Rroxscaffold_1G00022050

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000735
Physical Location & Seq
Forward (+)
27145636 .. 27146951
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022050.1

Sequence Viewer

Length: 618 bp
ATGGCTACAGAAAATTGGCAAGTGGTGGGTGACATCACCTGCAACCCAAAATACCTAGGTCTTGTTCAACCTGAAAAGGAGCTGTCTAACACCAAATTCTTGATGGATTTCAATGTCAAGATGAGGCGCGCGCAGTCTACTGTATTGGAGGATGATGAAGAAATTGTAGAAGAGAAGAATCTGCGGTCAGTCGAACTTGAGTACAATCCACATAGCATGTGGGTTGCCATAAATAAAGAGCTTCAAGACCTGCAAGTTCCAATCCAAGTTCGTGCAGCCATTGAAGATCCAATACTCATGGGAACCAGAGTCGCACGAGCCGATAGCTCTTATGACGCTCGCAAGTATCTGCATATGGAGGTGGCTATCGATTTTCGTGTTGGCGTTTTTGATGGTATTAGTGAATATGAGCCTACGTTTGTGCCGGCAAGTGAAGCGTCCATTAAGAAACTGGGGAAGACAAGAGCCGAAGTGTCGTCAACAATGTGCACGAGGGCAATGGCCATGGCCAGTGAACACTTGAAAGTACTAGGCAACCCTTACTGTGATGTACACTTTCTTGGTGAAAGAGAAGTTGCCATCGACAAAATTTTTGATGGATTTCGAGGCCATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_3 zf-RING_2

Protein Analysis

205

Amino Acids

23.28

Weight (kDa)

5.05

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 2 cut(s) 47, 258
AccI GTMKAC 1 cut(s) 137
AccII CGCG 2 cut(s) 129, 131
AciI CCGC 1 cut(s) 184
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 2 cut(s) 501, 507
AcsI RAATTY 2 cut(s) 95, 588
AfaI GTAC 3 cut(s) 203, 528, 552
AgsI TTSAA 5 cut(s) 68, 112, 245, 284, 523
AluBI AGCT 3 cut(s) 82, 241, 327
AluI AGCT 3 cut(s) 82, 241, 327
Alw21I GWGCWC 1 cut(s) 491
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 1 cut(s) 281
AoxI GGCC 3 cut(s) 501, 507, 607
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 2 cut(s) 95, 588
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AspA2I CCTAGG 1 cut(s) 55
AspLEI GCGC 3 cut(s) 129, 131, 133
AsuHPI GGTGA 3 cut(s) 28, 41, 575
AvrII CCTAGG 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 491
BalI TGGCCA 2 cut(s) 503, 509
BarI GAAGNNNNNNTAC 2 cut(s) 276, 308
BauI CACGAG 2 cut(s) 315, 490
BbsI GAAGAC 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 1 cut(s) 287
BccI CCATC 4 cut(s) 97, 386, 587, 590
BfaI CTAG 2 cut(s) 56, 530
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 2 cut(s) 47, 258
BisI GCNGC 1 cut(s) 276
BlnI CCTAGG 1 cut(s) 55
BlsI GCNGC 1 cut(s) 277
BmcAI AGTACT 1 cut(s) 528
BmiI GGNNCC 1 cut(s) 304
BmrI ACTGGG 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 461
BpiI GAAGAC 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 218
Bsa29I ATCGAT 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 55, 504
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 2 cut(s) 456, 510
Bse3DI GCAATG 1 cut(s) 504
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 55, 504
BseGI GGATG 1 cut(s) 157
BseMI GCAATG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 456, 510
BsePI GCGCGC 2 cut(s) 127, 129
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 1 cut(s) 287
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 2 cut(s) 129, 131
BshFI GGCC 3 cut(s) 503, 509, 609
BshVI ATCGAT 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 425
BsnI GGCC 3 cut(s) 503, 509, 609
Bsp1286I GDGCHC 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 550
Bsp143I GATC 1 cut(s) 286
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 1 cut(s) 184
BspANI GGCC 3 cut(s) 503, 509, 609
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 2 cut(s) 129, 131
BspLI GGNNCC 1 cut(s) 304
BspMI ACCTGC 2 cut(s) 47, 258
BspPI GGATC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 504
BsrFI RCCGGY 1 cut(s) 424
BsrGI TGTACA 1 cut(s) 550
BsrI ACTGG 2 cut(s) 456, 510
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 2 cut(s) 55, 504
BssHII GCGCGC 2 cut(s) 127, 129
BssMI GATC 1 cut(s) 286
BssSI CACGAG 2 cut(s) 315, 490
BssT1I CCWWGG 2 cut(s) 55, 504
Bst2BI CACGAG 2 cut(s) 315, 490
Bst4CI ACNGT 2 cut(s) 142, 545
Bst6I CTCTTC 1 cut(s) 165
BstAUI TGTACA 1 cut(s) 550
BstC8I GCNNGC 4 cut(s) 129, 131, 340, 426
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 2 cut(s) 129, 131
BstHHI GCGC 3 cut(s) 129, 131, 133
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 491
BstUI CGCG 2 cut(s) 129, 131
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 369
BsuRI GGCC 3 cut(s) 503, 509, 609
BsuTUI ATCGAT 1 cut(s) 369
BtgI CCRYGG 1 cut(s) 504
BtsCI GGATG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 517
BveI ACCTGC 2 cut(s) 47, 258
Cac8I GCNNGC 4 cut(s) 129, 131, 340, 426
CfoI GCGC 3 cut(s) 129, 131, 133
Cfr10I RCCGGY 1 cut(s) 424
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 2 cut(s) 344, 426
Csp6I GTAC 3 cut(s) 202, 527, 551
CviAII CATG 4 cut(s) 217, 298, 505, 611
CviQI GTAC 3 cut(s) 202, 527, 551
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
EaeI YGGCCR 2 cut(s) 501, 507
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 55, 504
EcoT14I CCWWGG 2 cut(s) 55, 504
ErhI CCWWGG 2 cut(s) 55, 504
FaeI CATG 4 cut(s) 220, 301, 508, 614
FatI CATG 4 cut(s) 216, 297, 504, 610
FauNDI CATATG 1 cut(s) 354
FblI GTMKAC 1 cut(s) 137
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 2 cut(s) 56, 530
GlaI GCGC 3 cut(s) 128, 130, 132
GluI GCNGC 1 cut(s) 276
HaeIII GGCC 3 cut(s) 503, 509, 609
HapII CCGG 1 cut(s) 425
HgaI GACGC 2 cut(s) 344, 426
HhaI GCGC 3 cut(s) 129, 131, 133
Hin1II CATG 4 cut(s) 220, 301, 508, 614
Hin6I GCGC 3 cut(s) 127, 129, 131
HinP1I GCGC 3 cut(s) 127, 129, 131
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 2 cut(s) 178, 309
HpaII CCGG 1 cut(s) 425
HphI GGTGA 3 cut(s) 28, 41, 575
Hpy166II GTNNAC 5 cut(s) 138, 480, 489, 515, 553
Hpy188III TCNNGA 3 cut(s) 100, 118, 245
Hpy8I GTNNAC 5 cut(s) 138, 480, 489, 515, 553
HpyCH4III ACNGT 2 cut(s) 142, 545
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 5 cut(s) 42, 253, 275, 352, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 4 cut(s) 220, 301, 508, 614
HspAI GCGC 3 cut(s) 127, 129, 131
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 7 cut(s) 52, 84, 263, 319, 437, 438, 523
Lsp1109I GCAGC 1 cut(s) 287
MaeI CTAG 2 cut(s) 56, 530
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 1 cut(s) 29
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 5 cut(s) 170, 182, 187, 296, 469
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 491
MlsI TGGCCA 2 cut(s) 503, 509
MluCI AATT 4 cut(s) 13, 95, 162, 588
MluNI TGGCCA 2 cut(s) 503, 509
MlyI GAGTC 1 cut(s) 318
MnlI CCTC 5 cut(s) 117, 142, 352, 486, 599
Mox20I TGGCCA 2 cut(s) 503, 509
MroNI GCCGGC 1 cut(s) 424
MscI TGGCCA 2 cut(s) 503, 509
MseI TTAA 2 cut(s) 444, 616
Msp20I TGGCCA 2 cut(s) 503, 509
MspI CCGG 1 cut(s) 425
MvnI CGCG 2 cut(s) 129, 131
MwoI GCNNNNNNNGC 1 cut(s) 434
NaeI GCCGGC 1 cut(s) 426
NcoI CCATGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 354
NdeII GATC 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 4 cut(s) 220, 301, 508, 614
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 47
PauI GCGCGC 2 cut(s) 127, 129
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 286
PteI GCGCGC 2 cut(s) 127, 129
RsaI GTAC 3 cut(s) 203, 528, 552
RsaNI GTAC 3 cut(s) 202, 527, 551
SaqAI TTAA 2 cut(s) 444, 616
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 1 cut(s) 286
ScaI AGTACT 1 cut(s) 528
SchI GAGTC 1 cut(s) 318
SduI GDGCHC 1 cut(s) 491
SfcI CTRYAG 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 4 cut(s) 13, 95, 162, 588
SsiI CCGC 1 cut(s) 184
SspMI CTAG 2 cut(s) 56, 530
StyI CCWWGG 2 cut(s) 55, 504
TaaI ACNGT 2 cut(s) 142, 545
TaiI ACGT 1 cut(s) 419
TaqI TCGA 4 cut(s) 192, 369, 582, 604
TasI AATT 4 cut(s) 13, 95, 162, 588
TatI WGTACW 3 cut(s) 201, 526, 550
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 444, 616
Tru9I TTAA 2 cut(s) 444, 616
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 171
TspRI CASTG 1 cut(s) 517
VneI GTGCAC 1 cut(s) 487
XapI RAATTY 2 cut(s) 95, 588
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 3 cut(s) 100, 216, 448
XmaJI CCTAGG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 2 cut(s) 56, 530
ZrmI AGTACT 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.