FvH4_6g45560

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000735
Physical Location & Seq
Reverse (-)
34954509 .. 34955256
748 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45560.t1

Sequence Viewer

Length: 690 bp
ATGGCCATGGCCATGGCCAGTGACTATTGGGAAGTGGTGGGTGATGCTCTCTGCGACGTACGCGTCGTCGGCTTCACTGAACTAGAAAACGAGTTGCTATGCAACTACACCAATACGTTCTTGATGGAATTTGAGGCGGTGTTCAGACGTGTCACCTCCGAAGAGGCAGACCGACTCCTAGATGACGACATCATCCCCCGCCAGATCGTGAATATGACCTCAATCGAACTTGACGACTCTGACTCATCCTACATGGATGCAGTCTTATCCGAAACGCTTCAACAACTGCAAGTCCCTCTGGAGGAGCGTAGCTGCATGGTGAAGAAAGTAATGTTCAGAGCCTCAGAGGCGGCACAGAATGCCGGTGGAAAGAGAGTGCGCATGCGGGTGGACATCGATTTGTATGTGGTCGATGATGATATTGATGAGGAAGCAAATGGTGGTGTTGGTGGTATAATCGGTGATTCTTATGAGCCTAGATTTGTTCCGGCGAGTACAATGTCAATTGAGAAACTGGAGAGGACGAGAGTCCAAGTGCCATCAACAATGTGTTCGGTTTGTATGGAGGAGATGATGCTTGGGTTCGAAGCAACTAAAATGCCTTGTTTACATCTTTACCATGAAGCATGCATTGTGGAGTGGCTGCAGCAAAGTGGGGTTTGCCCTTTGTGCAAGTTTCGCATGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

230

Amino Acids

25.9

Weight (kDa)

4.35

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 183 - 225 8.9e-12 Ring finger domain
zf-C3HC4_2 PF13923 183 - 224 7.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 184 - 224 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 184 - 211 2.2e-06 RING-like zinc finger
zf-rbx1 PF12678 197 - 225 8.2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
Acc16I TGCGCA 1 cut(s) 380
AccII CGCG 1 cut(s) 63
AciI CCGC 4 cut(s) 137, 199, 350, 385
AcoI YGGCCR 3 cut(s) 3, 9, 15
AcsI RAATTY 1 cut(s) 128
AfaI GTAC 2 cut(s) 60, 496
AfiI CCNNNNNNNGG 1 cut(s) 301
AflIII ACRYGT 2 cut(s) 61, 148
AgsI TTSAA 1 cut(s) 281
AjiI CACGTC 1 cut(s) 149
AluBI AGCT 1 cut(s) 312
AluI AGCT 1 cut(s) 312
AoxI GGCC 3 cut(s) 3, 9, 15
ApeKI GCWGC 3 cut(s) 312, 643, 646
ApoI RAATTY 1 cut(s) 128
Asp700I GAANNNNTTC 1 cut(s) 276
AspLEI GCGC 1 cut(s) 381
AsuHPI GGTGA 4 cut(s) 53, 145, 331, 473
AsuII TTCGAA 1 cut(s) 585
BalI TGGCCA 3 cut(s) 5, 11, 17
BbvI GCAGC 3 cut(s) 299, 630, 658
BccI CCATC 2 cut(s) 118, 547
BfaI CTAG 4 cut(s) 83, 179, 477, 688
BfmI CTRYAG 1 cut(s) 644
BglI GCCNNNNNGGC 1 cut(s) 347
BisI GCNGC 4 cut(s) 313, 351, 644, 647
BlsI GCNGC 4 cut(s) 314, 352, 645, 648
BmgBI CACGTC 1 cut(s) 149
BmsI GCATC 3 cut(s) 34, 247, 564
BoxI GACNNNNGTC 1 cut(s) 527
BpmI CTGGAG 2 cut(s) 320, 536
Bpu14I TTCGAA 1 cut(s) 585
Bsa29I ATCGAT 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 6, 12
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 2 cut(s) 18, 519
BseCI ATCGAT 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 6, 12
BseGI GGATG 3 cut(s) 192, 245, 262
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 18, 519
BseRI GAGGAG 2 cut(s) 317, 581
BseXI GCAGC 3 cut(s) 299, 630, 658
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 3 cut(s) 5, 11, 17
BshVI ATCGAT 1 cut(s) 396
BsiSI CCGG 2 cut(s) 363, 488
BsiWI CGTACG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 3 cut(s) 5, 11, 17
Bsp119I TTCGAA 1 cut(s) 585
Bsp143I GATC 1 cut(s) 204
Bsp19I CCATGG 2 cut(s) 6, 12
BspACI CCGC 4 cut(s) 137, 199, 350, 385
BspANI GGCC 3 cut(s) 5, 11, 17
BspCNI CTCAG 1 cut(s) 356
BspDI ATCGAT 1 cut(s) 396
BspFNI CGCG 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 648
BspT104I TTCGAA 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 2 cut(s) 18, 519
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 2 cut(s) 6, 12
BssMI GATC 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 6, 12
Bst6I CTCTTC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 585
BstC8I GCNNGC 3 cut(s) 383, 628, 683
BstDEI CTNAG 1 cut(s) 343
BstDSI CCRYGG 2 cut(s) 6, 12
BstF5I GGATG 3 cut(s) 192, 245, 262
BstFNI CGCG 1 cut(s) 63
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 1 cut(s) 207
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 6 cut(s) 60, 69, 347, 359, 669, 678
BstNSI RCATGY 3 cut(s) 385, 630, 685
BstPAI GACNNNNGTC 1 cut(s) 527
BstSFI CTRYAG 1 cut(s) 644
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 3 cut(s) 299, 630, 658
BstXI CCANNNNNNTGG 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 396
BsuRI GGCC 3 cut(s) 5, 11, 17
BsuTUI ATCGAT 1 cut(s) 396
BtgI CCRYGG 2 cut(s) 6, 12
BtrI CACGTC 1 cut(s) 149
BtsCI GGATG 3 cut(s) 192, 245, 262
BtsIMutI CAGTG 2 cut(s) 25, 75
Cac8I GCNNGC 3 cut(s) 383, 628, 683
CfoI GCGC 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 362
ClaI ATCGAT 1 cut(s) 396
CseI GACGC 1 cut(s) 52
Csp6I GTAC 2 cut(s) 59, 495
CviAII CATG 8 cut(s) 7, 13, 253, 316, 382, 620, 627, 682
CviJI RGCY 8 cut(s) 5, 11, 17, 72, 312, 341, 475, 643
CviKI_1 RGCY 8 cut(s) 5, 11, 17, 72, 312, 341, 475, 643
CviQI GTAC 2 cut(s) 59, 495
DdeI CTNAG 1 cut(s) 343
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 3 cut(s) 3, 9, 15
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco130I CCWWGG 2 cut(s) 6, 12
EcoT14I CCWWGG 2 cut(s) 6, 12
EcoT22I ATGCAT 1 cut(s) 632
ErhI CCWWGG 2 cut(s) 6, 12
FaeI CATG 8 cut(s) 10, 16, 256, 319, 385, 623, 630, 685
FaqI GGGAC 1 cut(s) 278
FatI CATG 8 cut(s) 6, 12, 252, 315, 381, 619, 626, 681
FauI CCCGC 2 cut(s) 206, 378
Fnu4HI GCNGC 4 cut(s) 313, 351, 644, 647
FokI GGATG 3 cut(s) 179, 232, 269
Fsp4HI GCNGC 4 cut(s) 313, 351, 644, 647
FspAI RTGCGCAY 1 cut(s) 380
FspBI CTAG 4 cut(s) 83, 179, 477, 688
FspI TGCGCA 1 cut(s) 380
GlaI GCGC 1 cut(s) 380
GluI GCNGC 4 cut(s) 313, 351, 644, 647
GsuI CTGGAG 2 cut(s) 320, 536
HaeIII GGCC 3 cut(s) 5, 11, 17
HapII CCGG 2 cut(s) 363, 488
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 381
Hin1II CATG 8 cut(s) 10, 16, 256, 319, 385, 623, 630, 685
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HinfI GANTC 5 cut(s) 174, 236, 242, 464, 528
HpaII CCGG 2 cut(s) 363, 488
HphI GGTGA 4 cut(s) 53, 145, 331, 473
Hpy166II GTNNAC 2 cut(s) 391, 608
Hpy188I TCNGA 6 cut(s) 146, 160, 241, 271, 338, 346
Hpy188III TCNNGA 3 cut(s) 121, 208, 299
Hpy8I GTNNAC 2 cut(s) 391, 608
Hpy99I CGWCG 3 cut(s) 59, 68, 71
HpyCH4IV ACGT 3 cut(s) 57, 116, 148
HpyCH4V TGCA 7 cut(s) 102, 260, 289, 315, 630, 646, 672
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 69, 347, 359, 669, 678
HpyF3I CTNAG 1 cut(s) 343
HpySE526I ACGT 3 cut(s) 57, 116, 148
Hsp92II CATG 8 cut(s) 10, 16, 256, 319, 385, 623, 630, 685
HspAI GCGC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 304
LpnPI CCDG 6 cut(s) 31, 215, 284, 376, 500, 501
Lsp1109I GCAGC 3 cut(s) 299, 630, 658
LweI GCATC 3 cut(s) 34, 247, 564
MaeI CTAG 4 cut(s) 83, 179, 477, 688
MaeII ACGT 3 cut(s) 57, 116, 148
MaeIII GTNAC 2 cut(s) 20, 151
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 2 cut(s) 173, 334
MfeI CAATTG 1 cut(s) 504
MlsI TGGCCA 3 cut(s) 5, 11, 17
MluCI AATT 2 cut(s) 128, 504
MluI ACGCGT 1 cut(s) 61
MluNI TGGCCA 3 cut(s) 5, 11, 17
MlyI GAGTC 4 cut(s) 168, 230, 236, 537
Mox20I TGGCCA 3 cut(s) 5, 11, 17
Mph1103I ATGCAT 1 cut(s) 632
MroXI GAANNNNTTC 1 cut(s) 276
MscI TGGCCA 3 cut(s) 5, 11, 17
MslI CAYNNNNRTG 2 cut(s) 11, 386
Msp20I TGGCCA 3 cut(s) 5, 11, 17
MspI CCGG 2 cut(s) 363, 488
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 364
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 6 cut(s) 60, 69, 347, 359, 669, 678
NcoI CCATGG 2 cut(s) 6, 12
NdeII GATC 1 cut(s) 204
NlaIII CATG 8 cut(s) 10, 16, 256, 319, 385, 623, 630, 685
NmuCI GTSAC 2 cut(s) 20, 151
NsbI TGCGCA 1 cut(s) 380
NsiI ATGCAT 1 cut(s) 632
NspI RCATGY 3 cut(s) 385, 630, 685
NspV TTCGAA 1 cut(s) 585
PaeI GCATGC 3 cut(s) 385, 630, 685
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 231
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 464
Pfl23II CGTACG 1 cut(s) 58
PkrI GCNGC 4 cut(s) 314, 352, 645, 648
PleI GAGTC 4 cut(s) 168, 230, 236, 536
PpsI GAGTC 4 cut(s) 168, 230, 236, 536
PshAI GACNNNNGTC 1 cut(s) 527
PspLI CGTACG 1 cut(s) 58
PstI CTGCAG 1 cut(s) 648
RsaI GTAC 2 cut(s) 60, 496
RsaNI GTAC 2 cut(s) 59, 495
RseI CAYNNNNRTG 2 cut(s) 11, 386
SatI GCNGC 4 cut(s) 313, 351, 644, 647
Sau3AI GATC 1 cut(s) 204
SchI GAGTC 4 cut(s) 168, 230, 236, 537
SetI ASST 6 cut(s) 60, 119, 151, 158, 221, 314
SfaNI GCATC 3 cut(s) 34, 247, 564
SfcI CTRYAG 1 cut(s) 644
SfuI TTCGAA 1 cut(s) 585
SmiMI CAYNNNNRTG 2 cut(s) 11, 386
SphI GCATGC 3 cut(s) 385, 630, 685
Sse9I AATT 2 cut(s) 128, 504
SsiI CCGC 4 cut(s) 137, 199, 350, 385
SspMI CTAG 4 cut(s) 83, 179, 477, 688
StyI CCWWGG 2 cut(s) 6, 12
TaiI ACGT 3 cut(s) 60, 119, 151
TaqI TCGA 4 cut(s) 225, 396, 411, 585
TaqII GACCGA 1 cut(s) 186
TasI AATT 2 cut(s) 128, 504
TatI WGTACW 1 cut(s) 494
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 1 cut(s) 464
TscAI CASTG 2 cut(s) 25, 82
TseFI GTSAC 2 cut(s) 20, 151
TseI GCWGC 3 cut(s) 312, 643, 646
Tsp45I GTSAC 2 cut(s) 20, 151
TspDTI ATGAA 1 cut(s) 636
TspRI CASTG 2 cut(s) 25, 82
XapI RAATTY 1 cut(s) 128
XceI RCATGY 3 cut(s) 385, 630, 685
XmnI GAANNNNTTC 1 cut(s) 276
XspI CTAG 4 cut(s) 83, 179, 477, 688
Zsp2I ATGCAT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.