FvH4_3g22161

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000979
Physical Location & Seq
Reverse (-)
15204834 .. 15205456
623 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22161.t1

Sequence Viewer

Length: 543 bp
ATGAAGAGACAAGTTAGGGCATACCATGCTGAAGCCAAATGGTTCCACAACAACGACACACCAACAATGGACGAATACATGGAAGTAGCCCTTGTCACTTCAGCCTACTCAATGCTAGCAACCACATCCTTTGTTGGAATGGGAGATATTGTTACTAAAGACTCTTTCGAGTGGATCTTCAGTGACCCTAAGATGGTAAAGGCTTCAGAAGTAGTTTGCAGACTCATGGATGACATTGTATCCCACAAGTTTGAGCAAAAGAGAGGACATGTTGCCTCGGCCGTGGAATGTTACATGACACAATACGGTGCGACAGAAGAAGAAACCATAATCGAATTTCGAAAACAAGTGAGTGATGCATGGAAGGACATAAATGAAGAGTGCCTCCACCCTACCTCTCTCGACATGCCTCTCTTGATGCGAATTGTGAATTTAACACGTGTGATAGATGTAGTGTACAAGTTTGAAGATGGTTACACTCATGCTGAAACCATACTCAAAGATTTTGTAGCATCTCTACTGGTAGATCCTGTGACTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

181

Amino Acids

20.67

Weight (kDa)

4.92

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth_C PF03936 1 - 123 2.8e-48 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 1 - 123 2e-39 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 182, 521
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 2 cut(s) 335, 430
AcuI CTGAAG 4 cut(s) 51, 84, 163, 189
AcvI CACGTG 1 cut(s) 440
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 1 cut(s) 193
AflIII ACRYGT 3 cut(s) 268, 437, 439
AgsI TTSAA 1 cut(s) 467
AlwI GGATC 2 cut(s) 182, 521
AoxI GGCC 1 cut(s) 279
ApoI RAATTY 2 cut(s) 335, 430
AsuII TTCGAA 1 cut(s) 340
AsuNHI GCTAGC 1 cut(s) 115
BbrPI CACGTG 1 cut(s) 440
BccI CCATC 2 cut(s) 187, 464
BceAI ACGGC 1 cut(s) 266
BciVI GTATCC 1 cut(s) 250
BfaI CTAG 1 cut(s) 116
BfuI GTATCC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 44
BmsI GCATC 3 cut(s) 346, 408, 521
BmtI GCTAGC 1 cut(s) 119
Bpu14I TTCGAA 1 cut(s) 340
BsaAI YACGTR 1 cut(s) 440
BsaJI CCNNGG 2 cut(s) 276, 282
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 525
BseDI CCNNGG 2 cut(s) 276, 282
BseGI GGATG 2 cut(s) 125, 235
BseLI CCNNNNNNNGG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 525
BseX3I CGGCCG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 193
BsnI GGCC 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 340
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 2 cut(s) 174, 526
BspANI GGCC 1 cut(s) 281
BspLI GGNNCC 1 cut(s) 44
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 2 cut(s) 182, 521
BspT104I TTCGAA 1 cut(s) 340
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 1 cut(s) 525
BssECI CCNNGG 2 cut(s) 276, 282
BssMI GATC 2 cut(s) 174, 526
Bst4CI ACNGT 2 cut(s) 308, 538
Bst6I CTCTTC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 456
BstBAI YACGTR 1 cut(s) 440
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 189
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 2 cut(s) 125, 235
BstKTI GATC 2 cut(s) 177, 529
BstMBI GATC 2 cut(s) 174, 526
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNSI RCATGY 2 cut(s) 272, 409
BstX2I RGATCY 2 cut(s) 174, 526
BstYI RGATCY 2 cut(s) 174, 526
BstZI CGGCCG 1 cut(s) 279
BsuI GTATCC 1 cut(s) 250
BsuRI GGCC 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 282
BtsCI GGATG 2 cut(s) 125, 235
BtsIMutI CAGTG 1 cut(s) 187
Cac8I GCNNGC 1 cut(s) 117
Csp6I GTAC 1 cut(s) 457
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 8 cut(s) 26, 79, 226, 269, 295, 360, 406, 482
CviJI RGCY 5 cut(s) 35, 89, 104, 203, 281
CviKI_1 RGCY 5 cut(s) 35, 89, 104, 203, 281
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 1 cut(s) 189
DpnI GATC 2 cut(s) 176, 528
DpnII GATC 2 cut(s) 174, 526
EaeI YGGCCR 1 cut(s) 279
EagI CGGCCG 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 279
Eco52I CGGCCG 1 cut(s) 279
Eco57I CTGAAG 4 cut(s) 51, 84, 163, 189
Eco72I CACGTG 1 cut(s) 440
EcoT22I ATGCAT 1 cut(s) 361
FaeI CATG 8 cut(s) 29, 82, 229, 272, 298, 363, 409, 485
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FatI CATG 8 cut(s) 25, 78, 225, 268, 294, 359, 405, 481
FokI GGATG 2 cut(s) 112, 242
FspBI CTAG 1 cut(s) 116
HaeIII GGCC 1 cut(s) 281
Hin1II CATG 8 cut(s) 29, 82, 229, 272, 298, 363, 409, 485
HinfI GANTC 2 cut(s) 161, 222
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 2 cut(s) 401, 415
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 2 cut(s) 308, 538
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 2 cut(s) 219, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 8 cut(s) 29, 82, 229, 272, 298, 363, 409, 485
Kzo9I GATC 2 cut(s) 174, 526
LpnPI CCDG 1 cut(s) 506
LweI GCATC 3 cut(s) 346, 408, 521
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 6 cut(s) 94, 151, 182, 290, 473, 532
MalI GATC 2 cut(s) 176, 528
MboI GATC 2 cut(s) 174, 526
MboII GAAGA 6 cut(s) 16, 169, 329, 332, 389, 479
MflI RGATCY 2 cut(s) 174, 526
MluCI AATT 3 cut(s) 335, 423, 430
MlyI GAGTC 2 cut(s) 155, 216
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 5 cut(s) 257, 286, 395, 406, 420
Mph1103I ATGCAT 1 cut(s) 361
MseI TTAA 1 cut(s) 434
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 2 cut(s) 174, 526
NheI GCTAGC 1 cut(s) 115
NlaIII CATG 8 cut(s) 29, 82, 229, 272, 298, 363, 409, 485
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 257
NmuCI GTSAC 3 cut(s) 94, 182, 532
NsiI ATGCAT 1 cut(s) 361
NspI RCATGY 2 cut(s) 272, 409
NspV TTCGAA 1 cut(s) 340
PciI ACATGT 1 cut(s) 268
PleI GAGTC 2 cut(s) 155, 216
PmaCI CACGTG 1 cut(s) 440
PmlI CACGTG 1 cut(s) 440
PpsI GAGTC 2 cut(s) 155, 216
Ppu21I YACGTR 1 cut(s) 440
PscI ACATGT 1 cut(s) 268
PspCI CACGTG 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 44
PsuI RGATCY 2 cut(s) 174, 526
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SaqAI TTAA 1 cut(s) 434
Sau3AI GATC 2 cut(s) 174, 526
SchI GAGTC 2 cut(s) 155, 216
SetI ASST 2 cut(s) 398, 442
SfaNI GCATC 3 cut(s) 346, 408, 521
SfuI TTCGAA 1 cut(s) 340
Sse9I AATT 3 cut(s) 335, 423, 430
SspMI CTAG 1 cut(s) 116
TaaI ACNGT 2 cut(s) 308, 538
TaiI ACGT 1 cut(s) 442
TaqI TCGA 4 cut(s) 168, 333, 340, 402
TasI AATT 3 cut(s) 335, 423, 430
TatI WGTACW 1 cut(s) 456
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 3 cut(s) 94, 182, 532
Tsp45I GTSAC 3 cut(s) 94, 182, 532
TspDTI ATGAA 2 cut(s) 17, 390
TspRI CASTG 1 cut(s) 187
XapI RAATTY 2 cut(s) 335, 430
XceI RCATGY 2 cut(s) 272, 409
XspI CTAG 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.