Rorug05G0315200

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000735
Physical Location & Seq
Forward (+)
37427677 .. 37428602
926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0315200.1

Sequence Viewer

Length: 438 bp
ATGAGTAAGCTTCAGAGTGATTCTCTTAGGAATGCTATTTCTATCATCAAGGCCGCTTCCAAGGAAAAGAGTCGTAAGTTAACAGAAACTACAGAACTTCAGATTGGGCTGAAAAATTATGATCCCCAAAAGGACAAGCGTTACAGTGGTTTCGTTAAGTTACCACACATTCCTCGTCCAAAGATGAAGTTCTGCATGCTTGGAGATGCTCAGCATGTTGAAGAGGCTCAGAAGATCGGTTTGGAGTACATGGATGTAGAAGCGCTGAAGAAGTTAAACAAGAACAAGAAATTGGTTAAGAAACTGGCTAAAAAGTACCATGCCTCTTTAGCATCAGAAGCTGTTATCAAGCAGATTCCCCGTCTTTTGGGTCCCGGTCTCAACAAGGCATGTTTTTTTAGTGCTGATTGCTTTGGTAAAAGGCAGGTTCGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

145

Amino Acids

16.43

Weight (kDa)

9.95

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 21 - 129 2.4e-22 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 415
AciI CCGC 1 cut(s) 54
AclWI GGATC 1 cut(s) 116
AcuI CTGAAG 2 cut(s) 83, 287
AfaI GTAC 3 cut(s) 248, 317, 433
AfeI AGCGCT 1 cut(s) 264
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 1 cut(s) 221
AjuI GAANNNNNNNTTGG 6 cut(s) 87, 119, 224, 256, 275, 307
AluBI AGCT 2 cut(s) 10, 341
AluI AGCT 2 cut(s) 10, 341
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 116
AlwNI CAGNNNCTG 1 cut(s) 341
Aor51HI AGCGCT 1 cut(s) 264
AoxI GGCC 1 cut(s) 51
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 1 cut(s) 375
AvaII GGWCC 1 cut(s) 371
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 383
BfmI CTRYAG 1 cut(s) 90
BfoI RGCGCY 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 415
BisI GCNGC 1 cut(s) 54
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 1 cut(s) 375
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 2 cut(s) 372, 373
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 2 cut(s) 196, 341
BplI GAGNNNNNCTC 2 cut(s) 7, 39
Bpu1102I GCTNAGC 1 cut(s) 210
BpuMI CCSGG 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 60
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMII CTCAG 2 cut(s) 224, 242
BseNI ACTGG 1 cut(s) 309
BshFI GGCC 1 cut(s) 53
BsiSI CCGG 1 cut(s) 375
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 1 cut(s) 53
Bso31I GGTCTC 1 cut(s) 383
Bsp143I GATC 2 cut(s) 121, 234
Bsp1720I GCTNAGC 1 cut(s) 210
BspACI CCGC 1 cut(s) 54
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 2 cut(s) 223, 241
BspLI GGNNCC 2 cut(s) 372, 373
BspMI ACCTGC 1 cut(s) 415
BspPI GGATC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 383
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 2 cut(s) 121, 234
BssT1I CCWWGG 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 3 cut(s) 26, 210, 228
BstF5I GGATG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 2 cut(s) 124, 237
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 2 cut(s) 121, 234
BstMWI GCNNNNNNNGC 2 cut(s) 329, 338
BstNSI RCATGY 3 cut(s) 199, 218, 393
BstSCI CCNGG 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 90
BsuRI GGCC 1 cut(s) 53
BtsCI GGATG 1 cut(s) 259
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 197
CaiI CAGNNNCTG 1 cut(s) 341
CfoI GCGC 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 371
Csp6I GTAC 3 cut(s) 247, 316, 432
CviAII CATG 5 cut(s) 196, 215, 250, 320, 390
CviJI RGCY 6 cut(s) 10, 53, 109, 227, 308, 341
CviKI_1 RGCY 6 cut(s) 10, 53, 109, 227, 308, 341
CviQI GTAC 3 cut(s) 247, 316, 432
DdeI CTNAG 3 cut(s) 26, 210, 228
DpnI GATC 2 cut(s) 123, 236
DpnII GATC 2 cut(s) 121, 234
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 264
Eco57I CTGAAG 2 cut(s) 83, 287
EcoO109I RGGNCCY 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 5 cut(s) 199, 218, 253, 323, 393
FaiI YATR 7 cut(s) 120, 197, 216, 251, 321, 391, 436
FaqI GGGAC 1 cut(s) 357
FatI CATG 5 cut(s) 195, 214, 249, 319, 389
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 1 cut(s) 54
GlaI GCGC 1 cut(s) 264
GluI GCNGC 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 1 cut(s) 53
HapII CCGG 1 cut(s) 375
HhaI GCGC 1 cut(s) 265
Hin1II CATG 5 cut(s) 199, 218, 253, 323, 393
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 3 cut(s) 20, 70, 355
HpaI GTTAAC 1 cut(s) 81
HpaII CCGG 1 cut(s) 375
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 4 cut(s) 15, 102, 231, 337
Hpy8I GTNNAC 1 cut(s) 81
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 338
HpyF3I CTNAG 3 cut(s) 26, 210, 228
Hsp92II CATG 5 cut(s) 199, 218, 253, 323, 393
HspAI GCGC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 371
KspAI GTTAAC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 121, 234
LpnPI CCDG 3 cut(s) 290, 388, 410
LweI GCATC 2 cut(s) 196, 341
MaeIII GTNAC 2 cut(s) 140, 159
MalI GATC 2 cut(s) 123, 236
MboI GATC 2 cut(s) 121, 234
MboII GAAGA 3 cut(s) 233, 244, 280
MluCI AATT 2 cut(s) 115, 290
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 3 cut(s) 183, 217, 334
MseI TTAA 4 cut(s) 80, 156, 275, 297
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 375
Mva1269I GAATGC 1 cut(s) 37
MwoI GCNNNNNNNGC 2 cut(s) 329, 338
NciI CCSGG 1 cut(s) 375
NdeII GATC 2 cut(s) 121, 234
NlaIII CATG 5 cut(s) 199, 218, 253, 323, 393
NlaIV GGNNCC 2 cut(s) 372, 373
NspI RCATGY 3 cut(s) 199, 218, 393
PaeI GCATGC 1 cut(s) 199
PctI GAATGC 1 cut(s) 37
PfeI GAWTC 2 cut(s) 20, 355
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PpuMI RGGWCCY 1 cut(s) 371
Psp5II RGGWCCY 1 cut(s) 371
PspN4I GGNNCC 2 cut(s) 372, 373
PspPI GGNCC 1 cut(s) 371
PspPPI RGGWCCY 1 cut(s) 371
PstNI CAGNNNCTG 1 cut(s) 341
RsaI GTAC 3 cut(s) 248, 317, 433
RsaNI GTAC 3 cut(s) 247, 316, 432
SaqAI TTAA 4 cut(s) 80, 156, 275, 297
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 121, 234
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 375
SetI ASST 3 cut(s) 12, 343, 429
SfaNI GCATC 2 cut(s) 196, 341
SfcI CTRYAG 1 cut(s) 90
SinI GGWCC 1 cut(s) 371
SphI GCATGC 1 cut(s) 199
Sse9I AATT 2 cut(s) 115, 290
SsiI CCGC 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 1 cut(s) 146
TasI AATT 2 cut(s) 115, 290
TatI WGTACW 1 cut(s) 246
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 2 cut(s) 20, 355
Tru1I TTAA 4 cut(s) 80, 156, 275, 297
Tru9I TTAA 4 cut(s) 80, 156, 275, 297
TscAI CASTG 1 cut(s) 151
TspDTI ATGAA 1 cut(s) 200
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 371
XceI RCATGY 3 cut(s) 199, 218, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.