Rroxscaffold_6G00393300

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000979
Physical Location & Seq
Reverse (-)
13359241 .. 13359659
419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393300.1

Sequence Viewer

Length: 318 bp
ATGGATGACATTGTTTCCACCAAGTTTGAGAAAGTGAGAGGACATGCACCTTCTAGCATTGATTGCTACATGAAGCAATTTGGAGTTTCAGAGCAAGAGACCGTCGACGTATTCAACAAAAAAGTTGCAGATTTGTGGAAGGATATAAATGAGGAGTTTCTTAGACCAACCGCTGTGCCAACGCCGGTTCTTGTGCATGTTCTCAATTTAACAAGAGTAACTGATCTCCTTTACAAAGGGGAAGATGGTTTCACACGGGTTGGGAAGATGACCAAAGATTGTGTTGCCGCGCTCCTTATCGATCCAATGCCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

105

Amino Acids

11.83

Weight (kDa)

5.28

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth_C PF03936 1 - 48 8.6e-12 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 1 - 48 1.1e-11 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AccII CGCG 1 cut(s) 290
AciI CCGC 2 cut(s) 171, 288
AclWI GGATC 1 cut(s) 296
AgsI TTSAA 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 292
BccI CCATC 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 54
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bsa29I ATCGAT 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 92
Bse118I RCCGGY 1 cut(s) 184
BseCI ATCGAT 1 cut(s) 300
BseGI GGATG 1 cut(s) 10
BseRI GAGGAG 1 cut(s) 167
Bsh1236I CGCG 1 cut(s) 290
BshVI ATCGAT 1 cut(s) 300
BsiSI CCGG 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 223, 301
BspACI CCGC 2 cut(s) 171, 288
BspDI ATCGAT 1 cut(s) 300
BspFNI CGCG 1 cut(s) 290
BspPI GGATC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 184
BssMI GATC 2 cut(s) 223, 301
Bst4CI ACNGT 2 cut(s) 103, 315
BstAPI GCANNNNNTGC 1 cut(s) 63
BstDEI CTNAG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 2 cut(s) 226, 304
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 2 cut(s) 223, 301
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNSI RCATGY 2 cut(s) 47, 200
BstUI CGCG 1 cut(s) 290
Bsu15I ATCGAT 1 cut(s) 300
BsuTUI ATCGAT 1 cut(s) 300
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 311
CfoI GCGC 1 cut(s) 292
Cfr10I RCCGGY 1 cut(s) 184
ClaI ATCGAT 1 cut(s) 300
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 3 cut(s) 44, 70, 197
DdeI CTNAG 1 cut(s) 161
DpnI GATC 2 cut(s) 225, 303
DpnII GATC 2 cut(s) 223, 301
Eco31I GGTCTC 1 cut(s) 92
FaeI CATG 3 cut(s) 47, 73, 200
FaiI YATR 4 cut(s) 45, 71, 146, 198
FatI CATG 3 cut(s) 43, 69, 196
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 54
GlaI GCGC 1 cut(s) 291
GluI GCNGC 1 cut(s) 288
HapII CCGG 1 cut(s) 185
HhaI GCGC 1 cut(s) 292
Hin1II CATG 3 cut(s) 47, 73, 200
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HpaII CCGG 1 cut(s) 185
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 91
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 2 cut(s) 107, 110
HpyAV CCTTC 2 cut(s) 60, 133
HpyCH4III ACNGT 2 cut(s) 103, 315
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 3 cut(s) 47, 128, 196
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 3 cut(s) 47, 73, 200
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 2 cut(s) 223, 301
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 1 cut(s) 198
MaeI CTAG 1 cut(s) 54
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 225, 303
MboI GATC 2 cut(s) 223, 301
MboII GAAGA 2 cut(s) 254, 277
MluCI AATT 2 cut(s) 77, 205
MnlI CCTC 2 cut(s) 32, 145
MseI TTAA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 2 cut(s) 223, 301
NlaIII CATG 3 cut(s) 47, 73, 200
NspI RCATGY 2 cut(s) 47, 200
PkrI GCNGC 1 cut(s) 289
SalI GTCGAC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 2 cut(s) 223, 301
SetI ASST 2 cut(s) 52, 111
SgrDI CGTCGACG 1 cut(s) 104
Sse9I AATT 2 cut(s) 77, 205
SsiI CCGC 2 cut(s) 171, 288
SspMI CTAG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 103, 315
TaiI ACGT 1 cut(s) 111
TaqI TCGA 2 cut(s) 105, 300
TasI AATT 2 cut(s) 77, 205
TauI GCSGC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 1 cut(s) 318
TspDTI ATGAA 1 cut(s) 86
TspRI CASTG 1 cut(s) 318
XceI RCATGY 2 cut(s) 47, 200
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.