Rorug05G0453100

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000735
Physical Location & Seq
Reverse (-)
62648949 .. 62650234
1286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0453100.1

Sequence Viewer

Length: 627 bp
ATGGCAGTCACCATGAAGATTATGTGTGTGCTTGTTGCAGCACTGGGTATTCTTTCGTTTGTGTTTGGTATTCTCGCTGAAACTAAGAAGCCTGCAGCTGGAGTTCCCATAATAGGGAAAGGCATGGTGATTTGCAAGTACCCATCTGATCCAACGCTTGCTTATGCGTGGCTATCTTTTGGATTTCTTCTGTTATGTTCTGCGGTTGGTTGGTTCTCTATATTTTACAGCTACAAAGGCAGAAAAGCTCCCACATCTGTTTTCTTCCGGAGCTCTACCTTCTTCTCCTTCTTCACCATTACCCTGGGTACAATAGGATTAGCAGCTGCATTTTTGCTATGGCCTATAGCGACGCATCATCACCCAAATTATAACATTACTGGGAATCAAGACCCCCACAGCGCCACATGCCCAACTGCCAAAACTGGTGTTCTTGGCGGTGGAGCCTTCTTGTCCTACAACTCATCGCTACTCTGGTTAGTTTGCTTCATCTTGGCTAGCAATGCAAGAGAAGACTACTTTGAAGACTTGGATAGTGTCGAAAAACCTAAACACATTGAGAAAGCTGAAGCTGGACATACCACGGCACTTGAAGATGATTACAGCAGCGAGATTATCTCCCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

208

Amino Acids

22.58

Weight (kDa)

6.57

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 155 6.3e-10 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 2 cut(s) 203, 438
AclWI GGATC 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 2 cut(s) 140, 310
AfiI CCNNNNNNNGG 3 cut(s) 98, 113, 114
AgsI TTSAA 2 cut(s) 524, 593
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 7 cut(s) 98, 231, 248, 273, 326, 566, 572
AluI AGCT 7 cut(s) 98, 231, 248, 273, 326, 566, 572
Alw21I GWGCWC 1 cut(s) 275
AlwI GGATC 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 5 cut(s) 38, 95, 323, 326, 606
AspLEI GCGC 1 cut(s) 404
AsuHPI GGTGA 3 cut(s) 139, 286, 353
AsuNHI GCTAGC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 275
BbsI GAAGAC 2 cut(s) 519, 531
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 5 cut(s) 50, 107, 313, 335, 618
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 600
BciT130I CCWGG 1 cut(s) 305
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 2 cut(s) 93, 345
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 5 cut(s) 39, 96, 324, 327, 607
BlsI GCNGC 5 cut(s) 40, 97, 325, 328, 608
Bme1390I CCNGG 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 305
BmrI ACTGGG 2 cut(s) 53, 390
BmsI GCATC 1 cut(s) 364
BmtI GCTAGC 1 cut(s) 501
BmuI ACTGGG 2 cut(s) 53, 390
BpiI GAAGAC 2 cut(s) 519, 531
BplI GAGNNNNNCTC 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 303, 304, 582
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 113, 114
Bse1I ACTGG 3 cut(s) 48, 385, 430
Bse3DI GCAATG 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 303, 304, 582
BseLI CCNNNNNNNGG 3 cut(s) 98, 113, 114
BseMI GCAATG 1 cut(s) 508
BseNI ACTGG 3 cut(s) 48, 385, 430
BseXI GCAGC 5 cut(s) 50, 107, 313, 335, 618
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 268
BslI CCNNNNNNNGG 3 cut(s) 98, 113, 114
BsnI GGCC 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 275
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 2 cut(s) 203, 438
BspANI GGCC 1 cut(s) 343
BspEI TCCGGA 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 3 cut(s) 48, 385, 430
BssECI CCNNGG 3 cut(s) 303, 304, 582
BssMI GATC 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 305
BstC8I GCNNGC 3 cut(s) 93, 159, 499
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 582
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 237, 408, 503
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 303
BstSFI CTRYAG 2 cut(s) 93, 345
BstV1I GCAGC 5 cut(s) 50, 107, 313, 335, 618
BstV2I GAAGAC 2 cut(s) 519, 531
BstXI CCANNNNNNTGG 1 cut(s) 304
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 582
BtgZI GCGATG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 3 cut(s) 93, 159, 499
CfoI GCGC 1 cut(s) 404
CseI GACGC 1 cut(s) 361
Csp6I GTAC 2 cut(s) 139, 309
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 3 cut(s) 13, 124, 408
CviQI GTAC 2 cut(s) 139, 309
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 303
EcoT38I GRGCYC 1 cut(s) 275
FaeI CATG 3 cut(s) 16, 127, 411
FatI CATG 3 cut(s) 12, 123, 407
Fnu4HI GCNGC 5 cut(s) 39, 96, 324, 327, 607
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 5 cut(s) 39, 96, 324, 327, 607
FspBI CTAG 1 cut(s) 498
GlaI GCGC 1 cut(s) 403
GluI GCNGC 5 cut(s) 39, 96, 324, 327, 607
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 268
HgaI GACGC 1 cut(s) 361
HhaI GCGC 1 cut(s) 404
Hin1II CATG 3 cut(s) 16, 127, 411
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 268
HphI GGTGA 3 cut(s) 139, 286, 353
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 268, 389
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 3 cut(s) 289, 298, 457
HpyCH4V TGCA 5 cut(s) 38, 95, 135, 329, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 237, 408, 503
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 3 cut(s) 16, 127, 411
HspAI GCGC 1 cut(s) 402
Kpn2I TCCGGA 1 cut(s) 267
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 253, 270, 443
Lsp1109I GCAGC 5 cut(s) 50, 107, 313, 335, 618
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 498
MaeIII GTNAC 1 cut(s) 7
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 8 cut(s) 28, 179, 256, 274, 283, 524, 536, 605
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 176
MroI TCCGGA 1 cut(s) 267
MspA1I CMGCKG 2 cut(s) 98, 326
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 1 cut(s) 305
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 3 cut(s) 237, 408, 503
NdeII GATC 1 cut(s) 148
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 3 cut(s) 16, 127, 411
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 411
PasI CCCWGGG 1 cut(s) 304
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 5 cut(s) 40, 97, 325, 328, 608
PsiI TTATAA 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 445
PstI CTGCAG 1 cut(s) 97
PvuII CAGCTG 2 cut(s) 98, 326
RsaI GTAC 2 cut(s) 140, 310
RsaNI GTAC 2 cut(s) 139, 309
SacI GAGCTC 1 cut(s) 275
SatI GCNGC 5 cut(s) 39, 96, 324, 327, 607
Sau3AI GATC 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 275
SetI ASST 9 cut(s) 100, 233, 250, 275, 281, 328, 550, 568, 574
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 2 cut(s) 93, 345
Sse9I AATT 1 cut(s) 367
SsiI CCGC 2 cut(s) 203, 438
SspMI CTAG 1 cut(s) 498
SstI GAGCTC 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 303
TaqI TCGA 1 cut(s) 540
TasI AATT 1 cut(s) 367
TfiI GAWTC 1 cut(s) 385
TscAI CASTG 1 cut(s) 48
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 5 cut(s) 38, 95, 323, 326, 606
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 29, 478
TspRI CASTG 1 cut(s) 48
XceI RCATGY 1 cut(s) 411
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.