Prupe.4G194000_v2.0.a1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000979
Physical Location & Seq
Forward (+)
11697671 .. 11698758
1088 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G194000.1

Sequence Viewer

Length: 753 bp
ATGGATGATATATACGATGCATATGGTACCTTCGAAGAACTTGAGATCTTTACAGAAGCAATTGGGAGGTGGGATGTCAATTGCATGGATGAACTACCAAACTATATGCAAATATTTTATCAAACACTTCTGAATCTTTTCAATGAAATTGAGGAAGAGATGGTGAAGAAAGGGAATTCATGCCGAGTTTACTATGCAAAAGGAGCTTGGAAAGCTACAGCCCGAGCTTACTTTGATGAGGCCAGATGGTTACACGAAGGATGCATCCCAAGCATGGAGGAGTATATGCATGTTGCCACAGCTTCTGCTGGTAACAGCCCACTTTCAACCATATCTTTACTTGGCATGGGAGATATTGTAACCAAGGAGGCATTTGAGTGGTTGCTCAATAACCCTAAAATCCTTAGAGCTTCGAATATCATTTTTAGGCTCATGGATGACGTTGCTGGGTACAAGTTTGACAAGGAGAGAGGGCATGTTGCTTCTAGTATTGATTGCTACATGAAGCAACATGGAGTCTCAGAGCAAGAGACACTTGATGTTTTCAACAAACAAGTTGTGGATTTGTGGAAGGATATAAACGAGGAGCTCCTTATAAAGCCAACTGTTGTGCCAAGGCCTGTCCTCATGCGCGTTCTCAATTTAACAAGAGTTGTGGATCTGCTTTACAAAAGGGGAGATGGCTTCGCACATGTTGGGAAACTTATGAAAGATATTGTTGCTTCACTTTTTCTAGATCCTGTTCCACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

251

Amino Acids

28.68

Weight (kDa)

4.91

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
Acc65I GGTACC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 26
AccII CGCG 1 cut(s) 633
AclWI GGATC 2 cut(s) 666, 731
AcsI RAATTY 1 cut(s) 175
AfaI GTAC 2 cut(s) 28, 452
AfiI CCNNNNNNNGG 1 cut(s) 274
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 3 cut(s) 142, 327, 547
AluBI AGCT 6 cut(s) 206, 215, 227, 302, 410, 589
AluI AGCT 6 cut(s) 206, 215, 227, 302, 410, 589
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 523, 524
AlwI GGATC 2 cut(s) 666, 731
AlwNI CAGNNNCTG 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 222
AoxI GGCC 2 cut(s) 240, 617
ApoI RAATTY 1 cut(s) 175
Asp700I GAANNNNTTC 1 cut(s) 137
Asp718I GGTACC 1 cut(s) 26
AspLEI GCGC 1 cut(s) 633
AsuHPI GGTGA 1 cut(s) 175
AsuII TTCGAA 2 cut(s) 33, 413
AvaI CYCGRG 1 cut(s) 222
BanI GGYRCC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 591
BccI CCATC 3 cut(s) 154, 240, 674
BcoDI GTCTC 2 cut(s) 523, 524
BfaI CTAG 2 cut(s) 486, 734
BfmI CTRYAG 1 cut(s) 216
BglII AGATCT 1 cut(s) 45
BmeT110I CYCGRG 1 cut(s) 222
BmiI GGNNCC 1 cut(s) 28
BmsI GCATC 3 cut(s) 7, 251, 273
Bpu14I TTCGAA 2 cut(s) 33, 413
BpuEI CTTGAG 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 363, 614
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 363, 614
BseGI GGATG 6 cut(s) 10, 79, 94, 264, 266, 442
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 534
BseRI GAGGAG 2 cut(s) 293, 599
BseYI CCCAGC 1 cut(s) 446
Bsh1236I CGCG 1 cut(s) 633
BshFI GGCC 2 cut(s) 242, 619
BshNI GGYRCC 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 274
BsmAI GTCTC 2 cut(s) 523, 524
BsnI GGCC 2 cut(s) 242, 619
BsoBI CYCGRG 1 cut(s) 222
Bsp119I TTCGAA 2 cut(s) 33, 413
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 3 cut(s) 45, 658, 736
BspANI GGCC 2 cut(s) 242, 619
BspCNI CTCAG 1 cut(s) 533
BspFNI CGCG 1 cut(s) 633
BspLI GGNNCC 1 cut(s) 28
BspPI GGATC 2 cut(s) 666, 731
BspT104I TTCGAA 2 cut(s) 33, 413
BspT107I GGYRCC 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 363, 614
BssMI GATC 3 cut(s) 45, 658, 736
BssT1I CCWWGG 2 cut(s) 363, 614
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 1 cut(s) 150
BstBI TTCGAA 2 cut(s) 33, 413
BstDEI CTNAG 2 cut(s) 404, 520
BstF5I GGATG 6 cut(s) 10, 79, 94, 264, 266, 442
BstFNI CGCG 1 cut(s) 633
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 3 cut(s) 48, 661, 739
BstMAI GTCTC 2 cut(s) 523, 524
BstMBI GATC 3 cut(s) 45, 658, 736
BstMWI GCNNNNNNNGC 3 cut(s) 203, 212, 270
BstNSI RCATGY 3 cut(s) 293, 479, 695
BstSFI CTRYAG 1 cut(s) 216
BstUI CGCG 1 cut(s) 633
BstX2I RGATCY 3 cut(s) 45, 658, 736
BstYI RGATCY 3 cut(s) 45, 658, 736
BsuRI GGCC 2 cut(s) 242, 619
BtsCI GGATG 6 cut(s) 10, 79, 94, 264, 266, 442
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 1 cut(s) 633
Csp6I GTAC 2 cut(s) 27, 451
CspCI CAANNNNNGTGG 2 cut(s) 636, 671
CviQI GTAC 2 cut(s) 27, 451
DdeI CTNAG 2 cut(s) 404, 520
DpnI GATC 3 cut(s) 47, 660, 738
DpnII GATC 3 cut(s) 45, 658, 736
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Ecl136II GAGCTC 1 cut(s) 589
Eco130I CCWWGG 2 cut(s) 363, 614
Eco147I AGGCCT 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 591
Eco53kI GAGCTC 1 cut(s) 589
Eco88I CYCGRG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 589
EcoRI GAATTC 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 363, 614
EcoT22I ATGCAT 3 cut(s) 22, 266, 291
EcoT38I GRGCYC 1 cut(s) 591
ErhI CCWWGG 2 cut(s) 363, 614
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FauNDI CATATG 1 cut(s) 22
FokI GGATG 6 cut(s) 17, 86, 101, 251, 273, 449
FriOI GRGCYC 1 cut(s) 591
FspBI CTAG 2 cut(s) 486, 734
GlaI GCGC 1 cut(s) 632
GsaI CCCAGC 1 cut(s) 450
HaeIII GGCC 2 cut(s) 242, 619
HhaI GCGC 1 cut(s) 633
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HinfI GANTC 2 cut(s) 133, 516
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 2 cut(s) 132, 523
Hpy188III TCNNGA 1 cut(s) 734
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 3 cut(s) 40, 251, 565
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 6 cut(s) 20, 84, 109, 197, 264, 289
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 212, 270
HpyF3I CTNAG 2 cut(s) 404, 520
HpySE526I ACGT 1 cut(s) 441
HspAI GCGC 1 cut(s) 631
KpnI GGTACC 1 cut(s) 30
Kzo9I GATC 3 cut(s) 45, 658, 736
LmnI GCTCC 3 cut(s) 203, 586, 594
LpnPI CCDG 4 cut(s) 256, 294, 432, 633
LweI GCATC 3 cut(s) 7, 251, 273
MaeI CTAG 2 cut(s) 486, 734
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 3 cut(s) 249, 311, 358
MalI GATC 3 cut(s) 47, 660, 738
MboI GATC 3 cut(s) 45, 658, 736
MboII GAAGA 3 cut(s) 47, 167, 178
MfeI CAATTG 2 cut(s) 60, 79
MflI RGATCY 3 cut(s) 45, 658, 736
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 5 cut(s) 60, 79, 147, 175, 640
MlyI GAGTC 1 cut(s) 525
MnlI CCTC 8 cut(s) 60, 145, 232, 271, 361, 464, 577, 635
Mph1103I ATGCAT 3 cut(s) 22, 266, 291
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 2 cut(s) 644, 751
MslI CAYNNNNRTG 1 cut(s) 376
MunI CAATTG 2 cut(s) 60, 79
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 3 cut(s) 203, 212, 270
NdeI CATATG 1 cut(s) 22
NdeII GATC 3 cut(s) 45, 658, 736
NlaIV GGNNCC 1 cut(s) 28
NmeAIII GCCGAG 1 cut(s) 209
NsiI ATGCAT 3 cut(s) 22, 266, 291
NspI RCATGY 3 cut(s) 293, 479, 695
NspV TTCGAA 2 cut(s) 33, 413
PceI AGGCCT 1 cut(s) 619
PciI ACATGT 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 1 cut(s) 133
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
PscI ACATGT 1 cut(s) 691
PsiI TTATAA 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 591
PspFI CCCAGC 1 cut(s) 446
PspN4I GGNNCC 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 3 cut(s) 45, 658, 736
RsaI GTAC 2 cut(s) 28, 452
RsaNI GTAC 2 cut(s) 27, 451
RseI CAYNNNNRTG 1 cut(s) 376
SacI GAGCTC 1 cut(s) 591
SaqAI TTAA 2 cut(s) 644, 751
Sau3AI GATC 3 cut(s) 45, 658, 736
SchI GAGTC 1 cut(s) 525
SduI GDGCHC 1 cut(s) 591
SetI ASST 9 cut(s) 32, 71, 208, 217, 229, 304, 412, 444, 591
SfaNI GCATC 3 cut(s) 7, 251, 273
SfcI CTRYAG 1 cut(s) 216
SfuI TTCGAA 2 cut(s) 33, 413
SmiMI CAYNNNNRTG 1 cut(s) 376
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 5 cut(s) 60, 79, 147, 175, 640
SseBI AGGCCT 1 cut(s) 619
SspI AATATT 1 cut(s) 114
SspMI CTAG 2 cut(s) 486, 734
SstI GAGCTC 1 cut(s) 591
StuI AGGCCT 1 cut(s) 619
StyI CCWWGG 2 cut(s) 363, 614
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 1 cut(s) 444
TaqI TCGA 2 cut(s) 33, 413
TasI AATT 5 cut(s) 60, 79, 147, 175, 640
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 2 cut(s) 644, 751
Tru9I TTAA 2 cut(s) 644, 751
TspDTI ATGAA 5 cut(s) 105, 159, 168, 518, 722
XapI RAATTY 1 cut(s) 175
XbaI TCTAGA 1 cut(s) 733
XceI RCATGY 3 cut(s) 293, 479, 695
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 2 cut(s) 486, 734
Zsp2I ATGCAT 3 cut(s) 22, 266, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.