FvH4_5g12200

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001041
Physical Location & Seq
Forward (+)
6866702 .. 6868285
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12200.t1

Sequence Viewer

Length: 1137 bp
ATGGGTGTGCAACCCAAAAAGAAATCAGGGATATTCACAACGTTAAGCGATGATTTACTTGGCCAAGTTGTTAACAAGGTCAAGGACAAAGAAGATAGAAAGTCGTTTTCCGAGGTCTGCAAGCAATGGTTCAAAGTGGAAGGTCTAGACCGATCATCACTTATCCTTAATTTTTCCAAGCCTGGGTTTTCGCTTCCTGTACCATCTAGATTCCCAAATCTAATCAGTTTGGCAGTATGGGATTGCAAGACCCACACTGACCTTGAATTCATAGCCCAAAATTGTCCCAAACTCGAGAGCATCATGATTGAATGCAGAGAGGAGAAGCAAAACGGTGATGATGGTGGCATTTTGAGCCCGAAAGGACTACGTGCTTTGGGAGAAGGGTGTCCCAGATTATCCAAAGTTGAGATTTACGGGCCTAATGCAGTAGGGGATTCTGGAGTTGCTGAGCTTTTACATTCAACACATAACTTGGAGACCCTAGTTTTTTTAAACAATCAGTTGATATCAGTAGCAGCCCTCAGAGCAATTGGGTCCACATCTTCTATTAGCTCTTTAGAGTTAATGTTTTGTTATAACATTACTGATGAGGGATTGGCTTTTTTGGCGAATGGATCTATCTCAAAGACCCTCAAGGAATTGATCATTGAGACTGGCACTAAAATCACTGATGCTGGGGTCGACCTTTTGCGCAAAATGTGTAGCCTGGAGCACCTGGAATTGTCCTGCGAGGGAGAAAGCAACAAAATCACTGATATTGGAGGTGTGGGAATCTCTGCAATTCGAACCCTCAAGGAATTAAGATTGGATAATGTGGACGTGTCAGACCCTACCATGGTCGCGCTTGCACAGAATTGTCGCAACTTGGAGACACTTGATATAGGGGGTTGTGAAAAGGTAACTGGAGCTGGCATTTGTGCATTTTGCAATCACAAGTGCTTGAAGAGGCTTAATTTGGACAAGTTAAAGATCAGTCTTTCTGATGTGGAACGGCTAGTGCTTGGATGCCCGTCCTTGAAATCTGTTGTGGTGGAATCACGCAGGAGGCTTGATCCGACTTGGAGCAAGAAATTGATGCAAGACAGAACTAGAAAAGTTGTTAAATTTGTTGAAAGTTATGCTCTTGATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

379

Amino Acids

41.51

Weight (kDa)

6.94

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 13 - 52 2.1e-09 F-box
DUF7885 PF25372 122 - 261 4.8e-09 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370 PF24758 261 - 348 1.9e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
Acc16I TGCGCA 1 cut(s) 695
AccI GTMKAC 1 cut(s) 684
AccII CGCG 1 cut(s) 845
AclI AACGTT 1 cut(s) 41
AclWI GGATC 2 cut(s) 625, 1049
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 2 cut(s) 266, 1106
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 2 cut(s) 183, 838
AflIII ACRYGT 1 cut(s) 822
AgsI TTSAA 7 cut(s) 133, 266, 311, 465, 946, 1021, 1115
AjiI CACGTC 1 cut(s) 823
AjnI CCWGG 3 cut(s) 181, 708, 717
AluBI AGCT 3 cut(s) 454, 555, 911
AluI AGCT 3 cut(s) 454, 555, 911
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 3 cut(s) 473, 647, 866
AlwI GGATC 2 cut(s) 625, 1049
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 2 cut(s) 61, 419
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 2 cut(s) 266, 1106
AspLEI GCGC 2 cut(s) 696, 847
AspS9I GGNCC 2 cut(s) 419, 537
AsuHPI GGTGA 1 cut(s) 347
AsuII TTCGAA 1 cut(s) 787
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 537
BalI TGGCCA 1 cut(s) 63
BanII GRGCYC 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 1 cut(s) 530
BccI CCATC 2 cut(s) 211, 335
BceAI ACGGC 1 cut(s) 1010
BciT130I CCWGG 3 cut(s) 183, 710, 719
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 3 cut(s) 473, 647, 866
BfaI CTAG 5 cut(s) 146, 207, 485, 998, 1092
BisI GCNGC 1 cut(s) 519
BlpI GCTNAGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 3 cut(s) 183, 710, 719
Bme18I GGWCC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 293
BmgBI CACGTC 1 cut(s) 823
BmgT120I GGNCC 2 cut(s) 419, 537
BmiI GGNNCC 1 cut(s) 538
BmrFI CCNGG 3 cut(s) 183, 710, 719
BmsI GCATC 4 cut(s) 309, 664, 998, 1068
BpmI CTGGAG 3 cut(s) 462, 731, 927
Bpu1102I GCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 787
BpuEI CTTGAG 2 cut(s) 620, 779
BsaAI YACGTR 1 cut(s) 371
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 3 cut(s) 111, 182, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 838
Bse1I ACTGG 2 cut(s) 661, 910
Bse3DI GCAATG 1 cut(s) 131
BseBI CCWGG 3 cut(s) 183, 710, 719
BseDI CCNNGG 3 cut(s) 111, 182, 837
BseGI GGATG 1 cut(s) 1013
BseLI CCNNNNNNNGG 2 cut(s) 183, 838
BseMI GCAATG 1 cut(s) 131
BseMII CTCAG 2 cut(s) 441, 538
BseNI ACTGG 2 cut(s) 661, 910
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 1 cut(s) 530
BseYI CCCAGC 1 cut(s) 677
Bsh1236I CGCG 1 cut(s) 845
BshFI GGCC 2 cut(s) 63, 421
BsiHKAI GWGCWC 1 cut(s) 717
BsiHKCI CYCGRG 1 cut(s) 293
BslFI GGGAC 2 cut(s) 270, 375
BslI CCNNNNNNNGG 2 cut(s) 183, 838
BsmAI GTCTC 3 cut(s) 473, 647, 866
BsmFI GGGAC 2 cut(s) 270, 375
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 2 cut(s) 63, 421
Bso31I GGTCTC 1 cut(s) 473
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 787
Bsp1286I GDGCHC 2 cut(s) 359, 717
Bsp143I GATC 5 cut(s) 152, 617, 645, 972, 1054
Bsp1720I GCTNAGC 1 cut(s) 450
Bsp19I CCATGG 1 cut(s) 837
BspANI GGCC 2 cut(s) 63, 421
BspCNI CTCAG 2 cut(s) 442, 537
BspFNI CGCG 1 cut(s) 845
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 538
BspPI GGATC 2 cut(s) 625, 1049
BspT104I TTCGAA 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 131
BsrI ACTGG 2 cut(s) 661, 910
BssECI CCNNGG 3 cut(s) 111, 182, 837
BssMI GATC 5 cut(s) 152, 617, 645, 972, 1054
BssT1I CCWWGG 1 cut(s) 837
Bst2UI CCWGG 3 cut(s) 183, 710, 719
Bst4CI ACNGT 1 cut(s) 335
Bst6I CTCTTC 1 cut(s) 941
BstBAI YACGTR 1 cut(s) 371
BstBI TTCGAA 1 cut(s) 787
BstC8I GCNNGC 3 cut(s) 122, 849, 913
BstDEI CTNAG 2 cut(s) 450, 524
BstDSI CCRYGG 1 cut(s) 837
BstF5I GGATG 1 cut(s) 1013
BstFNI CGCG 1 cut(s) 845
BstHHI GCGC 2 cut(s) 696, 847
BstKTI GATC 5 cut(s) 155, 620, 648, 975, 1057
BstMAI GTCTC 3 cut(s) 473, 647, 866
BstMBI GATC 5 cut(s) 152, 617, 645, 972, 1054
BstMWI GCNNNNNNNGC 3 cut(s) 354, 527, 608
BstNI CCWGG 3 cut(s) 183, 710, 719
BstSCI CCNGG 3 cut(s) 181, 708, 717
BstUI CGCG 1 cut(s) 845
BstV1I GCAGC 1 cut(s) 530
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
BsuRI GGCC 2 cut(s) 63, 421
BtgI CCRYGG 1 cut(s) 837
BtgZI GCGATG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 823
BtsCI GGATG 1 cut(s) 1013
BtsIMutI CAGTG 3 cut(s) 255, 669, 753
Cac8I GCNNGC 3 cut(s) 122, 849, 913
CciI TCATGA 1 cut(s) 303
CfoI GCGC 2 cut(s) 696, 847
Cfr13I GGNCC 2 cut(s) 419, 537
Csp6I GTAC 1 cut(s) 200
CviAII CATG 2 cut(s) 304, 838
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 2 cut(s) 450, 524
DpnI GATC 5 cut(s) 154, 619, 647, 974, 1056
DpnII GATC 5 cut(s) 152, 617, 645, 972, 1054
DraI TTTAAA 1 cut(s) 495
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 941
EarI CTCTTC 1 cut(s) 941
Eco130I CCWWGG 1 cut(s) 837
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 473
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 537
Eco88I CYCGRG 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 181, 708, 717
EcoRV GATATC 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 837
EcoT38I GRGCYC 1 cut(s) 359
ErhI CCWWGG 1 cut(s) 837
FaeI CATG 2 cut(s) 307, 841
FaiI YATR 9 cut(s) 238, 272, 305, 471, 579, 839, 884, 1122, 1133
FaqI GGGAC 2 cut(s) 270, 375
FatI CATG 2 cut(s) 303, 837
FbaI TGATCA 1 cut(s) 645
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 1 cut(s) 1020
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 1 cut(s) 519
FspBI CTAG 5 cut(s) 146, 207, 485, 998, 1092
FspI TGCGCA 1 cut(s) 695
GlaI GCGC 2 cut(s) 695, 846
GluI GCNGC 1 cut(s) 519
GsaI CCCAGC 1 cut(s) 681
GsuI CTGGAG 3 cut(s) 462, 731, 927
HaeIII GGCC 2 cut(s) 63, 421
HhaI GCGC 2 cut(s) 696, 847
Hin1II CATG 2 cut(s) 307, 841
Hin6I GCGC 2 cut(s) 694, 845
HinP1I GCGC 2 cut(s) 694, 845
HincII GTYRAC 2 cut(s) 73, 685
HindII GTYRAC 2 cut(s) 73, 685
HinfI GANTC 4 cut(s) 210, 437, 774, 1037
HpaI GTTAAC 1 cut(s) 73
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 4 cut(s) 73, 540, 685, 820
Hpy188I TCNGA 5 cut(s) 112, 527, 829, 985, 1059
Hpy188III TCNNGA 6 cut(s) 146, 207, 295, 304, 441, 1127
Hpy8I GTNNAC 4 cut(s) 73, 540, 685, 820
HpyAV CCTTC 2 cut(s) 134, 377
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4IV ACGT 3 cut(s) 41, 370, 822
HpyF10VI GCNNNNNNNGC 3 cut(s) 354, 527, 608
HpyF3I CTNAG 2 cut(s) 450, 524
HpySE526I ACGT 3 cut(s) 41, 370, 822
Hsp92II CATG 2 cut(s) 307, 841
HspAI GCGC 2 cut(s) 694, 845
Ksp22I TGATCA 1 cut(s) 645
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 5 cut(s) 152, 617, 645, 972, 1054
LmnI GCTCC 3 cut(s) 712, 908, 1065
Lsp1109I GCAGC 1 cut(s) 530
LweI GCATC 4 cut(s) 309, 664, 998, 1068
MaeI CTAG 5 cut(s) 146, 207, 485, 998, 1092
MaeII ACGT 3 cut(s) 41, 370, 822
MaeIII GTNAC 1 cut(s) 901
MalI GATC 5 cut(s) 154, 619, 647, 974, 1056
MboI GATC 5 cut(s) 152, 617, 645, 972, 1054
MboII GAAGA 3 cut(s) 104, 537, 958
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 2 cut(s) 359, 717
MlsI TGGCCA 1 cut(s) 63
MluNI TGGCCA 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 1082
Mox20I TGGCCA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 63
MseI TTAA 9 cut(s) 44, 72, 168, 494, 566, 803, 954, 968, 1104
Msp20I TGGCCA 1 cut(s) 63
MspR9I CCNGG 3 cut(s) 183, 710, 719
MunI CAATTG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 3 cut(s) 183, 710, 719
MvnI CGCG 1 cut(s) 845
MwoI GCNNNNNNNGC 3 cut(s) 354, 527, 608
NcoI CCATGG 1 cut(s) 837
NdeII GATC 5 cut(s) 152, 617, 645, 972, 1054
NlaIII CATG 2 cut(s) 307, 841
NlaIV GGNNCC 1 cut(s) 538
NsbI TGCGCA 1 cut(s) 695
NspV TTCGAA 1 cut(s) 787
PaeR7I CTCGAG 1 cut(s) 293
PagI TCATGA 1 cut(s) 303
PctI GAATGC 1 cut(s) 317
PfeI GAWTC 4 cut(s) 210, 437, 774, 1037
PkrI GCNGC 1 cut(s) 520
Ppu21I YACGTR 1 cut(s) 371
PsiI TTATAA 1 cut(s) 579
Psp1406I AACGTT 1 cut(s) 41
Psp6I CCWGG 3 cut(s) 181, 708, 717
PspFI CCCAGC 1 cut(s) 677
PspGI CCWGG 3 cut(s) 181, 708, 717
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 2 cut(s) 419, 537
PsuI RGATCY 1 cut(s) 617
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SalI GTCGAC 1 cut(s) 683
SaqAI TTAA 9 cut(s) 44, 72, 168, 494, 566, 803, 954, 968, 1104
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 5 cut(s) 152, 617, 645, 972, 1054
Sau96I GGNCC 2 cut(s) 419, 537
ScrFI CCNGG 3 cut(s) 183, 710, 719
SduI GDGCHC 2 cut(s) 359, 717
SfaNI GCATC 4 cut(s) 309, 664, 998, 1068
Sfr274I CTCGAG 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 787
SinI GGWCC 1 cut(s) 537
SlaI CTCGAG 1 cut(s) 293
SmlI CTYRAG 3 cut(s) 293, 635, 794
SmoI CTYRAG 3 cut(s) 293, 635, 794
SspMI CTAG 5 cut(s) 146, 207, 485, 998, 1092
StyD4I CCNGG 3 cut(s) 181, 708, 717
StyI CCWWGG 1 cut(s) 837
TaaI ACNGT 1 cut(s) 335
TaiI ACGT 3 cut(s) 44, 373, 825
TaqI TCGA 3 cut(s) 294, 684, 787
TaqII GACCGA 1 cut(s) 165
TfiI GAWTC 4 cut(s) 210, 437, 774, 1037
Tru1I TTAA 9 cut(s) 44, 72, 168, 494, 566, 803, 954, 968, 1104
Tru9I TTAA 9 cut(s) 44, 72, 168, 494, 566, 803, 954, 968, 1104
TscAI CASTG 3 cut(s) 262, 676, 760
TseI GCWGC 1 cut(s) 518
TspDTI ATGAA 1 cut(s) 259
TspRI CASTG 3 cut(s) 262, 676, 760
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 2 cut(s) 266, 1106
XbaI TCTAGA 2 cut(s) 145, 206
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 684
XspI CTAG 5 cut(s) 146, 207, 485, 998, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.