FvH4_6g45550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000735
Physical Location & Seq
Reverse (-)
34949114 .. 34949500
387 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45550.t1

Sequence Viewer

Length: 387 bp
ATGGCCATCGACACTTTCTGCGACATACTTGTCACCTTCATTGAACCAGAAAAGGAGTTTGTATGCACCACGAATAAGTTCTTGATGCAATTCGAGGCGGTGACCAGACGTGTCAGTTCCGACCAGGTGATCACGAAGAGGACCTCGATCGAACTTGACGACTCTAACGACCCAGCATACATGGATGAAGCCCTATCCGAAGCGCTTCTACAGCTGCTAGTCCCACGGCAGGAGCGTAGTTCGTTGGTGAATAAAGTAATGTTGAAAGCCTCCGAGGTGGCGCAGAAACCTAGTCCTTATGCCGGTGGGAAGAGACTGCGGATGCGAGTGAACATCGATATGTTTGTGATTGATGATCATTATGAGAAACTGGAGAGGACGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

14.73

Weight (kDa)

5.06

Isoelectric Point (pI)

69.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 29
AciI CCGC 2 cut(s) 98, 319
AcoI YGGCCR 1 cut(s) 3
AfeI AGCGCT 1 cut(s) 204
AfiI CCNNNNNNNGG 2 cut(s) 229, 302
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 2 cut(s) 44, 265
AjiI CACGTC 1 cut(s) 110
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 307
Aor51HI AGCGCT 1 cut(s) 204
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 214
Asp700I GAANNNNTTC 2 cut(s) 77, 204
AspLEI GCGC 2 cut(s) 205, 283
AspS9I GGNCC 1 cut(s) 141
AsuHPI GGTGA 4 cut(s) 25, 112, 139, 259
AvaII GGWCC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 192, 224
BbvI GCAGC 1 cut(s) 201
BccI CCATC 1 cut(s) 14
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 2 cut(s) 129, 355
BcoDI GTCTC 1 cut(s) 307
BfaI CTAG 2 cut(s) 218, 291
BfmI CTRYAG 1 cut(s) 209
BfoI RGCGCY 1 cut(s) 206
BisI GCNGC 1 cut(s) 215
BlsI GCNGC 1 cut(s) 216
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 141
BmgBI CACGTC 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 141
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 75, 312
Bsa29I ATCGAT 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 224, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 302
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 375
BseBI CCWGG 1 cut(s) 125
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 224, 273
BseGI GGATG 2 cut(s) 190, 327
BseLI CCNNNNNNNGG 2 cut(s) 229, 302
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 1 cut(s) 201
BseYI CCCAGC 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 303
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 229, 302
BsmAI GTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 3 cut(s) 129, 147, 355
BspACI CCGC 2 cut(s) 98, 319
BspANI GGCC 1 cut(s) 5
BspDI ATCGAT 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 1 cut(s) 375
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 2 cut(s) 224, 273
BssMI GATC 3 cut(s) 129, 147, 355
Bst2UI CCWGG 1 cut(s) 125
Bst6I CTCTTC 2 cut(s) 131, 305
BstDSI CCRYGG 1 cut(s) 224
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 2 cut(s) 190, 327
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 2 cut(s) 205, 283
BstKTI GATC 3 cut(s) 132, 150, 358
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 3 cut(s) 129, 147, 355
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 1 cut(s) 211
BstNI CCWGG 1 cut(s) 125
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 209
BstV1I GCAGC 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 336
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 224
BtrI CACGTC 1 cut(s) 110
BtsCI GGATG 2 cut(s) 190, 327
CfoI GCGC 2 cut(s) 205, 283
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 141
ClaI ATCGAT 1 cut(s) 336
CsiI ACCWGGT 1 cut(s) 123
CviAII CATG 1 cut(s) 181
CviJI RGCY 4 cut(s) 5, 191, 214, 269
CviKI_1 RGCY 4 cut(s) 5, 191, 214, 269
DpnI GATC 3 cut(s) 131, 149, 357
DpnII GATC 3 cut(s) 129, 147, 355
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 131, 305
EarI CTCTTC 2 cut(s) 131, 305
Eco47I GGWCC 1 cut(s) 141
Eco47III AGCGCT 1 cut(s) 204
Eco91I GGTNACC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 141
EcoO65I GGTNACC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 123
FaeI CATG 1 cut(s) 184
FaiI YATR 8 cut(s) 26, 64, 178, 182, 300, 341, 363, 385
FaqI GGGAC 1 cut(s) 206
FatI CATG 1 cut(s) 180
FbaI TGATCA 2 cut(s) 129, 355
Fnu4HI GCNGC 1 cut(s) 215
FokI GGATG 2 cut(s) 197, 334
Fsp4HI GCNGC 1 cut(s) 215
FspBI CTAG 2 cut(s) 218, 291
GlaI GCGC 2 cut(s) 204, 282
GluI GCNGC 1 cut(s) 215
GsaI CCCAGC 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 303
HhaI GCGC 2 cut(s) 205, 283
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 2 cut(s) 203, 281
HinP1I GCGC 2 cut(s) 203, 281
HinfI GANTC 1 cut(s) 161
HpaII CCGG 1 cut(s) 303
HphI GGTGA 4 cut(s) 25, 112, 139, 259
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 121, 199, 274
Hpy188III TCNNGA 2 cut(s) 82, 133
Hpy8I GTNNAC 1 cut(s) 331
HpyAV CCTTC 1 cut(s) 46
HpyCH4IV ACGT 1 cut(s) 109
HpyCH4V TGCA 2 cut(s) 66, 88
HpyF10VI GCNNNNNNNGC 1 cut(s) 211
HpySE526I ACGT 1 cut(s) 109
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 2 cut(s) 203, 281
Ksp22I TGATCA 2 cut(s) 129, 355
Kzo9I GATC 3 cut(s) 129, 147, 355
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 8 cut(s) 60, 110, 118, 137, 186, 215, 316, 356
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 2 cut(s) 75, 312
MabI ACCWGGT 1 cut(s) 123
MaeI CTAG 2 cut(s) 218, 291
MaeII ACGT 1 cut(s) 109
MaeIII GTNAC 2 cut(s) 31, 100
MalI GATC 3 cut(s) 131, 149, 357
MboI GATC 3 cut(s) 129, 147, 355
MboII GAAGA 2 cut(s) 148, 322
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 89
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 6 cut(s) 88, 132, 154, 268, 280, 369
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 77, 204
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 211
NdeII GATC 3 cut(s) 129, 147, 355
NlaIII CATG 1 cut(s) 184
NmuCI GTSAC 2 cut(s) 31, 100
PcsI WCGNNNNNNNCGW 3 cut(s) 156, 165, 232
PdmI GAANNNNTTC 2 cut(s) 77, 204
PkrI GCNGC 1 cut(s) 216
Ple19I CGATCG 1 cut(s) 150
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 141
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 123
PspEI GGTNACC 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 1 cut(s) 123
PspPI GGNCC 1 cut(s) 141
PspPPI RGGWCCY 1 cut(s) 141
PvuI CGATCG 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 338
SatI GCNGC 1 cut(s) 215
Sau3AI GATC 3 cut(s) 129, 147, 355
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 125
SetI ASST 7 cut(s) 38, 112, 129, 146, 216, 279, 292
SexAI ACCWGGT 1 cut(s) 123
SfaNI GCATC 2 cut(s) 75, 312
SfcI CTRYAG 1 cut(s) 209
SinI GGWCC 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 1 cut(s) 89
SsiI CCGC 2 cut(s) 98, 319
SspMI CTAG 2 cut(s) 218, 291
StyD4I CCNGG 1 cut(s) 123
TaiI ACGT 1 cut(s) 112
TaqI TCGA 5 cut(s) 9, 93, 146, 150, 336
TasI AATT 1 cut(s) 89
TseFI GTSAC 2 cut(s) 31, 100
TseI GCWGC 1 cut(s) 214
Tsp45I GTSAC 2 cut(s) 31, 100
TspDTI ATGAA 2 cut(s) 28, 201
VpaK11BI GGWCC 1 cut(s) 141
XmnI GAANNNNTTC 2 cut(s) 77, 204
XspI CTAG 2 cut(s) 218, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.