Rroxscaffold_1G00004750

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000735
Physical Location & Seq
Forward (+)
6511709 .. 6514754
3046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00004750.1

Sequence Viewer

Length: 564 bp
ATGAGGGGCAGTAAAGTTGGCAAAGAAAGCGTCGAGTTCATGACAAAGGCAATTCAGATTGTGGCATTCAAGCTTTATGGCCTATTTAGAGGCCTATATGGAATCAGTCAAGTAATTTCAGTCAAGCCAATACGAGTGGCTTTGGAGCAACGACATTATAAGAGCAGTGTTGATTCAAGACCTCTTCGGTCAAGACGAAGGCGTACCTTTGTCTTCGAGGTCGGAGGGGCAATGTTTGAACCCAAAATCTCTGAGCATTTTGACCTCGACAAGGCGTGTCTTGGAGAAATTGAGCCAATGAATCAGATTTTGATGTTGGAGGTGGAAGACATGATGAGTGAGGTTAAATTTGAGCCGGCGAGCAAAATGTCCATTGAGAAATTAGAGAGGGTGAGAATAGTGGAAGCATCAACCATGCGTGCTATATGTGCAGAGGAGATTGTGGTTGGTTCCGAAGAAATTCAGATGCTATGTTCGCATTTTTACCATATCGATTGCATTGTCAAGTTGTTGGAGCAGAGCATGTTTTGCCACAATGTCAATATGCTGTGCTGCAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

187

Amino Acids

21.5

Weight (kDa)

6.95

Isoelectric Point (pI)

61.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 159
AcsI RAATTY 2 cut(s) 347, 459
AfaI GTAC 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 3 cut(s) 70, 177, 239
AluBI AGCT 2 cut(s) 73, 558
AluI AGCT 2 cut(s) 73, 558
AoxI GGCC 2 cut(s) 79, 91
ApeKI GCWGC 2 cut(s) 552, 555
ApoI RAATTY 2 cut(s) 347, 459
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
Asp700I GAANNNNTTC 1 cut(s) 459
AsuHPI GGTGA 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 205, 333
BbvI GCAGC 1 cut(s) 539
BfmI CTRYAG 1 cut(s) 553
BisI GCNGC 2 cut(s) 553, 556
BlsI GCNGC 2 cut(s) 554, 557
BmiI GGNNCC 1 cut(s) 451
BmsI GCATC 2 cut(s) 416, 456
BpiI GAAGAC 2 cut(s) 205, 333
Bsa29I ATCGAT 1 cut(s) 492
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse118I RCCGGY 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 237
BseCI ATCGAT 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 1 cut(s) 243
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 2 cut(s) 81, 93
BshVI ATCGAT 1 cut(s) 492
BsiSI CCGG 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 271
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 2 cut(s) 81, 93
BspANI GGCC 2 cut(s) 81, 93
BspCNI CTCAG 1 cut(s) 244
BspDI ATCGAT 1 cut(s) 492
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 451
BspMAI CTGCAG 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 355
BssAI RCCGGY 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 528
BstC8I GCNNGC 3 cut(s) 357, 361, 420
BstDEI CTNAG 1 cut(s) 252
BstENI CCTNNNNNAGG 1 cut(s) 269
BstMWI GCNNNNNNNGC 4 cut(s) 27, 428, 475, 528
BstNSI RCATGY 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 539
BstV2I GAAGAC 2 cut(s) 205, 333
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 2 cut(s) 81, 93
BsuTUI ATCGAT 1 cut(s) 492
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 172
Cac8I GCNNGC 3 cut(s) 357, 361, 420
CciI TCATGA 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 355
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 1 cut(s) 19
Csp6I GTAC 1 cut(s) 204
CspCI CAANNNNNGTGG 2 cut(s) 117, 152
CviAII CATG 4 cut(s) 40, 331, 415, 523
CviJI RGCY 8 cut(s) 73, 81, 93, 127, 140, 295, 355, 558
CviKI_1 RGCY 8 cut(s) 73, 81, 93, 127, 140, 295, 355, 558
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 93
EcoNI CCTNNNNNAGG 1 cut(s) 269
FaeI CATG 4 cut(s) 43, 334, 418, 526
FatI CATG 4 cut(s) 39, 330, 414, 522
Fnu4HI GCNGC 2 cut(s) 553, 556
Fsp4HI GCNGC 2 cut(s) 553, 556
GluI GCNGC 2 cut(s) 553, 556
HaeIII GGCC 2 cut(s) 81, 93
HapII CCGG 1 cut(s) 356
HgaI GACGC 1 cut(s) 19
Hin1II CATG 4 cut(s) 43, 334, 418, 526
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 102, 173, 301
HpaII CCGG 1 cut(s) 356
HphI GGTGA 1 cut(s) 403
Hpy188I TCNGA 6 cut(s) 57, 224, 253, 306, 454, 465
Hpy188III TCNNGA 3 cut(s) 40, 177, 192
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 1 cut(s) 192
HpyCH4V TGCA 3 cut(s) 431, 498, 555
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 428, 475, 528
HpyF3I CTNAG 1 cut(s) 252
Hsp92II CATG 4 cut(s) 43, 334, 418, 526
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
LmnI GCTCC 2 cut(s) 145, 514
LpnPI CCDG 1 cut(s) 369
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 2 cut(s) 416, 456
MboII GAAGA 4 cut(s) 176, 205, 338, 467
MluCI AATT 6 cut(s) 51, 114, 288, 347, 380, 459
MmeI TCCRAC 3 cut(s) 202, 297, 492
MnlI CCTC 9 cut(s) 83, 192, 211, 218, 275, 313, 334, 381, 427
MroNI GCCGGC 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 459
MseI TTAA 1 cut(s) 345
MspI CCGG 1 cut(s) 356
Mva1269I GAATGC 1 cut(s) 65
MwoI GCNNNNNNNGC 4 cut(s) 27, 428, 475, 528
NaeI GCCGGC 1 cut(s) 357
NgoMIV GCCGGC 1 cut(s) 355
NlaIII CATG 4 cut(s) 43, 334, 418, 526
NlaIV GGNNCC 1 cut(s) 451
NspI RCATGY 1 cut(s) 526
PagI TCATGA 1 cut(s) 39
PceI AGGCCT 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PctI GAATGC 1 cut(s) 65
PdiI GCCGGC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 3 cut(s) 102, 173, 301
PkrI GCNGC 2 cut(s) 554, 557
PsiI TTATAA 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 451
PstI CTGCAG 1 cut(s) 557
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 2 cut(s) 553, 556
SetI ASST 8 cut(s) 75, 184, 209, 222, 267, 324, 345, 560
SfaNI GCATC 2 cut(s) 416, 456
SfcI CTRYAG 1 cut(s) 553
Sse9I AATT 6 cut(s) 51, 114, 288, 347, 380, 459
SseBI AGGCCT 1 cut(s) 93
StuI AGGCCT 1 cut(s) 93
TaqI TCGA 4 cut(s) 33, 216, 267, 492
TaqII GACCGA 1 cut(s) 177
TasI AATT 6 cut(s) 51, 114, 288, 347, 380, 459
TfiI GAWTC 3 cut(s) 102, 173, 301
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 1 cut(s) 172
TseI GCWGC 2 cut(s) 552, 555
TspDTI ATGAA 2 cut(s) 28, 314
TspRI CASTG 1 cut(s) 172
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 2 cut(s) 347, 459
XceI RCATGY 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.