RLG00000035226

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000735
Physical Location & Seq
Reverse (-)
63186570 .. 63187534
965 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035226

Sequence Viewer

Length: 555 bp
ATGGCTACGGAAATTTGGCAAGTGGTGGGTGACATCACCTGCAACCCAAGATACCTAGGTCTTGTTCAACCTGGAAAGGAGCTGCCTAACACCAAATTCTTGATAGATTTCAATATCAAGATGAGATACGCGCAATCTATTGATTTGGCGGGTGATGAAGAAATTGTAGTAGAGAAGAATCTCCGCTCAGTCGAACTTGAGTACAACCCACATAGCATGTGGGTTGCCATAAATAAAGAGCTTCAAGACCTGCGAGTTCCGAGCCAAGTTCGTGAAGCCATTGAAGATCCAATACTCAAGGGAACCAGAGTGGCACGAGCCGATAGCTCTTATGACGCTCGCTATTATCTGCATATGGAGGTGGATATTGATTTTCGTCTTGGCGGTTTTGATGGTATTGGTGAATATGAGCCTACGTTTGTGCCGGCAAGTGAAGCGTCCATTAAGAAACTGAAGAAGACAAGAGCCGAAGTGTCGTCGACAATGTGCACGGTCTGTATGGAGGAGATGATGGTTGGTTCGGAAGCGACTCACTTTTTTCTTAAGAAGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

185

Amino Acids

20.94

Weight (kDa)

5.08

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 2 cut(s) 47, 258
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 479
AccII CGCG 1 cut(s) 131
AciI CCGC 3 cut(s) 149, 184, 384
AclWI GGATC 1 cut(s) 281
AcsI RAATTY 2 cut(s) 12, 95
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 1 cut(s) 203
AflII CTTAAG 1 cut(s) 542
AgsI TTSAA 4 cut(s) 68, 112, 245, 284
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 3 cut(s) 82, 241, 327
AluI AGCT 3 cut(s) 82, 241, 327
Alw21I GWGCWC 1 cut(s) 491
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 1 cut(s) 281
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 2 cut(s) 12, 95
AspA2I CCTAGG 1 cut(s) 55
AspLEI GCGC 1 cut(s) 133
AsuHPI GGTGA 4 cut(s) 28, 41, 164, 413
AvrII CCTAGG 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 491
BaeI ACNNNNGTAYC 2 cut(s) 43, 76
BarI GAAGNNNNNNTAC 2 cut(s) 276, 308
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 1 cut(s) 69
BccI CCATC 2 cut(s) 386, 505
BciT130I CCWGG 1 cut(s) 72
BfaI CTAG 1 cut(s) 56
BfrI CTTAAG 1 cut(s) 542
BfuAI ACCTGC 2 cut(s) 47, 258
BisI GCNGC 1 cut(s) 83
BlnI CCTAGG 1 cut(s) 55
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 464
BpuEI CTTGAG 2 cut(s) 218, 281
BsaJI CCNNGG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 424
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 201
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 1 cut(s) 286
BspACI CCGC 3 cut(s) 149, 184, 384
BspCNI CTCAG 1 cut(s) 200
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 304
BspMI ACCTGC 2 cut(s) 47, 258
BspPI GGATC 1 cut(s) 281
BspTI CTTAAG 1 cut(s) 542
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 1 cut(s) 286
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 55
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 1 cut(s) 493
BstAFI CTTAAG 1 cut(s) 542
BstC8I GCNNGC 2 cut(s) 340, 426
BstDEI CTNAG 1 cut(s) 187
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 1 cut(s) 220
BstSCI CCNGG 1 cut(s) 70
BstSLI GKGCMC 1 cut(s) 491
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BveI ACCTGC 2 cut(s) 47, 258
Cac8I GCNNGC 2 cut(s) 340, 426
CfoI GCGC 1 cut(s) 133
Cfr10I RCCGGY 1 cut(s) 424
CseI GACGC 2 cut(s) 344, 426
Csp6I GTAC 1 cut(s) 202
CviAII CATG 1 cut(s) 217
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 187
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 1 cut(s) 220
FaiI YATR 8 cut(s) 213, 218, 230, 333, 354, 356, 408, 500
FatI CATG 1 cut(s) 216
FauI CCCGC 1 cut(s) 142
FauNDI CATATG 1 cut(s) 354
FblI GTMKAC 1 cut(s) 479
Fnu4HI GCNGC 1 cut(s) 83
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 1 cut(s) 56
GlaI GCGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 83
HapII CCGG 1 cut(s) 425
HgaI GACGC 2 cut(s) 344, 426
HhaI GCGC 1 cut(s) 133
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 2 cut(s) 178, 529
HpaII CCGG 1 cut(s) 425
HphI GGTGA 4 cut(s) 28, 41, 164, 413
Hpy166II GTNNAC 2 cut(s) 480, 489
Hpy188I TCNGA 2 cut(s) 261, 523
Hpy188III TCNNGA 4 cut(s) 100, 118, 245, 272
Hpy8I GTNNAC 2 cut(s) 480, 489
Hpy99I CGWCG 1 cut(s) 481
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 3 cut(s) 42, 352, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 1 cut(s) 187
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 1 cut(s) 131
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 6 cut(s) 52, 57, 84, 263, 319, 438
Lsp1109I GCAGC 1 cut(s) 69
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 1 cut(s) 29
MalI GATC 1 cut(s) 288
MbiI CCGCTC 1 cut(s) 186
MboI GATC 1 cut(s) 286
MboII GAAGA 5 cut(s) 170, 187, 296, 466, 469
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 3 cut(s) 12, 95, 162
MlyI GAGTC 1 cut(s) 523
MnlI CCTC 2 cut(s) 352, 496
MroNI GCCGGC 1 cut(s) 424
MseI TTAA 2 cut(s) 444, 543
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 72
MvaI CCWGG 1 cut(s) 72
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 434
NaeI GCCGGC 1 cut(s) 426
NdeI CATATG 1 cut(s) 354
NdeII GATC 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 478
SaqAI TTAA 2 cut(s) 444, 543
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 1 cut(s) 286
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 1 cut(s) 491
SmlI CTYRAG 3 cut(s) 197, 296, 542
SmoI CTYRAG 3 cut(s) 197, 296, 542
Sse9I AATT 3 cut(s) 12, 95, 162
SsiI CCGC 3 cut(s) 149, 184, 384
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 493
TaiI ACGT 1 cut(s) 419
TaqI TCGA 2 cut(s) 192, 479
TasI AATT 3 cut(s) 12, 95, 162
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 444, 543
Tru9I TTAA 2 cut(s) 444, 543
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 171
TspGWI ACGGA 1 cut(s) 23
Vha464I CTTAAG 1 cut(s) 542
VneI GTGCAC 1 cut(s) 487
XapI RAATTY 2 cut(s) 12, 95
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 216
XmaJI CCTAGG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 479
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.