Rh7AG074600

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001041
Physical Location & Seq
Reverse (-)
5316998 .. 5320405
3408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG074600.1

Sequence Viewer

Length: 960 bp
ATGATCATGGTGAATGTGAAGAACCCTATCCTCATTAACCAGAACTACTGCGACAAAGGCAAGTCGTGTAAGCTAATGATTCCATCCAAAGTACAGATCAGTGATGTGAGGTTCAAGAATATCAGGGGCTCGTCTTCATCTCCGGTAGCCGTCAAGCTGGACTGTAGTCACAGCGCGCCGTGCCGGAATGTGGAGCTGTCCGATATTGATCTCACGTACAGTGGTGACGAAAAAGACAAAATCACGTCCGAGTGTAATAATGTCAAGCCCAGACTTACTCGCGTGACTAAGGCTCTTGCTTGTGCTAATCTAGGTGACATACCAATAGATAATTCACCGCCTCATGATGATGGTGGTTTTGGACCAACATCCTCCGCCGCCGGCAATAGTTTGGGGCAAAACCCGACGGTCCGGGTGATGGTCGAAGGGTCAACAAAAACTCAACCCTTGCCTCTCGGCCCTCTCTTCTTCCTCTCCCCACAACATCCTCGCTGCTTCAAGAAGAAAACCCTCGCATCGATGATGCCACCGGCGAAGATCATCAGATCACTGACATTGGAGGTGTGGCGATCCTCTGCGATTCAGACCCTCAAAGAATTGAGACTGTGTCTTGAGCGCATCTCAGACGCCACCGTTGTTGCTCTTGCTGAGAATTGCATCAACTTGGAGATATTTGATTTAAACGGTTGTCATGAGGTGAGGGGAGATGGCATTCGTGCATTTTCAAGTCACAAGTGCTTGAAACGCCTTGTATTGCTCGGTTTGGGGGATTTTGATCTTTCGGATGTGGAAAGCATAGCGCTTGGATGGCCGTTATTGGAGTCTGTTGTGGTGGATTTGAGCTTGAGGCTGGATCCAGCTTGGAGCTCTGAATTGATGCATGACCGCACTAAAAGTGTCATCAAGTTTTCTCAATTCATTTTCAGTTGCTTTGCTCGCAAGATTGAGCATTTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

319

Amino Acids

35.13

Weight (kDa)

8.56

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 2 - 89 1.2e-20 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 176, 282
AciI CCGC 4 cut(s) 338, 375, 378, 886
AclWI GGATC 3 cut(s) 564, 848, 861
AcoI YGGCCR 1 cut(s) 809
AcyI GRCGYC 1 cut(s) 627
AfaI GTAC 2 cut(s) 93, 218
AfeI AGCGCT 1 cut(s) 801
AfiI CCNNNNNNNGG 2 cut(s) 190, 418
AgsI TTSAA 4 cut(s) 115, 499, 726, 742
AjiI CACGTC 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 316, 348
AluBI AGCT 6 cut(s) 73, 157, 196, 843, 860, 867
AluI AGCT 6 cut(s) 73, 157, 196, 843, 860, 867
Alw21I GWGCWC 1 cut(s) 869
Alw26I GTCTC 1 cut(s) 595
AlwI GGATC 3 cut(s) 564, 848, 861
Aor51HI AGCGCT 1 cut(s) 801
AoxI GGCC 2 cut(s) 457, 809
ApeKI GCWGC 1 cut(s) 492
AspLEI GCGC 4 cut(s) 176, 178, 618, 802
AspS9I GGNCC 3 cut(s) 362, 409, 458
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 6 cut(s) 22, 236, 326, 327, 427, 709
AvaII GGWCC 2 cut(s) 362, 409
BamHI GGATCC 1 cut(s) 853
BanII GRGCYC 2 cut(s) 131, 869
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 869
BbvI GCAGC 1 cut(s) 479
BccI CCATC 5 cut(s) 91, 344, 412, 701, 801
BceAI ACGGC 3 cut(s) 134, 163, 796
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 595
BfaI CTAG 2 cut(s) 311, 958
BfmI CTRYAG 1 cut(s) 163
BfoI RGCGCY 1 cut(s) 803
BisI GCNGC 2 cut(s) 378, 493
BlsI GCNGC 2 cut(s) 379, 494
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 2 cut(s) 362, 409
BmgBI CACGTC 1 cut(s) 246
BmgT120I GGNCC 3 cut(s) 362, 409, 458
BmiI GGNNCC 1 cut(s) 855
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 5 cut(s) 513, 524, 627, 666, 867
BoxI GACNNNNGTC 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpuEI CTTGAG 2 cut(s) 632, 865
BpuMI CCSGG 1 cut(s) 413
Bsa29I ATCGAT 1 cut(s) 518
BsaAI YACGTR 1 cut(s) 216
BsaBI GATNNNNATC 2 cut(s) 207, 774
BsaHI GRCGYC 1 cut(s) 627
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 418
Bse118I RCCGGY 2 cut(s) 380, 529
Bse8I GATNNNNATC 2 cut(s) 207, 774
BseCI ATCGAT 1 cut(s) 518
BseGI GGATG 5 cut(s) 83, 368, 484, 790, 812
BseJI GATNNNNATC 2 cut(s) 207, 774
BseLI CCNNNNNNNGG 2 cut(s) 190, 418
BseMII CTCAG 2 cut(s) 636, 639
BsePI GCGCGC 1 cut(s) 174
BseXI GCAGC 1 cut(s) 479
Bsh1236I CGCG 2 cut(s) 176, 282
BshFI GGCC 2 cut(s) 459, 811
BshVI ATCGAT 1 cut(s) 518
BsiHKAI GWGCWC 1 cut(s) 869
BsiSI CCGG 5 cut(s) 143, 184, 381, 412, 530
BslI CCNNNNNNNGG 2 cut(s) 190, 418
BsmAI GTCTC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 711
BsnI GGCC 2 cut(s) 459, 811
Bsp1286I GDGCHC 2 cut(s) 131, 869
Bsp143I GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
BspACI CCGC 4 cut(s) 338, 375, 378, 886
BspANI GGCC 2 cut(s) 459, 811
BspCNI CTCAG 2 cut(s) 635, 640
BspDI ATCGAT 1 cut(s) 518
BspFNI CGCG 2 cut(s) 176, 282
BspHI TCATGA 2 cut(s) 343, 691
BspLI GGNNCC 1 cut(s) 855
BspPI GGATC 3 cut(s) 564, 848, 861
BsrFI RCCGGY 2 cut(s) 380, 529
BssAI RCCGGY 2 cut(s) 380, 529
BssHII GCGCGC 1 cut(s) 174
BssMI GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
BssNI GRCGYC 1 cut(s) 627
Bst4CI ACNGT 6 cut(s) 164, 221, 409, 606, 634, 686
Bst6I CTCTTC 1 cut(s) 470
BstACI GRCGYC 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 176, 382, 937
BstDEI CTNAG 3 cut(s) 288, 622, 648
BstF5I GGATG 5 cut(s) 83, 368, 484, 790, 812
BstFNI CGCG 2 cut(s) 176, 282
BstH2I RGCGCY 1 cut(s) 803
BstHHI GCGC 4 cut(s) 176, 178, 618, 802
BstKTI GATC 8 cut(s) 6, 99, 211, 540, 548, 572, 778, 856
BstMAI GTCTC 1 cut(s) 595
BstMBI GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
BstMWI GCNNNNNNNGC 5 cut(s) 57, 180, 744, 808, 936
BstPAI GACNNNNGTC 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 411
BstSFI CTRYAG 1 cut(s) 163
BstUI CGCG 2 cut(s) 176, 282
BstV1I GCAGC 1 cut(s) 479
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
Bsu15I ATCGAT 1 cut(s) 518
BsuRI GGCC 2 cut(s) 459, 811
BsuTUI ATCGAT 1 cut(s) 518
BtrI CACGTC 1 cut(s) 246
BtsCI GGATG 5 cut(s) 83, 368, 484, 790, 812
BtsIMutI CAGTG 3 cut(s) 106, 226, 548
Cac8I GCNNGC 3 cut(s) 176, 382, 937
CciI TCATGA 2 cut(s) 343, 691
CfoI GCGC 4 cut(s) 176, 178, 618, 802
Cfr10I RCCGGY 2 cut(s) 380, 529
Cfr13I GGNCC 3 cut(s) 362, 409, 458
ClaI ATCGAT 1 cut(s) 518
CpoI CGGWCCG 1 cut(s) 409
CseI GACGC 1 cut(s) 635
Csp6I GTAC 2 cut(s) 92, 217
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
CspI CGGWCCG 1 cut(s) 409
CviAII CATG 4 cut(s) 7, 344, 692, 881
CviQI GTAC 2 cut(s) 92, 217
DdeI CTNAG 3 cut(s) 288, 622, 648
DpnI GATC 8 cut(s) 5, 98, 210, 539, 547, 571, 777, 855
DpnII GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
DraI TTTAAA 1 cut(s) 681
EaeI YGGCCR 1 cut(s) 809
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
EciI GGCGGA 1 cut(s) 364
Ecl136II GAGCTC 1 cut(s) 867
Eco24I GRGCYC 2 cut(s) 131, 869
Eco47I GGWCC 2 cut(s) 362, 409
Eco47III AGCGCT 1 cut(s) 801
Eco53kI GAGCTC 1 cut(s) 867
EcoICRI GAGCTC 1 cut(s) 867
EcoT22I ATGCAT 1 cut(s) 882
EcoT38I GRGCYC 2 cut(s) 131, 869
FaeI CATG 4 cut(s) 10, 347, 695, 884
FaiI YATR 6 cut(s) 8, 320, 345, 693, 797, 882
FatI CATG 4 cut(s) 6, 343, 691, 880
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 378, 493
FokI GGATG 5 cut(s) 70, 355, 471, 797, 819
FriOI GRGCYC 2 cut(s) 131, 869
Fsp4HI GCNGC 2 cut(s) 378, 493
FspBI CTAG 2 cut(s) 311, 958
GlaI GCGC 4 cut(s) 175, 177, 617, 801
GluI GCNGC 2 cut(s) 378, 493
HaeII RGCGCY 1 cut(s) 803
HaeIII GGCC 2 cut(s) 459, 811
HapII CCGG 5 cut(s) 143, 184, 381, 412, 530
HgaI GACGC 1 cut(s) 635
HhaI GCGC 4 cut(s) 176, 178, 618, 802
Hin1I GRCGYC 1 cut(s) 627
Hin1II CATG 4 cut(s) 10, 347, 695, 884
Hin6I GCGC 4 cut(s) 174, 176, 616, 800
HinP1I GCGC 4 cut(s) 174, 176, 616, 800
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HinfI GANTC 3 cut(s) 79, 580, 821
HpaII CCGG 5 cut(s) 143, 184, 381, 412, 530
HphI GGTGA 6 cut(s) 22, 236, 326, 327, 427, 709
Hpy166II GTNNAC 1 cut(s) 432
Hpy188I TCNGA 7 cut(s) 202, 250, 545, 585, 625, 784, 871
Hpy188III TCNNGA 5 cut(s) 115, 344, 499, 611, 692
Hpy8I GTNNAC 1 cut(s) 432
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 6 cut(s) 164, 221, 409, 606, 634, 686
HpyCH4IV ACGT 2 cut(s) 215, 245
HpyCH4V TGCA 3 cut(s) 657, 719, 880
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 180, 744, 808, 936
HpyF3I CTNAG 3 cut(s) 288, 622, 648
HpySE526I ACGT 2 cut(s) 215, 245
Hsp92I GRCGYC 1 cut(s) 627
Hsp92II CATG 4 cut(s) 10, 347, 695, 884
HspAI GCGC 4 cut(s) 174, 176, 616, 800
KroI GCCGGC 1 cut(s) 380
KroNI GCCGGC 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
LmnI GCTCC 2 cut(s) 193, 864
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 5 cut(s) 513, 524, 627, 666, 867
MaeI CTAG 2 cut(s) 311, 958
MaeII ACGT 2 cut(s) 215, 245
MaeIII GTNAC 5 cut(s) 167, 224, 283, 314, 728
MalI GATC 8 cut(s) 5, 98, 210, 539, 547, 571, 777, 855
MboI GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
MboII GAAGA 6 cut(s) 31, 126, 457, 460, 514, 547
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 2 cut(s) 131, 869
MluCI AATT 5 cut(s) 331, 596, 652, 872, 914
MlyI GAGTC 1 cut(s) 830
Mph1103I ATGCAT 1 cut(s) 882
MroNI GCCGGC 1 cut(s) 380
MseI TTAA 2 cut(s) 36, 680
MslI CAYNNNNRTG 1 cut(s) 348
MspI CCGG 5 cut(s) 143, 184, 381, 412, 530
MspR9I CCNGG 1 cut(s) 413
Mva1269I GAATGC 1 cut(s) 711
MvnI CGCG 2 cut(s) 176, 282
MwoI GCNNNNNNNGC 5 cut(s) 57, 180, 744, 808, 936
NaeI GCCGGC 1 cut(s) 382
NciI CCSGG 1 cut(s) 413
NdeII GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
NgoMIV GCCGGC 1 cut(s) 380
NlaIII CATG 4 cut(s) 10, 347, 695, 884
NlaIV GGNNCC 1 cut(s) 855
NmeAIII GCCGAG 1 cut(s) 435
NmuCI GTSAC 5 cut(s) 167, 224, 283, 314, 728
NsiI ATGCAT 1 cut(s) 882
PagI TCATGA 2 cut(s) 343, 691
PauI GCGCGC 1 cut(s) 174
PctI GAATGC 1 cut(s) 711
PdiI GCCGGC 1 cut(s) 382
PfeI GAWTC 2 cut(s) 79, 580
PflFI GACNNNGTC 1 cut(s) 606
PkrI GCNGC 2 cut(s) 379, 494
PleI GAGTC 1 cut(s) 829
PpsI GAGTC 1 cut(s) 829
Ppu21I YACGTR 1 cut(s) 216
PshAI GACNNNNGTC 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 869
PspN4I GGNNCC 1 cut(s) 855
PspPI GGNCC 3 cut(s) 362, 409, 458
PsuI RGATCY 1 cut(s) 853
PsyI GACNNNGTC 1 cut(s) 606
PteI GCGCGC 1 cut(s) 174
RsaI GTAC 2 cut(s) 93, 218
RsaNI GTAC 2 cut(s) 92, 217
RseI CAYNNNNRTG 1 cut(s) 348
Rsr2I CGGWCCG 1 cut(s) 409
RsrII CGGWCCG 1 cut(s) 409
SacI GAGCTC 1 cut(s) 869
SaqAI TTAA 2 cut(s) 36, 680
SatI GCNGC 2 cut(s) 378, 493
Sau3AI GATC 8 cut(s) 3, 96, 208, 537, 545, 569, 775, 853
Sau96I GGNCC 3 cut(s) 362, 409, 458
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 2 cut(s) 131, 869
SfaNI GCATC 5 cut(s) 513, 524, 627, 666, 867
SfcI CTRYAG 1 cut(s) 163
SgrAI CRCCGGYG 1 cut(s) 529
SinI GGWCC 2 cut(s) 362, 409
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 2 cut(s) 611, 844
SmoI CTYRAG 2 cut(s) 611, 844
Sse9I AATT 5 cut(s) 331, 596, 652, 872, 914
SsiI CCGC 4 cut(s) 338, 375, 378, 886
SspMI CTAG 2 cut(s) 311, 958
SstI GAGCTC 1 cut(s) 869
StyD4I CCNGG 1 cut(s) 411
TaaI ACNGT 6 cut(s) 164, 221, 409, 606, 634, 686
TaiI ACGT 2 cut(s) 218, 248
TaqI TCGA 2 cut(s) 423, 518
TasI AATT 5 cut(s) 331, 596, 652, 872, 914
TatI WGTACW 1 cut(s) 91
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 2 cut(s) 79, 580
Tru1I TTAA 2 cut(s) 36, 680
Tru9I TTAA 2 cut(s) 36, 680
TscAI CASTG 3 cut(s) 106, 226, 555
TseFI GTSAC 5 cut(s) 167, 224, 283, 314, 728
TseI GCWGC 1 cut(s) 492
Tsp45I GTSAC 5 cut(s) 167, 224, 283, 314, 728
TspDTI ATGAA 2 cut(s) 126, 907
TspRI CASTG 3 cut(s) 106, 226, 555
Tth111I GACNNNGTC 1 cut(s) 606
VpaK11BI GGWCC 2 cut(s) 362, 409
XspI CTAG 2 cut(s) 311, 958
Zsp2I ATGCAT 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.