Rroxscaffold_1G00022060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000735
Physical Location & Seq
Forward (+)
27147057 .. 27153021
5965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022060.1

Sequence Viewer

Length: 681 bp
ATGCGTGGTGCCACATTCGACGAGCTTCAACACTTGCAAGTCTCAATGGATGCTCGAAGGTCCATGGTGGACAAGATACTGAGCCAAGCTTTTCAGATGGCAGTGGCACATATTCCTGATGCCAGTCACAAGATTTTGCGTATGGCAGTGGCGGTCAGTATTTGTTTTGTTGATAATGATGATGACGACGATACGGAGGCCGATGATGAGACTGAGGATGATAATGATGATGGTATTGGTTATTACGAGCCTAGGTTTGTGCCGGCATGTAACTTGTTCCTTGAGAAACTGGAGAGGACGAGATCGAGCCGAAGTGTCAACAATGTGCTCCATTTGTATGGAGGAGATCTTGATATACTGAGAGATACTTATGTAGGGCAGACGTACCATGTGTTGGTGGGGCTGACCGGTCAATACAAATGCATGGGGTGTTTTGGAAAACATGTTGCTATTTCCGGTCAGCAATGTAAACATGAGGGTGAGGTTTTTTCATCGCCGAATAAGCTCCTGCTTAGTTTTACTAACGAGATTAATGGAATTGTTACTATGAAGCGGAGGGCTGGAAGGCTTAGAAAGAAGTATAAGGTCTCTCCTGCTCATAAGAGCACCTCTAACGGAGTGAGAATTCTAGATTGGTGTAGACCCGTACCCTTTGAGATACTTTCACTTGTGAATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

25.4

Weight (kDa)

6.06

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AccB7I CCANNNNNTGG 1 cut(s) 394
AccI GTMKAC 1 cut(s) 640
AciI CCGC 2 cut(s) 152, 553
AcsI RAATTY 1 cut(s) 624
AfaI GTAC 2 cut(s) 386, 648
AfiI CCNNNNNNNGG 1 cut(s) 394
AflIII ACRYGT 1 cut(s) 442
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 1 cut(s) 29
AjuI GAANNNNNNNTTGG 2 cut(s) 616, 648
AluBI AGCT 3 cut(s) 25, 89, 505
AluI AGCT 3 cut(s) 25, 89, 505
Alw21I GWGCWC 2 cut(s) 330, 608
Alw26I GTCTC 3 cut(s) 46, 203, 592
AoxI GGCC 1 cut(s) 198
ApoI RAATTY 1 cut(s) 624
AseI ATTAAT 1 cut(s) 531
AsiGI ACCGGT 1 cut(s) 407
AspA2I CCTAGG 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 491
AvaII GGWCC 1 cut(s) 60
AvrII CCTAGG 1 cut(s) 251
BaeI ACNNNNGTAYC 2 cut(s) 357, 390
BanI GGYRCC 1 cut(s) 8
Bbv12I GWGCWC 2 cut(s) 330, 608
BccI CCATC 2 cut(s) 91, 224
BcoDI GTCTC 3 cut(s) 46, 203, 592
BfaI CTAG 2 cut(s) 252, 629
BglII AGATCT 1 cut(s) 346
BlnI CCTAGG 1 cut(s) 251
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 10
BmsI GCATC 2 cut(s) 40, 109
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 2 cut(s) 63, 251
BsaWI WCCGGW 2 cut(s) 407, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 2 cut(s) 262, 407
Bse1I ACTGG 2 cut(s) 123, 294
Bse3DI GCAATG 1 cut(s) 470
BseDI CCNNGG 2 cut(s) 63, 251
BseGI GGATG 2 cut(s) 55, 223
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 1 cut(s) 470
BseMII CTCAG 3 cut(s) 71, 204, 350
BseNI ACTGG 2 cut(s) 123, 294
BseRI GAGGAG 1 cut(s) 357
BshFI GGCC 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 8
BshTI ACCGGT 1 cut(s) 407
BsiHKAI GWGCWC 2 cut(s) 330, 608
BsiSI CCGG 3 cut(s) 263, 408, 456
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 3 cut(s) 46, 203, 592
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 1 cut(s) 200
Bso31I GGTCTC 1 cut(s) 592
Bsp1286I GDGCHC 2 cut(s) 330, 608
Bsp143I GATC 2 cut(s) 302, 346
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 2 cut(s) 152, 553
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 3 cut(s) 72, 205, 351
BspLI GGNNCC 1 cut(s) 10
BspT107I GGYRCC 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 470
BsrFI RCCGGY 2 cut(s) 262, 407
BsrI ACTGG 2 cut(s) 123, 294
BssAI RCCGGY 2 cut(s) 262, 407
BssECI CCNNGG 2 cut(s) 63, 251
BssMI GATC 2 cut(s) 302, 346
BssT1I CCWWGG 2 cut(s) 63, 251
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 5 cut(s) 80, 213, 359, 512, 569
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 55, 223
BstKTI GATC 2 cut(s) 305, 349
BstMAI GTCTC 3 cut(s) 46, 203, 592
BstMBI GATC 2 cut(s) 302, 346
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstNSI RCATGY 2 cut(s) 270, 446
BstX2I RGATCY 1 cut(s) 346
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 1 cut(s) 346
BsuRI GGCC 1 cut(s) 200
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 55, 223
BtsI GCAGTG 2 cut(s) 108, 153
BtsIMutI CAGTG 2 cut(s) 108, 153
Cac8I GCNNGC 1 cut(s) 264
Cfr10I RCCGGY 2 cut(s) 262, 407
Cfr13I GGNCC 1 cut(s) 60
Csp6I GTAC 2 cut(s) 385, 647
CspAI ACCGGT 1 cut(s) 407
CviAII CATG 6 cut(s) 64, 267, 389, 424, 443, 473
CviQI GTAC 2 cut(s) 385, 647
DdeI CTNAG 5 cut(s) 80, 213, 359, 512, 569
DpnI GATC 2 cut(s) 304, 348
DpnII GATC 2 cut(s) 302, 346
Eco130I CCWWGG 2 cut(s) 63, 251
Eco31I GGTCTC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 624
EcoT14I CCWWGG 2 cut(s) 63, 251
EcoT22I ATGCAT 2 cut(s) 425, 679
ErhI CCWWGG 2 cut(s) 63, 251
FaeI CATG 6 cut(s) 67, 270, 392, 427, 446, 476
FatI CATG 6 cut(s) 63, 266, 388, 423, 442, 472
FblI GTMKAC 1 cut(s) 640
FokI GGATG 2 cut(s) 62, 230
FspBI CTAG 2 cut(s) 252, 629
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 1 cut(s) 200
HapII CCGG 3 cut(s) 263, 408, 456
Hin1II CATG 6 cut(s) 67, 270, 392, 427, 446, 476
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 87
HpaII CCGG 3 cut(s) 263, 408, 456
HphI GGTGA 1 cut(s) 491
Hpy166II GTNNAC 4 cut(s) 70, 319, 470, 641
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 3 cut(s) 116, 350, 629
Hpy8I GTNNAC 4 cut(s) 70, 319, 470, 641
Hpy99I CGWCG 2 cut(s) 23, 191
HpyAV CCTTC 2 cut(s) 51, 558
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 3 cut(s) 37, 423, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpyF3I CTNAG 5 cut(s) 80, 213, 359, 512, 569
HpySE526I ACGT 1 cut(s) 383
Hsp92II CATG 6 cut(s) 67, 270, 392, 427, 446, 476
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Kzo9I GATC 2 cut(s) 302, 346
LmnI GCTCC 2 cut(s) 333, 510
LpnPI CCDG 9 cut(s) 129, 136, 275, 276, 421, 469, 521, 546, 606
LweI GCATC 2 cut(s) 40, 109
MaeI CTAG 2 cut(s) 252, 629
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 3 cut(s) 125, 269, 541
MalI GATC 2 cut(s) 304, 348
MboI GATC 2 cut(s) 302, 346
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 330, 608
MluCI AATT 2 cut(s) 537, 624
MnlI CCTC 8 cut(s) 190, 208, 288, 335, 469, 475, 549, 619
Mph1103I ATGCAT 2 cut(s) 425, 679
MroNI GCCGGC 1 cut(s) 262
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 2 cut(s) 336, 477
MspI CCGG 3 cut(s) 263, 408, 456
Mva1269I GAATGC 1 cut(s) 679
MwoI GCNNNNNNNGC 1 cut(s) 502
NaeI GCCGGC 1 cut(s) 264
NcoI CCATGG 1 cut(s) 63
NdeII GATC 2 cut(s) 302, 346
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 6 cut(s) 67, 270, 392, 427, 446, 476
NlaIV GGNNCC 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 125
NsiI ATGCAT 2 cut(s) 425, 679
NspI RCATGY 2 cut(s) 270, 446
PciI ACATGT 1 cut(s) 442
PctI GAATGC 1 cut(s) 679
PdiI GCCGGC 1 cut(s) 264
PflMI CCANNNNNTGG 1 cut(s) 394
PinAI ACCGGT 1 cut(s) 407
PscI ACATGT 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 531
PspN4I GGNNCC 1 cut(s) 10
PspPI GGNCC 1 cut(s) 60
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 2 cut(s) 386, 648
RsaNI GTAC 2 cut(s) 385, 647
RseI CAYNNNNRTG 2 cut(s) 336, 477
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 2 cut(s) 302, 346
Sau96I GGNCC 1 cut(s) 60
SduI GDGCHC 2 cut(s) 330, 608
SetI ASST 9 cut(s) 27, 62, 91, 257, 386, 486, 507, 588, 611
SfaNI GCATC 2 cut(s) 40, 109
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 336, 477
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 2 cut(s) 537, 624
SsiI CCGC 2 cut(s) 152, 553
SspMI CTAG 2 cut(s) 252, 629
StyI CCWWGG 2 cut(s) 63, 251
TaiI ACGT 1 cut(s) 386
TaqI TCGA 3 cut(s) 18, 55, 305
TasI AATT 2 cut(s) 537, 624
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 2 cut(s) 108, 153
TseFI GTSAC 1 cut(s) 125
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 480, 563
TspGWI ACGGA 2 cut(s) 209, 630
TspRI CASTG 2 cut(s) 108, 153
Van91I CCANNNNNTGG 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 60
VspI ATTAAT 1 cut(s) 531
XapI RAATTY 1 cut(s) 624
XbaI TCTAGA 1 cut(s) 628
XceI RCATGY 2 cut(s) 270, 446
XmaJI CCTAGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 640
XspI CTAG 2 cut(s) 252, 629
Zsp2I ATGCAT 2 cut(s) 425, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.