cut(s) 723
CseI GACGC 1 cut(s) 581
Csp6I GTAC 1 cut(s) 101
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 7 cut(s) 212, 328, 344, 424, 429, 446, 548
CviQI GTAC 1 cut(s) 101
DdeI CTNAG 4 cut(s) 97, 399, 481, 563
DpnI GATC 4 cut(s) 219, 396, 624, 732
DpnII GATC 4 cut(s) 217, 394, 622, 730
DraI TTTAAA 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 56, 595
FaeI CATG 7 cut(s) 215, 331, 347, 427, 432, 449, 551
FatI CATG 7 cut(s) 211, 327, 343, 423, 428, 445, 547
FauI CCCGC 1 cut(s) 827
FauNDI CATATG 1 cut(s) 581
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 3 cut(s) 404, 492, 517
FokI GGATG 2 cut(s) 397, 581
Fsp4HI GCNGC 3 cut(s) 404, 492, 517
FspBI CTAG 1 cut(s) 174
GluI GCNGC 3 cut(s) 404, 492, 517
GsuI CTGGAG 1 cut(s) 40
HaeIII GGCC 1 cut(s) 595
HgaI GACGC 1 cut(s) 581
Hin1II CATG 7 cut(s) 215, 331, 347, 427, 432, 449, 551
HinfI GANTC 3 cut(s) 249, 636, 663
HphI GGTGA 2 cut(s) 227, 725
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 2 cut(s) 248, 711
Hpy188III TCNNGA 4 cut(s) 125, 145, 356, 785
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 3 cut(s) 417, 424, 686
HpyCH4III ACNGT 3 cut(s) 28, 744, 792
HpyCH4V TGCA 4 cut(s) 17, 200, 314, 579
HpyF3I CTNAG 4 cut(s) 97, 399, 481, 563
Hsp92II CATG 7 cut(s) 215, 331, 347, 427, 432, 449, 551
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 4 cut(s) 217, 394, 622, 730
LmnI GCTCC 1 cut(s) 605
LpnPI CCDG 9 cut(s) 43, 54, 63, 70, 473, 582, 609, 770, 797
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 169
MalI GATC 4 cut(s) 219, 396, 624, 732
MbiI CCGCTC 1 cut(s) 107
MboI GATC 4 cut(s) 217, 394, 622, 730
MboII GAAGA 1 cut(s) 140
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 2 cut(s) 36, 296
MlyI GAGTC 1 cut(s) 645
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 3 cut(s) 55, 408, 709
MseI TTAA 2 cut(s) 465, 554
MslI CAYNNNNRTG 3 cut(s) 309, 422, 530
MspA1I CMGCKG 1 cut(s) 403
MspR9I CCNGG 2 cut(s) 58, 597
MvaI CCWGG 2 cut(s) 58, 597
NdeI CATATG 1 cut(s) 581
NdeII GATC 4 cut(s) 217, 394, 622, 730
NlaIII CATG 7 cut(s) 215, 331, 347, 427, 432, 449, 551
NmuCI GTSAC 1 cut(s) 169
NspI RCATGY 2 cut(s) 432, 449
NspV TTCGAA 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 720
PfeI GAWTC 2 cut(s) 249, 663
PflMI CCANNNNNTGG 1 cut(s) 763
PkrI GCNGC 3 cut(s) 405, 493, 518
PleI GAGTC 1 cut(s) 644
PpsI GAGTC 1 cut(s) 644
PshBI ATTAAT 1 cut(s) 554
PsiI TTATAA 1 cut(s) 644
Psp6I CCWGG 2 cut(s) 56, 595
PspGI CCWGG 2 cut(s) 56, 595
PstI CTGCAG 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 1 cut(s) 102
RsaNI GTAC 1 cut(s) 101
RseI CAYNNNNRTG 3 cut(s) 309, 422, 530
SaqAI TTAA 2 cut(s) 465, 554
SatI GCNGC 3 cut(s) 404, 492, 517
Sau3AI GATC 4 cut(s) 217, 394, 622, 730
SchI GAGTC 1 cut(s) 645
ScrFI CCNGG 2 cut(s) 58, 597
SduI GDGCHC 1 cut(s) 124
SetI ASST 7 cut(s) 140, 159, 320, 355, 405, 454, 720
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 186
SmiMI CAYNNNNRTG 3 cut(s) 309, 422, 530
SmlI CTYRAG 2 cut(s) 269, 356
SmoI CTYRAG 2 cut(s) 269, 356
Sse9I AATT 2 cut(s) 36, 296
SsiI CCGC 4 cut(s) 107, 491, 516, 834
SspI AATATT 1 cut(s) 779
SspMI CTAG 1 cut(s) 174
StyD4I CCNGG 2 cut(s) 56, 595
TaaI ACNGT 3 cut(s) 28, 744, 792
TaqI TCGA 4 cut(s) 48, 186, 230, 723
TasI AATT 2 cut(s) 36, 296
TauI GCSGC 2 cut(s) 494, 519
TfiI GAWTC 2 cut(s) 249, 663
Tru1I TTAA 2 cut(s) 465, 554
Tru9I TTAA 2 cut(s) 465, 554
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 4 cut(s) 323, 399, 406, 412
TspRI CASTG 1 cut(s) 202
Van91I CCANNNNNTGG 1 cut(s) 763
VspI ATTAAT 1 cut(s) 554
XapI RAATTY 2 cut(s) 36, 296
XceI RCATGY 2 cut(s) 432, 449
XspI CTAG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.