pycom08g20210

oxidoreductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000805
Physical Location & Seq
Forward (+)
20084076 .. 20084776
701 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20210.2

Sequence Viewer

Length: 516 bp
ATGGCTTCCAGTACCCAAACAGCGGCCAAAATTCCAGTAGTAGATTTCTCCCAAGAGGACTGCTGGAAGTCAGGGACAAGTGCTTGGCTCTCAGTACGCAGAGACATTTGTAATGCTTTCGAAGAGTTTGGTTGTTTCCTGGCAGTCCTTCCCAACAAAGTTTCCCGGGAGCTTAATGACACCATGTTTGGGACATTCAACGAGTTGTTTGATGTCCCCGACCAAATTAAAGCGCAATTTCCTCAGGAAAAACCTCTGGTTGAGGGTTACGTAAGGCTGCCTACCCGTGAATACATGTCATTTCATTCAAGTACTGAAAAAATAAAGAACGTCACACGTCAACTTTGGCCGGAAGGAAATGACCAGTTTCGTGAGAGTGCTGATCGGTATTCAAAAGTGATGACAGAATTAAATCAAGTTGTGACGAAAATGGTATTTGAAAACTACAAGGTAGAGAAATATCACGATCGATCACATTCAATCGACCATGCCCATTACCGTACTTGTAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

172

Amino Acids

19.99

Weight (kDa)

6.52

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 23
AcoI YGGCCR 2 cut(s) 24, 347
AcsI RAATTY 1 cut(s) 30
AfaI GTAC 4 cut(s) 13, 96, 313, 502
AfiI CCNNNNNNNGG 2 cut(s) 22, 189
AflIII ACRYGT 2 cut(s) 294, 335
AgsI TTSAA 5 cut(s) 199, 309, 393, 440, 480
AjiI CACGTC 1 cut(s) 338
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
Alw26I GTCTC 1 cut(s) 96
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 2 cut(s) 24, 347
ApeKI GCWGC 1 cut(s) 277
ApoI RAATTY 1 cut(s) 30
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspLEI GCGC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 166, 167
AsuII TTCGAA 1 cut(s) 120
AvaI CYCGRG 1 cut(s) 165
AxyI CCTNAGG 1 cut(s) 243
BbvI GCAGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 140
BcnI CCSGG 2 cut(s) 166, 167
BcoDI GTCTC 1 cut(s) 96
BisI GCNGC 2 cut(s) 24, 278
BlsI GCNGC 2 cut(s) 25, 279
BmcAI AGTACT 1 cut(s) 313
Bme1390I CCNGG 3 cut(s) 140, 166, 167
BmeT110I CYCGRG 1 cut(s) 165
BmgBI CACGTC 1 cut(s) 338
BmrFI CCNGG 3 cut(s) 140, 166, 167
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 2 cut(s) 166, 167
Bsa29I ATCGAT 1 cut(s) 469
BsaAI YACGTR 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 189
Bse1I ACTGG 3 cut(s) 9, 35, 364
Bse21I CCTNAGG 1 cut(s) 243
BseBI CCWGG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 22, 189
BseMII CTCAG 2 cut(s) 105, 257
BseNI ACTGG 3 cut(s) 9, 35, 364
BseXI GCAGC 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 469
BshFI GGCC 2 cut(s) 26, 349
BshVI ATCGAT 1 cut(s) 469
BsiEI CGRYCG 1 cut(s) 469
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 2 cut(s) 166, 350
BslFI GGGAC 3 cut(s) 88, 200, 205
BslI CCNNNNNNNGG 2 cut(s) 22, 189
BsmAI GTCTC 1 cut(s) 96
BsmFI GGGAC 3 cut(s) 88, 200, 205
BsnI GGCC 2 cut(s) 26, 349
BsoBI CYCGRG 1 cut(s) 165
Bsp119I TTCGAA 1 cut(s) 120
Bsp143I GATC 3 cut(s) 382, 466, 470
BspACI CCGC 1 cut(s) 23
BspANI GGCC 2 cut(s) 26, 349
BspCNI CTCAG 2 cut(s) 104, 256
BspDI ATCGAT 1 cut(s) 469
BspT104I TTCGAA 1 cut(s) 120
BsrI ACTGG 3 cut(s) 9, 35, 364
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 382, 466, 470
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 500
Bst6I CTCTTC 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 91, 243
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 3 cut(s) 385, 469, 473
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 3 cut(s) 382, 466, 470
BstMCI CGRYCG 1 cut(s) 469
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 3 cut(s) 138, 164, 165
BstSNI TACGTA 1 cut(s) 271
BstV1I GCAGC 1 cut(s) 264
Bsu15I ATCGAT 1 cut(s) 469
Bsu36I CCTNAGG 1 cut(s) 243
BsuRI GGCC 2 cut(s) 26, 349
BsuTUI ATCGAT 1 cut(s) 469
BtrI CACGTC 1 cut(s) 338
CfoI GCGC 1 cut(s) 235
Cfr9I CCCGGG 1 cut(s) 165
ClaI ATCGAT 1 cut(s) 469
Csp6I GTAC 4 cut(s) 12, 95, 312, 501
CviAII CATG 3 cut(s) 184, 295, 488
CviJI RGCY 6 cut(s) 5, 26, 88, 172, 277, 349
CviKI_1 RGCY 6 cut(s) 5, 26, 88, 172, 277, 349
CviQI GTAC 4 cut(s) 12, 95, 312, 501
DdeI CTNAG 2 cut(s) 91, 243
DpnI GATC 3 cut(s) 384, 468, 472
DpnII GATC 3 cut(s) 382, 466, 470
EaeI YGGCCR 2 cut(s) 24, 347
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco105I TACGTA 1 cut(s) 271
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 138
FaeI CATG 3 cut(s) 187, 298, 491
FaiI YATR 3 cut(s) 185, 296, 489
FaqI GGGAC 3 cut(s) 88, 200, 205
FatI CATG 3 cut(s) 183, 294, 487
Fnu4HI GCNGC 2 cut(s) 24, 278
Fsp4HI GCNGC 2 cut(s) 24, 278
GlaI GCGC 1 cut(s) 234
GluI GCNGC 2 cut(s) 24, 278
HaeIII GGCC 2 cut(s) 26, 349
HapII CCGG 2 cut(s) 166, 350
HhaI GCGC 1 cut(s) 235
Hin1II CATG 3 cut(s) 187, 298, 491
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HincII GTYRAC 1 cut(s) 341
HindII GTYRAC 1 cut(s) 341
HpaII CCGG 2 cut(s) 166, 350
Hpy166II GTNNAC 1 cut(s) 341
Hpy188III TCNNGA 3 cut(s) 245, 371, 464
Hpy8I GTNNAC 1 cut(s) 341
HpyAV CCTTC 2 cut(s) 158, 347
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 3 cut(s) 270, 330, 337
HpyF3I CTNAG 2 cut(s) 91, 243
HpySE526I ACGT 3 cut(s) 270, 330, 337
Hsp92II CATG 3 cut(s) 187, 298, 491
HspAI GCGC 1 cut(s) 233
Kzo9I GATC 3 cut(s) 382, 466, 470
LmnI GCTCC 1 cut(s) 169
Lsp1109I GCAGC 1 cut(s) 264
MaeII ACGT 3 cut(s) 270, 330, 337
MaeIII GTNAC 3 cut(s) 266, 331, 421
MalI GATC 3 cut(s) 384, 468, 472
MboI GATC 3 cut(s) 382, 466, 470
MboII GAAGA 1 cut(s) 134
MluCI AATT 4 cut(s) 30, 225, 236, 407
MnlI CCTC 4 cut(s) 49, 252, 256, 264
MseI TTAA 3 cut(s) 174, 228, 410
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 166, 350
MspR9I CCNGG 3 cut(s) 140, 166, 167
MvaI CCWGG 1 cut(s) 140
NciI CCSGG 2 cut(s) 166, 167
NdeII GATC 3 cut(s) 382, 466, 470
NlaIII CATG 3 cut(s) 187, 298, 491
NmuCI GTSAC 2 cut(s) 331, 421
NspI RCATGY 1 cut(s) 298
NspV TTCGAA 1 cut(s) 120
PciI ACATGT 1 cut(s) 294
PkrI GCNGC 2 cut(s) 25, 279
Ple19I CGATCG 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 271
PscI ACATGT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PvuI CGATCG 1 cut(s) 469
RsaI GTAC 4 cut(s) 13, 96, 313, 502
RsaNI GTAC 4 cut(s) 12, 95, 312, 501
SaqAI TTAA 3 cut(s) 174, 228, 410
SatI GCNGC 2 cut(s) 24, 278
Sau3AI GATC 3 cut(s) 382, 466, 470
ScaI AGTACT 1 cut(s) 313
ScrFI CCNGG 3 cut(s) 140, 166, 167
SetI ASST 6 cut(s) 174, 256, 273, 333, 340, 453
SfuI TTCGAA 1 cut(s) 120
SmaI CCCGGG 1 cut(s) 167
SnaBI TACGTA 1 cut(s) 271
Sse9I AATT 4 cut(s) 30, 225, 236, 407
SsiI CCGC 1 cut(s) 23
StyD4I CCNGG 3 cut(s) 138, 164, 165
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 3 cut(s) 273, 333, 340
TaqI TCGA 3 cut(s) 120, 469, 483
TasI AATT 4 cut(s) 30, 225, 236, 407
TatI WGTACW 1 cut(s) 311
TauI GCSGC 1 cut(s) 26
Tru1I TTAA 3 cut(s) 174, 228, 410
Tru9I TTAA 3 cut(s) 174, 228, 410
TseFI GTSAC 2 cut(s) 331, 421
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 2 cut(s) 331, 421
TspDTI ATGAA 1 cut(s) 293
TspMI CCCGGG 1 cut(s) 165
XapI RAATTY 1 cut(s) 30
XceI RCATGY 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 165
ZrmI AGTACT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.