Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000735

Physical Location & Seq

Genomic Coordinates Reverse (-)

33077626 .. 33078405

Gene Structure 780 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1258700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 780 bp

ATGGACACTGTGGAGGGCCAAAGTGATGAGTTCTGGGATGTGGTGGGCGATACCACATACTGTGAAACACGGCTACTCGACGTTTTGAAGTTACCCAAATCGGAGAAATATTCCAAGTTGTGGCTCGATATCAAGGCCACCGTTACAGTAATGGTGCTGCCCAGGGATTTAGAGGATGAGGAGGAGTTGGAGGACCACTACAAAGTAATGTTACTAGAAGATGATCAAATGGGACAAACCACCGACGTCGTCGAGTACGAGGAAAAACTTGAGATTGACCTTTTGATGGAGGACAATGCCACAAAAAGGACTTTGGATACCATATCTCAGGAGCTATCCAAGCTGATCCACGTCCGTTCTGGGAATCTTTTATCCGCCGCGGCTACTATATTACGGGATGCGCGTGCTGTTGCAAGAGATAAGAGTTTTGTTGTTAGCAATGGTGCTGATCAAGGTCGCACCAACCCTGTACTTGTGCATATGGAGGCATTCATCAGCATTGATGCGGTTGACGACGAGCTGCCGGTTGATGTGCAGCCTGCGTTCGTGCCGGCGAGTAAATCTTCGATTGAGAAGTTAGAGAGGGTGAAAGTGGAAGCCTCGTCGCAGGGGGTTTGTTGCTCGATTTGTCTGGCGGAGATTGTGGTTGGTTCCGAAGCACTTGGTATGCCGTGTTTGCATTTTTACCACGAGGAGTGCATTGTCGAGTGGTTGGAGAAAAGCCGGTTCTGCCCGCTGTGCCGCTTCTCAATGCCTACTGACGATCATTACTCATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008593 GO:0009056 GO:0009057 GO:0009966 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010646 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023051 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034620 GO:0034976 GO:0035264 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0040007 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048583 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0098796 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1902494 GO:1990234 GO:1990381

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

260

Amino Acids

29.16

Weight (kDa)

4.46

Isoelectric Point (pI)

43.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	207 - 248	8.6e-12	Ring finger domain
zf-RING_11	PF17123	207 - 234	2.6e-07	RING-like zinc finger
zf-C3HC4	PF00097	207 - 247	3.8e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	207 - 247	5.3e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	220 - 248	3.6e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000735)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g31481 FvH4_6g45550 FvH4_6g45551 FvH4_6g45560
malus_domestica	MD02G1237700.v1.1 MD02G1241100.v1.1 MD07G1074200.v1.1 MD07G1074300.v1.1 MD07G1077200.v1.1 MD09G1258700.v1.1
prunus_persica	Prupe.2G094100_v2.0.a1 Prupe.2G096300_v2.0.a1 Prupe.2G096400_v2.0.a1 Prupe.5G061200_v2.0.a1 Prupe.5G067900_v2.0.a1
pyrus_communis	pycom02g20840 pycom07g05830 pycom07g06070 pycom07g06080
rosa_chinensis	RchiOBHm_Chr1g0336601 RchiOBHm_Chr2g0163491 RchiOBHm_Chr5g0058471 RchiOBHm_Chr7g0236931
rosa_laevigata	RLG00000001047 RLG00000021406 RLG00000029344 RLG00000035224 RLG00000035225 RLG00000035226 RLG00000036678
rosa_multiflora	Rmu_co8414615.1_g000001 Rmu_sc0000367.1_g000124 Rmu_sc0000367.1_g000131 Rmu_sc0001969.1_g000007 Rmu_sc0004006.1_g000003 Rmu_sc0005576.1_g000003 Rmu_sc0008383.1_g000007 Rmu_sc0013124.1_g000008 Rmu_sc0024363.1_g000004 Rmu_ssc0000335.1_g000002
rosa_roxburghii	Rroxscaffold_1G00004750 Rroxscaffold_1G00022030 Rroxscaffold_1G00022050 Rroxscaffold_1G00022060 Rroxscaffold_2G00087240 Rroxscaffold_2G00087270 Rroxscaffold_3G00224680
rosa_rugosa	Rorug04G0097000 Rorug05G0315100 Rorug05G0315200 Rorug05G0315400 Rorug05G0453100 Rorug07G0301000
rosa_samantha	Rh1DG154700 Rh2AG570200 Rh2BG582400 Rh2CG552200 Rh4CG163700 Rh5AG381500 Rh5BG393100 Rh5CG417300 Rh5DG408300 Rh5DG408400 Rh5DG541900 Rh7AG456000 Rh7BG427300 Rh7CG474800 Rh7DG443900
rosa_wichuraiana	Rw1G011960 Rw2G047210 Rw5G035950 Rw7G037850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	249
AccB7I	CCANNNNNTGG	1 cut(s)	120
AccII	CGCG	2 cut(s)	380, 403
AciI	CCGC	7 cut(s)	375, 378, 380, 506, 635, 734, 742
AclWI	GGATC	1 cut(s)	340
AcyI	GRCGYC	1 cut(s)	246
AfaI	GTAC	2 cut(s)	257, 471
AfiI	CCNNNNNNNGG	3 cut(s)	120, 286, 306
AgsI	TTSAA	1 cut(s)	88
AjiI	CACGTC	1 cut(s)	352
AjnI	CCWGG	1 cut(s)	161
AluBI	AGCT	3 cut(s)	334, 343, 520
AluI	AGCT	3 cut(s)	334, 343, 520
AlwI	GGATC	1 cut(s)	340
AoxI	GGCC	2 cut(s)	16, 135
ApeKI	GCWGC	3 cut(s)	157, 520, 535
AspLEI	GCGC	1 cut(s)	403
AspS9I	GGNCC	2 cut(s)	16, 193
AsuHPI	GGTGA	1 cut(s)	598
AvaII	GGWCC	1 cut(s)	193
BauI	CACGAG	1 cut(s)	689
BbvI	GCAGC	3 cut(s)	144, 507, 547
BccI	CCATC	1 cut(s)	280
BceAI	ACGGC	2 cut(s)	86, 655
BciT130I	CCWGG	1 cut(s)	163
BciVI	GTATCC	1 cut(s)	310
BclI	TGATCA	2 cut(s)	223, 448
BfaI	CTAG	1 cut(s)	215
BfuI	GTATCC	1 cut(s)	310
BisI	GCNGC	6 cut(s)	158, 378, 381, 521, 536, 742
BlsI	GCNGC	6 cut(s)	159, 379, 382, 522, 537, 743
Bme1390I	CCNGG	1 cut(s)	163
Bme18I	GGWCC	1 cut(s)	193
BmgBI	CACGTC	1 cut(s)	352
BmgT120I	GGNCC	2 cut(s)	16, 193
BmiI	GGNNCC	1 cut(s)	652
BmrFI	CCNGG	1 cut(s)	163
BmsI	GCATC	2 cut(s)	388, 493
BpuEI	CTTGAG	1 cut(s)	290
BsaHI	GRCGYC	1 cut(s)	246
BsaJI	CCNNGG	3 cut(s)	161, 162, 378
Bsc4I	CCNNNNNNNGG	3 cut(s)	120, 286, 306
Bse118I	RCCGGY	3 cut(s)	523, 550, 723
Bse3DI	GCAATG	1 cut(s)	445
BseBI	CCWGG	1 cut(s)	163
BseDI	CCNNGG	3 cut(s)	161, 162, 378
BseGI	GGATG	3 cut(s)	43, 181, 403
BseLI	CCNNNNNNNGG	3 cut(s)	120, 286, 306
BseMI	GCAATG	1 cut(s)	445
BseMII	CTCAG	1 cut(s)	341
BseRI	GAGGAG	3 cut(s)	194, 197, 707
BseXI	GCAGC	3 cut(s)	144, 507, 547
BsgI	GTGCAG	1 cut(s)	554
Bsh1236I	CGCG	2 cut(s)	380, 403
BshFI	GGCC	2 cut(s)	18, 137
BsiSI	CCGG	3 cut(s)	524, 551, 724
BslFI	GGGAC	1 cut(s)	246
BslI	CCNNNNNNNGG	3 cut(s)	120, 286, 306
BsmFI	GGGAC	1 cut(s)	246
BsmI	GAATGC	1 cut(s)	488
BsnI	GGCC	2 cut(s)	18, 137
Bsp143I	GATC	4 cut(s)	223, 345, 448, 763
BspACI	CCGC	7 cut(s)	375, 378, 380, 506, 635, 734, 742
BspANI	GGCC	2 cut(s)	18, 137
BspCNI	CTCAG	1 cut(s)	340
BspFNI	CGCG	2 cut(s)	380, 403
BspHI	TCATGA	1 cut(s)	776
BspLI	GGNNCC	1 cut(s)	652
BspPI	GGATC	1 cut(s)	340
BsrDI	GCAATG	1 cut(s)	445
BsrFI	RCCGGY	3 cut(s)	523, 550, 723
BssAI	RCCGGY	3 cut(s)	523, 550, 723
BssECI	CCNNGG	3 cut(s)	161, 162, 378
BssMI	GATC	4 cut(s)	223, 345, 448, 763
BssNI	GRCGYC	1 cut(s)	246
BssSI	CACGAG	1 cut(s)	689
Bst2BI	CACGAG	1 cut(s)	689
Bst2UI	CCWGG	1 cut(s)	163
Bst4CI	ACNGT	4 cut(s)	10, 62, 142, 148
BstACI	GRCGYC	1 cut(s)	246
BstC8I	GCNNGC	4 cut(s)	405, 540, 552, 734
BstDEI	CTNAG	1 cut(s)	327
BstDSI	CCRYGG	1 cut(s)	378
BstF5I	GGATG	3 cut(s)	43, 181, 403
BstFNI	CGCG	2 cut(s)	380, 403
BstHHI	GCGC	1 cut(s)	403
BstKTI	GATC	4 cut(s)	226, 348, 451, 766
BstMBI	GATC	4 cut(s)	223, 345, 448, 763
BstMWI	GCNNNNNNNGC	4 cut(s)	340, 676, 729, 738
BstNI	CCWGG	1 cut(s)	163
BstSCI	CCNGG	1 cut(s)	161
BstUI	CGCG	2 cut(s)	380, 403
BstV1I	GCAGC	3 cut(s)	144, 507, 547
BsuI	GTATCC	1 cut(s)	310
BsuRI	GGCC	2 cut(s)	18, 137
BtgI	CCRYGG	1 cut(s)	378
BtrI	CACGTC	1 cut(s)	352
BtsCI	GGATG	3 cut(s)	43, 181, 403
BtsIMutI	CAGTG	1 cut(s)	6
Cac8I	GCNNGC	4 cut(s)	405, 540, 552, 734
CciI	TCATGA	1 cut(s)	776
CfoI	GCGC	1 cut(s)	403
Cfr10I	RCCGGY	3 cut(s)	523, 550, 723
Cfr13I	GGNCC	2 cut(s)	16, 193
Cfr42I	CCGCGG	1 cut(s)	381
Csp6I	GTAC	2 cut(s)	256, 470
CviAII	CATG	1 cut(s)	777
CviQI	GTAC	2 cut(s)	256, 470
DdeI	CTNAG	1 cut(s)	327
DpnI	GATC	4 cut(s)	225, 347, 450, 765
DpnII	GATC	4 cut(s)	223, 345, 448, 763
EciI	GGCGGA	2 cut(s)	364, 650
Eco32I	GATATC	1 cut(s)	130
Eco47I	GGWCC	1 cut(s)	193
EcoRII	CCWGG	1 cut(s)	161
EcoRV	GATATC	1 cut(s)	130
FaeI	CATG	1 cut(s)	780
FaiI	YATR	7 cut(s)	58, 323, 389, 480, 482, 668, 778
FaqI	GGGAC	1 cut(s)	246
FatI	CATG	1 cut(s)	776
FauI	CCCGC	1 cut(s)	741
FauNDI	CATATG	1 cut(s)	480
FbaI	TGATCA	2 cut(s)	223, 448
Fnu4HI	GCNGC	6 cut(s)	158, 378, 381, 521, 536, 742
FokI	GGATG	3 cut(s)	50, 188, 410
Fsp4HI	GCNGC	6 cut(s)	158, 378, 381, 521, 536, 742
FspBI	CTAG	1 cut(s)	215
GlaI	GCGC	1 cut(s)	402
GluI	GCNGC	6 cut(s)	158, 378, 381, 521, 536, 742
HaeIII	GGCC	2 cut(s)	18, 137
HapII	CCGG	3 cut(s)	524, 551, 724
HhaI	GCGC	1 cut(s)	403
Hin1I	GRCGYC	1 cut(s)	246
Hin1II	CATG	1 cut(s)	780
Hin6I	GCGC	1 cut(s)	401
HinP1I	GCGC	1 cut(s)	401
HincII	GTYRAC	1 cut(s)	511
HindII	GTYRAC	1 cut(s)	511
HinfI	GANTC	1 cut(s)	364
HpaII	CCGG	3 cut(s)	524, 551, 724
HphI	GGTGA	1 cut(s)	598
Hpy166II	GTNNAC	1 cut(s)	511
Hpy188I	TCNGA	2 cut(s)	103, 655
Hpy188III	TCNNGA	2 cut(s)	329, 777
Hpy8I	GTNNAC	1 cut(s)	511
Hpy99I	CGWCG	6 cut(s)	83, 248, 251, 254, 518, 607
HpyCH4III	ACNGT	4 cut(s)	10, 62, 142, 148
HpyCH4IV	ACGT	3 cut(s)	81, 246, 351
HpyCH4V	TGCA	5 cut(s)	413, 478, 535, 679, 699
HpyF10VI	GCNNNNNNNGC	4 cut(s)	340, 676, 729, 738
HpyF3I	CTNAG	1 cut(s)	327
HpySE526I	ACGT	3 cut(s)	81, 246, 351
Hsp92I	GRCGYC	1 cut(s)	246
Hsp92II	CATG	1 cut(s)	780
HspAI	GCGC	1 cut(s)	401
KroI	GCCGGC	1 cut(s)	550
KroNI	GCCGGC	1 cut(s)	552
Ksp22I	TGATCA	2 cut(s)	223, 448
KspI	CCGCGG	1 cut(s)	381
Kzo9I	GATC	4 cut(s)	223, 345, 448, 763
LmnI	GCTCC	1 cut(s)	331
Lsp1109I	GCAGC	3 cut(s)	144, 507, 547
LweI	GCATC	2 cut(s)	388, 493
MaeI	CTAG	1 cut(s)	215
MaeII	ACGT	3 cut(s)	81, 246, 351
MaeIII	GTNAC	3 cut(s)	90, 142, 210
MalI	GATC	4 cut(s)	225, 347, 450, 765
MboI	GATC	4 cut(s)	223, 345, 448, 763
MboII	GAAGA	2 cut(s)	230, 555
MmeI	TCCRAC	2 cut(s)	168, 693
MroNI	GCCGGC	1 cut(s)	550
MspA1I	CMGCKG	2 cut(s)	380, 736
MspI	CCGG	3 cut(s)	524, 551, 724
MspR9I	CCNGG	1 cut(s)	163
Mva1269I	GAATGC	1 cut(s)	488
MvaI	CCWGG	1 cut(s)	163
MvnI	CGCG	2 cut(s)	380, 403
MwoI	GCNNNNNNNGC	4 cut(s)	340, 676, 729, 738
NaeI	GCCGGC	1 cut(s)	552
NdeI	CATATG	1 cut(s)	480
NdeII	GATC	4 cut(s)	223, 345, 448, 763
NgoMIV	GCCGGC	1 cut(s)	550
NlaIII	CATG	1 cut(s)	780
NlaIV	GGNNCC	1 cut(s)	652
PagI	TCATGA	1 cut(s)	776
PasI	CCCWGGG	1 cut(s)	162
PcsI	WCGNNNNNNNCGW	2 cut(s)	255, 400
PctI	GAATGC	1 cut(s)	488
PdiI	GCCGGC	1 cut(s)	552
PfeI	GAWTC	1 cut(s)	364
PflFI	GACNNNGTC	1 cut(s)	248
PflMI	CCANNNNNTGG	1 cut(s)	120
PkrI	GCNGC	6 cut(s)	159, 379, 382, 522, 537, 743
Psp6I	CCWGG	1 cut(s)	161
PspGI	CCWGG	1 cut(s)	161
PspN4I	GGNNCC	1 cut(s)	652
PspPI	GGNCC	2 cut(s)	16, 193
PsyI	GACNNNGTC	1 cut(s)	248
RsaI	GTAC	2 cut(s)	257, 471
RsaNI	GTAC	2 cut(s)	256, 470
SacII	CCGCGG	1 cut(s)	381
SatI	GCNGC	6 cut(s)	158, 378, 381, 521, 536, 742
Sau3AI	GATC	4 cut(s)	223, 345, 448, 763
Sau96I	GGNCC	2 cut(s)	16, 193
ScrFI	CCNGG	1 cut(s)	163
SetI	ASST	8 cut(s)	84, 249, 282, 336, 345, 354, 457, 522
SfaNI	GCATC	2 cut(s)	388, 493
Sfr303I	CCGCGG	1 cut(s)	381
SgrBI	CCGCGG	1 cut(s)	381
SinI	GGWCC	1 cut(s)	193
SmlI	CTYRAG	1 cut(s)	269
SmoI	CTYRAG	1 cut(s)	269
SsiI	CCGC	7 cut(s)	375, 378, 380, 506, 635, 734, 742
SspI	AATATT	1 cut(s)	110
SspMI	CTAG	1 cut(s)	215
StyD4I	CCNGG	1 cut(s)	161
TaaI	ACNGT	4 cut(s)	10, 62, 142, 148
TaiI	ACGT	3 cut(s)	84, 249, 354
TaqI	TCGA	6 cut(s)	78, 126, 252, 566, 623, 705
TatI	WGTACW	1 cut(s)	469
TauI	GCSGC	3 cut(s)	380, 383, 744
TfiI	GAWTC	1 cut(s)	364
TscAI	CASTG	1 cut(s)	13
TseI	GCWGC	3 cut(s)	157, 520, 535
TspDTI	ATGAA	1 cut(s)	481
TspGWI	ACGGA	1 cut(s)	344
TspRI	CASTG	1 cut(s)	13
Tth111I	GACNNNGTC	1 cut(s)	248
Van91I	CCANNNNNTGG	1 cut(s)	120
VpaK11BI	GGWCC	1 cut(s)	193
XcmI	CCANNNNNNNNNTGG	1 cut(s)	356
XspI	CTAG	1 cut(s)	215
ZraI	GACGTC	1 cut(s)	247

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.