Rroxscaffold_1G00022030

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000735
Physical Location & Seq
Forward (+)
27136987 .. 27137463
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022030.1

Sequence Viewer

Length: 477 bp
ATGTCCATGGCTACTGAAAATTGGCAAGTGGTGGGTGACATCACCTGCAACCCAGGATACCTAGGTCGTGTTCAACCTGGAAAGGAGCTGCCTTACACCAAATTCTTGATGGATTTCAATATCAAGATGAGATGCGCGCAACCTATTGATTTGGAGGGTGATGAAGAAATTGTAGAAGAGAAGAATCTCCACTCAGTCGAACTTGAGTACAACCCAAATAGCATGTGGGTTGCCATAAATAAAGAGCTTCAAGACCTGCGAGTTCCGAGCCAAGTTCGTGCAGCCATTGAAGATCCAATATTACTCAAGGGAACCAGAGTGGCACGAGCTGATAACTCTTATGACGCTCACAAGTATCTACATATGGGGGTGGATATTGATTTTCGTGTTGGCGGTTTTGATGGTATTGGTGAATATGAGCCTATGTTTGTGCCGGCAAGTGAAGCGTTCATTAAAAAACTAGAGAAGACGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

158

Amino Acids

17.93

Weight (kDa)

4.81

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 53
Acc36I ACCTGC 2 cut(s) 53, 264
AccII CGCG 1 cut(s) 137
AciI CCGC 1 cut(s) 393
AclWI GGATC 1 cut(s) 287
AcsI RAATTY 1 cut(s) 101
AfaI GTAC 1 cut(s) 209
AgsI TTSAA 4 cut(s) 74, 118, 251, 290
AjnI CCWGG 2 cut(s) 52, 76
AluBI AGCT 4 cut(s) 88, 247, 329, 474
AluI AGCT 4 cut(s) 88, 247, 329, 474
AlwI GGATC 1 cut(s) 287
ApeKI GCWGC 2 cut(s) 88, 281
ApoI RAATTY 1 cut(s) 101
AspA2I CCTAGG 1 cut(s) 61
AspLEI GCGC 2 cut(s) 137, 139
AsuHPI GGTGA 4 cut(s) 34, 47, 170, 422
AvrII CCTAGG 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 49, 82
BauI CACGAG 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 473
BbvI GCAGC 2 cut(s) 75, 293
BccI CCATC 2 cut(s) 103, 395
BciT130I CCWGG 2 cut(s) 54, 78
BciVI GTATCC 1 cut(s) 50
BfaI CTAG 2 cut(s) 62, 461
BfuAI ACCTGC 2 cut(s) 53, 264
BfuI GTATCC 1 cut(s) 50
BisI GCNGC 2 cut(s) 89, 282
BlnI CCTAGG 1 cut(s) 61
BlsI GCNGC 2 cut(s) 90, 283
Bme1390I CCNGG 2 cut(s) 54, 78
BmiI GGNNCC 1 cut(s) 313
BmrFI CCNGG 2 cut(s) 54, 78
BmsI GCATC 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 473
BpuEI CTTGAG 2 cut(s) 224, 290
BsaJI CCNNGG 3 cut(s) 6, 52, 61
Bse118I RCCGGY 1 cut(s) 433
BseBI CCWGG 2 cut(s) 54, 78
BseDI CCNNGG 3 cut(s) 6, 52, 61
BseMII CTCAG 1 cut(s) 207
BsePI GCGCGC 1 cut(s) 135
BseXI GCAGC 2 cut(s) 75, 293
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 434
Bsp143I GATC 1 cut(s) 292
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 1 cut(s) 393
BspCNI CTCAG 1 cut(s) 206
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 313
BspMI ACCTGC 2 cut(s) 53, 264
BspPI GGATC 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 433
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 3 cut(s) 6, 52, 61
BssHII GCGCGC 1 cut(s) 135
BssMI GATC 1 cut(s) 292
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 6, 61
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 54, 78
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 137, 435
BstDEI CTNAG 1 cut(s) 193
BstDSI CCRYGG 1 cut(s) 6
BstFNI CGCG 1 cut(s) 137
BstHHI GCGC 2 cut(s) 137, 139
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 2 cut(s) 54, 78
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 52, 76
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 2 cut(s) 75, 293
BstV2I GAAGAC 1 cut(s) 473
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuI GTATCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 6
BveI ACCTGC 2 cut(s) 53, 264
Cac8I GCNNGC 2 cut(s) 137, 435
CfoI GCGC 2 cut(s) 137, 139
Cfr10I RCCGGY 1 cut(s) 433
CseI GACGC 1 cut(s) 353
Csp6I GTAC 1 cut(s) 208
CviAII CATG 2 cut(s) 7, 223
CviJI RGCY 8 cut(s) 11, 88, 247, 270, 284, 329, 421, 474
CviKI_1 RGCY 8 cut(s) 11, 88, 247, 270, 284, 329, 421, 474
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 1 cut(s) 193
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 6, 61
EcoRII CCWGG 2 cut(s) 52, 76
EcoT14I CCWWGG 2 cut(s) 6, 61
ErhI CCWWGG 2 cut(s) 6, 61
FaeI CATG 2 cut(s) 10, 226
FaiI YATR 8 cut(s) 8, 224, 236, 342, 363, 365, 417, 425
FatI CATG 2 cut(s) 6, 222
FauNDI CATATG 1 cut(s) 363
Fnu4HI GCNGC 2 cut(s) 89, 282
Fsp4HI GCNGC 2 cut(s) 89, 282
FspBI CTAG 2 cut(s) 62, 461
GlaI GCGC 2 cut(s) 136, 138
GluI GCNGC 2 cut(s) 89, 282
HapII CCGG 1 cut(s) 434
HgaI GACGC 1 cut(s) 353
HhaI GCGC 2 cut(s) 137, 139
Hin1II CATG 2 cut(s) 10, 226
Hin6I GCGC 2 cut(s) 135, 137
HinP1I GCGC 2 cut(s) 135, 137
HinfI GANTC 1 cut(s) 184
HpaII CCGG 1 cut(s) 434
HphI GGTGA 4 cut(s) 34, 47, 170, 422
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 3 cut(s) 106, 124, 251
HpyCH4V TGCA 2 cut(s) 48, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 193
Hsp92II CATG 2 cut(s) 10, 226
HspAI GCGC 2 cut(s) 135, 137
KroI GCCGGC 1 cut(s) 433
KroNI GCCGGC 1 cut(s) 435
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 85
LpnPI CCDG 8 cut(s) 39, 58, 63, 66, 90, 269, 328, 447
Lsp1109I GCAGC 2 cut(s) 75, 293
LweI GCATC 1 cut(s) 122
MaeI CTAG 2 cut(s) 62, 461
MaeIII GTNAC 1 cut(s) 35
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 4 cut(s) 176, 188, 193, 302
MflI RGATCY 1 cut(s) 292
MluCI AATT 3 cut(s) 19, 101, 168
MnlI CCTC 1 cut(s) 148
MroNI GCCGGC 1 cut(s) 433
MseI TTAA 1 cut(s) 453
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 2 cut(s) 54, 78
MvaI CCWGG 2 cut(s) 54, 78
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 443
NaeI GCCGGC 1 cut(s) 435
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 363
NdeII GATC 1 cut(s) 292
NgoMIV GCCGGC 1 cut(s) 433
NlaIII CATG 2 cut(s) 10, 226
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 53
PauI GCGCGC 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 435
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 2 cut(s) 90, 283
Psp6I CCWGG 2 cut(s) 52, 76
PspGI CCWGG 2 cut(s) 52, 76
PspN4I GGNNCC 1 cut(s) 313
PsuI RGATCY 1 cut(s) 292
PteI GCGCGC 1 cut(s) 135
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 89, 282
Sau3AI GATC 1 cut(s) 292
ScrFI CCNGG 2 cut(s) 54, 78
SfaNI GCATC 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 203, 305
SmoI CTYRAG 2 cut(s) 203, 305
Sse9I AATT 3 cut(s) 19, 101, 168
SsiI CCGC 1 cut(s) 393
SspI AATATT 1 cut(s) 300
SspMI CTAG 2 cut(s) 62, 461
StyD4I CCNGG 2 cut(s) 52, 76
StyI CCWWGG 2 cut(s) 6, 61
TaqI TCGA 1 cut(s) 198
TasI AATT 3 cut(s) 19, 101, 168
TatI WGTACW 1 cut(s) 207
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 2 cut(s) 88, 281
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 2 cut(s) 177, 439
XapI RAATTY 1 cut(s) 101
XceI RCATGY 1 cut(s) 226
XcmI CCANNNNNNNNNTGG 2 cut(s) 106, 222
XmaJI CCTAGG 1 cut(s) 61
XspI CTAG 2 cut(s) 62, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.