Rh1CG074300

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000979
Physical Location & Seq
Reverse (-)
15481272 .. 15497170
15899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG074300.1

Sequence Viewer

Length: 1212 bp
ATGCCGGCACTGGAGGAGGATTTGTCGATGAATAGTGCCTTAGAAGGTGAGGTAAATGTCAAGGAGGTGATGTGGAGGCTGCGGCGGCTTCGGTGGCCAAGTCAAACGGCAATTTGGTTGCGAAATTGCACTATGAAACTTATTGTTTGTTTGTACTATATCTGGAAATATCAAAGACAAGTTAAAGTAGAAGCTGTACGAGAGAATAACCGCACGTTGCGGGAAAGAAAAGCAGCCTCCTTGAAGACTTCAAAGTCCATAGACTCCGACTCGCTGACTCGATGGTGGTTCACCGTTCACTCCCTCACTACCTCTCTCTTTTTTCCGGCGACCTCTGTTCTCTACCAAACCTCTCTCCGTCCGCGAAAGATCGGCGACCTCTTTTTCCGCATTGGTTTGGAGTTTGATGTTTCGGCTGACGATGGCGAAGAGGCGGAAGCTGAGTGTTTCTGTGCTCTCGGCGTTTCTCAGTTTCGCCTTTTCGCCGGGTTGTTCTCCTATCTTCCGAAGGACGATGAGCATGACATGGATGTTGATACTGCAACTTCTGCTACTCAAAGTCCCCTAAGAAACTTGTTGGTGCTGATTTTTCTGATTGATCAGAAAAAATACAGTGAGGCTAAAGCTTGTGCCACAGCTGGCATTGCTCGACTAAATAACATGAATGGGGGAACTATTGATGTCCTGTCCTGGCATCAAGGCTTTACTTCTATTACTCTCTTAGCTATGAATATGAGGGTGATATCGCAGAAATTAGAGGGTGGACATGCTGCAGGATGGCAAGATGTGCAGCCTCATGATCCTCAAGTTCAAGATGCAGCCAACCATGCTGTGAAGAGCCTTAAGCAAAAGTCCAATTCATTGTTCCCTTATGAGCTTCAAGAGATTGCCCATGCTAAGGCAGAGAATGCTTCTCCGGAAAAATCAAAAGATGGAAAAGATGCTGAAACTGCAGAAGCTGGACCCTCCTCGAGCAAGGATGCTTCTTCTAAAAAATCAACCTGTGGAAATGACGTTGAAACTTCAGGAGCTGGACCATTAAGCAAGGATGTGTCCCCTCAAACTGAAGCAAGAGGTGTAAATGATGCTGAGTCATCAGGAGCTGGACCTTCCTCAAGCAAGGTTGCTTCTGTAGAAAATGATCCAAGTGGTGGAAGTGAGGTTGAAACTCTCCTCCTTAAGCAAGGTTTTTCTTCTATGCCCAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
PCI Rpn3_C

Protein Analysis

403

Amino Acids

44.29

Weight (kDa)

5.77

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 253
AccB7I CCANNNNNTGG 1 cut(s) 1151
AccII CGCG 1 cut(s) 364
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 7 cut(s) 82, 85, 211, 220, 362, 388, 434
AclWI GGATC 2 cut(s) 794, 1136
AcoI YGGCCR 1 cut(s) 95
AcuI CTGAAG 2 cut(s) 1008, 1086
AfaI GTAC 2 cut(s) 155, 198
AfiI CCNNNNNNNGG 2 cut(s) 898, 1151
AflII CTTAAG 2 cut(s) 842, 1178
AgsI TTSAA 6 cut(s) 244, 252, 812, 881, 1019, 1166
AjnI CCWGG 1 cut(s) 689
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AluBI AGCT 9 cut(s) 194, 440, 626, 638, 725, 877, 959, 1031, 1103
AluI AGCT 9 cut(s) 194, 440, 626, 638, 725, 877, 959, 1031, 1103
Alw21I GWGCWC 1 cut(s) 457
AlwI GGATC 2 cut(s) 794, 1136
AlwNI CAGNNNCTG 3 cut(s) 959, 1031, 1103
Ama87I CYCGRG 1 cut(s) 970
Aor13HI TCCGGA 1 cut(s) 916
AoxI GGCC 1 cut(s) 95
ApeKI GCWGC 5 cut(s) 79, 233, 770, 790, 818
AspS9I GGNCC 3 cut(s) 962, 1034, 1106
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 4 cut(s) 59, 79, 283, 751
AvaI CYCGRG 1 cut(s) 970
AvaII GGWCC 3 cut(s) 962, 1034, 1106
BalI TGGCCA 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 5 cut(s) 66, 245, 757, 802, 830
BccI CCATC 4 cut(s) 276, 416, 771, 926
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 1 cut(s) 691
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 1 cut(s) 487
BfmI CTRYAG 3 cut(s) 771, 951, 1131
BfrI CTTAAG 2 cut(s) 842, 1178
BisI GCNGC 7 cut(s) 80, 83, 86, 234, 771, 791, 819
BlsI GCNGC 7 cut(s) 81, 84, 87, 235, 772, 792, 820
Bme1390I CCNGG 2 cut(s) 487, 691
Bme18I GGWCC 3 cut(s) 962, 1034, 1106
BmeT110I CYCGRG 1 cut(s) 970
BmgT120I GGNCC 3 cut(s) 962, 1034, 1106
BmiI GGNNCC 1 cut(s) 964
BmrFI CCNGG 2 cut(s) 487, 691
BmsI GCATC 5 cut(s) 703, 805, 931, 970, 1075
BpiI GAAGAC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 897
BpuEI CTTGAG 2 cut(s) 789, 1099
BpuMI CCSGG 1 cut(s) 487
BsaWI WCCGGW 1 cut(s) 916
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 56, 86
Bsc4I CCNNNNNNNGG 2 cut(s) 898, 1151
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 916
BseBI CCWGG 1 cut(s) 691
BseGI GGATG 4 cut(s) 535, 782, 985, 1054
BseLI CCNNNNNNNGG 2 cut(s) 898, 1151
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 3 cut(s) 432, 482, 1080
BseNI ACTGG 1 cut(s) 15
BseRI GAGGAG 3 cut(s) 29, 958, 1163
BseXI GCAGC 5 cut(s) 66, 245, 757, 802, 830
BsgI GTGCAG 1 cut(s) 809
Bsh1236I CGCG 1 cut(s) 364
BshFI GGCC 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 970
BsiSI CCGG 4 cut(s) 5, 326, 486, 917
BslFI GGGAC 2 cut(s) 546, 1039
BslI CCNNNNNNNGG 2 cut(s) 898, 1151
BsmFI GGGAC 2 cut(s) 546, 1039
BsmI GAATGC 1 cut(s) 913
BsnI GGCC 1 cut(s) 97
BsoBI CYCGRG 1 cut(s) 970
Bsp1286I GDGCHC 1 cut(s) 457
Bsp13I TCCGGA 1 cut(s) 916
Bsp143I GATC 4 cut(s) 369, 598, 799, 1141
BspACI CCGC 7 cut(s) 82, 85, 211, 220, 362, 388, 434
BspANI GGCC 1 cut(s) 97
BspCNI CTCAG 3 cut(s) 433, 481, 1081
BspEI TCCGGA 1 cut(s) 916
BspFNI CGCG 1 cut(s) 364
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 1 cut(s) 964
BspMAI CTGCAG 2 cut(s) 775, 955
BspPI GGATC 2 cut(s) 794, 1136
BspQI GCTCTTC 1 cut(s) 830
BspTI CTTAAG 2 cut(s) 842, 1178
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 1 cut(s) 15
BssAI RCCGGY 1 cut(s) 4
BssMI GATC 4 cut(s) 369, 598, 799, 1141
Bst2UI CCWGG 1 cut(s) 691
Bst4CI ACNGT 2 cut(s) 295, 614
Bst6I CTCTTC 2 cut(s) 423, 830
BstAFI CTTAAG 2 cut(s) 842, 1178
BstAPI GCANNNNNTGC 3 cut(s) 548, 787, 908
BstC8I GCNNGC 2 cut(s) 6, 640
BstDEI CTNAG 7 cut(s) 40, 441, 468, 566, 721, 897, 1089
BstF5I GGATG 4 cut(s) 535, 782, 985, 1054
BstFNI CGCG 1 cut(s) 364
BstKTI GATC 4 cut(s) 372, 601, 802, 1144
BstMBI GATC 4 cut(s) 369, 598, 799, 1141
BstMWI GCNNNNNNNGC 8 cut(s) 85, 94, 548, 644, 787, 827, 908, 950
BstNI CCWGG 1 cut(s) 691
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 2 cut(s) 485, 689
BstSFI CTRYAG 3 cut(s) 771, 951, 1131
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 5 cut(s) 66, 245, 757, 802, 830
BstV2I GAAGAC 1 cut(s) 251
BsuRI GGCC 1 cut(s) 97
BtsCI GGATG 4 cut(s) 535, 782, 985, 1054
BtsIMutI CAGTG 2 cut(s) 8, 619
Cac8I GCNNGC 2 cut(s) 6, 640
CaiI CAGNNNCTG 3 cut(s) 959, 1031, 1103
CciI TCATGA 1 cut(s) 796
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 3 cut(s) 962, 1034, 1106
Csp6I GTAC 2 cut(s) 154, 197
CviAII CATG 7 cut(s) 521, 526, 661, 767, 797, 827, 893
CviQI GTAC 2 cut(s) 154, 197
DdeI CTNAG 7 cut(s) 40, 441, 468, 566, 721, 897, 1089
DpnI GATC 4 cut(s) 371, 600, 801, 1143
DpnII GATC 4 cut(s) 369, 598, 799, 1141
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 423, 830
EarI CTCTTC 2 cut(s) 423, 830
EciI GGCGGA 1 cut(s) 449
Eco32I GATATC 1 cut(s) 744
Eco47I GGWCC 3 cut(s) 962, 1034, 1106
Eco57I CTGAAG 2 cut(s) 1008, 1086
Eco88I CYCGRG 1 cut(s) 970
EcoRII CCWGG 1 cut(s) 689
EcoRV GATATC 1 cut(s) 744
FaeI CATG 7 cut(s) 524, 529, 664, 770, 800, 830, 896
FaqI GGGAC 2 cut(s) 546, 1039
FatI CATG 7 cut(s) 520, 525, 660, 766, 796, 826, 892
FauI CCCGC 1 cut(s) 213
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 7 cut(s) 80, 83, 86, 234, 771, 791, 819
FokI GGATG 4 cut(s) 542, 789, 992, 1061
Fsp4HI GCNGC 7 cut(s) 80, 83, 86, 234, 771, 791, 819
GluI GCNGC 7 cut(s) 80, 83, 86, 234, 771, 791, 819
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 1 cut(s) 97
HapII CCGG 4 cut(s) 5, 326, 486, 917
Hin1II CATG 7 cut(s) 524, 529, 664, 770, 800, 830, 896
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 4 cut(s) 263, 269, 277, 1091
HpaII CCGG 4 cut(s) 5, 326, 486, 917
HphI GGTGA 4 cut(s) 59, 79, 283, 751
Hpy166II GTNNAC 3 cut(s) 291, 298, 764
Hpy188I TCNGA 4 cut(s) 268, 507, 594, 603
Hpy188III TCNNGA 7 cut(s) 163, 797, 812, 881, 917, 1026, 1098
Hpy8I GTNNAC 3 cut(s) 291, 298, 764
HpyAV CCTTC 3 cut(s) 38, 502, 1119
HpyCH4III ACNGT 2 cut(s) 295, 614
HpyCH4IV ACGT 2 cut(s) 215, 1014
HpyCH4V TGCA 6 cut(s) 129, 542, 773, 790, 818, 953
HpyF10VI GCNNNNNNNGC 8 cut(s) 85, 94, 548, 644, 787, 827, 908, 950
HpyF3I CTNAG 7 cut(s) 40, 441, 468, 566, 721, 897, 1089
HpySE526I ACGT 2 cut(s) 215, 1014
Hsp92II CATG 7 cut(s) 524, 529, 664, 770, 800, 830, 896
Kpn2I TCCGGA 1 cut(s) 916
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 4 cut(s) 369, 598, 799, 1141
LguI GCTCTTC 1 cut(s) 830
LmnI GCTCC 2 cut(s) 1028, 1100
Lsp1109I GCAGC 5 cut(s) 66, 245, 757, 802, 830
LweI GCATC 5 cut(s) 703, 805, 931, 970, 1075
MaeII ACGT 2 cut(s) 215, 1014
MalI GATC 4 cut(s) 371, 600, 801, 1143
MboI GATC 4 cut(s) 369, 598, 799, 1141
MboII GAAGA 6 cut(s) 256, 440, 494, 847, 978, 1185
MhlI GDGCHC 1 cut(s) 457
MlsI TGGCCA 1 cut(s) 97
MluCI AATT 4 cut(s) 111, 124, 752, 856
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 4 cut(s) 257, 263, 271, 1100
MmeI TCCRAC 1 cut(s) 291
Mox20I TGGCCA 1 cut(s) 97
MroI TCCGGA 1 cut(s) 916
MroNI GCCGGC 1 cut(s) 4
MscI TGGCCA 1 cut(s) 97
MseI TTAA 4 cut(s) 183, 843, 1040, 1179
Msp20I TGGCCA 1 cut(s) 97
MspA1I CMGCKG 1 cut(s) 638
MspCI CTTAAG 2 cut(s) 842, 1178
MspI CCGG 4 cut(s) 5, 326, 486, 917
MspR9I CCNGG 2 cut(s) 487, 691
Mva1269I GAATGC 1 cut(s) 913
MvaI CCWGG 1 cut(s) 691
MvnI CGCG 1 cut(s) 364
MwoI GCNNNNNNNGC 8 cut(s) 85, 94, 548, 644, 787, 827, 908, 950
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 487
NdeII GATC 4 cut(s) 369, 598, 799, 1141
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 7 cut(s) 524, 529, 664, 770, 800, 830, 896
NlaIV GGNNCC 1 cut(s) 964
NmeAIII GCCGAG 1 cut(s) 438
NspI RCATGY 1 cut(s) 770
PaeR7I CTCGAG 1 cut(s) 970
PagI TCATGA 1 cut(s) 796
PciSI GCTCTTC 1 cut(s) 830
PctI GAATGC 1 cut(s) 913
PdiI GCCGGC 1 cut(s) 6
PflMI CCANNNNNTGG 1 cut(s) 1151
PkrI GCNGC 7 cut(s) 81, 84, 87, 235, 772, 792, 820
PleI GAGTC 4 cut(s) 257, 263, 271, 1099
PpsI GAGTC 4 cut(s) 257, 263, 271, 1099
Psp6I CCWGG 1 cut(s) 689
PspGI CCWGG 1 cut(s) 689
PspN4I GGNNCC 1 cut(s) 964
PspPI GGNCC 3 cut(s) 962, 1034, 1106
PspXI VCTCGAGB 1 cut(s) 970
PstI CTGCAG 2 cut(s) 775, 955
PstNI CAGNNNCTG 3 cut(s) 959, 1031, 1103
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 2 cut(s) 155, 198
RsaNI GTAC 2 cut(s) 154, 197
SapI GCTCTTC 1 cut(s) 830
SaqAI TTAA 4 cut(s) 183, 843, 1040, 1179
SatI GCNGC 7 cut(s) 80, 83, 86, 234, 771, 791, 819
Sau3AI GATC 4 cut(s) 369, 598, 799, 1141
Sau96I GGNCC 3 cut(s) 962, 1034, 1106
SchI GAGTC 4 cut(s) 257, 263, 271, 1100
ScrFI CCNGG 2 cut(s) 487, 691
SduI GDGCHC 1 cut(s) 457
SfaNI GCATC 5 cut(s) 703, 805, 931, 970, 1075
SfcI CTRYAG 3 cut(s) 771, 951, 1131
Sfr274I CTCGAG 1 cut(s) 970
SinI GGWCC 3 cut(s) 962, 1034, 1106
SlaI CTCGAG 1 cut(s) 970
SmlI CTYRAG 5 cut(s) 804, 842, 970, 1114, 1178
SmoI CTYRAG 5 cut(s) 804, 842, 970, 1114, 1178
Sse9I AATT 4 cut(s) 111, 124, 752, 856
SsiI CCGC 7 cut(s) 82, 85, 211, 220, 362, 388, 434
StyD4I CCNGG 2 cut(s) 485, 689
TaaI ACNGT 2 cut(s) 295, 614
TaiI ACGT 2 cut(s) 218, 1017
TaqI TCGA 4 cut(s) 26, 280, 649, 971
TasI AATT 4 cut(s) 111, 124, 752, 856
TatI WGTACW 1 cut(s) 153
TauI GCSGC 2 cut(s) 85, 88
Tru1I TTAA 4 cut(s) 183, 843, 1040, 1179
Tru9I TTAA 4 cut(s) 183, 843, 1040, 1179
TscAI CASTG 2 cut(s) 15, 619
TseI GCWGC 5 cut(s) 79, 233, 770, 790, 818
TspDTI ATGAA 5 cut(s) 44, 149, 677, 743, 849
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 2 cut(s) 15, 619
Van91I CCANNNNNTGG 1 cut(s) 1151
Vha464I CTTAAG 2 cut(s) 842, 1178
VpaK11BI GGWCC 3 cut(s) 962, 1034, 1106
XceI RCATGY 1 cut(s) 770
XhoI CTCGAG 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.