pycom07g06070

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000735
Physical Location & Seq
Forward (+)
5368295 .. 5368972
678 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06070.1

Sequence Viewer

Length: 678 bp
ATGGAAGCTGTAAGTGATCGGCAACTGGTGGGATACCATTGCGAATCGAAGCAGGCAGAGGTTTTTGAACCTGACAACGAATTGCCATGGACCCAATTCTTGCTGGGTGTCGATGCCAGAATACTGACGAGTCAGCCTGCCTTGCATTCCGAGGACGACGACGACTACGATTCACTTCCAGTAGAAGAAACCTACTTCGACGAACTCGAACTCGATTTGTTGATGGATGAAGACAAGTCAAGGGCTGCCCTAGCTGAGGCCCTGGTTTCTAGCCTGCATGTCCCTCCTCAGATTTGCGACTCCGTGGTGGATAAAATAGTACAAGATTTCCGAGTCGCAGAAACTGATTCTCGACCTTTCAGTGATCGCATGCTTGTTCATATGCCAGTGTCCATCGTTGTTCTTGTCGATGAATTTGGGGGCCTGGACACGGATGACCCTAAATTTGTGCCTGCAAGTAAGTCATCGATCGAGAAGCTGGAAAGGGTGAGAGTGGAAGTATCAGCGGATTGTTCAATTTGCATGGAGGAGATGGAGGTTGGTTCGGAAGCAATCCCCATGCCGTGCTCGCGCTCGCATCTTTACCACGACGATTGCATTATCGAGTGGTTGAAGAAAAGTAGGGTTTGCCCACTGTGCCGCTTCGCCATGCCTCCCGAAATAACAACACAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_3 zf-RING_2

Protein Analysis

226

Amino Acids

25.41

Weight (kDa)

4.31

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 170 - 214 2e-13 Ring finger domain
zf-RING_11 PF17123 170 - 200 3.8e-08 RING-like zinc finger
zf-rbx1 PF12678 171 - 214 3.7e-06 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 171 - 213 2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 171 - 213 8.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 571
AciI CCGC 2 cut(s) 506, 640
AcsI RAATTY 2 cut(s) 413, 443
AfaI GTAC 1 cut(s) 321
AfiI CCNNNNNNNGG 2 cut(s) 256, 430
AgsI TTSAA 3 cut(s) 68, 516, 613
AjnI CCWGG 2 cut(s) 261, 423
AluBI AGCT 3 cut(s) 8, 254, 478
AluI AGCT 3 cut(s) 8, 254, 478
Alw21I GWGCWC 1 cut(s) 569
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 2 cut(s) 258, 421
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 2 cut(s) 413, 443
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 3 cut(s) 90, 259, 421
AsuHPI GGTGA 1 cut(s) 499
AvaII GGWCC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 569
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 232
BccI CCATC 3 cut(s) 217, 401, 526
BceAI ACGGC 1 cut(s) 547
BciT130I CCWGG 2 cut(s) 263, 425
BciVI GTATCC 1 cut(s) 26
BfaI CTAG 2 cut(s) 251, 270
BfuI GTATCC 1 cut(s) 26
BisI GCNGC 2 cut(s) 246, 640
BlsI GCNGC 2 cut(s) 247, 641
Bme1390I CCNGG 2 cut(s) 263, 425
Bme18I GGWCC 1 cut(s) 90
BmgT120I GGNCC 3 cut(s) 90, 259, 421
BmiI GGNNCC 2 cut(s) 92, 422
BmrFI CCNGG 2 cut(s) 263, 425
BmsI GCATC 2 cut(s) 103, 586
BpiI GAAGAC 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 86, 150, 261, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 430
Bse1I ACTGG 3 cut(s) 30, 179, 386
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 2 cut(s) 263, 425
BseCI ATCGAT 1 cut(s) 467
BseDI CCNNGG 4 cut(s) 86, 150, 261, 303
BseGI GGATG 2 cut(s) 232, 439
BseLI CCNNNNNNNGG 2 cut(s) 256, 430
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 2 cut(s) 246, 302
BseNI ACTGG 3 cut(s) 30, 179, 386
BseRI GAGGAG 2 cut(s) 276, 542
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 103
Bsh1236I CGCG 1 cut(s) 571
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 2 cut(s) 260, 423
BshVI ATCGAT 1 cut(s) 467
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 569
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 256, 430
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 2 cut(s) 260, 423
Bsp1286I GDGCHC 1 cut(s) 569
Bsp143I GATC 3 cut(s) 16, 364, 468
Bsp19I CCATGG 1 cut(s) 86
BspACI CCGC 2 cut(s) 506, 640
BspANI GGCC 2 cut(s) 260, 423
BspCNI CTCAG 2 cut(s) 247, 301
BspDI ATCGAT 1 cut(s) 467
BspFNI CGCG 1 cut(s) 571
BspLI GGNNCC 2 cut(s) 92, 422
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 3 cut(s) 30, 179, 386
BssECI CCNNGG 4 cut(s) 86, 150, 261, 303
BssMI GATC 3 cut(s) 16, 364, 468
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 263, 425
Bst4CI ACNGT 1 cut(s) 636
BstC8I GCNNGC 7 cut(s) 54, 138, 275, 371, 453, 569, 575
BstDEI CTNAG 2 cut(s) 255, 288
BstDSI CCRYGG 2 cut(s) 86, 303
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 2 cut(s) 232, 439
BstFNI CGCG 1 cut(s) 571
BstHHI GCGC 1 cut(s) 573
BstKTI GATC 3 cut(s) 19, 367, 471
BstMBI GATC 3 cut(s) 16, 364, 468
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 4 cut(s) 142, 251, 568, 636
BstNI CCWGG 2 cut(s) 263, 425
BstNSI RCATGY 2 cut(s) 281, 373
BstSCI CCNGG 2 cut(s) 261, 423
BstUI CGCG 1 cut(s) 571
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 237
Bsu15I ATCGAT 1 cut(s) 467
BsuI GTATCC 1 cut(s) 26
BsuRI GGCC 2 cut(s) 260, 423
BsuTUI ATCGAT 1 cut(s) 467
BtgI CCRYGG 2 cut(s) 86, 303
BtsCI GGATG 2 cut(s) 232, 439
BtsIMutI CAGTG 3 cut(s) 367, 393, 632
Cac8I GCNNGC 7 cut(s) 54, 138, 275, 371, 453, 569, 575
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 1 cut(s) 573
Cfr13I GGNCC 3 cut(s) 90, 259, 421
ClaI ATCGAT 1 cut(s) 467
Csp6I GTAC 1 cut(s) 320
CspCI CAANNNNNGTGG 2 cut(s) 575, 610
CviAII CATG 6 cut(s) 87, 278, 370, 523, 559, 649
CviJI RGCY 8 cut(s) 8, 136, 245, 254, 260, 273, 423, 478
CviKI_1 RGCY 8 cut(s) 8, 136, 245, 254, 260, 273, 423, 478
CviQI GTAC 1 cut(s) 320
DdeI CTNAG 2 cut(s) 255, 288
DpnI GATC 3 cut(s) 18, 366, 470
DpnII GATC 3 cut(s) 16, 364, 468
Eco130I CCWWGG 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 90
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoO109I RGGNCCY 2 cut(s) 259, 421
EcoRII CCWGG 2 cut(s) 261, 423
EcoT14I CCWWGG 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 86
FaeI CATG 6 cut(s) 90, 281, 373, 526, 562, 652
FaiI YATR 8 cut(s) 88, 279, 371, 381, 383, 524, 560, 650
FaqI GGGAC 1 cut(s) 266
FatI CATG 6 cut(s) 86, 277, 369, 522, 558, 648
FauNDI CATATG 1 cut(s) 381
Fnu4HI GCNGC 2 cut(s) 246, 640
FokI GGATG 2 cut(s) 239, 446
Fsp4HI GCNGC 2 cut(s) 246, 640
FspBI CTAG 2 cut(s) 251, 270
GlaI GCGC 1 cut(s) 572
GluI GCNGC 2 cut(s) 246, 640
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 2 cut(s) 260, 423
HhaI GCGC 1 cut(s) 573
Hin1II CATG 6 cut(s) 90, 281, 373, 526, 562, 652
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HinfI GANTC 6 cut(s) 44, 130, 170, 299, 333, 347
HphI GGTGA 1 cut(s) 499
Hpy188I TCNGA 4 cut(s) 151, 291, 332, 547
Hpy188III TCNNGA 3 cut(s) 351, 472, 656
Hpy99I CGWCG 4 cut(s) 161, 164, 203, 593
HpyCH4III ACNGT 1 cut(s) 636
HpyCH4V TGCA 5 cut(s) 145, 277, 455, 522, 597
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 251, 568, 636
HpyF3I CTNAG 2 cut(s) 255, 288
Hsp92II CATG 6 cut(s) 90, 281, 373, 526, 562, 652
HspAI GCGC 1 cut(s) 571
Kzo9I GATC 3 cut(s) 16, 364, 468
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 2 cut(s) 103, 586
MaeI CTAG 2 cut(s) 251, 270
MalI GATC 3 cut(s) 18, 366, 470
MboI GATC 3 cut(s) 16, 364, 468
MboII GAAGA 3 cut(s) 197, 242, 625
MhlI GDGCHC 1 cut(s) 569
MluCI AATT 5 cut(s) 80, 95, 413, 443, 516
MlyI GAGTC 3 cut(s) 139, 293, 342
MnlI CCTC 8 cut(s) 52, 145, 250, 294, 297, 520, 529, 663
MspA1I CMGCKG 1 cut(s) 506
MspR9I CCNGG 2 cut(s) 263, 425
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 2 cut(s) 263, 425
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 4 cut(s) 142, 251, 568, 636
NcoI CCATGG 1 cut(s) 86
NdeI CATATG 1 cut(s) 381
NdeII GATC 3 cut(s) 16, 364, 468
NlaIII CATG 6 cut(s) 90, 281, 373, 526, 562, 652
NlaIV GGNNCC 2 cut(s) 92, 422
NspI RCATGY 2 cut(s) 281, 373
PaeI GCATGC 1 cut(s) 373
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 204
PctI GAATGC 1 cut(s) 145
PfeI GAWTC 3 cut(s) 44, 170, 347
PkrI GCNGC 2 cut(s) 247, 641
Ple19I CGATCG 1 cut(s) 471
PleI GAGTC 3 cut(s) 138, 293, 341
PpsI GAGTC 3 cut(s) 138, 293, 341
Psp6I CCWGG 2 cut(s) 261, 423
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 2 cut(s) 261, 423
PspN4I GGNNCC 2 cut(s) 92, 422
PspPI GGNCC 3 cut(s) 90, 259, 421
PstNI CAGNNNCTG 1 cut(s) 344
PvuI CGATCG 1 cut(s) 471
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
SatI GCNGC 2 cut(s) 246, 640
Sau3AI GATC 3 cut(s) 16, 364, 468
Sau96I GGNCC 3 cut(s) 90, 259, 421
SchI GAGTC 3 cut(s) 139, 293, 342
ScrFI CCNGG 2 cut(s) 263, 425
SduI GDGCHC 1 cut(s) 569
SetI ASST 8 cut(s) 10, 63, 73, 194, 256, 358, 480, 540
SfaNI GCATC 2 cut(s) 103, 586
SinI GGWCC 1 cut(s) 90
SphI GCATGC 1 cut(s) 373
Sse9I AATT 5 cut(s) 80, 95, 413, 443, 516
SsiI CCGC 2 cut(s) 506, 640
SspMI CTAG 2 cut(s) 251, 270
StyD4I CCNGG 2 cut(s) 261, 423
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 1 cut(s) 636
TasI AATT 5 cut(s) 80, 95, 413, 443, 516
TatI WGTACW 1 cut(s) 319
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 3 cut(s) 44, 170, 347
TscAI CASTG 3 cut(s) 367, 393, 639
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 3 cut(s) 243, 368, 426
TspGWI ACGGA 2 cut(s) 292, 446
TspRI CASTG 3 cut(s) 367, 393, 639
VpaK11BI GGWCC 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 2 cut(s) 413, 443
XceI RCATGY 2 cut(s) 281, 373
XspI CTAG 2 cut(s) 251, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.