Rh3DG328300

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000979
Physical Location & Seq
Reverse (-)
36104211 .. 36108911
4701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG328300.1

Sequence Viewer

Length: 369 bp
ATGACCCTAAAATTCTTAGAGCTTCCGATACGTACCATTTTTAGGCTCATGGATGACATTGTTTCCACCAAGTTTGAGATAGTGAGAGGACATGCACCTTCTAGCATTGATTGCTACATGACGCAATATGGAGTTTCAGATCAAGAGACCGTCGACGTATTCAACAAAAAAGTTCCAGATTTGTGGAAGGATATAAATGAGGAGTTTCTTAGACCAACCGCTGTGCCAACGCCGGTTCTTGTGCGTGTTCTCAATTTAACAAGAGTAACTGATCTCCTTTACAAAGGGGAAGATGGTTTCACACGGGTTGGGAAGATGACCAAAGATTGTGTTGCCGCGCTCCTTGTCGATCCAGTGCCACTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

122

Amino Acids

13.91

Weight (kDa)

5.35

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth_C PF03936 11 - 64 5.2e-14 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 11 - 64 6.3e-13 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 338
AciI CCGC 2 cut(s) 219, 336
AclWI GGATC 1 cut(s) 344
AcsI RAATTY 1 cut(s) 11
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 1 cut(s) 163
AluBI AGCT 1 cut(s) 22
AluI AGCT 1 cut(s) 22
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 1 cut(s) 344
ApoI RAATTY 1 cut(s) 11
AspLEI GCGC 1 cut(s) 340
BccI CCATC 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 1 cut(s) 102
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
BsaAI YACGTR 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 1 cut(s) 353
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 353
BseRI GAGGAG 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 140
Bsp143I GATC 3 cut(s) 139, 271, 349
BspACI CCGC 2 cut(s) 219, 336
BspFNI CGCG 1 cut(s) 338
BspPI GGATC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 1 cut(s) 353
BssAI RCCGGY 1 cut(s) 232
BssMI GATC 3 cut(s) 139, 271, 349
Bst4CI ACNGT 1 cut(s) 151
BstAPI GCANNNNNTGC 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 32
BstDEI CTNAG 2 cut(s) 16, 209
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 338
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 3 cut(s) 142, 274, 352
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 3 cut(s) 139, 271, 349
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 95
BstSNI TACGTA 1 cut(s) 32
BstUI CGCG 1 cut(s) 338
BstXI CCANNNNNNTGG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 360
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 232
CseI GACGC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 33
CspCI CAANNNNNGTGG 2 cut(s) 55, 90
CviAII CATG 3 cut(s) 49, 92, 118
CviJI RGCY 2 cut(s) 22, 46
CviKI_1 RGCY 2 cut(s) 22, 46
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 2 cut(s) 16, 209
DpnI GATC 3 cut(s) 141, 273, 351
DpnII GATC 3 cut(s) 139, 271, 349
Eco105I TACGTA 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 140
FaeI CATG 3 cut(s) 52, 95, 121
FaiI YATR 5 cut(s) 50, 93, 119, 129, 194
FatI CATG 3 cut(s) 48, 91, 117
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 336
FspBI CTAG 1 cut(s) 102
GlaI GCGC 1 cut(s) 339
GluI GCNGC 1 cut(s) 336
HapII CCGG 1 cut(s) 233
HgaI GACGC 1 cut(s) 130
HhaI GCGC 1 cut(s) 340
Hin1II CATG 3 cut(s) 52, 95, 121
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HpaII CCGG 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 27, 139
Hpy188III TCNNGA 2 cut(s) 143, 176
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 2 cut(s) 155, 158
HpyAV CCTTC 2 cut(s) 108, 181
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 2 cut(s) 31, 156
HpyCH4V TGCA 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 2 cut(s) 16, 209
HpySE526I ACGT 2 cut(s) 31, 156
Hsp92II CATG 3 cut(s) 52, 95, 121
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 3 cut(s) 139, 271, 349
LmnI GCTCC 1 cut(s) 345
LpnPI CCDG 2 cut(s) 189, 246
MaeI CTAG 1 cut(s) 102
MaeII ACGT 2 cut(s) 31, 156
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 141, 273, 351
MboI GATC 3 cut(s) 139, 271, 349
MboII GAAGA 2 cut(s) 302, 325
MluCI AATT 2 cut(s) 11, 253
MnlI CCTC 2 cut(s) 80, 193
MseI TTAA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 111
NdeII GATC 3 cut(s) 139, 271, 349
NlaIII CATG 3 cut(s) 52, 95, 121
NspI RCATGY 1 cut(s) 95
PkrI GCNGC 1 cut(s) 337
Ppu21I YACGTR 1 cut(s) 32
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 3 cut(s) 139, 271, 349
SetI ASST 4 cut(s) 24, 34, 100, 159
SgrDI CGTCGACG 1 cut(s) 152
SnaBI TACGTA 1 cut(s) 32
Sse9I AATT 2 cut(s) 11, 253
SsiI CCGC 2 cut(s) 219, 336
SspMI CTAG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 2 cut(s) 34, 159
TaqI TCGA 2 cut(s) 153, 348
TasI AATT 2 cut(s) 11, 253
TauI GCSGC 1 cut(s) 338
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 1 cut(s) 360
TspRI CASTG 1 cut(s) 360
XapI RAATTY 1 cut(s) 11
XceI RCATGY 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.