Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000735

Physical Location & Seq

Genomic Coordinates Forward (+)

7349381 .. 7350055

Gene Structure 675 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1077200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 675 bp

ATGGAAGCTGTAAGTGATCGGCAAGTGGTGGGATACCATTGCGAATCGAAGCAGGCGGAGGTTTTTGAACCTGACAACGAATTGCCATGGACCCAATTCTTGCTGGGTGTCGATGCCAGAATATTGACAAGTCAGCCTGCCTTGCATTCCGAGGACGACGACGATTCACTTCCAGTAGAAGAAACCTACTTCGACGAACTCGAACTCGATTTGTTGATGGATGAAGACAAGTCAAGGGCTGCCCTAGCTGAGGCCCTGGTTTCTAGCCTGCATGTCCCTCCTCAGATTTGCCACTCCGTGGTGGATAAAATAGTACAAGATTTCCTAGTCGCAGAAACTGATTCTCGACCTTACAGTGATCGCATGCTTGTTCATATGCCAGTGTCCATCGTTGTTCTTGTCGATGAATTTGGAGGCCTGGACACGGATGACCCTAAATTTGTACCTGCAAGTAAGTCATCGATCGAGAAGCTGGAAAGGGTGAGAGTGGAAGTATCAGCAGATTGTTCAATTTGCATGGAGGAGATGGAGGTTGGTTCGGAAGCAATCCCCATGCCGTGCTCGCGCTCGCATCTTTACCACGACGACTGCATTCTCGAGTGGTTGAAGAAAAGTAGGGTTTGCCCACTGTGCCGCTTCGCCATGCCTCCCGAAATAATAACAACACAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBA_3 zf-RING_2

Protein Analysis

225

Amino Acids

25.23

Weight (kDa)

4.36

Isoelectric Point (pI)

47.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	168 - 212	1.2e-13	Ring finger domain
zf-RING_11	PF17123	168 - 198	3.7e-08	RING-like zinc finger
zf-rbx1	PF12678	169 - 212	1.5e-06	RING-H2 zinc finger domain
zf-C3HC4_2	PF13923	169 - 211	1.4e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	169 - 211	6.1e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000735)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g31481 FvH4_6g45550 FvH4_6g45551 FvH4_6g45560
malus_domestica	MD02G1237700.v1.1 MD02G1241100.v1.1 MD07G1074200.v1.1 MD07G1074300.v1.1 MD07G1077200.v1.1 MD09G1258700.v1.1
prunus_persica	Prupe.2G094100_v2.0.a1 Prupe.2G096300_v2.0.a1 Prupe.2G096400_v2.0.a1 Prupe.5G061200_v2.0.a1 Prupe.5G067900_v2.0.a1
pyrus_communis	pycom02g20840 pycom07g05830 pycom07g06070 pycom07g06080
rosa_chinensis	RchiOBHm_Chr1g0336601 RchiOBHm_Chr2g0163491 RchiOBHm_Chr5g0058471 RchiOBHm_Chr7g0236931
rosa_laevigata	RLG00000001047 RLG00000021406 RLG00000029344 RLG00000035224 RLG00000035225 RLG00000035226 RLG00000036678
rosa_multiflora	Rmu_co8414615.1_g000001 Rmu_sc0000367.1_g000124 Rmu_sc0000367.1_g000131 Rmu_sc0001969.1_g000007 Rmu_sc0004006.1_g000003 Rmu_sc0005576.1_g000003 Rmu_sc0008383.1_g000007 Rmu_sc0013124.1_g000008 Rmu_sc0024363.1_g000004 Rmu_ssc0000335.1_g000002
rosa_roxburghii	Rroxscaffold_1G00004750 Rroxscaffold_1G00022030 Rroxscaffold_1G00022050 Rroxscaffold_1G00022060 Rroxscaffold_2G00087240 Rroxscaffold_2G00087270 Rroxscaffold_3G00224680
rosa_rugosa	Rorug04G0097000 Rorug05G0315100 Rorug05G0315200 Rorug05G0315400 Rorug05G0453100 Rorug07G0301000
rosa_samantha	Rh1DG154700 Rh2AG570200 Rh2BG582400 Rh2CG552200 Rh4CG163700 Rh5AG381500 Rh5BG393100 Rh5CG417300 Rh5DG408300 Rh5DG408400 Rh5DG541900 Rh7AG456000 Rh7BG427300 Rh7CG474800 Rh7DG443900
rosa_wichuraiana	Rw1G011960 Rw2G047210 Rw5G035950 Rw7G037850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	454
AccB7I	CCANNNNNTGG	1 cut(s)	298
AccII	CGCG	1 cut(s)	565
AciI	CCGC	2 cut(s)	56, 634
AcsI	RAATTY	2 cut(s)	407, 437
AdeI	CACNNNGTG	1 cut(s)	298
AfaI	GTAC	2 cut(s)	315, 444
AfiI	CCNNNNNNNGG	3 cut(s)	250, 298, 424
AgsI	TTSAA	3 cut(s)	68, 510, 607
AjnI	CCWGG	2 cut(s)	255, 417
AluBI	AGCT	3 cut(s)	8, 248, 472
AluI	AGCT	3 cut(s)	8, 248, 472
Alw21I	GWGCWC	1 cut(s)	563
AlwNI	CAGNNNCTG	1 cut(s)	338
Ama87I	CYCGRG	1 cut(s)	596
AoxI	GGCC	2 cut(s)	252, 415
ApeKI	GCWGC	1 cut(s)	239
ApoI	RAATTY	2 cut(s)	407, 437
ArsI	GACNNNNNNTTYG	2 cut(s)	422, 454
AspLEI	GCGC	1 cut(s)	567
AspS9I	GGNCC	2 cut(s)	90, 253
AsuHPI	GGTGA	1 cut(s)	493
AvaI	CYCGRG	1 cut(s)	596
AvaII	GGWCC	1 cut(s)	90
BbsI	GAAGAC	1 cut(s)	231
Bbv12I	GWGCWC	1 cut(s)	563
BbvCI	CCTCAGC	1 cut(s)	249
BbvI	GCAGC	1 cut(s)	226
BccI	CCATC	3 cut(s)	211, 395, 520
BceAI	ACGGC	1 cut(s)	541
BciT130I	CCWGG	2 cut(s)	257, 419
BciVI	GTATCC	1 cut(s)	26
BfaI	CTAG	3 cut(s)	245, 264, 326
BfuAI	ACCTGC	1 cut(s)	454
BfuI	GTATCC	1 cut(s)	26
BisI	GCNGC	2 cut(s)	240, 634
BlsI	GCNGC	2 cut(s)	241, 635
Bme1390I	CCNGG	2 cut(s)	257, 419
Bme18I	GGWCC	1 cut(s)	90
BmeT110I	CYCGRG	1 cut(s)	596
BmgT120I	GGNCC	2 cut(s)	90, 253
BmiI	GGNNCC	1 cut(s)	92
BmrFI	CCNGG	2 cut(s)	257, 419
BmsI	GCATC	2 cut(s)	103, 580
BpiI	GAAGAC	1 cut(s)	231
Bpu10I	CCTNAGC	1 cut(s)	249
Bsa29I	ATCGAT	1 cut(s)	461
BsaJI	CCNNGG	4 cut(s)	86, 150, 255, 297
Bsc4I	CCNNNNNNNGG	3 cut(s)	250, 298, 424
Bse1I	ACTGG	2 cut(s)	173, 380
Bse3DI	GCAATG	1 cut(s)	37
BseBI	CCWGG	2 cut(s)	257, 419
BseCI	ATCGAT	1 cut(s)	461
BseDI	CCNNGG	4 cut(s)	86, 150, 255, 297
BseGI	GGATG	2 cut(s)	226, 433
BseLI	CCNNNNNNNGG	3 cut(s)	250, 298, 424
BseMI	GCAATG	1 cut(s)	37
BseMII	CTCAG	2 cut(s)	240, 296
BseNI	ACTGG	2 cut(s)	173, 380
BseRI	GAGGAG	2 cut(s)	270, 536
BseXI	GCAGC	1 cut(s)	226
BseYI	CCCAGC	1 cut(s)	103
Bsh1236I	CGCG	1 cut(s)	565
Bsh1285I	CGRYCG	1 cut(s)	465
BshFI	GGCC	2 cut(s)	254, 417
BshVI	ATCGAT	1 cut(s)	461
BsiEI	CGRYCG	1 cut(s)	465
BsiHKAI	GWGCWC	1 cut(s)	563
BsiHKCI	CYCGRG	1 cut(s)	596
BslFI	GGGAC	1 cut(s)	260
BslI	CCNNNNNNNGG	3 cut(s)	250, 298, 424
BsmFI	GGGAC	1 cut(s)	260
BsmI	GAATGC	2 cut(s)	145, 591
BsnI	GGCC	2 cut(s)	254, 417
BsoBI	CYCGRG	1 cut(s)	596
Bsp1286I	GDGCHC	1 cut(s)	563
Bsp143I	GATC	3 cut(s)	16, 358, 462
Bsp19I	CCATGG	1 cut(s)	86
BspACI	CCGC	2 cut(s)	56, 634
BspANI	GGCC	2 cut(s)	254, 417
BspCNI	CTCAG	2 cut(s)	241, 295
BspDI	ATCGAT	1 cut(s)	461
BspFNI	CGCG	1 cut(s)	565
BspLI	GGNNCC	1 cut(s)	92
BspMI	ACCTGC	1 cut(s)	454
BsrDI	GCAATG	1 cut(s)	37
BsrI	ACTGG	2 cut(s)	173, 380
BssECI	CCNNGG	4 cut(s)	86, 150, 255, 297
BssMI	GATC	3 cut(s)	16, 358, 462
BssT1I	CCWWGG	1 cut(s)	86
Bst2UI	CCWGG	2 cut(s)	257, 419
Bst4CI	ACNGT	2 cut(s)	356, 630
BstC8I	GCNNGC	6 cut(s)	54, 138, 269, 365, 563, 569
BstDEI	CTNAG	2 cut(s)	249, 282
BstDSI	CCRYGG	2 cut(s)	86, 297
BstENI	CCTNNNNNAGG	1 cut(s)	248
BstF5I	GGATG	2 cut(s)	226, 433
BstFNI	CGCG	1 cut(s)	565
BstHHI	GCGC	1 cut(s)	567
BstKTI	GATC	3 cut(s)	19, 361, 465
BstMBI	GATC	3 cut(s)	16, 358, 462
BstMCI	CGRYCG	1 cut(s)	465
BstMWI	GCNNNNNNNGC	4 cut(s)	142, 245, 562, 630
BstNI	CCWGG	2 cut(s)	257, 419
BstNSI	RCATGY	2 cut(s)	275, 367
BstSCI	CCNGG	2 cut(s)	255, 417
BstUI	CGCG	1 cut(s)	565
BstV1I	GCAGC	1 cut(s)	226
BstV2I	GAAGAC	1 cut(s)	231
Bsu15I	ATCGAT	1 cut(s)	461
BsuI	GTATCC	1 cut(s)	26
BsuRI	GGCC	2 cut(s)	254, 417
BsuTUI	ATCGAT	1 cut(s)	461
BtgI	CCRYGG	2 cut(s)	86, 297
BtsCI	GGATG	2 cut(s)	226, 433
BtsIMutI	CAGTG	3 cut(s)	361, 387, 626
BveI	ACCTGC	1 cut(s)	454
Cac8I	GCNNGC	6 cut(s)	54, 138, 269, 365, 563, 569
CaiI	CAGNNNCTG	1 cut(s)	338
CfoI	GCGC	1 cut(s)	567
Cfr13I	GGNCC	2 cut(s)	90, 253
ClaI	ATCGAT	1 cut(s)	461
Csp6I	GTAC	2 cut(s)	314, 443
CviAII	CATG	6 cut(s)	87, 272, 364, 517, 553, 643
CviJI	RGCY	8 cut(s)	8, 136, 239, 248, 254, 267, 417, 472
CviKI_1	RGCY	8 cut(s)	8, 136, 239, 248, 254, 267, 417, 472
CviQI	GTAC	2 cut(s)	314, 443
DdeI	CTNAG	2 cut(s)	249, 282
DpnI	GATC	3 cut(s)	18, 360, 464
DpnII	GATC	3 cut(s)	16, 358, 462
DraIII	CACNNNGTG	1 cut(s)	298
EciI	GGCGGA	1 cut(s)	71
Eco130I	CCWWGG	1 cut(s)	86
Eco147I	AGGCCT	1 cut(s)	417
Eco47I	GGWCC	1 cut(s)	90
Eco88I	CYCGRG	1 cut(s)	596
EcoNI	CCTNNNNNAGG	1 cut(s)	248
EcoO109I	RGGNCCY	1 cut(s)	253
EcoRII	CCWGG	2 cut(s)	255, 417
EcoT14I	CCWWGG	1 cut(s)	86
ErhI	CCWWGG	1 cut(s)	86
FaeI	CATG	6 cut(s)	90, 275, 367, 520, 556, 646
FaiI	YATR	8 cut(s)	88, 273, 365, 375, 377, 518, 554, 644
FaqI	GGGAC	1 cut(s)	260
FatI	CATG	6 cut(s)	86, 271, 363, 516, 552, 642
FauNDI	CATATG	1 cut(s)	375
Fnu4HI	GCNGC	2 cut(s)	240, 634
FokI	GGATG	2 cut(s)	233, 440
Fsp4HI	GCNGC	2 cut(s)	240, 634
FspBI	CTAG	3 cut(s)	245, 264, 326
GlaI	GCGC	1 cut(s)	566
GluI	GCNGC	2 cut(s)	240, 634
GsaI	CCCAGC	1 cut(s)	107
HaeIII	GGCC	2 cut(s)	254, 417
HhaI	GCGC	1 cut(s)	567
Hin1II	CATG	6 cut(s)	90, 275, 367, 520, 556, 646
Hin6I	GCGC	1 cut(s)	565
HinP1I	GCGC	1 cut(s)	565
HinfI	GANTC	3 cut(s)	44, 164, 341
HphI	GGTGA	1 cut(s)	493
Hpy188I	TCNGA	3 cut(s)	151, 285, 541
Hpy188III	TCNNGA	4 cut(s)	345, 466, 596, 650
Hpy99I	CGWCG	4 cut(s)	161, 164, 197, 587
HpyCH4III	ACNGT	2 cut(s)	356, 630
HpyCH4V	TGCA	5 cut(s)	145, 271, 449, 516, 591
HpyF10VI	GCNNNNNNNGC	4 cut(s)	142, 245, 562, 630
HpyF3I	CTNAG	2 cut(s)	249, 282
Hsp92II	CATG	6 cut(s)	90, 275, 367, 520, 556, 646
HspAI	GCGC	1 cut(s)	565
Kzo9I	GATC	3 cut(s)	16, 358, 462
Lsp1109I	GCAGC	1 cut(s)	226
LweI	GCATC	2 cut(s)	103, 580
MaeI	CTAG	3 cut(s)	245, 264, 326
MalI	GATC	3 cut(s)	18, 360, 464
MboI	GATC	3 cut(s)	16, 358, 462
MboII	GAAGA	3 cut(s)	191, 236, 619
MhlI	GDGCHC	1 cut(s)	563
MluCI	AATT	6 cut(s)	80, 95, 407, 437, 510, 670
MnlI	CCTC	9 cut(s)	52, 145, 244, 288, 291, 407, 514, 523, 657
MseI	TTAA	1 cut(s)	673
MspR9I	CCNGG	2 cut(s)	257, 419
Mva1269I	GAATGC	2 cut(s)	145, 591
MvaI	CCWGG	2 cut(s)	257, 419
MvnI	CGCG	1 cut(s)	565
MwoI	GCNNNNNNNGC	4 cut(s)	142, 245, 562, 630
NcoI	CCATGG	1 cut(s)	86
NdeI	CATATG	1 cut(s)	375
NdeII	GATC	3 cut(s)	16, 358, 462
NlaIII	CATG	6 cut(s)	90, 275, 367, 520, 556, 646
NlaIV	GGNNCC	1 cut(s)	92
NspI	RCATGY	2 cut(s)	275, 367
PaeI	GCATGC	1 cut(s)	367
PaeR7I	CTCGAG	1 cut(s)	596
PceI	AGGCCT	1 cut(s)	417
PcsI	WCGNNNNNNNCGW	1 cut(s)	198
PctI	GAATGC	2 cut(s)	145, 591
PfeI	GAWTC	3 cut(s)	44, 164, 341
PflMI	CCANNNNNTGG	1 cut(s)	298
PkrI	GCNGC	2 cut(s)	241, 635
Ple19I	CGATCG	1 cut(s)	465
Psp6I	CCWGG	2 cut(s)	255, 417
PspFI	CCCAGC	1 cut(s)	103
PspGI	CCWGG	2 cut(s)	255, 417
PspN4I	GGNNCC	1 cut(s)	92
PspPI	GGNCC	2 cut(s)	90, 253
PstNI	CAGNNNCTG	1 cut(s)	338
PvuI	CGATCG	1 cut(s)	465
RsaI	GTAC	2 cut(s)	315, 444
RsaNI	GTAC	2 cut(s)	314, 443
SaqAI	TTAA	1 cut(s)	673
SatI	GCNGC	2 cut(s)	240, 634
Sau3AI	GATC	3 cut(s)	16, 358, 462
Sau96I	GGNCC	2 cut(s)	90, 253
ScrFI	CCNGG	2 cut(s)	257, 419
SduI	GDGCHC	1 cut(s)	563
SetI	ASST	9 cut(s)	10, 63, 73, 188, 250, 352, 448, 474, 534
SfaNI	GCATC	2 cut(s)	103, 580
Sfr274I	CTCGAG	1 cut(s)	596
SinI	GGWCC	1 cut(s)	90
SlaI	CTCGAG	1 cut(s)	596
SmlI	CTYRAG	1 cut(s)	596
SmoI	CTYRAG	1 cut(s)	596
SphI	GCATGC	1 cut(s)	367
Sse9I	AATT	6 cut(s)	80, 95, 407, 437, 510, 670
SseBI	AGGCCT	1 cut(s)	417
SsiI	CCGC	2 cut(s)	56, 634
SspI	AATATT	1 cut(s)	123
SspMI	CTAG	3 cut(s)	245, 264, 326
StuI	AGGCCT	1 cut(s)	417
StyD4I	CCNGG	2 cut(s)	255, 417
StyI	CCWWGG	1 cut(s)	86
TaaI	ACNGT	2 cut(s)	356, 630
TasI	AATT	6 cut(s)	80, 95, 407, 437, 510, 670
TatI	WGTACW	1 cut(s)	313
TauI	GCSGC	1 cut(s)	636
TfiI	GAWTC	3 cut(s)	44, 164, 341
Tru1I	TTAA	1 cut(s)	673
Tru9I	TTAA	1 cut(s)	673
TscAI	CASTG	3 cut(s)	361, 387, 633
TseI	GCWGC	1 cut(s)	239
TspDTI	ATGAA	3 cut(s)	237, 362, 420
TspGWI	ACGGA	2 cut(s)	286, 440
TspRI	CASTG	3 cut(s)	361, 387, 633
Van91I	CCANNNNNTGG	1 cut(s)	298
VpaK11BI	GGWCC	1 cut(s)	90
XagI	CCTNNNNNAGG	1 cut(s)	248
XapI	RAATTY	2 cut(s)	407, 437
XceI	RCATGY	2 cut(s)	275, 367
XhoI	CTCGAG	1 cut(s)	596
XspI	CTAG	3 cut(s)	245, 264, 326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.