Rroxscaffold_3G00224680

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000735
Physical Location & Seq
Reverse (-)
7921335 .. 7922250
916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224680.1

Sequence Viewer

Length: 690 bp
ATGGATCAGGAAATTGAGAATACTAGCACTGATTGCTGGTTGGAAAAGATCGAAGATCCTGAAATCGAGTTGGGTTGCACCAAGTTGGTGATGGAGATCGAGGCCACAATTACATGCCCAGGCAGATATTCGGAGGAAGAAGATGAAGATGATGAGGACTACTCCGATATCAATCAAATTCTAGCACAAGACAGCAACAGTTATGGGGACACACTCGAACTTAACTTCTTGATGGACGACAACAATGGAACCGCCCCGACTCGATTTATGAAGGAACAAATATCTAGCAAGCTCTTCGACTTGAATGTTCCCTTTAGGGATCATGAATCCATAGCAGACAAAATATTGAACGATGTCTTTGCGGTGGCAAGAGATCCTAGTCGTAAAGCCGACGGCAAGATTTTGTGTATGAGGGCCGTCATCCATGCTTTTGGGGAAGAATTGGGAGATGAGGATATGGACATTGACCCTAAATTTGTGCCTGCAAGTAAATCAGCTTTGGAGAAATTGGAGAGGGCGATCGTTGTCCAAGTCTCAACAATGTGTTCGGTATGTAGGGAGGAGCTTGTGGCTGGTTCCGAAGCAACTCGGATGCCGTGTTTGCATCTTTACCATGAGAGTTGCATTGTAGAGTGGTTGGGTAAGAGCAAGTACTGCCCGCTGTGTCGATATTCCATGCCAAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

229

Amino Acids

25.91

Weight (kDa)

4.34

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 178 - 223 2.7e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 181 - 223 1.3e-12 Ring finger domain
zf-C3HC4_2 PF13923 181 - 222 8.5e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 182 - 222 8.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 182 - 209 4.2e-06 RING-like zinc finger
zf-RING_UBOX PF13445 182 - 220 5.1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 252, 362, 659
AclWI GGATC 4 cut(s) 12, 50, 327, 368
AcsI RAATTY 2 cut(s) 177, 473
AfaI GTAC 1 cut(s) 653
AgsI TTSAA 2 cut(s) 304, 349
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 3 cut(s) 292, 497, 565
AluI AGCT 3 cut(s) 292, 497, 565
Alw26I GTCTC 1 cut(s) 538
AlwI GGATC 4 cut(s) 12, 50, 327, 368
AoxI GGCC 2 cut(s) 102, 414
ApoI RAATTY 2 cut(s) 177, 473
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AspS9I GGNCC 1 cut(s) 414
AsuHPI GGTGA 1 cut(s) 100
BccI CCATC 2 cut(s) 85, 226
BceAI ACGGC 3 cut(s) 401, 409, 580
BcgI CGANNNNNNTGC 4 cut(s) 277, 311, 341, 375
BciT130I CCWGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 538
BfaI CTAG 4 cut(s) 24, 182, 285, 378
BmcAI AGTACT 1 cut(s) 653
Bme1390I CCNGG 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 414
BmiI GGNNCC 2 cut(s) 250, 577
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 582, 613
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BsaBI GATNNNNATC 2 cut(s) 95, 171
BsaJI CCNNGG 1 cut(s) 118
Bse8I GATNNNNATC 2 cut(s) 95, 171
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 2 cut(s) 420, 597
BseJI GATNNNNATC 2 cut(s) 95, 171
BseRI GAGGAG 1 cut(s) 575
Bsh1285I CGRYCG 1 cut(s) 522
BshFI GGCC 2 cut(s) 104, 416
BsiEI CGRYCG 1 cut(s) 522
BslFI GGGAC 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 538
BsmFI GGGAC 1 cut(s) 221
BsnI GGCC 2 cut(s) 104, 416
Bsp143I GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
BspACI CCGC 3 cut(s) 252, 362, 659
BspANI GGCC 2 cut(s) 104, 416
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 250, 577
BspPI GGATC 4 cut(s) 12, 50, 327, 368
BspQI GCTCTTC 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 299
BstAPI GCANNNNNTGC 2 cut(s) 33, 654
BstC8I GCNNGC 3 cut(s) 290, 483, 659
BstF5I GGATG 2 cut(s) 420, 597
BstKTI GATC 7 cut(s) 7, 51, 58, 99, 322, 376, 522
BstMAI GTCTC 1 cut(s) 538
BstMBI GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
BstMCI CGRYCG 1 cut(s) 522
BstMWI GCNNNNNNNGC 3 cut(s) 33, 601, 654
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 118
BstX2I RGATCY 2 cut(s) 55, 373
BstXI CCANNNNNNTGG 1 cut(s) 431
BstYI RGATCY 2 cut(s) 55, 373
BsuRI GGCC 2 cut(s) 104, 416
BtsCI GGATG 2 cut(s) 420, 597
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 3 cut(s) 290, 483, 659
CciI TCATGA 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 414
Csp6I GTAC 1 cut(s) 652
CviAII CATG 5 cut(s) 114, 323, 425, 614, 676
CviJI RGCY 7 cut(s) 104, 292, 389, 416, 497, 565, 572
CviKI_1 RGCY 7 cut(s) 104, 292, 389, 416, 497, 565, 572
CviQI GTAC 1 cut(s) 652
DpnI GATC 7 cut(s) 6, 50, 57, 98, 321, 375, 521
DpnII GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
Eco32I GATATC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 118
EcoRV GATATC 1 cut(s) 169
FaeI CATG 5 cut(s) 117, 326, 428, 617, 679
FaqI GGGAC 1 cut(s) 221
FatI CATG 5 cut(s) 113, 322, 424, 613, 675
FauI CCCGC 1 cut(s) 666
FokI GGATG 2 cut(s) 407, 604
FspBI CTAG 4 cut(s) 24, 182, 285, 378
HaeIII GGCC 2 cut(s) 104, 416
Hin1II CATG 5 cut(s) 117, 326, 428, 617, 679
HinfI GANTC 2 cut(s) 259, 326
HphI GGTGA 1 cut(s) 100
Hpy188I TCNGA 4 cut(s) 133, 166, 580, 591
Hpy188III TCNNGA 4 cut(s) 8, 59, 229, 323
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4V TGCA 4 cut(s) 78, 485, 604, 624
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 601, 654
Hsp92II CATG 5 cut(s) 117, 326, 428, 617, 679
Kzo9I GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 1 cut(s) 562
LpnPI CCDG 6 cut(s) 22, 72, 105, 132, 495, 558
LweI GCATC 2 cut(s) 582, 613
MaeI CTAG 4 cut(s) 24, 182, 285, 378
MalI GATC 7 cut(s) 6, 50, 57, 98, 321, 375, 521
MboI GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
MboII GAAGA 6 cut(s) 65, 149, 152, 158, 286, 449
MflI RGATCY 2 cut(s) 55, 373
MluCI AATT 6 cut(s) 12, 108, 177, 440, 473, 506
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 21
MnlI CCTC 7 cut(s) 94, 127, 148, 405, 445, 507, 553
MseI TTAA 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 661
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 33, 601, 654
NdeII GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
NlaIII CATG 5 cut(s) 117, 326, 428, 617, 679
NlaIV GGNNCC 2 cut(s) 250, 577
NspI RCATGY 1 cut(s) 117
PagI TCATGA 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 299
PfeI GAWTC 1 cut(s) 326
Ple19I CGATCG 1 cut(s) 522
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 250, 577
PspPI GGNCC 1 cut(s) 414
PsuI RGATCY 2 cut(s) 55, 373
PvuI CGATCG 1 cut(s) 522
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 222
Sau3AI GATC 7 cut(s) 4, 48, 55, 96, 319, 373, 519
Sau96I GGNCC 1 cut(s) 414
ScaI AGTACT 1 cut(s) 653
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 120
SetI ASST 3 cut(s) 294, 499, 567
SfaNI GCATC 2 cut(s) 582, 613
Sse9I AATT 6 cut(s) 12, 108, 177, 440, 473, 506
SsiI CCGC 3 cut(s) 252, 362, 659
SspI AATATT 1 cut(s) 345
SspMI CTAG 4 cut(s) 24, 182, 285, 378
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 200
TaqI TCGA 7 cut(s) 51, 66, 99, 216, 262, 297, 667
TasI AATT 6 cut(s) 12, 108, 177, 440, 473, 506
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 34
TspDTI ATGAA 3 cut(s) 159, 284, 339
TspRI CASTG 1 cut(s) 34
XapI RAATTY 2 cut(s) 177, 473
XceI RCATGY 1 cut(s) 117
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 4 cut(s) 24, 182, 285, 378
ZrmI AGTACT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.