Rorug04G0097000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000735
Physical Location & Seq
Forward (+)
15261453 .. 15265348
3896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0097000.1

Sequence Viewer

Length: 735 bp
ATGGACAACATTGGTGATGAATACAAGCATTACTGGGAAACCAACATGTTCCTCCAAACCGAAGAACTCGACAGTTGGGGATTGGATGAAGCGTTTTCTGGGTACTACGATTCGAGCTCGCCGGACGGAGCAGCGTCATCCATGGCCTCCAAGAACATTGTTTCAGAGAGGAACAGGAGGAAGAAGCTCAATGAGAGGCTCTTTGCTCTTAGAGCAGTGGTCCCCAACATTAGCAAGATGGATAAGGCTTCTATAATCAAAGACGCCATTGACTACATCCAAGAGCTTCATGATCAAGAAAAAAGAATTCAAGCGGAGATCATGGATTTGGAATCTGGAAAATCAAAGAAGGGTGTTATTGGTAGTTCGGACTTCGAACAAGAGCTTCCGGTCTTGTTGAGATCGAAGAAGAAGAAAATCGAGCAGCTTTATGATTCTGGAGGATCAAGAACTTCCTCCATCGAAGTCCTTGAACTTAGGGTTACATACATGGGGGAAAAGACAGTGGTGGTGAGCTTGACATGTAGTAAGAGAACAGACACAATGGTGAAACTGTGCGAGGTCTTTGAGTCCCTGAAGCTCAAAATCATTACAGCCAATATCACTGCTTTTTCTGGGAGGGTATTGAAAACAGTCTTCATTGAGGCAGATGAAGAAGAGAAGGATCATTTGAAGGTAAAAATTGAAACAGCTATTGCAGCACTGAATGACCCACAAAGCCCCATGAGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

27.56

Weight (kDa)

5.34

Isoelectric Point (pI)

62.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 53 - 97 4.9e-12 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 158 - 233 1.2e-25 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 314
AclWI GGATC 2 cut(s) 451, 672
AcsI RAATTY 1 cut(s) 306
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 1 cut(s) 104
AflIII ACRYGT 2 cut(s) 45, 521
AgsI TTSAA 5 cut(s) 311, 473, 628, 673, 686
AleI CACNNNNGTG 1 cut(s) 545
AluBI AGCT 8 cut(s) 117, 187, 286, 385, 427, 516, 580, 692
AluI AGCT 8 cut(s) 117, 187, 286, 385, 427, 516, 580, 692
Alw21I GWGCWC 1 cut(s) 119
AlwI GGATC 2 cut(s) 451, 672
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 3 cut(s) 131, 424, 698
ApoI RAATTY 1 cut(s) 306
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 3 cut(s) 26, 523, 559
AsuII TTCGAA 1 cut(s) 375
AvaII GGWCC 1 cut(s) 220
BanII GRGCYC 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 628
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 3 cut(s) 143, 436, 710
BccI CCATC 2 cut(s) 232, 467
BclI TGATCA 1 cut(s) 292
BisI GCNGC 3 cut(s) 132, 425, 699
BlsI GCNGC 3 cut(s) 133, 426, 700
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 222
BmrI ACTGGG 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 628
BpmI CTGGAG 1 cut(s) 459
Bpu14I TTCGAA 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 388
Bse1I ACTGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 3 cut(s) 91, 137, 276
BseNI ACTGG 1 cut(s) 38
BseXI GCAGC 3 cut(s) 143, 436, 710
BshFI GGCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 2 cut(s) 122, 389
BslFI GGGAC 2 cut(s) 206, 556
BsmFI GGGAC 2 cut(s) 206, 556
BsnI GGCC 1 cut(s) 146
Bsp119I TTCGAA 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 5 cut(s) 292, 318, 401, 443, 664
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 1 cut(s) 314
BspANI GGCC 1 cut(s) 146
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 2 cut(s) 451, 672
BspT104I TTCGAA 1 cut(s) 375
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 5 cut(s) 292, 318, 401, 443, 664
BssNI GRCGYC 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 4 cut(s) 74, 505, 555, 634
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 2 cut(s) 209, 476
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 91, 137, 276
BstKTI GATC 5 cut(s) 295, 321, 404, 446, 667
BstMBI GATC 5 cut(s) 292, 318, 401, 443, 664
BstMWI GCNNNNNNNGC 3 cut(s) 212, 698, 726
BstNSI RCATGY 2 cut(s) 49, 525
BstV1I GCAGC 3 cut(s) 143, 436, 710
BstV2I GAAGAC 1 cut(s) 628
BsuRI GGCC 1 cut(s) 146
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 3 cut(s) 91, 137, 276
BtsI GCAGTG 2 cut(s) 222, 603
BtsIMutI CAGTG 4 cut(s) 222, 510, 603, 701
Cac8I GCNNGC 1 cut(s) 119
CciI TCATGA 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 220
CseI GACGC 2 cut(s) 123, 272
Csp6I GTAC 1 cut(s) 103
CviAII CATG 7 cut(s) 46, 142, 290, 322, 490, 522, 724
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 209, 476
DpnI GATC 5 cut(s) 294, 320, 403, 445, 666
DpnII GATC 5 cut(s) 292, 318, 401, 443, 664
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Ecl136II GAGCTC 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 596
EcoICRI GAGCTC 1 cut(s) 117
EcoRI GAATTC 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 7 cut(s) 49, 145, 293, 325, 493, 525, 727
FaqI GGGAC 2 cut(s) 206, 556
FatI CATG 7 cut(s) 45, 141, 289, 321, 489, 521, 723
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 132, 425, 699
FokI GGATG 3 cut(s) 98, 124, 263
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 3 cut(s) 132, 425, 699
GluI GCNGC 3 cut(s) 132, 425, 699
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 122, 389
HgaI GACGC 2 cut(s) 123, 272
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 7 cut(s) 49, 145, 293, 325, 493, 525, 727
HinfI GANTC 4 cut(s) 110, 332, 434, 569
HpaII CCGG 2 cut(s) 122, 389
HphI GGTGA 3 cut(s) 26, 523, 559
Hpy188I TCNGA 2 cut(s) 166, 370
Hpy188III TCNNGA 5 cut(s) 290, 296, 336, 438, 447
HpyAV CCTTC 3 cut(s) 343, 655, 667
HpyCH4III ACNGT 4 cut(s) 74, 505, 555, 634
HpyCH4V TGCA 1 cut(s) 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 698, 726
HpyF3I CTNAG 2 cut(s) 209, 476
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 7 cut(s) 49, 145, 293, 325, 493, 525, 727
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 5 cut(s) 292, 318, 401, 443, 664
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 9 cut(s) 19, 84, 135, 160, 321, 402, 423, 587, 600
Lsp1109I GCAGC 3 cut(s) 143, 436, 710
MaeIII GTNAC 1 cut(s) 481
MalI GATC 5 cut(s) 294, 320, 403, 445, 666
MboI GATC 5 cut(s) 292, 318, 401, 443, 664
MboII GAAGA 8 cut(s) 74, 193, 418, 421, 424, 628, 665, 668
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 2 cut(s) 306, 681
MlyI GAGTC 1 cut(s) 578
MslI CAYNNNNRTG 1 cut(s) 545
MspI CCGG 2 cut(s) 122, 389
MwoI GCNNNNNNNGC 3 cut(s) 212, 698, 726
NcoI CCATGG 1 cut(s) 141
NdeII GATC 5 cut(s) 292, 318, 401, 443, 664
NlaIII CATG 7 cut(s) 49, 145, 293, 325, 493, 525, 727
NlaIV GGNNCC 1 cut(s) 222
NspI RCATGY 2 cut(s) 49, 525
NspV TTCGAA 1 cut(s) 375
OliI CACNNNNGTG 1 cut(s) 545
PagI TCATGA 1 cut(s) 289
PciI ACATGT 2 cut(s) 45, 521
PfeI GAWTC 3 cut(s) 110, 332, 434
PkrI GCNGC 3 cut(s) 133, 426, 700
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
PscI ACATGT 2 cut(s) 45, 521
Psp124BI GAGCTC 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 1 cut(s) 220
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 545
SacI GAGCTC 1 cut(s) 119
SatI GCNGC 3 cut(s) 132, 425, 699
Sau3AI GATC 5 cut(s) 292, 318, 401, 443, 664
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 1 cut(s) 578
SduI GDGCHC 1 cut(s) 119
SfuI TTCGAA 1 cut(s) 375
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 545
Sse9I AATT 2 cut(s) 306, 681
SsiI CCGC 1 cut(s) 314
SstI GAGCTC 1 cut(s) 119
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 4 cut(s) 74, 505, 555, 634
TaqI TCGA 6 cut(s) 69, 113, 375, 404, 420, 462
TasI AATT 2 cut(s) 306, 681
TfiI GAWTC 3 cut(s) 110, 332, 434
TscAI CASTG 4 cut(s) 222, 510, 610, 708
TseI GCWGC 3 cut(s) 131, 424, 698
TspDTI ATGAA 5 cut(s) 33, 102, 278, 628, 666
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 4 cut(s) 222, 510, 610, 708
VpaK11BI GGWCC 1 cut(s) 220
XapI RAATTY 1 cut(s) 306
XceI RCATGY 2 cut(s) 49, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.