MD02G1237700.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000735
Physical Location & Seq
Reverse (-)
28542692 .. 28543387
696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1237700.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGCCAATCACGGAAGGGAATTAGTGGTGGGTGAGCCTTATTGTGATACCTGGGTTCATGGAATTGATGAGTTTGAAGACAACGAACGAAGCATGCATTTTGTGATGTATATCAAGGCAACAATCAGACGCCACCAGCCTAGGGTTTCAGAGGAAGTGGAAGAAGAGGACGCAGCAATAGAAGAACACTTGAGCGTGAATGATGCCACAATGGAACTACAATTTGACCCAGAGAGTGACATGGGTCTTCTCATATCCAACACACTTTCAGGCCTTCATATTCCTCTCCAGGCTCATCCACCCATAATAGCTGAAATATTGTATGAAGCCTACTCCTGGGCCCTGGAAAATGATACTTATTTTCCAGGTCGAGAGGTTTTTCATATGGGGGTGTACGTCGAAGTCAGTGTTTATCGGACATACTTGTCAGATATTCGGGACATCAACATGGACGTAGAGGATGGTGGTGCTGAGCCTCAATTTGTACCGGCAAGCAAATCGTCCATTGAGGAGTTGGAGAGGACGACAGTCAATGCCACAACAATGTGTTCTATATGCAGGGAGGAGATGATGGTTGGCTCAGAAGCAACTCGGATGCCGTGCTCACATCTTTACCATGGAGATTGTATTGTGGAGTGGCTGCAGAGAAGCAGGAATTGCCCGTTGTGTAGGTACTCCATGCCTGCTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

232

Amino Acids

26.46

Weight (kDa)

4.53

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 182 - 224 2.6e-14 Ring finger domain
zf-C3HC4_2 PF13923 182 - 223 4.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 183 - 210 1.9e-08 RING-like zinc finger
zf-C3HC4 PF00097 183 - 223 2.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 183 - 224 7.1e-07 RING-H2 zinc finger domain
zf-RING_UBOX PF13445 183 - 221 3.7e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 424
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 130
AfaI GTAC 3 cut(s) 395, 486, 674
AfiI CCNNNNNNNGG 2 cut(s) 142, 336
AgsI TTSAA 1 cut(s) 77
AjnI CCWGG 5 cut(s) 50, 288, 335, 342, 364
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Alw21I GWGCWC 1 cut(s) 605
AoxI GGCC 3 cut(s) 3, 271, 339
ApaI GGGCCC 1 cut(s) 343
ApeKI GCWGC 2 cut(s) 173, 640
AspA2I CCTAGG 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 339, 340
AsuHPI GGTGA 1 cut(s) 44
AvrII CCTAGG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 343
BaeI ACNNNNGTAYC 2 cut(s) 39, 72
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 343
BbsI GAAGAC 2 cut(s) 84, 239
Bbv12I GWGCWC 1 cut(s) 605
BbvI GCAGC 2 cut(s) 185, 627
BccI CCATC 2 cut(s) 455, 565
BceAI ACGGC 1 cut(s) 583
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 5 cut(s) 52, 290, 337, 344, 366
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 641
BisI GCNGC 2 cut(s) 174, 641
BlnI CCTAGG 1 cut(s) 140
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 2 cut(s) 175, 642
Bme1390I CCNGG 5 cut(s) 52, 290, 337, 344, 366
BmgT120I GGNCC 2 cut(s) 339, 340
BmiI GGNNCC 1 cut(s) 341
BmrFI CCNGG 5 cut(s) 52, 290, 337, 344, 366
BmsI GCATC 2 cut(s) 193, 585
BoxI GACNNNNGTC 2 cut(s) 243, 527
BpiI GAAGAC 2 cut(s) 84, 239
BpmI CTGGAG 1 cut(s) 272
Bpu1102I GCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 130
BsaJI CCNNGG 5 cut(s) 51, 140, 336, 342, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 336
Bse118I RCCGGY 1 cut(s) 487
Bse8I GATNNNNATC 1 cut(s) 110
BseBI CCWGG 5 cut(s) 52, 290, 337, 344, 366
BseDI CCNNGG 5 cut(s) 51, 140, 336, 342, 616
BseGI GGATG 3 cut(s) 295, 466, 600
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 2 cut(s) 142, 336
BseMII CTCAG 2 cut(s) 462, 594
BseRI GAGGAG 2 cut(s) 524, 578
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 2 cut(s) 185, 627
BshFI GGCC 3 cut(s) 5, 273, 341
BsiHKAI GWGCWC 1 cut(s) 605
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 142, 336
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 3 cut(s) 5, 273, 341
Bsp120I GGGCCC 1 cut(s) 339
Bsp1286I GDGCHC 2 cut(s) 343, 605
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp19I CCATGG 1 cut(s) 616
BspANI GGCC 3 cut(s) 5, 273, 341
BspCNI CTCAG 2 cut(s) 463, 593
BspLI GGNNCC 1 cut(s) 341
BspMAI CTGCAG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 487
BssAI RCCGGY 1 cut(s) 487
BssECI CCNNGG 5 cut(s) 51, 140, 336, 342, 616
BssNI GRCGYC 1 cut(s) 130
BssT1I CCWWGG 2 cut(s) 140, 616
Bst2UI CCWGG 5 cut(s) 52, 290, 337, 344, 366
Bst4CI ACNGT 1 cut(s) 529
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 130
BstAPI GCANNNNNTGC 1 cut(s) 657
BstC8I GCNNGC 3 cut(s) 95, 493, 684
BstDEI CTNAG 2 cut(s) 471, 580
BstDSI CCRYGG 1 cut(s) 616
BstF5I GGATG 3 cut(s) 295, 466, 600
BstMWI GCNNNNNNNGC 1 cut(s) 657
BstNI CCWGG 5 cut(s) 52, 290, 337, 344, 366
BstNSI RCATGY 1 cut(s) 97
BstPAI GACNNNNGTC 2 cut(s) 243, 527
BstSCI CCNGG 5 cut(s) 50, 288, 335, 342, 364
BstSFI CTRYAG 1 cut(s) 641
BstSLI GKGCMC 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 185, 627
BstV2I GAAGAC 2 cut(s) 84, 239
BsuRI GGCC 3 cut(s) 5, 273, 341
BtgI CCRYGG 1 cut(s) 616
BtsCI GGATG 3 cut(s) 295, 466, 600
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 3 cut(s) 95, 493, 684
Cfr10I RCCGGY 1 cut(s) 487
Cfr13I GGNCC 2 cut(s) 339, 340
CseI GACGC 2 cut(s) 138, 179
Csp6I GTAC 3 cut(s) 394, 485, 673
CviAII CATG 6 cut(s) 59, 94, 241, 448, 617, 679
CviQI GTAC 3 cut(s) 394, 485, 673
DdeI CTNAG 2 cut(s) 471, 580
DrdI GACNNNNNNGTC 1 cut(s) 424
DseDI GACNNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 140, 616
Eco147I AGGCCT 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 5 cut(s) 50, 288, 335, 342, 364
EcoT14I CCWWGG 2 cut(s) 140, 616
EcoT22I ATGCAT 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 2 cut(s) 140, 616
FaeI CATG 6 cut(s) 62, 97, 244, 451, 620, 682
FaqI GGGAC 1 cut(s) 452
FatI CATG 6 cut(s) 58, 93, 240, 447, 616, 678
FauNDI CATATG 1 cut(s) 384
Fnu4HI GCNGC 2 cut(s) 174, 641
FokI GGATG 3 cut(s) 282, 473, 607
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 2 cut(s) 174, 641
FspBI CTAG 1 cut(s) 141
GluI GCNGC 2 cut(s) 174, 641
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 3 cut(s) 5, 273, 341
HapII CCGG 1 cut(s) 488
HgaI GACGC 2 cut(s) 138, 179
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 6 cut(s) 62, 97, 244, 451, 620, 682
HpaII CCGG 1 cut(s) 488
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 6 cut(s) 128, 151, 417, 430, 583, 594
Hpy188III TCNNGA 2 cut(s) 371, 437
Hpy8I GTNNAC 1 cut(s) 394
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 2 cut(s) 9, 284
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 2 cut(s) 396, 453
HpyCH4V TGCA 3 cut(s) 97, 558, 643
HpyF10VI GCNNNNNNNGC 1 cut(s) 657
HpyF3I CTNAG 2 cut(s) 471, 580
HpySE526I ACGT 2 cut(s) 396, 453
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 6 cut(s) 62, 97, 244, 451, 620, 682
Lsp1109I GCAGC 2 cut(s) 185, 627
LweI GCATC 2 cut(s) 193, 585
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 396, 453
MaeIII GTNAC 1 cut(s) 236
MboII GAAGA 5 cut(s) 89, 173, 176, 194, 239
MhlI GDGCHC 2 cut(s) 343, 605
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 20, 63, 221, 479, 655
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 282, 495
MnlI CCTC 9 cut(s) 145, 160, 294, 367, 451, 486, 502, 513, 556
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 99
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 446, 542
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 5 cut(s) 52, 290, 337, 344, 366
MvaI CCWGG 5 cut(s) 52, 290, 337, 344, 366
MwoI GCNNNNNNNGC 1 cut(s) 657
NcoI CCATGG 1 cut(s) 616
NdeI CATATG 1 cut(s) 384
NlaIII CATG 6 cut(s) 62, 97, 244, 451, 620, 682
NlaIV GGNNCC 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 236
NsiI ATGCAT 1 cut(s) 99
NspI RCATGY 1 cut(s) 97
PaeI GCATGC 1 cut(s) 97
PceI AGGCCT 1 cut(s) 273
PkrI GCNGC 2 cut(s) 175, 642
PshAI GACNNNNGTC 2 cut(s) 243, 527
Psp6I CCWGG 5 cut(s) 50, 288, 335, 342, 364
PspGI CCWGG 5 cut(s) 50, 288, 335, 342, 364
PspN4I GGNNCC 1 cut(s) 341
PspOMI GGGCCC 1 cut(s) 339
PspPI GGNCC 2 cut(s) 339, 340
PstI CTGCAG 1 cut(s) 645
RsaI GTAC 3 cut(s) 395, 486, 674
RsaNI GTAC 3 cut(s) 394, 485, 673
RseI CAYNNNNRTG 2 cut(s) 446, 542
SatI GCNGC 2 cut(s) 174, 641
Sau96I GGNCC 2 cut(s) 339, 340
ScrFI CCNGG 5 cut(s) 52, 290, 337, 344, 366
SduI GDGCHC 2 cut(s) 343, 605
SetI ASST 7 cut(s) 53, 313, 370, 378, 399, 456, 674
SfaNI GCATC 2 cut(s) 193, 585
SfcI CTRYAG 1 cut(s) 641
SmiMI CAYNNNNRTG 2 cut(s) 446, 542
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
SphI GCATGC 1 cut(s) 97
Sse9I AATT 5 cut(s) 20, 63, 221, 479, 655
SseBI AGGCCT 1 cut(s) 273
SspI AATATT 1 cut(s) 318
SspMI CTAG 1 cut(s) 141
StuI AGGCCT 1 cut(s) 273
StyD4I CCNGG 5 cut(s) 50, 288, 335, 342, 364
StyI CCWWGG 2 cut(s) 140, 616
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 2 cut(s) 399, 456
TaqI TCGA 2 cut(s) 370, 399
TasI AATT 5 cut(s) 20, 63, 221, 479, 655
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 2 cut(s) 173, 640
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 4 cut(s) 47, 266, 339, 371
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 412
XceI RCATGY 1 cut(s) 97
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XmaJI CCTAGG 1 cut(s) 140
XspI CTAG 1 cut(s) 141
Zsp2I ATGCAT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.