Prupe.2G096400_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000735
Physical Location & Seq
Reverse (-)
15174966 .. 15175730
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G096400.1

Sequence Viewer

Length: 765 bp
ATGTCGATTGAAGTTTTGGAAAGGGTGGTGGGTGACCCTCGTTTCCGGCTTACTACAGAGCTTGAAGCCGTAATTGAACCCCAAACCGAGCTGCGATCCACCAAATTCCTTCTGAACATGGAGGCTAGGGTTAATCGCCTTCACCCCTGCTGGGATTTCGAGTTTGAGGACTTGTACGAGGACAACCACATTGACCAAAAAAATTGCACCACCCTCGTCAGCGTTAAGCACGAAAAGCTCGAGATCGACCCCTTCGCGGCTGGCAATGCCTCAGAAGAGGATTCGAGGACTGCCATATCCGGCAAGCTATCTTGCTTGAGCGTGCCAGCCGCAATTTGTCAGTGGATGACAGATAAAATATTGAACGATGCCTCTTTCTATTTGTTCGTTACCAGAGGTGGTACCGGGCATAAGGTTTTGAATCTTTCGGTTTTCATCAATGTTCATGTTGTTGACACTTACGTGTGGAACGATGACTTGGGGGAGGCCGGATTAGAATTCGTGCCGTGGACGAAAAAATTGTGGGTTGACAATAAGTTGGAGAGGGTGAGAGAAGTACAAGTTTCGACTGTGTGTTCGATTTGTATGGAGGAGATTGTGGTTGGTTCGGAAGGGACTTGTATGCCATGTTCTCATCTTTTTCATGGAGCTTGCATTCTCAAGTGGCTGGACAAAAGCAGGTCTTGTCCATTGTGTCGATTTTCCATGCCTGCTTTGATGCATGAAGAGAGAGGTATGAAGAGAGATGATGGACGAAGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

255

Amino Acids

28.95

Weight (kDa)

5.24

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 669
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 1 cut(s) 401
AccII CGCG 1 cut(s) 257
AciI CCGC 2 cut(s) 257, 330
AclWI GGATC 1 cut(s) 90
AcsI RAATTY 2 cut(s) 104, 497
AfaI GTAC 3 cut(s) 176, 403, 558
AfiI CCNNNNNNNGG 2 cut(s) 151, 256
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 5 cut(s) 11, 65, 77, 364, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AleI CACNNNNGTG 1 cut(s) 461
AluBI AGCT 5 cut(s) 61, 91, 238, 307, 650
AluI AGCT 5 cut(s) 61, 91, 238, 307, 650
AlwI GGATC 1 cut(s) 90
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 91
ApoI RAATTY 2 cut(s) 104, 497
ArsI GACNNNNNNTTYG 2 cut(s) 559, 591
Asp718I GGTACC 1 cut(s) 401
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 3 cut(s) 44, 134, 559
AvaI CYCGRG 1 cut(s) 239
BanI GGYRCC 1 cut(s) 401
BbvI GCAGC 1 cut(s) 78
BccI CCATC 1 cut(s) 743
BceAI ACGGC 2 cut(s) 53, 490
BcgI CGANNNNNNTGC 2 cut(s) 196, 230
BcnI CCSGG 1 cut(s) 406
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 669
BisI GCNGC 3 cut(s) 92, 258, 330
BlsI GCNGC 3 cut(s) 93, 259, 331
Bme1390I CCNGG 1 cut(s) 406
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 358, 708
BpuEI CTTGAG 2 cut(s) 337, 644
BpuMI CCSGG 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 463
BsaJI CCNNGG 1 cut(s) 506
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 256
Bse3DI GCAATG 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 2 cut(s) 151, 256
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 1 cut(s) 285
BseRI GAGGAG 1 cut(s) 605
BseXI GCAGC 1 cut(s) 78
BseYI CCCAGC 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 1 cut(s) 488
BshNI GGYRCC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 4 cut(s) 46, 300, 405, 489
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 2 cut(s) 151, 256
BsmFI GGGAC 1 cut(s) 628
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 1 cut(s) 488
BsoBI CYCGRG 1 cut(s) 239
Bsp143I GATC 2 cut(s) 95, 243
BspACI CCGC 2 cut(s) 257, 330
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 284
BspFNI CGCG 1 cut(s) 257
BspLI GGNNCC 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 669
BspPI GGATC 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 271
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 2 cut(s) 95, 243
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 3 cut(s) 270, 720, 734
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 6 cut(s) 262, 305, 323, 327, 652, 711
BstDEI CTNAG 1 cut(s) 271
BstDSI CCRYGG 1 cut(s) 506
BstEII GGTNACC 1 cut(s) 32
BstF5I GGATG 1 cut(s) 351
BstFNI CGCG 1 cut(s) 257
BstKTI GATC 2 cut(s) 98, 246
BstMBI GATC 2 cut(s) 95, 243
BstMWI GCNNNNNNNGC 2 cut(s) 235, 266
BstPI GGTNACC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 404
BstSFI CTRYAG 1 cut(s) 54
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 488
BtgI CCRYGG 1 cut(s) 506
BtsCI GGATG 1 cut(s) 351
BtsIMutI CAGTG 1 cut(s) 347
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 6 cut(s) 262, 305, 323, 327, 652, 711
Csp6I GTAC 3 cut(s) 175, 402, 557
CviAII CATG 6 cut(s) 118, 446, 627, 644, 706, 722
CviQI GTAC 3 cut(s) 175, 402, 557
DdeI CTNAG 1 cut(s) 271
DpnI GATC 2 cut(s) 97, 245
DpnII GATC 2 cut(s) 95, 243
Eam1104I CTCTTC 3 cut(s) 270, 720, 734
EarI CTCTTC 3 cut(s) 270, 720, 734
Eco88I CYCGRG 1 cut(s) 239
Eco91I GGTNACC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 32
EcoRI GAATTC 1 cut(s) 497
EcoT22I ATGCAT 1 cut(s) 723
FaeI CATG 6 cut(s) 121, 449, 630, 647, 709, 725
FalI AAGNNNNNCTT 2 cut(s) 667, 699
FaqI GGGAC 1 cut(s) 628
FatI CATG 6 cut(s) 117, 445, 626, 643, 705, 721
Fnu4HI GCNGC 3 cut(s) 92, 258, 330
FokI GGATG 1 cut(s) 358
Fsp4HI GCNGC 3 cut(s) 92, 258, 330
FspBI CTAG 1 cut(s) 126
GluI GCNGC 3 cut(s) 92, 258, 330
GsaI CCCAGC 1 cut(s) 154
HaeIII GGCC 1 cut(s) 488
HapII CCGG 4 cut(s) 46, 300, 405, 489
Hin1II CATG 6 cut(s) 121, 449, 630, 647, 709, 725
HincII GTYRAC 2 cut(s) 454, 529
HindII GTYRAC 2 cut(s) 454, 529
HinfI GANTC 2 cut(s) 281, 421
HpaII CCGG 4 cut(s) 46, 300, 405, 489
HphI GGTGA 3 cut(s) 44, 134, 559
Hpy166II GTNNAC 3 cut(s) 454, 510, 529
Hpy188I TCNGA 3 cut(s) 114, 274, 610
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 3 cut(s) 454, 510, 529
HpyAV CCTTC 4 cut(s) 119, 149, 262, 605
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 3 cut(s) 207, 654, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 266
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 6 cut(s) 121, 449, 630, 647, 709, 725
KpnI GGTACC 1 cut(s) 405
Kzo9I GATC 2 cut(s) 95, 243
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 1 cut(s) 78
LweI GCATC 2 cut(s) 358, 708
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 2 cut(s) 32, 388
MalI GATC 2 cut(s) 97, 245
MboI GATC 2 cut(s) 95, 243
MboII GAAGA 3 cut(s) 287, 737, 751
MluCI AATT 6 cut(s) 72, 104, 202, 333, 497, 518
MmeI TCCRAC 1 cut(s) 519
Mph1103I ATGCAT 1 cut(s) 723
MseI TTAA 2 cut(s) 132, 225
MslI CAYNNNNRTG 1 cut(s) 461
MspI CCGG 4 cut(s) 46, 300, 405, 489
MspR9I CCNGG 1 cut(s) 406
Mva1269I GAATGC 1 cut(s) 654
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 2 cut(s) 235, 266
NciI CCSGG 1 cut(s) 406
NdeII GATC 2 cut(s) 95, 243
NlaIII CATG 6 cut(s) 121, 449, 630, 647, 709, 725
NlaIV GGNNCC 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 723
OliI CACNNNNGTG 1 cut(s) 461
PaeR7I CTCGAG 1 cut(s) 239
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 468
PctI GAATGC 1 cut(s) 654
PfeI GAWTC 2 cut(s) 281, 421
PkrI GCNGC 3 cut(s) 93, 259, 331
Ppu21I YACGTR 1 cut(s) 463
PspEI GGTNACC 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 403
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
RsaI GTAC 3 cut(s) 176, 403, 558
RsaNI GTAC 3 cut(s) 175, 402, 557
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 2 cut(s) 132, 225
SatI GCNGC 3 cut(s) 92, 258, 330
Sau3AI GATC 2 cut(s) 95, 243
ScrFI CCNGG 1 cut(s) 406
SfaNI GCATC 2 cut(s) 358, 708
SfcI CTRYAG 1 cut(s) 54
Sfr274I CTCGAG 1 cut(s) 239
SlaI CTCGAG 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 3 cut(s) 239, 316, 659
SmoI CTYRAG 3 cut(s) 239, 316, 659
Sse9I AATT 6 cut(s) 72, 104, 202, 333, 497, 518
SsiI CCGC 2 cut(s) 257, 330
SspI AATATT 1 cut(s) 360
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 1 cut(s) 465
TaqI TCGA 8 cut(s) 5, 159, 240, 246, 284, 566, 578, 697
TasI AATT 6 cut(s) 72, 104, 202, 333, 497, 518
TatI WGTACW 1 cut(s) 556
TauI GCSGC 2 cut(s) 260, 332
TfiI GAWTC 2 cut(s) 281, 421
Tru1I TTAA 2 cut(s) 132, 225
Tru9I TTAA 2 cut(s) 132, 225
TscAI CASTG 1 cut(s) 347
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 5 cut(s) 424, 434, 632, 738, 752
TspRI CASTG 1 cut(s) 347
XapI RAATTY 2 cut(s) 104, 497
XhoI CTCGAG 1 cut(s) 239
XspI CTAG 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.